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Q32Q05

- OTU1_RAT

UniProt

Q32Q05 - OTU1_RAT

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Protein
Ubiquitin thioesterase OTU1
Gene
Yod1, Hshin7
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei152 – 1521 By similarity
Active sitei155 – 1551Nucleophile By similarity
Binding sitei261 – 2611Substrate; via carbonyl oxygen By similarity
Active sitei262 – 2621 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri313 – 33725C2H2-type
Add
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ubiquitin-specific protease activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
  2. cellular amino acid metabolic process Source: UniProtKB
  3. endoplasmic reticulum unfolded protein response Source: UniProtKB
  4. protein K11-linked deubiquitination Source: UniProtKB
  5. protein K27-linked deubiquitination Source: UniProtKB
  6. protein K29-linked deubiquitination Source: UniProtKB
  7. protein K33-linked deubiquitination Source: UniProtKB
  8. protein K48-linked deubiquitination Source: UniProtKB
  9. protein K63-linked deubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway, Unfolded protein response

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC85.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase OTU1 (EC:3.4.19.12)
Gene namesi
Name:Yod1
Synonyms:Hshin7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 13

Organism-specific databases

RGDi1359726. Yod1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343Ubiquitin thioesterase OTU1
PRO_0000282358Add
BLAST

Proteomic databases

PRIDEiQ32Q05.

PTM databases

PhosphoSiteiQ32Q05.

Expressioni

Gene expression databases

GenevestigatoriQ32Q05.

Interactioni

Subunit structurei

Interacts with VCP; the interaction is direct. Interacts with FAF2/UBXD8. Interacts with DERL1; however interaction is dependent on the UBAX-like region, suggesting that it may be indirect By similarity.

Protein-protein interaction databases

BioGridi264526. 1 interaction.
STRINGi10116.ENSRNOP00000031836.

Structurei

3D structure databases

ProteinModelPortaliQ32Q05.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini144 – 269126OTU
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni45 – 12379UBX-like
Add
BLAST
Regioni149 – 1557Cys-loop By similarity
Regioni208 – 21811Variable-loop By similarity
Add
BLAST
Regioni258 – 2625His-loop By similarity
Regioni286 – 2916S2 site By similarity

Domaini

The UBAX-like region mediates the interaction with VCP By similarity.
The C2H2-type zinc finger mediates specificity for 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains but not for 'Lys-11'-linked ubiquitin chains. Selectivity for 'Lys-11'-linked ubiquitin chains is provided by recognition of the sequence surrounding 'Lys-11' in ubiquitin. The S2 site region provides specificity for longer 'Lys-11'-linked ubiquitin chains By similarity.

Sequence similaritiesi

Contains 1 OTU domain.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5539.
GeneTreeiENSGT00390000009989.
HOGENOMiHOG000193461.
HOVERGENiHBG097006.
InParanoidiQ32Q05.
KOiK13719.
OMAiKSSRQFT.
OrthoDBiEOG7J1808.
PhylomeDBiQ32Q05.
TreeFamiTF323700.

Family and domain databases

InterProiIPR003323. OTU.
IPR029071. Ubiquitin-rel_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q32Q05-1 [UniParc]FASTAAdd to Basket

« Hide

MFGGAKGGHF GVPPAGCSGA VSQAAAGTKA GPAGGRPADT MWRLRCKAKG    50
GTHVLQGLSN RTRLRELQGQ IAAITGIAPG SQRILVGYPP ECLDLSDRDI 100
TLGDLPIQSG DMLIVEEDQT RPKASPAFSK HGAPSYVRET LPVLTRTAVP 150
ADNSCLFTSV YYVVEGGVLN PACAPEMRRL IAQIVASDPD LYSEAILGKT 200
NEEYCDWIRR DDTWGGAIEI SILSKFYQCE ICVVDTQTVR IDRFGEDAGY 250
TKRVLLIYDG IHYDPLQRNF PDPDTPPLTI FSSNDDIVLV QALELADEAR 300
RKRQFTDVNR FTLRCMLCQK GLTGQAEARD HARETGHTNF GEV 343
Length:343
Mass (Da):37,402
Last modified:December 6, 2005 - v1
Checksum:iAD23FFBF8A4AC502
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC107904 mRNA. Translation: AAI07905.1.
BN000319 mRNA. Translation: CAE48374.1.
RefSeqiNP_001008889.1. NM_001008889.1.
UniGeneiRn.212904.

Genome annotation databases

EnsembliENSRNOT00000030476; ENSRNOP00000031836; ENSRNOG00000025704.
GeneIDi363982.
KEGGirno:363982.
UCSCiRGD:1359726. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC107904 mRNA. Translation: AAI07905.1 .
BN000319 mRNA. Translation: CAE48374.1 .
RefSeqi NP_001008889.1. NM_001008889.1.
UniGenei Rn.212904.

3D structure databases

ProteinModelPortali Q32Q05.
ModBasei Search...

Protein-protein interaction databases

BioGridi 264526. 1 interaction.
STRINGi 10116.ENSRNOP00000031836.

Protein family/group databases

MEROPSi C85.007.

PTM databases

PhosphoSitei Q32Q05.

Proteomic databases

PRIDEi Q32Q05.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000030476 ; ENSRNOP00000031836 ; ENSRNOG00000025704 .
GeneIDi 363982.
KEGGi rno:363982.
UCSCi RGD:1359726. rat.

Organism-specific databases

CTDi 55432.
RGDi 1359726. Yod1.

Phylogenomic databases

eggNOGi COG5539.
GeneTreei ENSGT00390000009989.
HOGENOMi HOG000193461.
HOVERGENi HBG097006.
InParanoidi Q32Q05.
KOi K13719.
OMAi KSSRQFT.
OrthoDBi EOG7J1808.
PhylomeDBi Q32Q05.
TreeFami TF323700.

Miscellaneous databases

NextBioi 684590.
PROi Q32Q05.

Gene expression databases

Genevestigatori Q32Q05.

Family and domain databases

InterProi IPR003323. OTU.
IPR029071. Ubiquitin-rel_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view ]
Pfami PF02338. OTU. 1 hit.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
PROSITEi PS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "A genomic analysis of rat proteases and protease inhibitors."
    Puente X.S., Lopez-Otin C.
    Genome Res. 14:609-622(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiOTU1_RAT
AccessioniPrimary (citable) accession number: Q32Q05
Secondary accession number(s): Q6IE74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: December 6, 2005
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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