NADH-ubiquinone oxidoreductase 75 kDa subunit - Dictyostelium discoideum (Slime mold)

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Q34312 (NDUS1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 84. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
Complex I-75kD
Short name=CI-75kD

Gene names

Name:	nad11
Synonyms:	ndufs1
ORF Names:	DDB_G0294052

Encoded on

Mitochondrion

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	688 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.
Catalytic activity	NADH + ubiquinone = NAD⁺ + ubiquinol. NADH + acceptor = NAD⁺ + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	Complex I is composed of about 45 different subunits By similarity.
Subcellular location	Mitochondrion inner membrane By similarity. Note: Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity.
Sequence similarities	Belongs to the complex I 75 kDa subunit family. Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro cellular respiration Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	mitochondrial respiratory chain complex I Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from Biological aspect of Ancestor. Source: RefGenome electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 688

688

NADH-ubiquinone oxidoreductase 75 kDa subunit

PRO_0000327377

Regions

Domain

1 – 85

2Fe-2S ferredoxin-type

Sites

Metal binding

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

101

Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity

Metal binding

105

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

108

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

114

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

153

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

156

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

159

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

204

Iron-sulfur 3 (4Fe-4S) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q34312 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7091BF41C8A055F6
Show »

FASTA

688

79,838

        10         20         30         40         50         60 
MIIRFKINEI ECEVNEEKED ITILQACTAN GIEIPRFCYH EKLTIAGNCR MCLVYVTNEE 

        70         80         90        100        110        120 
KLLAACGIPL DENFDDESIE TEIDEILKAR EGVMEFLLIN HPLDCPICDQ GGECDLQEQT 

       130        140        150        160        170        180 
LAYGLDTGRF YIKKRAVEIK TFGRLIKGIM TRCIHCTRCV RFLTEIAGVN ELGVLGRGYN 

       190        200        210        220        230        240 
MEIGTYKKNV MIESELSGNI IDLCPVGALT SAVYAYKGRP WELKNIKGID IFDTLLTPIN 

       250        260        270        280        290        300 
YQVKGGEIFR ILPRINDRIN EEWITDKVRF HYESYKIIEK IRKETPSYKI QANKFIELTW 

       310        320        330        340        350        360 
KTALKMVFKV LLNKKNKVDL IIGSKINSTN LRIYKELMNR LGSKNYITEN GLMFKKFNYD 

       370        380        390        400        410        420 
LRENYINSND LYNVDKNDLV LLCGINLRVE SPLLNIKLRN VNFGDDEIES VKKIGIIGNK 

       430        440        450        460        470        480 
FDWKHESEYI GATLNSMLKL FEGRLPYCQQ IKKSKAPLII VGPSLLTRIS LTLQEMRAIF 

       490        500        510        520        530        540 
MKACNLKPEN ILIITQGANF GMALEEGLFK EKFSIGGNVL YSIDSNEVQV TNKINYVIYQ 

       550        560        570        580        590        600 
GIINDKFENK IDLYLPSKHY FEDFEGDREV YMNTFGQRSE IEKLSISKGN KIKENSMIGY 

       610        620        630        640        650        660 
IQLMYLNNKE MTRKEKEQKD IKLSYREMKK EEKRKIKVNK YLTINNIIEN YYMTDINIRL 

       670        680 
SKNLMITGQL RKEKKIMEAG IWKNRKCI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Dictyostelium discoideum mitochondrial genome: a primordial system using the universal code and encoding hydrophilic proteins atypical of metazoan mitochondrial DNA." Cole R.A., Williams K.L. J. Mol. Evol. 39:579-588(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: X22.
[2]	"The mitochondrial DNA of Dictyostelium discoideum: complete sequence, gene content and genome organization." Ogawa S., Yoshino R., Angata K., Iwamoto M., Pi M., Kuroe K., Matsuo K., Morio T., Urushihara H., Yanagisawa K., Tanaka Y. Mol. Gen. Genet. 263:514-519(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U02291 Genomic DNA. Translation: AAA77667.1. AB000109 Genomic DNA. Translation: BAA78086.1.
PIR	T43783.
RefSeq	NP_050104.1. NC_000895.1.
3D structure databases
HSSP	HSSP built from PDB template 2FUG based on UniProtKB Q56223.
ProteinModelPortal	Q34312.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DidioMp37.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2193950.
KEGG	ddi:DidioMp37.
Organism-specific databases
dictyBase	DDB_G0294052. nad11.
Phylogenomic databases
eggNOG	COG1034.
KO	K03934.
OMA	INEIECE.
Family and domain databases
Gene3D	3.10.20.30. 1 hit.
InterPro	IPR001041. 2Fe-2S_ferredoxin-type. IPR012675. Beta-grasp_dom. IPR006656. Mopterin_OxRdtase. IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS. IPR010228. NADH_UbQ_OxRdtase_Gsu. IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd. [Graphical view]
Pfam	PF00111. Fer2. 1 hit. PF00384. Molybdopterin. 1 hit. PF10588. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
SMART	SM00929. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
SUPFAM	SSF54292. Ferredoxin. 1 hit.
TIGRFAMs	TIGR01973. NuoG. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00641. COMPLEX1_75K_1. 1 hit. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS1_DICDI

Accession

Primary (citable) accession number: Q34312

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents