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Protein

Calcium-transporting ATPase 1

Gene

ACA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulationi

Activated by calmodulin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4564-aspartylphosphate intermediateBy similarity1
Metal bindingi758MagnesiumBy similarity1
Metal bindingi762MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium channel activity Source: TAIR
  • calcium-transporting ATPase activity Source: TAIR
  • calmodulin binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

Keywordsi

Molecular functionCalmodulin-binding, Hydrolase
Biological processCalcium transport, Ion transport, Transport
LigandATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G27770-MONOMER
MetaCyc:MONOMER-14612
ReactomeiR-ATH-418359 Reduction of cytosolic Ca++ levels
R-ATH-5578775 Ion homeostasis
R-ATH-936837 Ion transport by P-type ATPases

Protein family/group databases

TCDBi3.A.3.2.11 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 1 (EC:3.6.3.8)
Alternative name(s):
Ca(2+)-ATPase isoform 1
Plastid envelope ATPase 1
Gene namesi
Name:ACA1
Synonyms:PEA1
Ordered Locus Names:At1g27770
ORF Names:F28L5.1, T22C5.23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G27770
TAIRilocus:2029794 AT1G27770

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 162StromalSequence analysisAdd BLAST162
Transmembranei163 – 183HelicalSequence analysisAdd BLAST21
Topological domaini184 – 201LumenalSequence analysisAdd BLAST18
Transmembranei202 – 222HelicalSequence analysisAdd BLAST21
Topological domaini223 – 350StromalSequence analysisAdd BLAST128
Transmembranei351 – 370HelicalSequence analysisAdd BLAST20
Topological domaini371 – 400LumenalSequence analysisAdd BLAST30
Transmembranei401 – 418HelicalSequence analysisAdd BLAST18
Topological domaini419 – 813StromalSequence analysisAdd BLAST395
Transmembranei814 – 832HelicalSequence analysisAdd BLAST19
Topological domaini833 – 843LumenalSequence analysisAdd BLAST11
Transmembranei844 – 864HelicalSequence analysisAdd BLAST21
Topological domaini865 – 884StromalSequence analysisAdd BLAST20
Transmembranei885 – 907HelicalSequence analysisAdd BLAST23
Topological domaini908 – 919LumenalSequence analysisAdd BLAST12
Transmembranei920 – 941HelicalSequence analysisAdd BLAST22
Topological domaini942 – 959StromalSequence analysisAdd BLAST18
Transmembranei960 – 981HelicalSequence analysisAdd BLAST22
Topological domaini982 – 991LumenalSequence analysis10
Transmembranei992 – 1013HelicalSequence analysisAdd BLAST22
Topological domaini1014 – 1020StromalSequence analysis7

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Plastid inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464101 – 1020Calcium-transporting ATPase 1Add BLAST1020

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei46Phosphoserine; by CPKBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ37145

PTM databases

iPTMnetiQ37145
SwissPalmiQ37145

Expressioni

Tissue specificityi

Expressed at higher levels in roots than in leaves.

Gene expression databases

ExpressionAtlasiQ37145 baseline and differential
GenevisibleiQ37145 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT1G27770.1

Structurei

3D structure databases

ProteinModelPortaliQ37145
SMRiQ37145
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 32Interaction with calmodulinBy similarityAdd BLAST12

Domaini

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204 Eukaryota
ENOG410XNNC LUCA
HOGENOMiHOG000265623
InParanoidiQ37145
KOiK01537
OMAiNAVDCNQ
OrthoDBiEOG093600UZ
PhylomeDBiQ37145

Family and domain databases

Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR024750 Ca_ATPase_N_dom
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006408 P-type_ATPase_IIB
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF12515 CaATP_NAI, 1 hit
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
PRINTSiPR00120 HATPASE
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 2 hits
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01517 ATPase-IIB_Ca, 1 hit
TIGR01494 ATPase_P-type, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q37145-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESYLNENFG DVKPKNSSDE ALQRWRKLCW IVKNPKRRFR FTANLSKRSE
60 70 80 90 100
AEAIRRSNQE KFRVAVLVSQ AALQFINSLK LSSEYTLPEE VRKAGFEICP
110 120 130 140 150
DELGSIVEGH DLKKLKIHGG TEGLTEKLST SIASGISTSE DLLSVRKEIY
160 170 180 190 200
GINQFTESPS RGFWLFVWEA LQDTTLMILA ACAFVSLIVG ILMEGWPIGA
210 220 230 240 250
HDGLGIVASI LLVVFVTATS DYRQSLQFKD LDAEKKKIVV QVTRDKLRQK
260 270 280 290 300
ISIYDLLPGD VVHLGIGDQI PADGLFISGF SVLINESSLT GESEPVSVSV
310 320 330 340 350
EHPFLLSGTK VQDGSCKMLV TTVGMRTQWG KLMATLSEGG DDETPLQVKL
360 370 380 390 400
NGVATIIGKI GLFFAVITFA VLVQGLANQK RLDNSHWIWT ADELMAMLEY
410 420 430 440 450
FAVAVTIVVV AVPEGLPLAV TLSLAFAMKK MMNDKALVRN LAACETMGSA
460 470 480 490 500
TTICSDKTGT LTTNHMTVVK ACICEQAKEV NGPDAAMKFA SGIPESAVKL
510 520 530 540 550
LLQSIFTNTG GEIVVGKGNK TEILGTPTET ALLEFGLSLG GDFQEVRQAS
560 570 580 590 600
NVVKVEPFNS TKKRMGVVIE LPERHFRAHC KGASEIVLDS CDKYINKDGE
610 620 630 640 650
VVPLDEKSTS HLKNIIEEFA SEALRTLCLA YFEIGDEFSL EAPIPSGGYT
660 670 680 690 700
CIGIVGIKDP VRPGVKESVA ICKSAGITVR MVTGDNLTTA KAIARECGIL
710 720 730 740 750
TDDGIAIEGP EFREKSDEEL LKLIPKLQVM ARSSPMDKHT LVRLLRTMFQ
760 770 780 790 800
EVVAVTGDGT NDAPALHEAD IGLAMGISGT EVAKESADVI ILDDNFSTIV
810 820 830 840 850
TVAKWGRSVY INIQKFVQFQ LTVNVVALIV NFLSACLTGN APLTAVQLLW
860 870 880 890 900
VNMIMDTLGA LALATEPPQD DLMKRSPVGR KGNFISNVMW RNILGQSLYQ
910 920 930 940 950
LVIIWCLQTK GKTMFGLDGP DSDLTLNTLI FNIFVFCQVF NEISSREMEK
960 970 980 990 1000
IDVFKGILKN YVFVAVLTCT VVFQVIIIEL LGTFADTTPL NLGQWLVSII
1010 1020
LGFLGMPVAA ALKMIPVGSH
Length:1,020
Mass (Da):111,261
Last modified:April 30, 2003 - v3
Checksum:iBDD81AF7EC4AED6E
GO

Sequence cautioni

The sequence AAF24958 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA49558 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti88P → S in AAD10211 (PubMed:8234257).Curated1
Sequence conflicti88P → S in BAA03090 (Ref. 2) Curated1
Sequence conflicti88P → S in CAA49559 (Ref. 2) Curated1
Sequence conflicti801T → I in AAD10211 (PubMed:8234257).Curated1
Sequence conflicti801T → I in AAD10212 (PubMed:8234257).Curated1
Sequence conflicti801T → I in BAA03090 (Ref. 2) Curated1
Sequence conflicti801T → I in CAA49559 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08468 mRNA Translation: AAD10211.1
L08469 Genomic DNA Translation: AAD10212.1
D13983 mRNA Translation: BAA03090.1 Sequence problems.
D13984 Genomic DNA Translation: BAA03091.1 Sequence problems.
X69940 Genomic DNA Translation: CAA49558.1 Sequence problems.
X69941 mRNA Translation: CAA49559.1 Sequence problems.
AC012375 Genomic DNA Translation: AAF24958.1 Sequence problems.
AC079280 Genomic DNA Translation: AAG50579.1
CP002684 Genomic DNA Translation: AEE30878.1
CP002684 Genomic DNA Translation: ANM59134.1
PIRiD86402
S71168
T51925
T51926
RefSeqiNP_001321522.1, NM_001332787.1 [Q37145-1]
NP_849716.1, NM_179385.2 [Q37145-1]
UniGeneiAt.21941

Genome annotation databases

EnsemblPlantsiAT1G27770.1; AT1G27770.1; AT1G27770 [Q37145-1]
AT1G27770.4; AT1G27770.4; AT1G27770 [Q37145-1]
GeneIDi839670
GrameneiAT1G27770.1; AT1G27770.1; AT1G27770 [Q37145-1]
AT1G27770.4; AT1G27770.4; AT1G27770 [Q37145-1]
KEGGiath:AT1G27770

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiACA1_ARATH
AccessioniPrimary (citable) accession number: Q37145
Secondary accession number(s): Q37146
, Q42571, Q42587, Q9SFY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: April 30, 2003
Last modified: April 25, 2018
This is version 168 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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