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Protein

Calcium-transporting ATPase 1, chloroplastic

Gene

ACA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulationi

Activated by calmodulin.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei456 – 45614-aspartylphosphate intermediateBy similarity
Metal bindingi758 – 7581MagnesiumBy similarity
Metal bindingi762 – 7621MagnesiumBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium channel activity Source: TAIR
  • calcium-transporting ATPase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G27770-MONOMER.
ARA:GQT-1178-MONOMER.
MetaCyc:MONOMER-14612.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Protein family/group databases

TCDBi3.A.3.2.11. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 1, chloroplastic (EC:3.6.3.8)
Alternative name(s):
Ca(2+)-ATPase isoform 1
Plastid envelope ATPase 1
Gene namesi
Name:ACA1
Synonyms:PEA1
Ordered Locus Names:At1g27770
ORF Names:F28L5.1, T22C5.23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G27770.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 162162StromalSequence analysisAdd
BLAST
Transmembranei163 – 18321HelicalSequence analysisAdd
BLAST
Topological domaini184 – 20118LumenalSequence analysisAdd
BLAST
Transmembranei202 – 22221HelicalSequence analysisAdd
BLAST
Topological domaini223 – 350128StromalSequence analysisAdd
BLAST
Transmembranei351 – 37020HelicalSequence analysisAdd
BLAST
Topological domaini371 – 40030LumenalSequence analysisAdd
BLAST
Transmembranei401 – 41818HelicalSequence analysisAdd
BLAST
Topological domaini419 – 813395StromalSequence analysisAdd
BLAST
Transmembranei814 – 83219HelicalSequence analysisAdd
BLAST
Topological domaini833 – 84311LumenalSequence analysisAdd
BLAST
Transmembranei844 – 86421HelicalSequence analysisAdd
BLAST
Topological domaini865 – 88420StromalSequence analysisAdd
BLAST
Transmembranei885 – 90723HelicalSequence analysisAdd
BLAST
Topological domaini908 – 91912LumenalSequence analysisAdd
BLAST
Transmembranei920 – 94122HelicalSequence analysisAdd
BLAST
Topological domaini942 – 95918StromalSequence analysisAdd
BLAST
Transmembranei960 – 98122HelicalSequence analysisAdd
BLAST
Topological domaini982 – 99110LumenalSequence analysis
Transmembranei992 – 101322HelicalSequence analysisAdd
BLAST
Topological domaini1014 – 10207StromalSequence analysis

GO - Cellular componenti

  • chloroplast inner membrane Source: TAIR
  • endoplasmic reticulum Source: TAIR
  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Plastid inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 1020Calcium-transporting ATPase 1, chloroplasticPRO_0000046410
Transit peptidei1 – ?ChloroplastSequence analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei46 – 461Phosphoserine; by CPKBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ37145.
PRIDEiQ37145.

PTM databases

iPTMnetiQ37145.
SwissPalmiQ37145.

Expressioni

Tissue specificityi

Expressed at higher levels in roots than in leaves.

Gene expression databases

ExpressionAtlasiQ37145. baseline and differential.
GenevisibleiQ37145. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G27770.1.

Structurei

3D structure databases

ProteinModelPortaliQ37145.
SMRiQ37145. Positions 148-886.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 3212Interaction with calmodulinBy similarityAdd
BLAST

Domaini

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
HOGENOMiHOG000265623.
InParanoidiQ37145.
KOiK01537.
OMAiFEICPDE.
PhylomeDBiQ37145.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR024750. Ca_ATPase_N_dom.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF12515. CaATP_NAI. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q37145-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESYLNENFG DVKPKNSSDE ALQRWRKLCW IVKNPKRRFR FTANLSKRSE
60 70 80 90 100
AEAIRRSNQE KFRVAVLVSQ AALQFINSLK LSSEYTLPEE VRKAGFEICP
110 120 130 140 150
DELGSIVEGH DLKKLKIHGG TEGLTEKLST SIASGISTSE DLLSVRKEIY
160 170 180 190 200
GINQFTESPS RGFWLFVWEA LQDTTLMILA ACAFVSLIVG ILMEGWPIGA
210 220 230 240 250
HDGLGIVASI LLVVFVTATS DYRQSLQFKD LDAEKKKIVV QVTRDKLRQK
260 270 280 290 300
ISIYDLLPGD VVHLGIGDQI PADGLFISGF SVLINESSLT GESEPVSVSV
310 320 330 340 350
EHPFLLSGTK VQDGSCKMLV TTVGMRTQWG KLMATLSEGG DDETPLQVKL
360 370 380 390 400
NGVATIIGKI GLFFAVITFA VLVQGLANQK RLDNSHWIWT ADELMAMLEY
410 420 430 440 450
FAVAVTIVVV AVPEGLPLAV TLSLAFAMKK MMNDKALVRN LAACETMGSA
460 470 480 490 500
TTICSDKTGT LTTNHMTVVK ACICEQAKEV NGPDAAMKFA SGIPESAVKL
510 520 530 540 550
LLQSIFTNTG GEIVVGKGNK TEILGTPTET ALLEFGLSLG GDFQEVRQAS
560 570 580 590 600
NVVKVEPFNS TKKRMGVVIE LPERHFRAHC KGASEIVLDS CDKYINKDGE
610 620 630 640 650
VVPLDEKSTS HLKNIIEEFA SEALRTLCLA YFEIGDEFSL EAPIPSGGYT
660 670 680 690 700
CIGIVGIKDP VRPGVKESVA ICKSAGITVR MVTGDNLTTA KAIARECGIL
710 720 730 740 750
TDDGIAIEGP EFREKSDEEL LKLIPKLQVM ARSSPMDKHT LVRLLRTMFQ
760 770 780 790 800
EVVAVTGDGT NDAPALHEAD IGLAMGISGT EVAKESADVI ILDDNFSTIV
810 820 830 840 850
TVAKWGRSVY INIQKFVQFQ LTVNVVALIV NFLSACLTGN APLTAVQLLW
860 870 880 890 900
VNMIMDTLGA LALATEPPQD DLMKRSPVGR KGNFISNVMW RNILGQSLYQ
910 920 930 940 950
LVIIWCLQTK GKTMFGLDGP DSDLTLNTLI FNIFVFCQVF NEISSREMEK
960 970 980 990 1000
IDVFKGILKN YVFVAVLTCT VVFQVIIIEL LGTFADTTPL NLGQWLVSII
1010 1020
LGFLGMPVAA ALKMIPVGSH
Length:1,020
Mass (Da):111,261
Last modified:April 30, 2003 - v3
Checksum:iBDD81AF7EC4AED6E
GO

Sequence cautioni

The sequence AAF24958.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAA49558.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti88 – 881P → S in AAD10211 (PubMed:8234257).Curated
Sequence conflicti88 – 881P → S in BAA03090 (Ref. 2) Curated
Sequence conflicti88 – 881P → S in CAA49559 (Ref. 2) Curated
Sequence conflicti801 – 8011T → I in AAD10211 (PubMed:8234257).Curated
Sequence conflicti801 – 8011T → I in AAD10212 (PubMed:8234257).Curated
Sequence conflicti801 – 8011T → I in BAA03090 (Ref. 2) Curated
Sequence conflicti801 – 8011T → I in CAA49559 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08468 mRNA. Translation: AAD10211.1.
L08469 Genomic DNA. Translation: AAD10212.1.
D13983 mRNA. Translation: BAA03090.1. Sequence problems.
D13984 Genomic DNA. Translation: BAA03091.1. Sequence problems.
X69940 Genomic DNA. Translation: CAA49558.1. Sequence problems.
X69941 mRNA. Translation: CAA49559.1. Sequence problems.
AC012375 Genomic DNA. Translation: AAF24958.1. Sequence problems.
AC079280 Genomic DNA. Translation: AAG50579.1.
CP002684 Genomic DNA. Translation: AEE30878.1.
PIRiD86402.
S71168.
T51925.
T51926.
RefSeqiNP_849716.1. NM_179385.1. [Q37145-1]
UniGeneiAt.21941.

Genome annotation databases

EnsemblPlantsiAT1G27770.1; AT1G27770.1; AT1G27770. [Q37145-1]
GeneIDi839670.
KEGGiath:AT1G27770.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08468 mRNA. Translation: AAD10211.1.
L08469 Genomic DNA. Translation: AAD10212.1.
D13983 mRNA. Translation: BAA03090.1. Sequence problems.
D13984 Genomic DNA. Translation: BAA03091.1. Sequence problems.
X69940 Genomic DNA. Translation: CAA49558.1. Sequence problems.
X69941 mRNA. Translation: CAA49559.1. Sequence problems.
AC012375 Genomic DNA. Translation: AAF24958.1. Sequence problems.
AC079280 Genomic DNA. Translation: AAG50579.1.
CP002684 Genomic DNA. Translation: AEE30878.1.
PIRiD86402.
S71168.
T51925.
T51926.
RefSeqiNP_849716.1. NM_179385.1. [Q37145-1]
UniGeneiAt.21941.

3D structure databases

ProteinModelPortaliQ37145.
SMRiQ37145. Positions 148-886.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G27770.1.

Protein family/group databases

TCDBi3.A.3.2.11. the p-type atpase (p-atpase) superfamily.

PTM databases

iPTMnetiQ37145.
SwissPalmiQ37145.

Proteomic databases

PaxDbiQ37145.
PRIDEiQ37145.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G27770.1; AT1G27770.1; AT1G27770. [Q37145-1]
GeneIDi839670.
KEGGiath:AT1G27770.

Organism-specific databases

TAIRiAT1G27770.

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
HOGENOMiHOG000265623.
InParanoidiQ37145.
KOiK01537.
OMAiFEICPDE.
PhylomeDBiQ37145.

Enzyme and pathway databases

BioCyciARA:AT1G27770-MONOMER.
ARA:GQT-1178-MONOMER.
MetaCyc:MONOMER-14612.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiQ37145.

Gene expression databases

ExpressionAtlasiQ37145. baseline and differential.
GenevisibleiQ37145. AT.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR024750. Ca_ATPase_N_dom.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF12515. CaATP_NAI. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a gene encoding a Ca(2+)-ATPase-like protein in the plastid envelope."
    Huang L., Berkelman T., Franklin A.E., Hoffman N.E.
    Proc. Natl. Acad. Sci. U.S.A. 90:10066-10070(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: cv. Columbia.
  2. Erratum
    Huang L., Berkelman T., Franklin A.E., Hoffman N.E.
    Proc. Natl. Acad. Sci. U.S.A. 91:9664-9664(1994)
    Cited for: SEQUENCE REVISION.
  3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiACA1_ARATH
AccessioniPrimary (citable) accession number: Q37145
Secondary accession number(s): Q37146
, Q42571, Q42587, Q9SFY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: April 30, 2003
Last modified: June 8, 2016
This is version 150 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.