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Q34312

- NDUS1_DICDI

UniProt

Q34312 - NDUS1_DICDI

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Protein
NADH-ubiquinone oxidoreductase 75 kDa subunit
Gene
nad11, ndufs1, DDB_G0294052
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Binds 1 2Fe-2S cluster per subunit By similarity.
Binds 2 4Fe-4S clusters per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi38 – 381Iron-sulfur 1 (2Fe-2S) By similarity
Metal bindingi49 – 491Iron-sulfur 1 (2Fe-2S) By similarity
Metal bindingi52 – 521Iron-sulfur 1 (2Fe-2S) By similarity
Metal bindingi66 – 661Iron-sulfur 1 (2Fe-2S) By similarity
Metal bindingi101 – 1011Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
Metal bindingi105 – 1051Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi108 – 1081Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi114 – 1141Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi153 – 1531Iron-sulfur 3 (4Fe-4S) By similarity
Metal bindingi156 – 1561Iron-sulfur 3 (4Fe-4S) By similarity
Metal bindingi159 – 1591Iron-sulfur 3 (4Fe-4S) By similarity
Metal bindingi204 – 2041Iron-sulfur 3 (4Fe-4S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. NADH dehydrogenase (ubiquinone) activity Source: RefGenome
  4. electron carrier activity Source: InterPro
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. ATP synthesis coupled electron transport Source: InterPro
  2. cellular respiration Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-75kD
Short name:
CI-75kD
Gene namesi
Name:nad11
Synonyms:ndufs1
ORF Names:DDB_G0294052
Encoded oniMitochondrion
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Mitochondrion

Organism-specific databases

dictyBaseiDDB_G0294052. nad11.

Subcellular locationi

Mitochondrion inner membrane By similarity
Note: Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity.

GO - Cellular componenti

  1. mitochondrial respiratory chain complex I Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 688688NADH-ubiquinone oxidoreductase 75 kDa subunit
PRO_0000327377Add
BLAST

Interactioni

Subunit structurei

Complex I is composed of about 45 different subunits By similarity.

Protein-protein interaction databases

STRINGi44689.DidioMp37.

Structurei

3D structure databases

ProteinModelPortaliQ34312.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 85852Fe-2S ferredoxin-type
Add
BLAST
Domaini223 – 279574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1034.
KOiK03934.
OMAiINEIECE.
PhylomeDBiQ34312.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
[Graphical view]
PfamiPF00111. Fer2. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMiSSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR01973. NuoG. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q34312-1 [UniParc]FASTAAdd to Basket

« Hide

MIIRFKINEI ECEVNEEKED ITILQACTAN GIEIPRFCYH EKLTIAGNCR    50
MCLVYVTNEE KLLAACGIPL DENFDDESIE TEIDEILKAR EGVMEFLLIN 100
HPLDCPICDQ GGECDLQEQT LAYGLDTGRF YIKKRAVEIK TFGRLIKGIM 150
TRCIHCTRCV RFLTEIAGVN ELGVLGRGYN MEIGTYKKNV MIESELSGNI 200
IDLCPVGALT SAVYAYKGRP WELKNIKGID IFDTLLTPIN YQVKGGEIFR 250
ILPRINDRIN EEWITDKVRF HYESYKIIEK IRKETPSYKI QANKFIELTW 300
KTALKMVFKV LLNKKNKVDL IIGSKINSTN LRIYKELMNR LGSKNYITEN 350
GLMFKKFNYD LRENYINSND LYNVDKNDLV LLCGINLRVE SPLLNIKLRN 400
VNFGDDEIES VKKIGIIGNK FDWKHESEYI GATLNSMLKL FEGRLPYCQQ 450
IKKSKAPLII VGPSLLTRIS LTLQEMRAIF MKACNLKPEN ILIITQGANF 500
GMALEEGLFK EKFSIGGNVL YSIDSNEVQV TNKINYVIYQ GIINDKFENK 550
IDLYLPSKHY FEDFEGDREV YMNTFGQRSE IEKLSISKGN KIKENSMIGY 600
IQLMYLNNKE MTRKEKEQKD IKLSYREMKK EEKRKIKVNK YLTINNIIEN 650
YYMTDINIRL SKNLMITGQL RKEKKIMEAG IWKNRKCI 688
Length:688
Mass (Da):79,838
Last modified:November 1, 1996 - v1
Checksum:i7091BF41C8A055F6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U02291 Genomic DNA. Translation: AAA77667.1.
AB000109 Genomic DNA. Translation: BAA78086.1.
PIRiT43783.
RefSeqiNP_050104.1. NC_000895.1.

Genome annotation databases

GeneIDi2193950.
KEGGiddi:DidioMp37.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U02291 Genomic DNA. Translation: AAA77667.1 .
AB000109 Genomic DNA. Translation: BAA78086.1 .
PIRi T43783.
RefSeqi NP_050104.1. NC_000895.1.

3D structure databases

ProteinModelPortali Q34312.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DidioMp37.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2193950.
KEGGi ddi:DidioMp37.

Organism-specific databases

dictyBasei DDB_G0294052. nad11.

Phylogenomic databases

eggNOGi COG1034.
KOi K03934.
OMAi INEIECE.
PhylomeDBi Q34312.

Miscellaneous databases

PROi Q34312.

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
[Graphical view ]
Pfami PF00111. Fer2. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SMARTi SM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SUPFAMi SSF54292. SSF54292. 1 hit.
TIGRFAMsi TIGR01973. NuoG. 1 hit.
PROSITEi PS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Dictyostelium discoideum mitochondrial genome: a primordial system using the universal code and encoding hydrophilic proteins atypical of metazoan mitochondrial DNA."
    Cole R.A., Williams K.L.
    J. Mol. Evol. 39:579-588(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: X22.
  2. "The mitochondrial DNA of Dictyostelium discoideum: complete sequence, gene content and genome organization."
    Ogawa S., Yoshino R., Angata K., Iwamoto M., Pi M., Kuroe K., Matsuo K., Morio T., Urushihara H., Yanagisawa K., Tanaka Y.
    Mol. Gen. Genet. 263:514-519(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX3.

Entry informationi

Entry nameiNDUS1_DICDI
AccessioniPrimary (citable) accession number: Q34312
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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