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Q32Q05

- OTU1_RAT

UniProt

Q32Q05 - OTU1_RAT

Protein

Ubiquitin thioesterase OTU1

Gene

Yod1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei152 – 1521By similarity
    Active sitei155 – 1551NucleophileBy similarity
    Binding sitei261 – 2611Substrate; via carbonyl oxygenBy similarity
    Active sitei262 – 2621By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri313 – 33725C2H2-typeAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ubiquitin-specific protease activity Source: UniProtKB

    GO - Biological processi

    1. cellular amino acid metabolic process Source: UniProtKB
    2. endoplasmic reticulum unfolded protein response Source: UniProtKB
    3. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
    4. protein K11-linked deubiquitination Source: UniProtKB
    5. protein K27-linked deubiquitination Source: UniProtKB
    6. protein K29-linked deubiquitination Source: UniProtKB
    7. protein K33-linked deubiquitination Source: UniProtKB
    8. protein K48-linked deubiquitination Source: UniProtKB
    9. protein K63-linked deubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway, Unfolded protein response

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC85.007.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin thioesterase OTU1 (EC:3.4.19.12)
    Gene namesi
    Name:Yod1
    Synonyms:Hshin7
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 13

    Organism-specific databases

    RGDi1359726. Yod1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 343343Ubiquitin thioesterase OTU1PRO_0000282358Add
    BLAST

    Proteomic databases

    PRIDEiQ32Q05.

    PTM databases

    PhosphoSiteiQ32Q05.

    Expressioni

    Gene expression databases

    GenevestigatoriQ32Q05.

    Interactioni

    Subunit structurei

    Interacts with VCP; the interaction is direct. Interacts with FAF2/UBXD8. Interacts with DERL1; however interaction is dependent on the UBAX-like region, suggesting that it may be indirect By similarity.By similarity

    Protein-protein interaction databases

    BioGridi264526. 1 interaction.
    STRINGi10116.ENSRNOP00000031836.

    Structurei

    3D structure databases

    ProteinModelPortaliQ32Q05.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini144 – 269126OTUPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni45 – 12379UBX-likeAdd
    BLAST
    Regioni149 – 1557Cys-loopBy similarity
    Regioni208 – 21811Variable-loopBy similarityAdd
    BLAST
    Regioni258 – 2625His-loopBy similarity
    Regioni286 – 2916S2 siteBy similarity

    Domaini

    The UBAX-like region mediates the interaction with VCP.By similarity
    The C2H2-type zinc finger mediates specificity for 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains but not for 'Lys-11'-linked ubiquitin chains. Selectivity for 'Lys-11'-linked ubiquitin chains is provided by recognition of the sequence surrounding 'Lys-11' in ubiquitin. The S2 site region provides specificity for longer 'Lys-11'-linked ubiquitin chains By similarity.By similarity

    Sequence similaritiesi

    Contains 1 C2H2-type zinc finger.Curated
    Contains 1 OTU domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri313 – 33725C2H2-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5539.
    GeneTreeiENSGT00390000009989.
    HOGENOMiHOG000193461.
    HOVERGENiHBG097006.
    InParanoidiQ32Q05.
    KOiK13719.
    OMAiKSSRQFT.
    OrthoDBiEOG7J1808.
    PhylomeDBiQ32Q05.
    TreeFamiTF323700.

    Family and domain databases

    InterProiIPR003323. OTU.
    IPR029071. Ubiquitin-rel_dom.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PfamiPF02338. OTU. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS50802. OTU. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q32Q05-1 [UniParc]FASTAAdd to Basket

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    MFGGAKGGHF GVPPAGCSGA VSQAAAGTKA GPAGGRPADT MWRLRCKAKG    50
    GTHVLQGLSN RTRLRELQGQ IAAITGIAPG SQRILVGYPP ECLDLSDRDI 100
    TLGDLPIQSG DMLIVEEDQT RPKASPAFSK HGAPSYVRET LPVLTRTAVP 150
    ADNSCLFTSV YYVVEGGVLN PACAPEMRRL IAQIVASDPD LYSEAILGKT 200
    NEEYCDWIRR DDTWGGAIEI SILSKFYQCE ICVVDTQTVR IDRFGEDAGY 250
    TKRVLLIYDG IHYDPLQRNF PDPDTPPLTI FSSNDDIVLV QALELADEAR 300
    RKRQFTDVNR FTLRCMLCQK GLTGQAEARD HARETGHTNF GEV 343
    Length:343
    Mass (Da):37,402
    Last modified:December 6, 2005 - v1
    Checksum:iAD23FFBF8A4AC502
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC107904 mRNA. Translation: AAI07905.1.
    BN000319 mRNA. Translation: CAE48374.1.
    RefSeqiNP_001008889.1. NM_001008889.1.
    UniGeneiRn.212904.

    Genome annotation databases

    EnsembliENSRNOT00000030476; ENSRNOP00000031836; ENSRNOG00000025704.
    GeneIDi363982.
    KEGGirno:363982.
    UCSCiRGD:1359726. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC107904 mRNA. Translation: AAI07905.1 .
    BN000319 mRNA. Translation: CAE48374.1 .
    RefSeqi NP_001008889.1. NM_001008889.1.
    UniGenei Rn.212904.

    3D structure databases

    ProteinModelPortali Q32Q05.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 264526. 1 interaction.
    STRINGi 10116.ENSRNOP00000031836.

    Protein family/group databases

    MEROPSi C85.007.

    PTM databases

    PhosphoSitei Q32Q05.

    Proteomic databases

    PRIDEi Q32Q05.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000030476 ; ENSRNOP00000031836 ; ENSRNOG00000025704 .
    GeneIDi 363982.
    KEGGi rno:363982.
    UCSCi RGD:1359726. rat.

    Organism-specific databases

    CTDi 55432.
    RGDi 1359726. Yod1.

    Phylogenomic databases

    eggNOGi COG5539.
    GeneTreei ENSGT00390000009989.
    HOGENOMi HOG000193461.
    HOVERGENi HBG097006.
    InParanoidi Q32Q05.
    KOi K13719.
    OMAi KSSRQFT.
    OrthoDBi EOG7J1808.
    PhylomeDBi Q32Q05.
    TreeFami TF323700.

    Miscellaneous databases

    NextBioi 684590.
    PROi Q32Q05.

    Gene expression databases

    Genevestigatori Q32Q05.

    Family and domain databases

    InterProi IPR003323. OTU.
    IPR029071. Ubiquitin-rel_dom.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    Pfami PF02338. OTU. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS50802. OTU. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    2. "A genomic analysis of rat proteases and protease inhibitors."
      Puente X.S., Lopez-Otin C.
      Genome Res. 14:609-622(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.

    Entry informationi

    Entry nameiOTU1_RAT
    AccessioniPrimary (citable) accession number: Q32Q05
    Secondary accession number(s): Q6IE74
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3