Reviewed, UniProtKB/Swiss-Prot Q33994 (NDUS3_BETTR)

Names and origin

Protein names

Recommended name:
    NADH-ubiquinone oxidoreductase 27 kDa subunit
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    Complex I-27kD
      Short name=CI-27kD
    NADH dehydrogenase subunit 9

Gene names

Name:

NAD9

Encoded on

Mitochondrion

Organism

Beta trigyna (Beet)

Taxonomic identifier

19769 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Beta

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Subunit structure	Complex I is composed of about 30 different subunits.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Sequence similarities	Belongs to the complex I 30 kDa subunit family.
RNA editing	Edited at positions 31, 38, 100, 110 and 123. By similarity.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	RNA editing
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 192	192	NADH-ubiquinone oxidoreductase 27 kDa subunit		PRO_0000118638

Sequences

	Sequence		Length	Mass (Da)
	Q33994-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 12C3F3F5AA32A925	FASTA	192	23,109
	10 20 30 40 50 60 MDNQFIFKYS WETLPKKWVK KIEKSEHGNR FDTNTDYLFQ LLCFLKLHTY TRFQVLIDIC 70 80 90 100 110 120 GVDYPSRKRR FEVVYNLLST RYNSRIRLQT SADEVTRISS VVSLFPSAGW WEREVWDMFG 130 140 150 160 170 180 VSFINHPDLR RILTDYGFEG HPLRKDFPLS GYVEVRYDDP EKRVVSEPIE MTQEFRYFDF 190 ASPWEQRNGN EG

References

[1]

"The chloroplast trnP-trnW-petG gene cluster in the mitochondrial genomes of Beta vulgaris, B. trigyna and B. webbiana: evolutionary aspects." Kubo T., Yanai Y., Kinoshita T., Mikami T. Curr. Genet. 27:285-289(1995) [PubMed: 7736615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. SP753012-O. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D38017 Genomic DNA. Translation: BAA07214.1. Sequence problems.
3D structure databases
SMR	Q33994. Positions 22-181.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.6.5.3. 312056. 1.6.99.3. 312056.
Family and domain databases
InterPro	IPR010218. NADH_DH_csu. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR020396. NADH_UbQ_OxRdtase_CS. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. [Graphical view]
ProDom	PD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01961. NuoC_fam. 1 hit.
PROSITE	PS00542. COMPLEX1_30K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS3_BETTR

Accession

Primary (citable) accession number: Q33994

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	February 9, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)