Calcium-transporting ATPase 1, chloroplastic precursor - Arabidopsis thaliana (Mouse-ear cress)

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Reviewed, UniProtKB/Swiss-Prot Q37145 (ACA1_ARATH)

Last modified November 24, 2009. Version 95. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Calcium-transporting ATPase 1, chloroplastic
    EC=3.6.3.8
Alternative name(s):
    Ca(2+)-ATPase isoform 1
    Plastid envelope ATPase 1

Gene names

Name:	ACA1
Synonyms:	PEA1
Ordered Locus Names:	At1g27770
ORF Names:	T22C5.23, F28L5.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	1020 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles.
Catalytic activity	ATP + H₂O + Ca²⁺(Cis) = ADP + phosphate + Ca²⁺(Trans).
Enzyme regulation	Activated by calmodulin By similarity.
Subcellular location	Plastid › chloroplast inner membrane; Multi-pass membrane protein Probable.
Tissue specificity	Expressed at higher levels in roots than in leaves.
Domain	The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion By similarity.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IIB subfamily.
Sequence caution	The sequence AAF24958.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA49558.1 differs from that shown. Reason: Erroneous gene model prediction.

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Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Chloroplast Membrane Plastid Plastid inner membrane
Coding sequence diversity	Alternative splicing
Domain	Transit peptide Transmembrane
Ligand	ATP-binding Calcium Calmodulin-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro calcium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast inner membrane Ref.1 Inferred from direct assay. Source: TAIR endoplasmic reticulum Inferred from direct assay. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium channel activity Traceable author statement. Source: TAIR calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-transporting ATPase activity Traceable author statement. Source: TAIR calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q37145-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Chloroplast Potential

Chain

? – 1020

Calcium-transporting ATPase 1, chloroplastic

PRO_0000046410

Regions

Topological domain

1 – 162

162

Stromal Potential

Transmembrane

163 – 183

Potential

Topological domain

184 – 201

Lumenal Potential

Transmembrane

202 – 222

Potential

Topological domain

223 – 350

128

Stromal Potential

Transmembrane

351 – 370

Potential

Topological domain

371 – 400

Lumenal Potential

Transmembrane

401 – 418

Potential

Topological domain

419 – 813

395

Stromal Potential

Transmembrane

814 – 832

Potential

Topological domain

833 – 843

Lumenal Potential

Transmembrane

844 – 864

Potential

Topological domain

865 – 884

Stromal Potential

Transmembrane

885 – 907

Potential

Topological domain

908 – 919

Lumenal Potential

Transmembrane

920 – 941

Potential

Topological domain

942 – 959

Stromal Potential

Transmembrane

960 – 981

Potential

Topological domain

982 – 991

Lumenal Potential

Transmembrane

992 – 1013

Potential

Topological domain

1014 – 1020

Stromal Potential

Region

21 – 32

Interaction with calmodulin By similarity

Sites

Active site

456

4-aspartylphosphate intermediate By similarity

Metal binding

758

Magnesium By similarity

Metal binding

762

Magnesium By similarity

Amino acid modifications

Modified residue

Phosphoserine; by CPK By similarity

Experimental info

Sequence conflict

P → S in AAD10211. Ref.1

Sequence conflict

P → S in BAA03090. Ref.2

Sequence conflict

P → S in CAA49559. Ref.2

Sequence conflict

801

T → I in AAD10211. Ref.1

Sequence conflict

801

T → I in AAD10212. Ref.1

Sequence conflict

801

T → I in BAA03090. Ref.2

Sequence conflict

801

T → I in CAA49559. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified April 30, 2003. Version 3.
Checksum: BDD81AF7EC4AED6E
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FASTA

1,020

111,261

        10         20         30         40         50         60 
MESYLNENFG DVKPKNSSDE ALQRWRKLCW IVKNPKRRFR FTANLSKRSE AEAIRRSNQE 

        70         80         90        100        110        120 
KFRVAVLVSQ AALQFINSLK LSSEYTLPEE VRKAGFEICP DELGSIVEGH DLKKLKIHGG 

       130        140        150        160        170        180 
TEGLTEKLST SIASGISTSE DLLSVRKEIY GINQFTESPS RGFWLFVWEA LQDTTLMILA 

       190        200        210        220        230        240 
ACAFVSLIVG ILMEGWPIGA HDGLGIVASI LLVVFVTATS DYRQSLQFKD LDAEKKKIVV 

       250        260        270        280        290        300 
QVTRDKLRQK ISIYDLLPGD VVHLGIGDQI PADGLFISGF SVLINESSLT GESEPVSVSV 

       310        320        330        340        350        360 
EHPFLLSGTK VQDGSCKMLV TTVGMRTQWG KLMATLSEGG DDETPLQVKL NGVATIIGKI 

       370        380        390        400        410        420 
GLFFAVITFA VLVQGLANQK RLDNSHWIWT ADELMAMLEY FAVAVTIVVV AVPEGLPLAV 

       430        440        450        460        470        480 
TLSLAFAMKK MMNDKALVRN LAACETMGSA TTICSDKTGT LTTNHMTVVK ACICEQAKEV 

       490        500        510        520        530        540 
NGPDAAMKFA SGIPESAVKL LLQSIFTNTG GEIVVGKGNK TEILGTPTET ALLEFGLSLG 

       550        560        570        580        590        600 
GDFQEVRQAS NVVKVEPFNS TKKRMGVVIE LPERHFRAHC KGASEIVLDS CDKYINKDGE 

       610        620        630        640        650        660 
VVPLDEKSTS HLKNIIEEFA SEALRTLCLA YFEIGDEFSL EAPIPSGGYT CIGIVGIKDP 

       670        680        690        700        710        720 
VRPGVKESVA ICKSAGITVR MVTGDNLTTA KAIARECGIL TDDGIAIEGP EFREKSDEEL 

       730        740        750        760        770        780 
LKLIPKLQVM ARSSPMDKHT LVRLLRTMFQ EVVAVTGDGT NDAPALHEAD IGLAMGISGT 

       790        800        810        820        830        840 
EVAKESADVI ILDDNFSTIV TVAKWGRSVY INIQKFVQFQ LTVNVVALIV NFLSACLTGN 

       850        860        870        880        890        900 
APLTAVQLLW VNMIMDTLGA LALATEPPQD DLMKRSPVGR KGNFISNVMW RNILGQSLYQ 

       910        920        930        940        950        960 
LVIIWCLQTK GKTMFGLDGP DSDLTLNTLI FNIFVFCQVF NEISSREMEK IDVFKGILKN 

       970        980        990       1000       1010       1020 
YVFVAVLTCT VVFQVIIIEL LGTFADTTPL NLGQWLVSII LGFLGMPVAA ALKMIPVGSH

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a gene encoding a Ca(2+)-ATPase-like protein in the plastid envelope." Huang L., Berkelman T., Franklin A.E., Hoffman N.E. Proc. Natl. Acad. Sci. U.S.A. 90:10066-10070(1993) [PubMed: 8234257] [Abstract] Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Columbia.
[2]	Erratum Huang L., Berkelman T., Franklin A.E., Hoffman N.E. Proc. Natl. Acad. Sci. U.S.A. 91:9664-9664(1994) Cited for: SEQUENCE REVISION.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	L08468 mRNA. Translation: AAD10211.1. L08469 Genomic DNA. Translation: AAD10212.1. D13983 mRNA. Translation: BAA03090.1. Sequence problems. D13984 Genomic DNA. Translation: BAA03091.1. Sequence problems. X69940 Genomic DNA. Translation: CAA49558.1. Sequence problems. X69941 mRNA. Translation: CAA49559.1. Sequence problems. AC012375 Genomic DNA. Translation: AAF24958.1. Sequence problems. AC079280 Genomic DNA. Translation: AAG50579.1.
IPI	IPI00533944.
PIR	D86402. S71168. T51925. T51926.
RefSeq	NP_849716.1.
UniGene	At.21941
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.A.3.2.11. P-type ATPase (P-ATPase) superfamily.
Proteomic databases
PRIDE	Q37145.
Genome annotation databases
GeneID	839670.
GenomeReviews	Gene locus AT1G27770 in contig CT485782_GR.
NMPDR	fig\|3702.1.peg.3111.
Organism-specific databases
TAIR	At1g27770.
Phylogenomic databases
OMA	FRAHCKG
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14612.
BRENDA	3.6.3.8. 302.
Gene expression databases
ArrayExpress	Q37145.
Genevestigator	Q37145.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-reg. IPR006408. ATPase_P-typ_Ca-transp_PMCA. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR000695. ATPase_P-typ_H-transp. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00120. HATPASE.
SMART	SM00831. Cation_ATPase_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01517. ATPase-IIB_Ca. 1 hit. TIGR01494. ATPase_P-type. 3 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

ACA1_ARATH

Accession

Primary (citable) accession number: Q37145
Secondary accession number(s): Q37146

Q9SFY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	April 30, 2003
Last modified:	November 24, 2009
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents