UniProtKB - Q37145 (ACA1_ARATH)
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Protein
Calcium-transporting ATPase 1
Gene
ACA1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles.
Catalytic activityi
ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).
Enzyme regulationi
Activated by calmodulin.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 456 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 758 | MagnesiumBy similarity | 1 | |
Metal bindingi | 762 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- calcium channel activity Source: TAIR
- calcium-transporting ATPase activity Source: TAIR
- calmodulin binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
Keywordsi
Molecular function | Calmodulin-binding, Hydrolase |
Biological process | Calcium transport, Ion transport, Transport |
Ligand | ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT1G27770-MONOMER. MetaCyc:MONOMER-14612. |
Reactomei | R-ATH-418359. Reduction of cytosolic Ca++ levels. R-ATH-5578775. Ion homeostasis. R-ATH-936837. Ion transport by P-type ATPases. |
Protein family/group databases
TCDBi | 3.A.3.2.11. the p-type atpase (p-atpase) superfamily. |
Names & Taxonomyi
Protein namesi | Recommended name: Calcium-transporting ATPase 1 (EC:3.6.3.8)Alternative name(s): Ca(2+)-ATPase isoform 1 Plastid envelope ATPase 1 |
Gene namesi | Name:ACA1 Synonyms:PEA1 Ordered Locus Names:At1g27770 ORF Names:F28L5.1, T22C5.23 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G27770. |
TAIRi | locus:2029794. AT1G27770. |
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 162 | StromalSequence analysisAdd BLAST | 162 | |
Transmembranei | 163 – 183 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 184 – 201 | LumenalSequence analysisAdd BLAST | 18 | |
Transmembranei | 202 – 222 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 223 – 350 | StromalSequence analysisAdd BLAST | 128 | |
Transmembranei | 351 – 370 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 371 – 400 | LumenalSequence analysisAdd BLAST | 30 | |
Transmembranei | 401 – 418 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 419 – 813 | StromalSequence analysisAdd BLAST | 395 | |
Transmembranei | 814 – 832 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 833 – 843 | LumenalSequence analysisAdd BLAST | 11 | |
Transmembranei | 844 – 864 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 865 – 884 | StromalSequence analysisAdd BLAST | 20 | |
Transmembranei | 885 – 907 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 908 – 919 | LumenalSequence analysisAdd BLAST | 12 | |
Transmembranei | 920 – 941 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 942 – 959 | StromalSequence analysisAdd BLAST | 18 | |
Transmembranei | 960 – 981 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 982 – 991 | LumenalSequence analysis | 10 | |
Transmembranei | 992 – 1013 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 1014 – 1020 | StromalSequence analysis | 7 |
Keywords - Cellular componenti
Chloroplast, Membrane, Plastid, Plastid inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046410 | 1 – 1020 | Calcium-transporting ATPase 1Add BLAST | 1020 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 46 | Phosphoserine; by CPKBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q37145. |
PTM databases
iPTMneti | Q37145. |
SwissPalmi | Q37145. |
Expressioni
Tissue specificityi
Expressed at higher levels in roots than in leaves.
Gene expression databases
ExpressionAtlasi | Q37145. baseline and differential. |
Genevisiblei | Q37145. AT. |
Interactioni
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
Protein-protein interaction databases
STRINGi | 3702.AT1G27770.1. |
Structurei
3D structure databases
ProteinModelPortali | Q37145. |
SMRi | Q37145. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 21 – 32 | Interaction with calmodulinBy similarityAdd BLAST | 12 |
Domaini
The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.By similarity
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0204. Eukaryota. ENOG410XNNC. LUCA. |
HOGENOMi | HOG000265623. |
InParanoidi | Q37145. |
KOi | K01537. |
OMAi | NAVDCNQ. |
OrthoDBi | EOG093600UZ. |
PhylomeDBi | Q37145. |
Family and domain databases
Gene3Di | 3.40.1110.10. 1 hit. 3.40.50.1000. 2 hits. |
