Reviewed, UniProtKB/Swiss-Prot Q32QL6 (GPX4_CALJA)

Names and origin

Protein names

Recommended name:
    Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
      Short name=PHGPx
    EC=1.11.1.12
Alternative name(s):
    Glutathione peroxidase 4
      Short name=GSHPx-4
      Short name=GPx-4

Gene names

Name:

GPX4

Organism

Callithrix jacchus (Common marmoset)

Taxonomic identifier

9483 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Platyrrhini › Cebidae › Callitrichinae › Callithrix

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage By similarity.
Catalytic activity	2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H2O.
Subunit structure	Monomer By similarity.
Subcellular location	Mitochondrion By similarity. Cytoplasm By similarity.
Tissue specificity	Expressed in testis. Also expressed in liver, lung, kidney and spinal cord. Ref.1
Sequence similarities	Belongs to the glutathione peroxidase family.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion
Coding sequence diversity	Alternative initiation Selenocysteine
Domain	Transit peptide
Ligand	Selenium
Molecular function	Developmental protein Oxidoreductase Peroxidase
PTM	Acetylation
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from electronic annotation. Source: InterPro
Molecular function	phospholipid-hydroperoxide glutathione peroxidase activity Inferred from electronic annotation. Source: EC selenium binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Mitochondrial (identifier: Q32QL6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Cytoplasmic (identifier: Q32QL6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 197		Phospholipid hydroperoxide glutathione peroxidase, mitochondrial		PRO_0000318641
Sites
	Active site	73	1	By similarity
Amino acid modifications
	Non-standard residue	73	1	Selenocysteine
	Modified residue	47	1	N6-acetyllysine By similarity
Natural variations
	Alternative sequence	1 – 27	27	Missing in isoform Cytoplasmic.		VSP_031257

Sequences

	Sequence		Length	Mass (Da)
	Isoform Mitochondrial [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 76B48360040EF055	FASTA	197	22,054
	10 20 30 40 50 60 MSLGRLCRLL KPALLCGALA APGLAGTMCA SQDDWRSARS MHEFSAKDID GHTVNLDKYR 70 80 90 100 110 120 GFVCIVTNVA SQUGKTQVNY TQLVDLHARY AECGLRILAF PCNQFGKQEP GSNEEIKEFA 130 140 150 160 170 180 AGYNVKFDMF SKICVNGDDA HPLWKWMKIQ PKGKGTLGNA IKWNFTKFLV DKNGCVVKRY 190 GPMEEPQVIE KDLPCYF
	Isoform Cytoplasmic. Checksum: DBAD3422D1FFDA3F	FASTA	170	19,405
	10 20 30 40 50 60 MCASQDDWRS ARSMHEFSAK DIDGHTVNLD KYRGFVCIVT NVASQUGKTQ VNYTQLVDLH 70 80 90 100 110 120 ARYAECGLRI LAFPCNQFGK QEPGSNEEIK EFAAGYNVKF DMFSKICVNG DDAHPLWKWM 130 140 150 160 170 KIQPKGKGTL GNAIKWNFTK FLVDKNGCVV KRYGPMEEPQ VIEKDLPCYF

References

[1]

"Marmoset glutathione peroxidases: cDNA sequences, molecular evolution, and gene expression." Atanasova S., von Ahsen N., Schlumbohm C., Wieland E., Oellerich M., Armstrong V. J. Med. Primatol. 35:155-164(2006) [PubMed: 16764674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY966404 mRNA. Translation: AAY33855.1.
3D structure databases
HSSP	HSSP built from PDB template 2F8A based on UniProtKB P07203.
ModBase	Search...
Genome annotation databases
Ensembl	ENSCJAT00000014988; ENSCJAP00000014223; ENSCJAG00000007680; Callithrix jacchus. [Genome view]
Phylogenomic databases
HOVERGEN	Q32QL6.
Enzyme and pathway databases
BRENDA	1.11.1.12. 265156.
Family and domain databases
InterPro	IPR000889. Glutathione_peroxidase. IPR012336. Thioredoxin-like_fold. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11592. Glut_peroxidase. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPX4_CALJA

Accession

Primary (citable) accession number: Q32QL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 26, 2008
Last modified:	February 9, 2010
This is version 31 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)