Q36450 (NDUS2_NICSY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
NADH dehydrogenase subunit 7

Gene names

Name:

NAD7

Encoded on

Mitochondrion

Organism

Nicotiana sylvestris (Wood tobacco) (South American tobacco)

Taxonomic identifier

4096 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. Component of the iron-sulfur (IP) fragment of the enzyme.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Subunit structure	Complex I is composed of about 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	NADH dehydrogenase [ubiquinone] iron-sulfur protein 2		PRO_0000118585

Sequences

	Sequence		Length	Mass (Da)
	Q36450 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4DCA5173BAC46258	FASTA	394	44,522
	10 20 30 40 50 60 MTTKNRQIKN FTSNFGPQHP AAHGVSRSVL EMNGEVVERA EPHIGLLQRG TEKLIEYKTY 70 80 90 100 110 120 LQALPYSDRS EYVSMMAQEH AHSSAVERLL NCEVPLRAQY IRVLFREITR ISNHSLALTT 130 140 150 160 170 180 HAMDVGASTP FLWAFEEREK LLEFYERVSG ARMHASFIRP GGVAQDLPLG LCIDIDSFTQ 190 200 210 220 230 240 QFASRIDELE EMSTGNRIWK QRLVDIGTVT AQQAKDWGFS GVMLRGSGVC WDLRKAAPYD 250 260 270 280 290 300 VHDQLDPDIP VGTRGDRYDR YCIRIEEMRQ SVRIIVQCLN QMPSGMIKAD DRKLCPPSRS 310 320 330 340 350 360 RMKLSMESSI HHFEPYTEGF SVPAPSTYTA VEAPKGEFGV FLVSNGSNRP YRRKIRAPCF 370 380 390 AHSQGLDSMS KHHMPADVVT IIGTQDIVSG EVDR

References

[1]

"Deletion of the last two exons of the mitochondrial nad7 gene results in lack of the NAD7 polypeptide in a Nicotiana sylvestris CMS mutant." Pla M., Mathieu C., De Paepe R., Chetrit P., Vedel F. Mol. Gen. Genet. 248:79-88(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X86706 mRNA. Translation: CAA60392.1.
3D structure databases
ProteinModelPortal	Q36450.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001135. NADH_Q_OxRdtase_suD. IPR022885. NDH1_su_D/H. [Graphical view]
Pfam	PF00346. Complex1_49kDa. 1 hit. [Graphical view]
TIGRFAMs	TIGR01962. NuoD. 1 hit.
PROSITE	PS00535. COMPLEX1_49K. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS2_NICSY

Accession

Primary (citable) accession number: Q36450

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program