InterProi | View protein in InterPro IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR023299. ATPase_P-typ_cyto_dom_N. IPR018303. ATPase_P-typ_P_site. IPR023298. ATPase_P-typ_TM_dom_sf. IPR008250. ATPase_P-typ_transduc_dom_A_sf. IPR024750. Ca_ATPase_N_dom. IPR036412. HAD-like_sf. IPR023214. HAD_sf. IPR006408. P-type_ATPase_IIB. IPR001757. P_typ_ATPase. |
Pfami | View protein in Pfam PF12515. CaATP_NAI. 1 hit. PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. |
PRINTSi | PR00120. HATPASE. |
SMARTi | View protein in SMART SM00831. Cation_ATPase_N. 1 hit. |
SUPFAMi | SSF56784. SSF56784. 2 hits. SSF81653. SSF81653. 1 hit. SSF81660. SSF81660. 1 hit. SSF81665. SSF81665. 3 hits. |
TIGRFAMsi | TIGR01517. ATPase-IIB_Ca. 1 hit. TIGR01494. ATPase_P-type. 2 hits. |
PROSITEi | View protein in PROSITE PS00154. ATPASE_E1_E2. 1 hit. |
i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basket
Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q37145-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MESYLNENFG DVKPKNSSDE ALQRWRKLCW IVKNPKRRFR FTANLSKRSE
60 70 80 90 100
AEAIRRSNQE KFRVAVLVSQ AALQFINSLK LSSEYTLPEE VRKAGFEICP
110 120 130 140 150
DELGSIVEGH DLKKLKIHGG TEGLTEKLST SIASGISTSE DLLSVRKEIY
160 170 180 190 200
GINQFTESPS RGFWLFVWEA LQDTTLMILA ACAFVSLIVG ILMEGWPIGA
210 220 230 240 250
HDGLGIVASI LLVVFVTATS DYRQSLQFKD LDAEKKKIVV QVTRDKLRQK
260 270 280 290 300
ISIYDLLPGD VVHLGIGDQI PADGLFISGF SVLINESSLT GESEPVSVSV
310 320 330 340 350
EHPFLLSGTK VQDGSCKMLV TTVGMRTQWG KLMATLSEGG DDETPLQVKL
360 370 380 390 400
NGVATIIGKI GLFFAVITFA VLVQGLANQK RLDNSHWIWT ADELMAMLEY
410 420 430 440 450
FAVAVTIVVV AVPEGLPLAV TLSLAFAMKK MMNDKALVRN LAACETMGSA
460 470 480 490 500
TTICSDKTGT LTTNHMTVVK ACICEQAKEV NGPDAAMKFA SGIPESAVKL
510 520 530 540 550
LLQSIFTNTG GEIVVGKGNK TEILGTPTET ALLEFGLSLG GDFQEVRQAS
560 570 580 590 600
NVVKVEPFNS TKKRMGVVIE LPERHFRAHC KGASEIVLDS CDKYINKDGE
610 620 630 640 650
VVPLDEKSTS HLKNIIEEFA SEALRTLCLA YFEIGDEFSL EAPIPSGGYT
660 670 680 690 700
CIGIVGIKDP VRPGVKESVA ICKSAGITVR MVTGDNLTTA KAIARECGIL
710 720 730 740 750
TDDGIAIEGP EFREKSDEEL LKLIPKLQVM ARSSPMDKHT LVRLLRTMFQ
760 770 780 790 800
EVVAVTGDGT NDAPALHEAD IGLAMGISGT EVAKESADVI ILDDNFSTIV
810 820 830 840 850
TVAKWGRSVY INIQKFVQFQ LTVNVVALIV NFLSACLTGN APLTAVQLLW
860 870 880 890 900
VNMIMDTLGA LALATEPPQD DLMKRSPVGR KGNFISNVMW RNILGQSLYQ
910 920 930 940 950
LVIIWCLQTK GKTMFGLDGP DSDLTLNTLI FNIFVFCQVF NEISSREMEK
960 970 980 990 1000
IDVFKGILKN YVFVAVLTCT VVFQVIIIEL LGTFADTTPL NLGQWLVSII
1010 1020
LGFLGMPVAA ALKMIPVGSH
Sequence cautioni
The sequence AAF24958 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA49558 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 88 | P → S in AAD10211 (PubMed:8234257).Curated | 1 | |
Sequence conflicti | 88 | P → S in BAA03090 (Ref. 2) Curated | 1 | |
Sequence conflicti | 88 | P → S in CAA49559 (Ref. 2) Curated | 1 | |
Sequence conflicti | 801 | T → I in AAD10211 (PubMed:8234257).Curated | 1 | |
Sequence conflicti | 801 | T → I in AAD10212 (PubMed:8234257).Curated | 1 | |
Sequence conflicti | 801 | T → I in BAA03090 (Ref. 2) Curated | 1 | |
Sequence conflicti | 801 | T → I in CAA49559 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L08468 mRNA. Translation: AAD10211.1. L08469 Genomic DNA. Translation: AAD10212.1. D13983 mRNA. Translation: BAA03090.1. Sequence problems. D13984 Genomic DNA. Translation: BAA03091.1. Sequence problems. X69940 Genomic DNA. Translation: CAA49558.1. Sequence problems. X69941 mRNA. Translation: CAA49559.1. Sequence problems. AC012375 Genomic DNA. Translation: AAF24958.1. Sequence problems. AC079280 Genomic DNA. Translation: AAG50579.1. CP002684 Genomic DNA. Translation: AEE30878.1. CP002684 Genomic DNA. Translation: ANM59134.1. |
PIRi | D86402. S71168. T51925. T51926. |
RefSeqi | NP_001321522.1. NM_001332787.1. [Q37145-1] NP_849716.1. NM_179385.2. [Q37145-1] |
UniGenei | At.21941. |
Genome annotation databases
EnsemblPlantsi | AT1G27770.1; AT1G27770.1; AT1G27770. [Q37145-1] AT1G27770.4; AT1G27770.4; AT1G27770. [Q37145-1] |
GeneIDi | 839670. |
Gramenei | AT1G27770.1; AT1G27770.1; AT1G27770. [Q37145-1] AT1G27770.4; AT1G27770.4; AT1G27770. [Q37145-1] |
KEGGi | ath:AT1G27770. |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Entry informationi
Entry namei | ACA1_ARATH | |
Accessioni | Q37145Primary (citable) accession number: Q37145 Secondary accession number(s): Q37146 Q9SFY1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | April 30, 2003 | |
Last modified: | February 28, 2018 | |
This is version 167 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |