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Protein

Cytochrome c oxidase subunit 1+2

Gene

COX1/2

Organism
Acanthamoeba castellanii (Amoeba)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B (By similarity).By similarity

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Pathwayi: oxidative phosphorylation

This protein is involved in the pathway oxidative phosphorylation, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway oxidative phosphorylation and in Energy metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi75Iron (heme A axial ligand)Curated1
Metal bindingi254Copper BCurated1
Metal bindingi258Copper BCurated1
Metal bindingi303Copper BCurated1
Metal bindingi304Copper BCurated1
Metal bindingi389Iron (heme A3 axial ligand)Curated1
Metal bindingi391Iron (heme A axial ligand)Curated1
Metal bindingi801Copper ACurated1
Metal bindingi836Copper ACurated1
Metal bindingi840Copper ACurated1
Metal bindingi844Copper ACurated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00705.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 1+2 (EC:1.9.3.1)
Alternative name(s):
Cytochrome c oxidase polypeptide I+II
Gene namesi
Name:COX1/2
Encoded oniMitochondrion
OrganismiAcanthamoeba castellanii (Amoeba)
Taxonomic identifieri5755 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaDiscoseaLongamoebiaCentramoebidaAcanthamoebidaeAcanthamoeba

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei29 – 49HelicalSequence analysisAdd BLAST21
Transmembranei70 – 90HelicalSequence analysisAdd BLAST21
Transmembranei91 – 111HelicalSequence analysisAdd BLAST21
Transmembranei114 – 134HelicalSequence analysisAdd BLAST21
Transmembranei159 – 179HelicalSequence analysisAdd BLAST21
Transmembranei197 – 217HelicalSequence analysisAdd BLAST21
Transmembranei248 – 268HelicalSequence analysisAdd BLAST21
Transmembranei280 – 300HelicalSequence analysisAdd BLAST21
Transmembranei323 – 343HelicalSequence analysisAdd BLAST21
Transmembranei351 – 371HelicalSequence analysisAdd BLAST21
Transmembranei390 – 410HelicalSequence analysisAdd BLAST21
Transmembranei427 – 447HelicalSequence analysisAdd BLAST21
Transmembranei469 – 489HelicalSequence analysisAdd BLAST21
Transmembranei536 – 556HelicalSequence analysisAdd BLAST21
Transmembranei585 – 605HelicalSequence analysisAdd BLAST21
Transmembranei656 – 676HelicalSequence analysisAdd BLAST21
Transmembranei845 – 865HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001832751 – 873Cytochrome c oxidase subunit 1+2Add BLAST873

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki254 ↔ 2581'-histidyl-3'-tyrosine (His-Tyr)By similarity

Proteomic databases

PRIDEiQ37370.

Structurei

3D structure databases

ProteinModelPortaliQ37370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 474COX1Add BLAST474
Regioni475 – 843COX2Add BLAST369

Sequence similaritiesi

In the N-terminal section; belongs to the heme-copper respiratory oxidase family.Curated
In the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

CDDicd01663. Cyt_c_Oxidase_I. 1 hit.
Gene3Di1.10.287.90. 1 hit.
1.20.210.10. 1 hit.
2.60.40.420. 1 hit.
InterProiIPR001505. Copper_CuA.
IPR000883. COX1.
IPR008972. Cupredoxin.
IPR023616. Cyt_c_oxase-like_su1_dom.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR033944. Cyt_c_oxase_su1_dom.
IPR014222. Cyt_c_oxidase_su2.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 2 hits.
PfamiPF00115. COX1. 1 hit.
PF00116. COX2. 2 hits.
PF02790. COX2_TM. 2 hits.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF49503. SSF49503. 2 hits.
SSF81442. SSF81442. 1 hit.
SSF81464. SSF81464. 2 hits.
TIGRFAMsiTIGR02866. CoxB. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
PS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q37370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINRLLNNLT SFFTDNRWLF STNHKDIGTL YLIFGGFSGI IGTIFSMIIR
60 70 80 90 100
LELAAPGSQI LSGNSQLYNV IITAHAFVMI FFFVMPVMIG GFGNWFVPLM
110 120 130 140 150
IGAPDMAFPR LNNISFWLLP PSLFLLLCSS LVEFGAGTGW TVYPPLSSIV
160 170 180 190 200
AHSGGSVDLA IFSLHLAGIS SLLGAINFIT TIFNMRVPGL SMHKLPLFVW
210 220 230 240 250
SVLITAFLLL FSLPVLAGAI TMLLTDRNFN TSFFDPSGGG DPILYQHLFW
260 270 280 290 300
FFGHPEVYIL ILPAFGIVSQ IIGTFSNKSI FGYIGMVYAM LSIAVLGFIV
310 320 330 340 350
WAHHMYTVGL DVDTRAYFTA ATMMIAVPTG IKIFSWIATL WGGQIVRKTP
360 370 380 390 400
LLFVIGFLIL FTLGGLTGIV LSNAGLDIML HDTYYVVAHF HYVLSMGAVF
410 420 430 440 450
AFFAGFYYWF WKISGYTYNE MYGNVHFWLM FIGVNLTFFP MHFVGLAGMP
460 470 480 490 500
RRIPDYPDNY YYWNILSSFG SIISSVSVIV FFYLIYLAFN NNNTPKLIKL
510 520 530 540 550
VHSIFAPYIN TLSKNLLTFA SIKSTSDSSF FKFSKFFIFF MVSLSVLFIF
560 570 580 590 600
YDSLLCLNDH TNSWKIGFQD PTTPIAYGII KLHDHILFFL AVILFVVGYL
610 620 630 640 650
LLSTYKKFYY GSLNNDLPES KRISLFDTLI NTYKENLSFN VTNRTYNINH
660 670 680 690 700
GTTIEIIWTI LPAFILLFIA VPSFALLYAM DEIIDPVLTV KVIGHQWYWS
710 720 730 740 750
YEYSDYSVVY SNRMLDYDSI DRFAAMEMMY KGMGYLKDRS LLSYLYIPMV
760 770 780 790 800
IPETTIKFDS YMIHEAELNL GDLRLLKTDM PLFLPKNTHI RLLITSSDVL
810 820 830 840 850
HSWAVPSFGV KVDAVPGRLN QTSLYLKNTG TFYGQCSELC GVNHAFMPIE
860 870
VYVVNPVYFY NYVYIYFKNF NLI
Length:873
Mass (Da):99,214
Last modified:November 1, 1996 - v1
Checksum:i0BC820F0162B8EB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12386 Genomic DNA. Translation: AAD11820.1.
PIRiS53828.
RefSeqiNP_042527.1. NC_001637.1.

Genome annotation databases

GeneIDi1734020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12386 Genomic DNA. Translation: AAD11820.1.
PIRiS53828.
RefSeqiNP_042527.1. NC_001637.1.

3D structure databases

ProteinModelPortaliQ37370.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ37370.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1734020.

Enzyme and pathway databases

UniPathwayiUPA00705.

Family and domain databases

CDDicd01663. Cyt_c_Oxidase_I. 1 hit.
Gene3Di1.10.287.90. 1 hit.
1.20.210.10. 1 hit.
2.60.40.420. 1 hit.
InterProiIPR001505. Copper_CuA.
IPR000883. COX1.
IPR008972. Cupredoxin.
IPR023616. Cyt_c_oxase-like_su1_dom.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR033944. Cyt_c_oxase_su1_dom.
IPR014222. Cyt_c_oxidase_su2.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 2 hits.
PfamiPF00115. COX1. 1 hit.
PF00116. COX2. 2 hits.
PF02790. COX2_TM. 2 hits.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF49503. SSF49503. 2 hits.
SSF81442. SSF81442. 1 hit.
SSF81464. SSF81464. 2 hits.
TIGRFAMsiTIGR02866. CoxB. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
PS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOX1_ACACA
AccessioniPrimary (citable) accession number: Q37370
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.