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Q34312 (NDUS1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit

EC=1.6.5.3
EC=1.6.99.3
Alternative name(s):
Complex I-75kD
Short name=CI-75kD
Gene names
Name:nad11
Synonyms:ndufs1
ORF Names:DDB_G0294052
Encoded onMitochondrion
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length688 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.

Catalytic activity

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).

NADH + acceptor = NAD+ + reduced acceptor.

Cofactor

Binds 1 2Fe-2S cluster per subunit By similarity.

Binds 2 4Fe-4S clusters per subunit By similarity.

Subunit structure

Complex I is composed of about 45 different subunits By similarity.

Subcellular location

Mitochondrion inner membrane By similarity. Note: Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity.

Sequence similarities

Belongs to the complex I 75 kDa subunit family.

Contains 1 2Fe-2S ferredoxin-type domain.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 688688NADH-ubiquinone oxidoreductase 75 kDa subunit
PRO_0000327377

Regions

Domain1 – 85852Fe-2S ferredoxin-type
Domain223 – 279574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding381Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding491Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding521Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding661Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding1011Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
Metal binding1051Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1081Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1141Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1531Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1561Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1591Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding2041Iron-sulfur 3 (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q34312 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7091BF41C8A055F6

FASTA68879,838
        10         20         30         40         50         60 
MIIRFKINEI ECEVNEEKED ITILQACTAN GIEIPRFCYH EKLTIAGNCR MCLVYVTNEE 

        70         80         90        100        110        120 
KLLAACGIPL DENFDDESIE TEIDEILKAR EGVMEFLLIN HPLDCPICDQ GGECDLQEQT 

       130        140        150        160        170        180 
LAYGLDTGRF YIKKRAVEIK TFGRLIKGIM TRCIHCTRCV RFLTEIAGVN ELGVLGRGYN 

       190        200        210        220        230        240 
MEIGTYKKNV MIESELSGNI IDLCPVGALT SAVYAYKGRP WELKNIKGID IFDTLLTPIN 

       250        260        270        280        290        300 
YQVKGGEIFR ILPRINDRIN EEWITDKVRF HYESYKIIEK IRKETPSYKI QANKFIELTW 

       310        320        330        340        350        360 
KTALKMVFKV LLNKKNKVDL IIGSKINSTN LRIYKELMNR LGSKNYITEN GLMFKKFNYD 

       370        380        390        400        410        420 
LRENYINSND LYNVDKNDLV LLCGINLRVE SPLLNIKLRN VNFGDDEIES VKKIGIIGNK 

       430        440        450        460        470        480 
FDWKHESEYI GATLNSMLKL FEGRLPYCQQ IKKSKAPLII VGPSLLTRIS LTLQEMRAIF 

       490        500        510        520        530        540 
MKACNLKPEN ILIITQGANF GMALEEGLFK EKFSIGGNVL YSIDSNEVQV TNKINYVIYQ 

       550        560        570        580        590        600 
GIINDKFENK IDLYLPSKHY FEDFEGDREV YMNTFGQRSE IEKLSISKGN KIKENSMIGY 

       610        620        630        640        650        660 
IQLMYLNNKE MTRKEKEQKD IKLSYREMKK EEKRKIKVNK YLTINNIIEN YYMTDINIRL 

       670        680 
SKNLMITGQL RKEKKIMEAG IWKNRKCI 

« Hide

References

« Hide 'large scale' references
[1]"The Dictyostelium discoideum mitochondrial genome: a primordial system using the universal code and encoding hydrophilic proteins atypical of metazoan mitochondrial DNA."
Cole R.A., Williams K.L.
J. Mol. Evol. 39:579-588(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: X22.
[2]"The mitochondrial DNA of Dictyostelium discoideum: complete sequence, gene content and genome organization."
Ogawa S., Yoshino R., Angata K., Iwamoto M., Pi M., Kuroe K., Matsuo K., Morio T., Urushihara H., Yanagisawa K., Tanaka Y.
Mol. Gen. Genet. 263:514-519(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U02291 Genomic DNA. Translation: AAA77667.1.
AB000109 Genomic DNA. Translation: BAA78086.1.
PIRT43783.
RefSeqNP_050104.1. NC_000895.1.

3D structure databases

ProteinModelPortalQ34312.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DidioMp37.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2193950.
KEGGddi:DidioMp37.

Organism-specific databases

dictyBaseDDB_G0294052. nad11.

Phylogenomic databases

eggNOGCOG1034.
KOK03934.
OMAINEIECE.
PhylomeDBQ34312.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
[Graphical view]
PfamPF00111. Fer2. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SMARTSM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMSSF54292. SSF54292. 1 hit.
TIGRFAMsTIGR01973. NuoG. 1 hit.
PROSITEPS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ34312.

Entry information

Entry nameNDUS1_DICDI
AccessionPrimary (citable) accession number: Q34312
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase