Reviewed, UniProtKB/Swiss-Prot Q339K6 (LAC15_ORYSJ)

Names and origin

Protein names

Recommended name:
    Laccase-15
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 15
    Urishiol oxidase 15
    Diphenol oxidase 15

Gene names

Name:	LAC15
Ordered Locus Names:	Os10g0346300, LOC_Os10g20610
ORF Names:	OSJNBa0045C13.15

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted › extracellular space › apoplastPotential.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.
Sequence caution	The sequence AAK92654.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Apoplast Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: InterPro laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 599	570	Laccase-15		PRO_0000291899
Regions
	Domain	46 – 162	117	Plastocyanin-like 1
	Domain	173 – 328	156	Plastocyanin-like 2
	Domain	444 – 586	143	Plastocyanin-like 3
Sites
	Metal binding	96	1	Copper 1 By similarity
	Metal binding	98	1	Copper 2 By similarity
	Metal binding	141	1	Copper 2 By similarity
	Metal binding	143	1	Copper 3 By similarity
	Metal binding	503	1	Copper 4 Potential
	Metal binding	506	1	Copper 1 By similarity
	Metal binding	508	1	Copper 3 By similarity
	Metal binding	565	1	Copper 3 By similarity
	Metal binding	566	1	Copper 4 Potential
	Metal binding	567	1	Copper 2 By similarity
	Metal binding	571	1	Copper 4 Potential
	Metal binding	576	1	Copper 4 Potential
Amino acid modifications
	Glycosylation	51	1	N-linked (GlcNAc...) Potential
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	124	1	N-linked (GlcNAc...) Potential
	Glycosylation	193	1	N-linked (GlcNAc...) Potential
	Glycosylation	217	1	N-linked (GlcNAc...) Potential
	Glycosylation	331	1	N-linked (GlcNAc...) Potential
	Glycosylation	355	1	N-linked (GlcNAc...) Potential
	Glycosylation	412	1	N-linked (GlcNAc...) Potential
	Glycosylation	454	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	273	1	T → A in AK110474. Ref.4
	Sequence conflict	311	1	D → N in AK110474. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q339K6-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 7E10A0E08196CFDF	FASTA	599	65,670
	10 20 30 40 50 60 MKRCQSSRPT AAVAAVVAAV SMIIVLVSGT AIPSAAAAAA VEHTFVVSQV NMTHLCKEMA 70 80 90 100 110 120 FTVVNGQLPG PTIEVTEGDS VTVHVVNKSP YNLTIHWHGV YQLLNCWNDG VPMITQRPIQ 130 140 150 160 170 180 PNHNFTYRFN VAGQEGTLWW HAHDAFLRGT VHGALIIRPR HGAASYPFPR PHREVPIIIG 190 200 210 220 230 240 EWWEKDLPQV DRNMTNGYFD DYSSGSTING KLGDLFNCSG VLEDGYVLDV EPGKTYLLRI 250 260 270 280 290 300 INAALFSEYF LKIAGHRFTV VASDANYLTP YSTDVVVIAP GETLDAIVVA DAPPSGRYYI 310 320 330 340 350 360 AAQPIQAPPP DTQTPEYATR GTLQYSSNSR NSSAAAMPEM PHQHDTMRSF YFRGNLTAGA 370 380 390 400 410 420 RLHRHGRRRV PARADESLFV TLGLGSVCRH GGASCKRGGN LKESIVVANV NNVSFHIPAA 430 440 450 460 470 480 AATPILEAHY YHRLHAGAGE EEEELAERPP RAYNYTDQAL TPFGPEEMRL EATSRAVVTR 490 500 510 520 530 540 RFRHGATVDV VFQSTAMLQG DSNPMHLHGH DVFLLAQGIG IYDAARDEGK FNLVNPPRKN 550 560 570 580 590 TVLVPNLGWA AVRFVADNPG AWLMHCHFEF HLSMGMAAVF IVEDGPTVDT SLPPPPEDF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"In-depth view of structure, activity, and evolution of rice chromosome 10." Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H. , Paradkar M., Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F., Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M., Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R., Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F., Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D., Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R., Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K., Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S., Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S., Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R., Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M., Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R., Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E., Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A. Science 300:1566-1569(2003) [PubMed: 12791992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC079634 Genomic DNA. Translation: AAK92654.1. Sequence problems. DP000086 Genomic DNA. Translation: ABB47271.1. AP008216 Genomic DNA. Translation: BAF26313.1. AK110474 mRNA. No translation available.
RefSeq	NP_001064399.1.
UniGene	Os.46813
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4348374.
KEGG	osa:4348374.
Organism-specific databases
Gramene	Q339K6.
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
PROSITE	PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC15_ORYSJ

Accession

Primary (citable) accession number: Q339K6
Secondary accession number(s): Q94HD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	December 6, 2005
Last modified:	November 25, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)