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Reviewed, UniProtKB/Swiss-Prot Q37145 (ACA1_ARATH)

Last modified November 3, 2009. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Calcium-transporting ATPase 1, chloroplastic
    EC=3.6.3.8
Alternative name(s):
    Ca(2+)-ATPase isoform 1
    Plastid envelope ATPase 1
Gene names
Name: ACA1
Synonyms: PEA1
Ordered Locus Names: At1g27770
ORF Names: T22C5.23, F28L5.1
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length1020 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles.

Catalytic activity

ATP + H2O + Ca2+(Cis) = ADP + phosphate + Ca2+(Trans).

Enzyme regulation

Activated by calmodulin By similarity.

Subcellular location

Plastid › chloroplast inner membrane; Multi-pass membrane protein Probable.

Tissue specificity

Expressed at higher levels in roots than in leaves.

Domain

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion By similarity.

Sequence similarities

Belongs to the cation transport ATPase (P-type) family. Type IIB subfamily.

Sequence caution

The sequence AAF24958.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAA49558.1 differs from that shown. Reason: Erroneous gene model prediction.

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Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q37145-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 1020Calcium-transporting ATPase 1, chloroplasticPRO_0000046410

Regions

Topological domain1 – 162162Stromal Potential
Transmembrane163 – 18321 Potential
Topological domain184 – 20118Lumenal Potential
Transmembrane202 – 22221 Potential
Topological domain223 – 350128Stromal Potential
Transmembrane351 – 37020 Potential
Topological domain371 – 40030Lumenal Potential
Transmembrane401 – 41818 Potential
Topological domain419 – 813395Stromal Potential
Transmembrane814 – 83219 Potential
Topological domain833 – 84311Lumenal Potential
Transmembrane844 – 86421 Potential
Topological domain865 – 88420Stromal Potential
Transmembrane885 – 90723 Potential
Topological domain908 – 91912Lumenal Potential
Transmembrane920 – 94122 Potential
Topological domain942 – 95918Stromal Potential
Transmembrane960 – 98122 Potential
Topological domain982 – 99110Lumenal Potential
Transmembrane992 – 101322 Potential
Topological domain1014 – 10207Stromal Potential
Region21 – 3212Interaction with calmodulin By similarity

Sites

Active site45614-aspartylphosphate intermediate By similarity
Metal binding7581Magnesium By similarity
Metal binding7621Magnesium By similarity

Amino acid modifications

Modified residue461Phosphoserine; by CPK By similarity

Experimental info

Sequence conflict881P → S in AAD10211. Ref.1
Sequence conflict881P → S in BAA03090. Ref.2
Sequence conflict881P → S in CAA49559. Ref.2
Sequence conflict8011T → I in AAD10211. Ref.1
Sequence conflict8011T → I in AAD10212. Ref.1
Sequence conflict8011T → I in BAA03090. Ref.2
Sequence conflict8011T → I in CAA49559. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 30, 2003. Version 3.
Checksum: BDD81AF7EC4AED6E

FASTA1,020111,261
        10         20         30         40         50         60 
MESYLNENFG DVKPKNSSDE ALQRWRKLCW IVKNPKRRFR FTANLSKRSE AEAIRRSNQE 

        70         80         90        100        110        120 
KFRVAVLVSQ AALQFINSLK LSSEYTLPEE VRKAGFEICP DELGSIVEGH DLKKLKIHGG 

       130        140        150        160        170        180 
TEGLTEKLST SIASGISTSE DLLSVRKEIY GINQFTESPS RGFWLFVWEA LQDTTLMILA 

       190        200        210        220        230        240 
ACAFVSLIVG ILMEGWPIGA HDGLGIVASI LLVVFVTATS DYRQSLQFKD LDAEKKKIVV 

       250        260        270        280        290        300 
QVTRDKLRQK ISIYDLLPGD VVHLGIGDQI PADGLFISGF SVLINESSLT GESEPVSVSV 

       310        320        330        340        350        360 
EHPFLLSGTK VQDGSCKMLV TTVGMRTQWG KLMATLSEGG DDETPLQVKL NGVATIIGKI 

       370        380        390        400        410        420 
GLFFAVITFA VLVQGLANQK RLDNSHWIWT ADELMAMLEY FAVAVTIVVV AVPEGLPLAV 

       430        440        450        460        470        480 
TLSLAFAMKK MMNDKALVRN LAACETMGSA TTICSDKTGT LTTNHMTVVK ACICEQAKEV 

