Reviewed, UniProtKB/Swiss-Prot Q33BW7 (NU1C_NICTO)

Names and origin

Protein names

Recommended name:
    NAD(P)H-quinone oxidoreductase subunit 1, chloroplastic
    EC=1.6.5.-
Alternative name(s):
    NAD(P)H dehydrogenase subunit 1
      Short name=NDH subunit 1
    NADH-plastoquinone oxidoreductase subunit 1

Gene names

Name:

ndhA

Encoded on

Plastid; Chloroplast

Organism

Nicotiana tomentosiformis (Tobacco)

Taxonomic identifier

4098 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NAD(P)H + plastoquinone = NAD(P)+ + plastoquinol. HAMAP MF_01350
Subunit structure	NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus By similarity.
Subcellular location	Plastid › chloroplast thylakoid membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transmembrane
Ligand	NAD NADP Plastoquinone
Molecular function	Oxidoreductase
PTM	Quinone
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP
Cellular component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	NAD(P)H-quinone oxidoreductase subunit 1, chloroplastic HAMAP MF_01350		PRO_0000240027
Regions
	Transmembrane	30 – 50	21	Potential
	Transmembrane	98 – 118	21	Potential
	Transmembrane	127 – 147	21	Potential
	Transmembrane	165 – 185	21	Potential
	Transmembrane	203 – 223	21	Potential
	Transmembrane	248 – 268	21	Potential
	Transmembrane	300 – 320	21	Potential
	Transmembrane	336 – 356	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q33BW7-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 55B9D8D45C9D3A67	FASTA	363	40,094
	10 20 30 40 50 60 MIIDTTEIET INSFSKLESL KEVYGIIWML FPIFTLVLGI TIGVLVIVWL EREISAGIQQ 70 80 90 100 110 120 RIGPEYAGPL GILQALADGT KLLLKENLIP STGDTRLFSI GPSIAVISIF LSYSVIPFGD 130 140 150 160 170 180 HLVLADLSIG VFFWIAISSI APVGLLMSGY GSNNKYSFLG GLRAAAQSIS YEIPLALCVL 190 200 210 220 230 240 SISLLSNSSS TVDIVEAQSK YGFWGWNLWR QPIGFIVFLI SSLAECERLP FDLPEAEEEL 250 260 270 280 290 300 VAGYQTEYSG IKFGLFYIAS YLNLLVSSLF VTVLYLGGWN LSIPYIFVPE LFGINKKGKV 310 320 330 340 350 360 FGTLIGIFIT LAKTYLFLFI PIATRWTLPR LRMDQLLNLG WKFLLPISLG NLLLTTSSQL LSL

References

[1]

"The chloroplast genome of Nicotiana sylvestris and Nicotiana tomentosiformis: complete sequencing confirms that the Nicotiana sylvestris progenitor is the maternal genome donor of Nicotiana tabacum." Yukawa M., Tsudzuki T., Sugiura M. Mol. Genet. Genomics 275:367-373(2006) [PubMed: 16435119] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB240139 Genomic DNA. Translation: BAE48068.1.
RefSeq	YP_398928.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3776346.
Family and domain databases
HAMAP	MF_01350. [Tree]
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NU1C_NICTO

Accession

Primary (citable) accession number: Q33BW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	December 6, 2005
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)