Q336M2 (CDKE1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase E-1
Short name=CDKE;1
EC=2.7.11.22
EC=2.7.11.23

Gene names

Name:	CDKE-1
Ordered Locus Names:	Os10g0580300, LOC_Os10g42950
ORF Names:	OSJNBa0056G17.11

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Induction	By auxin. Down-regulated by cytokinin. Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	Cyclin-dependent kinase E-1		PRO_0000296104
Regions
	Domain	19 – 337	319	Protein kinase
	Nucleotide binding	25 – 33	9	ATP By similarity
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	59	1	ATP By similarity
Amino acid modifications
	Modified residue	29	1	Phosphothreonine By similarity
	Modified residue	30	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q336M2 [UniParc]. Last modified August 22, 2006. Version 2. Checksum: D7CD5C959C39C349	FASTA	475	52,808
	10 20 30 40 50 60 MGDGRVGGGT NRPAWLQQYE LVGKIGEGTY GLVFLARLKQ SHPHAAAGVG RRGSPIAIKK 70 80 90 100 110 120 FKQSKEGDGV SPTAIREIML LREINHENVV KLVNVHINHA DMSLYLAFDY AEHDLYEIIR 130 140 150 160 170 180 HHREKLNLPI NPYTVKSLLW QLLNGLNYLH SNWIIHRDLK PSNILVMGEG EEHGIIKIAD 190 200 210 220 230 240 FGLARIYQAP LKPLSDNGVV VTIWYRAPEL LLGAKHYTSA VDMWAVGCIF AELLTLKPLF 250 260 270 280 290 300 QGVEAKATPN PFQLDQLDKI FKVLGHPTVE KWPTLANLPC WQNDQQHIQG HKYENTGLHN 310 320 330 340 350 360 IVHLPQKSPA FDLLSKMLEY DPRKRITAAQ ALEHEYFRMD PLPGRNALLP SQAGEKIVQY 370 380 390 400 410 420 PVRPVDTTTD FEGTTSLQPT QAPSGNAAPG NQSVVPRPIP RQMQQPMVGM SRMGGTNMAA 430 440 450 460 470 FGAAPQGGIA GMNPGNIPMQ RGAGAQSHPH QLRRKADQGM GMQNPGYPTQ QKRRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"In-depth view of structure, activity, and evolution of rice chromosome 10." Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H. , Paradkar M., Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F., Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M., Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R., Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F., Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D., Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R., Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K., Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S., Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S., Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R., Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M., Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R., Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E., Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A. Science 300:1566-1569(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"Genome-wide identification and expression analysis of rice cell cycle genes." Guo J., Song J., Wang F., Zhang X.S. Plant Mol. Biol. 64:349-360(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, GENE FAMILY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC018727 Genomic DNA. Translation: AAG46164.1. DP000086 Genomic DNA. Translation: ABB48043.2. AP008216 Genomic DNA. Translation: BAF27349.1. AK066824 mRNA. No translation available.
RefSeq	NP_001065512.1. NM_001072044.1.
UniGene	Os.2989.
3D structure databases
HSSP	HSSP built from PDB template 2BTR based on UniProtKB P24941.
ProteinModelPortal	Q336M2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os10g42950.1; LOC_Os10g42950.1; LOC_Os10g42950.
GeneID	4349519.
KEGG	dosa:Os10t0580300-01. osa:4349519.
Organism-specific databases
Gramene	Q336M2.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
KO	K02208.
OMA	ADMSLYL.
ProtClustDB	CLSN2687570.
Gene expression databases
ArrayExpress	Q336M2.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDKE1_ORYSJ

Accession

Primary (citable) accession number: Q336M2
Secondary accession number(s): Q9FRN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	August 22, 2006
Last modified:	May 1, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program