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Protein

Mitogen-activated protein kinase 6

Gene

MPK6

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72ATPPROSITE-ProRule annotation1
Active sitei169Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi49 – 57ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-OSA-110056. MAPK3 (ERK1) activation.
R-OSA-112409. RAF-independent MAPK1/3 activation.
R-OSA-112411. MAPK1 (ERK2) activation.
R-OSA-198753. ERK/MAPK targets.
R-OSA-198765. Signalling to ERK5.
R-OSA-202670. ERKs are inactivated.
R-OSA-3371453. Regulation of HSF1-mediated heat shock response.
R-OSA-442742. CREB phosphorylation through the activation of Ras.
R-OSA-445144. Signal transduction by L1.
R-OSA-5673001. RAF/MAP kinase cascade.
R-OSA-5674135. MAP2K and MAPK activation.
R-OSA-5674499. Negative feedback regulation of MAPK pathway.
R-OSA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 6 (EC:2.7.11.24)
Short name:
MAP kinase 6
Gene namesi
Name:MPK6
Ordered Locus Names:Os10g0533600, LOC_Os10g38950
ORF Names:OSJNBa0053C23.4
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 10

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002397491 – 376Mitogen-activated protein kinase 6Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei201PhosphothreonineBy similarity1
Modified residuei203PhosphotyrosineCurated1

Post-translational modificationi

Dually phosphorylated on Thr-201 and Tyr-203, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ336X9.
PRIDEiQ336X9.

Expressioni

Gene expression databases

GenevisibleiQ336X9. OS.

Interactioni

Protein-protein interaction databases

IntActiQ336X9. 1 interactor.
MINTiMINT-7234445.
STRINGi39947.LOC_Os10g38950.1.

Structurei

3D structure databases

ProteinModelPortaliQ336X9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 329Protein kinasePROSITE-ProRule annotationAdd BLAST287

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi201 – 203TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ336X9.
KOiK04464.
OMAiTEDNQGF.
OrthoDBiEOG09360BXQ.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q336X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSSSGGAGG GGGAQIKGMG THGGRYVLYN VYGNFFEVSS KYAPPIRPIG
60 70 80 90 100
RGAYGIVCAA VNSENGEEVA IKKIGNAFDN HIDAKRTLRE IKLLRHMDHE
110 120 130 140 150
NIIAIKDIIR PPRRDNFNDV YIVSELMDTD LHQIIRSNQP LTDDHCQYFL
160 170 180 190 200
YQLLRGLKYV HSANVLHRDL KPSNLFLNAN CDLKIADFGL ARTTTETDLM
210 220 230 240 250
TEYVVTRWYR APELLLNCSQ YTAAIDVWSV GCILGEIVTR QPLFPGRDYI
260 270 280 290 300
QQLKLITELI GSPDDSSLGF LRSDNARRYM KQLPQYPRQD FRLRFRNMSA
310 320 330 340 350
GAVDLLEKML VFDPSRRITV DEALHHPYLA SLHDINEEPT CPAPFSFDFE
360 370
QPSFTEEHIK ELIWRESLAF NPDPPY
Length:376
Mass (Da):42,773
Last modified:December 6, 2005 - v1
Checksum:i700EC7AAEE91AA45
GO

Sequence cautioni

The sequence AAM88622 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AK111579 differs from that shown. Reason: Frameshift at position 359.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC092389 Genomic DNA. Translation: AAM88622.1. Sequence problems.
DP000086 Genomic DNA. Translation: ABB47925.1.
AP008216 Genomic DNA. Translation: BAF27070.1.
AP014966 Genomic DNA. Translation: BAT11800.1.
AK111579 mRNA. No translation available.
RefSeqiXP_015615011.1. XM_015759525.1.
UniGeneiOs.15044.

Genome annotation databases

EnsemblPlantsiOS10T0533600-01; OS10T0533600-01; OS10G0533600.
GeneIDi4349225.
GrameneiOS10T0533600-01; OS10T0533600-01; OS10G0533600.
KEGGiosa:4349225.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC092389 Genomic DNA. Translation: AAM88622.1. Sequence problems.
DP000086 Genomic DNA. Translation: ABB47925.1.
AP008216 Genomic DNA. Translation: BAF27070.1.
AP014966 Genomic DNA. Translation: BAT11800.1.
AK111579 mRNA. No translation available.
RefSeqiXP_015615011.1. XM_015759525.1.
UniGeneiOs.15044.

3D structure databases

ProteinModelPortaliQ336X9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ336X9. 1 interactor.
MINTiMINT-7234445.
STRINGi39947.LOC_Os10g38950.1.

Proteomic databases

PaxDbiQ336X9.
PRIDEiQ336X9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS10T0533600-01; OS10T0533600-01; OS10G0533600.
GeneIDi4349225.
GrameneiOS10T0533600-01; OS10T0533600-01; OS10G0533600.
KEGGiosa:4349225.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ336X9.
KOiK04464.
OMAiTEDNQGF.
OrthoDBiEOG09360BXQ.

Enzyme and pathway databases

ReactomeiR-OSA-110056. MAPK3 (ERK1) activation.
R-OSA-112409. RAF-independent MAPK1/3 activation.
R-OSA-112411. MAPK1 (ERK2) activation.
R-OSA-198753. ERK/MAPK targets.
R-OSA-198765. Signalling to ERK5.
R-OSA-202670. ERKs are inactivated.
R-OSA-3371453. Regulation of HSF1-mediated heat shock response.
R-OSA-442742. CREB phosphorylation through the activation of Ras.
R-OSA-445144. Signal transduction by L1.
R-OSA-5673001. RAF/MAP kinase cascade.
R-OSA-5674135. MAP2K and MAPK activation.
R-OSA-5674499. Negative feedback regulation of MAPK pathway.
R-OSA-6798695. Neutrophil degranulation.

Gene expression databases

GenevisibleiQ336X9. OS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPK6_ORYSJ
AccessioniPrimary (citable) accession number: Q336X9
Secondary accession number(s): A0A0P0XX33, Q0IW45, Q8LN33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: December 6, 2005
Last modified: November 30, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.