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Protein

1-aminocyclopropane-1-carboxylate synthase 5

Gene

ACS5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene.

Catalytic activityi

S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + methylthioadenosine.1 Publication

Cofactori

Kineticsi

  1. KM=37 µM for S-adenosyl-L-methionine
  1. Vmax=81.70 µM/h/mg enzyme

pH dependencei

Optimum pH is 7.8.

Pathwayi: ethylene biosynthesis via S-adenosyl-L-methionine

This protein is involved in step 1 of the subpathway that synthesizes ethylene from S-adenosyl-L-methionine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 1-aminocyclopropane-1-carboxylate synthase 4 (ACS4), 1-aminocyclopropane-1-carboxylate synthase 8 (ACS8), 1-aminocyclopropane-1-carboxylate synthase 2 (ACS2), 1-aminocyclopropane-1-carboxylate synthase 7 (ACS7), 1-aminocyclopropane-1-carboxylate synthase 11 (ACS11), 1-aminocyclopropane-1-carboxylate synthase 9 (ACS9), 1-aminocyclopropane-1-carboxylate synthase 6 (ACS6), 1-aminocyclopropane-1-carboxylate synthase 5 (ACS5)
  2. 1-aminocyclopropane-1-carboxylate oxidase 2 (ACO2), 1-aminocyclopropane-1-carboxylate oxidase 3 (At1g12010), 1-aminocyclopropane-1-carboxylate oxidase 4 (ACO4), 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1), 1-aminocyclopropane-1-carboxylate oxidase 5 (At1g77330)
This subpathway is part of the pathway ethylene biosynthesis via S-adenosyl-L-methionine, which is itself part of Alkene biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ethylene from S-adenosyl-L-methionine, the pathway ethylene biosynthesis via S-adenosyl-L-methionine and in Alkene biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei47SubstrateBy similarity1
Binding sitei85SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cell division Source: TAIR
  • ethylene biosynthetic process Source: TAIR
  • fruit ripening Source: UniProtKB-KW
  • phloem or xylem histogenesis Source: TAIR
  • response to cytokinin Source: TAIR

Keywordsi

Molecular functionLyase
Biological processEthylene biosynthesis, Fruit ripening
LigandPyridoxal phosphate, S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi4.4.1.14 399
SABIO-RKiQ37001
UniPathwayiUPA00384; UER00562

Names & Taxonomyi

Protein namesi
Recommended name:
1-aminocyclopropane-1-carboxylate synthase 5 (EC:4.4.1.14)
Short name:
ACC synthase 5
Alternative name(s):
Ethylene-overproduction protein 2
S-adenosyl-L-methionine methylthioadenosine-lyase 5
Gene namesi
Name:ACS5
Synonyms:ACC5, ETO2
Ordered Locus Names:At5g65800
ORF Names:F6H11.90, MPA24.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G65800
TAIRilocus:2169980 AT5G65800

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi201S → F in cin5-3; defective in cytokinin induced ethylene. 1
Mutagenesisi269S → N in cin5-2; defective in cytokinin induced ethylene. 1
Mutagenesisi459 – 470RVSWT…VPDER → PGFMDRSCT in eto2; increases its stability leading to ethylene overproduction. Add BLAST12

Chemistry databases

ChEMBLiCHEMBL2242742

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001238991 – 4701-aminocyclopropane-1-carboxylate synthase 5Add BLAST470

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei272N6-(pyridoxal phosphate)lysineBy similarity1
Modified residuei461PhosphoserineBy similarity1

Post-translational modificationi

May be processed at its C-terminus.
Ubiquitinated (Probable). The interaction with ETO1 (and possibly EOL1 and EOL2) mediate its proteasome-dependent degradation. Its stability and degradation plays a central role in ethylene biosynthesis.Curated

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ37001

PTM databases

iPTMnetiQ37001

Expressioni

Tissue specificityi

Expressed in roots and siliques.1 Publication

Inductioni

By indole-3-acetic acid (IAA) and cycloheximide (CHX). In response to low dose of cytokinin.1 Publication

Gene expression databases

ExpressionAtlasiQ37001 differential
GenevisibleiQ37001 AT

Interactioni

Subunit structurei

Homodimer and heterodimer. In vivo, the relevance of heterodimerization with other ACS enzymes is however unsure (By similarity). Interacts (via its C-terminal region) with FEI1, FEI2, ETO1, EOL1 and EOL2 (PubMed:15118728, PubMed:19017745, PubMed:18808454). Interacts with GRF3 (PubMed:25122152).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ETO1O650203EBI-593450,EBI-593440

Protein-protein interaction databases

BioGridi21951, 8 interactors
IntActiQ37001, 3 interactors
STRINGi3702.AT5G65800.1

Structurei

3D structure databases

ProteinModelPortaliQ37001
SMRiQ37001
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0256 Eukaryota
COG0436 LUCA
HOGENOMiHOG000011234
InParanoidiQ37001
KOiK01762
OMAiANMSEGT
OrthoDBiEOG093608EZ
PhylomeDBiQ37001

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR004838 NHTrfase_class1_PyrdxlP-BS
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00105 AA_TRANSFER_CLASS_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q37001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQLSTKVTS NGHGQDSSYF LGWEEYEKNP YDEIKNPNGM IQMGLAENQL
60 70 80 90 100
CFDLIESWLT KNPDAASLKR NGQSIFRELA LFQDYHGMPE FKKAMAEFME
110 120 130 140 150
EIRGNRVTFD PKKIVLAAGS TSANETLMFC LAEPGDAFLL PTPYYPGFDR
160 170 180 190 200
DLKWRTGAEI VPIHCSSSNG FQITESALQQ AYQQAQKLDL KVKGVLVTNP
210 220 230 240 250
SNPLGTALTR RELNLLVDFI TSKNIHLISD EIYSGTMFGF EQFISVMDVL
260 270 280 290 300
KDKKLEDTEV SKRVHVVYSL SKDLGLPGFR VGAIYSNDEM IVSAATKMSS
310 320 330 340 350
FGLVSSQTQY LLSALLSDKK FTSQYLEENQ KRLKSRQRRL VSGLESAGIT
360 370 380 390 400
CLRSNAGLFC WVDMRHLLDT NTFEAELDLW KKIVYNVKLN ISPGSSCHCT
410 420 430 440 450
EPGWFRVCFA NMSEDTLDLA LKRLKTFVES TDCGRMISRS SHERLKSLRK
460 470
KTVSNWVFRV SWTDRVPDER
Length:470
Mass (Da):53,313
Last modified:November 1, 1996 - v1
Checksum:i8B2527C9B52AE19A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29261 mRNA Translation: AAA87292.1
L29260 Genomic DNA Translation: AAA87291.1
AB010075, AB020743 Genomic DNA Translation: BAB10687.1
AL021684 Genomic DNA Translation: CAA16680.1
CP002688 Genomic DNA Translation: AED98109.1
AF334720 mRNA Translation: AAG50098.1
AK229087 mRNA Translation: BAF00967.1
PIRiS71174
RefSeqiNP_201381.1, NM_125977.2
UniGeneiAt.1918

Genome annotation databases

EnsemblPlantsiAT5G65800.1; AT5G65800.1; AT5G65800
GeneIDi836709
GrameneiAT5G65800.1; AT5G65800.1; AT5G65800
KEGGiath:AT5G65800

Similar proteinsi

Entry informationi

Entry namei1A15_ARATH
AccessioniPrimary (citable) accession number: Q37001
Secondary accession number(s): O49537, Q0WPI2, Q9S9C3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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