Ubiquitin thioesterase OTU1 - Rattus norvegicus (Rat)

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Q32Q05 (OTU1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 53. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ubiquitin thioesterase OTU1
EC=3.4.19.12

Gene names

Name:	Yod1
Synonyms:	Hshin7

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Able to cleave both polyubiquitin and di-ubiquitin By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Interacts with VCP; the interaction is direct. Interacts with FAF2/UBXD8. Interacts with DERL1; however interaction is dependent on the UBAX-like region, suggesting that it may be indirect By similarity.
Domain	The UBAX-like region mediates the interaction with VCP By similarity.
Sequence similarities	Contains 1 C2H2-type zinc finger. Contains 1 OTU domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway Unfolded protein response
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER-associated protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB cellular amino acid metabolic process Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum unfolded protein response Inferred from sequence or structural similarity. Source: UniProtKB protein K48-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 343

343

Ubiquitin thioesterase OTU1

PRO_0000282358

Regions

Domain

144 – 269

126

OTU

Zinc finger

313 – 337

C2H2-type

Region

45 – 123

UBX-like

Sites

Active site

152

By similarity

Active site

155

Nucleophile By similarity

Active site

337

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q32Q05 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: AD23FFBF8A4AC502
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FASTA

343

37,402

        10         20         30         40         50         60 
MFGGAKGGHF GVPPAGCSGA VSQAAAGTKA GPAGGRPADT MWRLRCKAKG GTHVLQGLSN 

        70         80         90        100        110        120 
RTRLRELQGQ IAAITGIAPG SQRILVGYPP ECLDLSDRDI TLGDLPIQSG DMLIVEEDQT 

       130        140        150        160        170        180 
RPKASPAFSK HGAPSYVRET LPVLTRTAVP ADNSCLFTSV YYVVEGGVLN PACAPEMRRL 

       190        200        210        220        230        240 
IAQIVASDPD LYSEAILGKT NEEYCDWIRR DDTWGGAIEI SILSKFYQCE ICVVDTQTVR 

       250        260        270        280        290        300 
IDRFGEDAGY TKRVLLIYDG IHYDPLQRNF PDPDTPPLTI FSSNDDIVLV QALELADEAR 

       310        320        330        340 
RKRQFTDVNR FTLRCMLCQK GLTGQAEARD HARETGHTNF GEV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[2]	"A genomic analysis of rat proteases and protease inhibitors." Puente X.S., Lopez-Otin C. Genome Res. 14:609-622(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC107904 mRNA. Translation: AAI07905.1. BN000319 mRNA. Translation: CAE48374.1.
IPI	IPI00391965.
RefSeq	NP_001008889.1. NM_001008889.1.
UniGene	Rn.212904.
3D structure databases
ProteinModelPortal	Q32Q05.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000031836.
Protein family/group databases
MEROPS	C85.007.
PTM databases
PhosphoSite	Q32Q05.
Proteomic databases
PRIDE	Q32Q05.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000030476; ENSRNOP00000031836; ENSRNOG00000025704.
GeneID	363982.
KEGG	rno:363982.
UCSC	RGD:1359726. rat.
Organism-specific databases
CTD	55432.
RGD	1359726. Yod1.
Phylogenomic databases
eggNOG	COG5539.
GeneTree	ENSGT00390000009989.
HOGENOM	HOG000193461.
HOVERGEN	HBG097006.
InParanoid	Q32Q05.
KO	K13719.
OMA	PIQSGDM.
OrthoDB	EOG4GF3FR.
Gene expression databases
Genevestigator	Q32Q05.
Family and domain databases
InterPro	IPR003323. OTU. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view]
Pfam	PF02338. OTU. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 1 hit. [Graphical view]
PROSITE	PS50802. OTU. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	684590.

Entry information

Entry name

OTU1_RAT

Accession

Primary (citable) accession number: Q32Q05
Secondary accession number(s): Q6IE74

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	December 6, 2005
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents