Q336M7 (4CLL2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-coumarate--CoA ligase-like 2
EC=6.2.1.-

Gene names

Name:	4CLL2
Ordered Locus Names:	Os10g0578950, LOC_Os10g42800
ORF Names:	OSJNBa0056G17.30, OsJ_031306

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Domain	Both substrate-binding domains (SBD1 and SBD2) are involved in the substrate recognition, and are sufficient to confer the substrate specificity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.
Sequence caution	The sequence AAG46175.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence ABB48038.2 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAZ17097.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 583	583	4-coumarate--CoA ligase-like 2		PRO_0000351628
Regions
	Nucleotide binding	207 – 215	9	ATP By similarity
	Nucleotide binding	349 – 354	6	ATP By similarity
	Region	281 – 348	68	SBD1
	Region	349 – 419	71	SBD2
Sites
	Binding site	438	1	ATP By similarity
	Binding site	453	1	ATP By similarity
Experimental info
	Sequence conflict	343	1	H → N in EAZ17097. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q336M7 [UniParc]. Last modified October 14, 2008. Version 3. Checksum: F707063E656431BA	FASTA	583	63,329
	10 20 30 40 50 60 MQPDAAATAH PSLSFYSAAT GLYSSLHPPL PLPSDPSLSL VPHLFSHLPL HHHSLLVDAP 70 80 90 100 110 120 TAATLSCADF RRLVSSLAAG LRRRLHIARG SLVLLLLPNS LTFPVAFLAV LATGAVATTM 130 140 150 160 170 180 NPSSAPAEIA ARLRDTAPSL VLASTHNAAK LPPLAAPLVL VPDTFQQQHD DDQFDFFFHA 190 200 210 220 230 240 LLETDPETPV EMGVGVGQDD AAAVLYSSGT SGRSKGVVVT HRNLIAMVEL FVRFEASQYT 250 260 270 280 290 300 RPARDNVYLA ALPMFHVYGL SLFAVGLLSL GCTVVVMRRF NVDDAVKAIR KYKVTHLPLV 310 320 330 340 350 360 PPIMSALLRA NPPLELDSLL QVSSGAAPLN HTLIHHFLHA FPHVDFIQGY GMTESTAVGT 370 380 390 400 410 420 RGFNTCKHKK YASVGLLAPN MHAKIVHLES GSCLPPGSYG ELWLHGPAIM KEFCFVTGYL 430 440 450 460 470 480 NDDDDAFTRK DGWLRTGDIA YFDSDGYLFI VGRLKDTIKY KGFQIAPADL EAVLIRHPEI 490 500 510 520 530 540 IDVAVTSDED EEAGEIPVAF VVRKSGSTLS CTHVMEYVAK QVRTRRSYTT QHSPYFLAWE 550 560 570 580 HYKIIITASA QSAFLLARVS NSSKNGSLQA LCPPVQINMA DIS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"In-depth view of structure, activity, and evolution of rice chromosome 10." Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H. , Paradkar M., Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F., Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M., Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R., Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F., Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D., Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R., Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K., Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S., Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S., Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R., Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M., Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R., Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E., Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A. Science 300:1566-1569(2003) [PubMed: 12791992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Genome-wide analysis of a land plant-specific acyl:coenzyme A synthetase (ACS) gene family in Arabidopsis, poplar, rice and Physcomitrella." de Azevedo Souza C., Barbazuk B., Ralph S.G., Bohlmann J., Hamberger B., Douglas C.J. New Phytol. 179:987-1003(2008) [PubMed: 18627494] [Abstract] Cited for: GENE FAMILY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC018727 Genomic DNA. Translation: AAG46175.1. Sequence problems. DP000086 Genomic DNA. Translation: ABB48038.2. Sequence problems. CM000147 Genomic DNA. Translation: EAZ17097.1. Sequence problems.
RefSeq	NP_001176290.1. NM_001189361.1.
UniGene	Os.99335.
3D structure databases
HSSP	HSSP built from PDB template 2D1Q based on UniProtKB P13129.
ProteinModelPortal	Q336M7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9272068.
KEGG	osa:9272068.
NMPDR	fig|39947.1.peg.14373.
Organism-specific databases
Gramene	Q9FRQ0.
Phylogenomic databases
GeneTree	EPGT00070000028073.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CLL2_ORYSJ

Accession

Primary (citable) accession number: Q336M7
Secondary accession number(s): A3C7N4, Q9FRQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	October 14, 2008
Last modified:	November 16, 2011
This is version 32 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program