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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2

Gene

NAD7

Organism
Nicotiana sylvestris (Wood tobacco) (South American tobacco)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). Component of the iron-sulfur (IP) fragment of the enzyme.By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  3. quinone binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
NADH dehydrogenase subunit 7
Gene namesi
Name:NAD7
Encoded oniMitochondrion
OrganismiNicotiana sylvestris (Wood tobacco) (South American tobacco)
Taxonomic identifieri4096 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394NADH dehydrogenase [ubiquinone] iron-sulfur protein 2PRO_0000118585Add
BLAST

Interactioni

Subunit structurei

Complex I is composed of about 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ36450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Curated

Family and domain databases

Gene3Di1.10.645.10. 2 hits.
HAMAPiMF_01358. NDH1_NuoD.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01962. NuoD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q36450-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTKNRQIKN FTSNFGPQHP AAHGVSRSVL EMNGEVVERA EPHIGLLQRG
60 70 80 90 100
TEKLIEYKTY LQALPYSDRS EYVSMMAQEH AHSSAVERLL NCEVPLRAQY
110 120 130 140 150
IRVLFREITR ISNHSLALTT HAMDVGASTP FLWAFEEREK LLEFYERVSG
160 170 180 190 200
ARMHASFIRP GGVAQDLPLG LCIDIDSFTQ QFASRIDELE EMSTGNRIWK
210 220 230 240 250
QRLVDIGTVT AQQAKDWGFS GVMLRGSGVC WDLRKAAPYD VHDQLDPDIP
260 270 280 290 300
VGTRGDRYDR YCIRIEEMRQ SVRIIVQCLN QMPSGMIKAD DRKLCPPSRS
310 320 330 340 350
RMKLSMESSI HHFEPYTEGF SVPAPSTYTA VEAPKGEFGV FLVSNGSNRP
360 370 380 390
YRRKIRAPCF AHSQGLDSMS KHHMPADVVT IIGTQDIVSG EVDR
Length:394
Mass (Da):44,522
Last modified:November 1, 1996 - v1
Checksum:i4DCA5173BAC46258
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86706 mRNA. Translation: CAA60392.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86706 mRNA. Translation: CAA60392.1.

3D structure databases

ProteinModelPortaliQ36450.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.645.10. 2 hits.
HAMAPiMF_01358. NDH1_NuoD.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01962. NuoD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Deletion of the last two exons of the mitochondrial nad7 gene results in lack of the NAD7 polypeptide in a Nicotiana sylvestris CMS mutant."
    Pla M., Mathieu C., De Paepe R., Chetrit P., Vedel F.
    Mol. Gen. Genet. 248:79-88(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Leaf.

Entry informationi

Entry nameiNDUS2_NICSY
AccessioniPrimary (citable) accession number: Q36450
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.