       490        500        510        520        530        540 
NGPDAAMKFA SGIPESAVKL LLQSIFTNTG GEIVVGKGNK TEILGTPTET ALLEFGLSLG 

       550        560        570        580        590        600 
GDFQEVRQAS NVVKVEPFNS TKKRMGVVIE LPERHFRAHC KGASEIVLDS CDKYINKDGE 

       610        620        630        640        650        660 
VVPLDEKSTS HLKNIIEEFA SEALRTLCLA YFEIGDEFSL EAPIPSGGYT CIGIVGIKDP 

       670        680        690        700        710        720 
VRPGVKESVA ICKSAGITVR MVTGDNLTTA KAIARECGIL TDDGIAIEGP EFREKSDEEL 

       730        740        750        760        770        780 
LKLIPKLQVM ARSSPMDKHT LVRLLRTMFQ EVVAVTGDGT NDAPALHEAD IGLAMGISGT 

       790        800        810        820        830        840 
EVAKESADVI ILDDNFSTIV TVAKWGRSVY INIQKFVQFQ LTVNVVALIV NFLSACLTGN 

       850        860        870        880        890        900 
APLTAVQLLW VNMIMDTLGA LALATEPPQD DLMKRSPVGR KGNFISNVMW RNILGQSLYQ 

       910        920        930        940        950        960 
LVIIWCLQTK GKTMFGLDGP DSDLTLNTLI FNIFVFCQVF NEISSREMEK IDVFKGILKN 

       970        980        990       1000       1010       1020 
YVFVAVLTCT VVFQVIIIEL LGTFADTTPL NLGQWLVSII LGFLGMPVAA ALKMIPVGSH

« Hide

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References

« Hide 'large scale' references
[1]"Characterization of a gene encoding a Ca(2+)-ATPase-like protein in the plastid envelope."
Huang L., Berkelman T., Franklin A.E., Hoffman N.E.
Proc. Natl. Acad. Sci. U.S.A. 90:10066-10070(1993) [PubMed: 8234257] [Abstract]
Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: cv. Columbia.
[2]Erratum
Huang L., Berkelman T., Franklin A.E., Hoffman N.E.
Proc. Natl. Acad. Sci. U.S.A. 91:9664-9664(1994)
Cited for: SEQUENCE REVISION.
[3]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

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Cross-references

Sequence databases

L08468 mRNA. Translation: AAD10211.1.
L08469 Genomic DNA. Translation: AAD10212.1.
D13983 mRNA. Translation: BAA03090.1. Sequence problems.
D13984 Genomic DNA. Translation: BAA03091.1. Sequence problems.
X69940 Genomic DNA. Translation: CAA49558.1. Sequence problems.
X69941 mRNA. Translation: CAA49559.1. Sequence problems.
AC012375 Genomic DNA. Translation: AAF24958.1. Sequence problems.
AC079280 Genomic DNA. Translation: AAG50579.1.
IPIIPI00533944.
PIRD86402.
S71168.
T51925.
T51926.
RefSeqNP_849716.1.
UniGeneAt.21941

3D structure databases

HSSPHSSP built from PDB template 1EUL based on UniProtKB P04191.
ModBaseSearch...

Protein family/group databases

TCDB3.A.3.2.11. P-type ATPase (P-ATPase) superfamily.

Proteomic databases

PRIDEQ37145.

Genome annotation databases

GeneID839670.
GenomeReviewsGene locus AT1G27770 in contig CT485782_GR.
NMPDRfig|3702.1.peg.3111.

Organism-specific databases

TAIRAt1g27770.

Phylogenomic databases

OMAHINGDYN.

Enzyme and pathway databases

BRENDA3.6.3.8. 302.

Gene expression databases

ArrayExpressQ37145.
GenevestigatorQ37145.

Family and domain databases

InterProIPR008250. ATPase_P-typ_ATPase-assoc-reg.
IPR006408. ATPase_P-typ_Ca-transp_PMCA.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR000695. ATPase_P-typ_H-transp.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR005834. Dehalogen-like_hydro.
[Graphical view]
PANTHERPTHR11939. ATPase_P. 1 hit.
PfamPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00120. HATPASE.
TIGRFAMsTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameACA1_ARATH
AccessionPrimary (citable) accession number: Q37145
Secondary accession number(s): Q37146 expand/collapse secondary AC list , Q42571, Q42587, Q9SFY1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: April 30, 2003
Last modified: November 3, 2009
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents