Reviewed, UniProtKB/Swiss-Prot Q36863 (CLPP1_CYAPA)

Names and origin

Protein names

Recommended name:
    ATP-dependent Clp protease proteolytic subunit
    EC=3.4.21.92
Alternative name(s):
    Endopeptidase Clp

Gene names

Name:	clpP-A
Synonyms:	clpP1

Encoded on

Plastid; Cyanelle

Organism

Cyanophora paradoxa

Taxonomic identifier

2762 [NCBI]

Taxonomic lineage

Eukaryota › Glaucocystophyceae › Cyanophoraceae › Cyanophora

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subunit structure	Component of the chloroplastic Clp protease core complex. HAMAP MF_00444
Subcellular location	Plastid › cyanelle HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cyanelle Plastid
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cyanelle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	ATP-dependent Clp protease proteolytic subunit HAMAP MF_00444		PRO_0000179728
Sites
	Active site	98	1	By similarity
	Active site	123	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q36863-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7F519941355BF3A2	FASTA	194	21,816
	10 20 30 40 50 60 MTILIQQYTN DENKKLELNN ISKLLQNRII LFSQTVDDEI CNSIVGQLLY LENEDSTKDI 70 80 90 100 110 120 RLFINSPGGS VTAGLSVYDT IQNLSVDVST ICFGLAASMG AVLLAAGVEN KRFAFASSRI 130 140 150 160 170 180 MIHQPLSKVE APWSHLDIQI RNGAYFKNLL NNILSFHTKQ ELKQIETDTE RDFFLSATEA 190 KQYGIIDHIF INNN

References

[1]

"Nucleotide sequence of the cyanelle DNA from Cyanophora paradoxa." Stirewalt V.L., Michalowski C.B., Loeffelhardt W., Bohnert H.J., Bryant D.A. Plant Mol. Biol. Rep. 13:327-332(1995) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UTEX LB 555 / Pringsheim.

[2]

"The complete sequence of the cyanelle genome of Cyanophora paradoxa: the genetic complexity of a primitive plastid." Loeffelhardt W., Stirewalt V.L., Michalowski C.B., Annarella M., Farley J.Y., Schluchter W.M., Chung S., Newmann-Spallart C., Steiner J.M., Jakowitsch J., Bohnert H.J., Bryant D.A. (In) Schenk H.E.A., Herrmann R., Jeon K.W., Mueller N.E., Schwemmler W. (eds.); Eukaryotism and symbiosis, pp.40-48, Springer-Verlag, Heidelberg (1997) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UTEX LB 555 / Pringsheim.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U30821 Genomic DNA. Translation: AAA81174.1. U30821 Genomic DNA. Translation: AAA81292.1.
PIR	T06831.
RefSeq	NP_043143.1. NP_043261.1.
3D structure databases
SMR	Q36863. Positions 21-191.
ModBase	Search...
Genome annotation databases
GeneID	801572. 801684.
Enzyme and pathway databases
BRENDA	3.4.21.92. 11.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP1_CYAPA

Accession

Primary (citable) accession number: Q36863

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)