Q35101 (COX1_METSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase subunit 1
EC=1.9.3.1

Alternative name(s):
Cytochrome c oxidase polypeptide I

Gene names

Name:

COI

Encoded on

Mitochondrion

Organism

Metridium senile (Brown sea anemone) (Frilled sea anemone)

Taxonomic identifier

6116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Cnidaria › Anthozoa › Hexacorallia › Actiniaria › Nynantheae › Metridiidae › Metridium

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.
Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Pathway	Energy metabolism; oxidative phosphorylation.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the heme-copper respiratory oxidase family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
Ligand	Copper Heme Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	aerobic respiration Inferred from electronic annotation. Source: InterPro electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW oxidative phosphorylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: InterPro respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	copper ion binding Inferred from electronic annotation. Source: InterPro cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Cytochrome c oxidase subunit 1		PRO_0000183362
Regions
	Transmembrane	20 – 40	21	Helical; Potential
	Transmembrane	59 – 79	21	Helical; Potential
	Transmembrane	105 – 125	21	Helical; Potential
	Transmembrane	148 – 168	21	Helical; Potential
	Transmembrane	186 – 206	21	Helical; Potential
	Transmembrane	237 – 257	21	Helical; Potential
	Transmembrane	270 – 290	21	Helical; Potential
	Transmembrane	313 – 333	21	Helical; Potential
	Transmembrane	341 – 361	21	Helical; Potential
	Transmembrane	380 – 400	21	Helical; Potential
	Transmembrane	417 – 437	21	Helical; Potential
	Transmembrane	455 – 475	21	Helical; Potential
Sites
	Metal binding	64	1	Iron (heme A axial ligand) Probable
	Metal binding	243	1	Copper B Probable
	Metal binding	247	1	Copper B Probable
	Metal binding	293	1	Copper B Probable
	Metal binding	294	1	Copper B Probable
	Metal binding	379	1	Iron (heme A3 axial ligand) Probable
	Metal binding	381	1	Iron (heme A axial ligand) Probable
Amino acid modifications
	Cross-link	243 ↔ 247		1'-histidyl-3'-tyrosine (His-Tyr) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q35101 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CBD28A5AF94113A5	FASTA	530	58,770
	10 20 30 40 50 60 MDNKFLTRWV FSTNHKDIGT LYLVFGIGSG MIGTALSMLI RLELSAPGTM LGDDHLYNVI 70 80 90 100 110 120 VTAHAFIMIF FLVMPVMIGG FGNWLVPLYI GAPDMAFPRL NNISFWLLPP ALILLLGSAF 130 140 150 160 170 180 VEQGVGTGWT VYPPLSAIQA HSGGAVDMAI FSLHLAGASS ILGAMNFITT IFNMRAPGMT 190 200 210 220 230 240 MDRLPLFVWS ILITAFLLLL SLPVLAGAIT MLLTDRNFNT TFFDPAGGGD PILFQHLFWF 250 260 270 280 290 300 FGHPEVYILI LPGFGMVSQI IPTFSAKNQI FGYLGMVYAM LSIGILGFIV WAHHMFTVGM 310 320 330 340 350 360 DVDTRAYFTA ATMIIAVPTG IKVFSWLATI YGGAVRLDTP MLWAIGFVFL FTIGGLTGVI 370 380 390 400 410 420 LANSSLDIVL HDTYYVVAHF HYVLSMGAIF AIFAGFYFWF GKITGYCYNE LYGKIHFWIM 430 440 450 460 470 480 FIGVNLTFFP QHFLGLAGFP RRYSDFVDGF AGWNLVSSLG STISIVGVIW FVFIVYDAYV 490 500 510 520 530 REIKFIGWVE NTGASWSSLE WVQPSPPMSH TYNELPFVYG SYSALRPRNS

References

[1]	"Two mitochondrial group I introns in a metazoan, the sea anemone Metridium senile: one intron contains genes for subunits 1 and 3 of NADH dehydrogenase." Beagley C.T., Okada N.A., Wolstenholme D.R. Proc. Natl. Acad. Sci. U.S.A. 93:5619-5623(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: White morph.
[2]	Beagley C.T., Okimoto R., Wolstenholme D.R. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: White morph.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U36783 Genomic DNA. Translation: AAC47099.1. AF000023 Genomic DNA. Translation: AAC04630.1.
PIR	T11884.
RefSeq	NP_009253.1. NC_000933.1.
3D structure databases
ProteinModelPortal	Q35101.
SMR	Q35101. Positions 5-518.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	808778.
Organism-specific databases
CTD	4512.
Phylogenomic databases
ProtClustDB	MTH00182.
Enzyme and pathway databases
UniPathway	UPA00705.
Family and domain databases
Gene3D	1.20.210.10. 1 hit.
InterPro	IPR000883. Cyt_c_Oxase_su1. IPR023615. Cyt_c_Oxase_su1_BS. IPR023616. Cyt_c_Oxase_su1_dom. IPR014241. Cyt_c_oxidase_su1_bac. [Graphical view]
PANTHER	PTHR10422. PTHR10422. 1 hit.
Pfam	PF00115. COX1. 1 hit. [Graphical view]
PRINTS	PR01165. CYCOXIDASEI.
SUPFAM	SSF81442. COX1. 1 hit.
TIGRFAMs	TIGR02891. CtaD_CoxA. 1 hit.
PROSITE	PS50855. COX1. 1 hit. PS00077. COX1_CUB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COX1_METSE

Accession

Primary (citable) accession number: Q35101

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)