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Protein

Small RNA 2'-O-methyltransferase

Gene

Henmt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis.By similarity

Catalytic activityi

S-adenosyl-L-methionine + small RNA = S-adenosyl-L-homocysteine + small RNA containing a 3'-terminal 2'-O-methylnucleotide.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78S-adenosyl-L-methionineBy similarity1
Binding sitei114S-adenosyl-L-methionineBy similarity1
Metal bindingi132MagnesiumBy similarity1
Metal bindingi135MagnesiumBy similarity1
Metal bindingi136Magnesium; via tele nitrogenBy similarity1
Metal bindingi181Magnesium; via tele nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processRNA-mediated gene silencing
LigandMetal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Small RNA 2'-O-methyltransferase (EC:2.1.1.n8By similarity)
Alternative name(s):
HEN1 methyltransferase homolog 1
Gene namesi
Name:Henmt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1306230. Henmt1.

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003041421 – 394Small RNA 2'-O-methyltransferaseAdd BLAST394

Proteomic databases

PaxDbiQ32PY6.
PRIDEiQ32PY6.

Expressioni

Gene expression databases

BgeeiENSRNOG00000042814.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000060946.

Structurei

3D structure databases

ProteinModelPortaliQ32PY6.
SMRiQ32PY6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1045. Eukaryota.
ENOG410XSD6. LUCA.
HOVERGENiHBG097349.
InParanoidiQ32PY6.
KOiK20798.
PhylomeDBiQ32PY6.

Family and domain databases

InterProiView protein in InterPro
IPR026610. Hen1.
IPR029063. SAM-dependent_MTases.
PANTHERiPTHR21404. PTHR21404. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q32PY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METAENIPCS MLGDNFKEVC AEKVIRFRPP LYKQRYQFVR DLVDRHEPKK
60 70 80 90 100
VADLGCGDTK LLKLLKIYPC IQLLVGVDIN EEKLHSNGHR LSPYLGEFVK
110 120 130 140 150
PRDLDLTVTL YHGSVVERDS RLLGFDLITC IELIEHLDSD DLARFPEVVF
160 170 180 190 200
GYLSPAMVVI STPNAEFNPL FPTVTLRDAD HKFEWSRMEF QTWASQVANC
210 220 230 240 250
YNYCVEFTGV GTPPAGSEHV GYCTQIGVFR KNGGKLSEPS ASQQRDQHVY
260 270 280 290 300
KAVYTTSYPS LQQEKVLKFV LVGELLIQVD RLRLRHQRML REQEKERGPK
310 320 330 340 350
PWYTDSSPAP HLLLGAVFTE AEKARIENSP KPFCEGEKFY IPLQRLLTYP
360 370 380 390
KLHRLCADED RMRSLIADSV CLSSDGSAVV VDLHNSWDYR PEDN
Length:394
Mass (Da):45,087
Last modified:December 6, 2005 - v1
Checksum:i49926C2929EB43FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC107926 mRNA. Translation: AAI07927.1.
RefSeqiNP_001032744.2. NM_001037655.1.
UniGeneiRn.47322.

Genome annotation databases

GeneIDi100363095.
KEGGirno:100363095.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiHENMT_RAT
AccessioniPrimary (citable) accession number: Q32PY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: December 6, 2005
Last modified: May 10, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families