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Protein

Small RNA 2'-O-methyltransferase

Gene

Henmt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + small RNA = S-adenosyl-L-homocysteine + small RNA containing a 3'-terminal 2'-O-methylnucleotide.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78S-adenosyl-L-methionineBy similarity1
Binding sitei114S-adenosyl-L-methionineBy similarity1
Metal bindingi132MagnesiumBy similarity1
Metal bindingi135MagnesiumBy similarity1
Metal bindingi136Magnesium; via tele nitrogenBy similarity1
Metal bindingi181Magnesium; via tele nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

RNA-mediated gene silencing

Keywords - Ligandi

Metal-binding, RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Small RNA 2'-O-methyltransferase (EC:2.1.1.n8)
Alternative name(s):
HEN1 methyltransferase homolog 1
Gene namesi
Name:Henmt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1306230. Henmt1.

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003041421 – 394Small RNA 2'-O-methyltransferaseAdd BLAST394

Proteomic databases

PaxDbiQ32PY6.
PRIDEiQ32PY6.

Expressioni

Gene expression databases

BgeeiENSRNOG00000042814.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000060946.

Structurei

3D structure databases

ProteinModelPortaliQ32PY6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1045. Eukaryota.
ENOG410XSD6. LUCA.
HOVERGENiHBG097349.
InParanoidiQ32PY6.
KOiK20798.
PhylomeDBiQ32PY6.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR026610. Hen1.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR21404. PTHR21404. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q32PY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METAENIPCS MLGDNFKEVC AEKVIRFRPP LYKQRYQFVR DLVDRHEPKK
60 70 80 90 100
VADLGCGDTK LLKLLKIYPC IQLLVGVDIN EEKLHSNGHR LSPYLGEFVK
110 120 130 140 150
PRDLDLTVTL YHGSVVERDS RLLGFDLITC IELIEHLDSD DLARFPEVVF
160 170 180 190 200
GYLSPAMVVI STPNAEFNPL FPTVTLRDAD HKFEWSRMEF QTWASQVANC
210 220 230 240 250
YNYCVEFTGV GTPPAGSEHV GYCTQIGVFR KNGGKLSEPS ASQQRDQHVY
260 270 280 290 300
KAVYTTSYPS LQQEKVLKFV LVGELLIQVD RLRLRHQRML REQEKERGPK
310 320 330 340 350
PWYTDSSPAP HLLLGAVFTE AEKARIENSP KPFCEGEKFY IPLQRLLTYP
360 370 380 390
KLHRLCADED RMRSLIADSV CLSSDGSAVV VDLHNSWDYR PEDN
Length:394
Mass (Da):45,087
Last modified:December 6, 2005 - v1
Checksum:i49926C2929EB43FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC107926 mRNA. Translation: AAI07927.1.
RefSeqiNP_001032744.2. NM_001037655.1.
UniGeneiRn.47322.

Genome annotation databases

GeneIDi100363095.
KEGGirno:100363095.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC107926 mRNA. Translation: AAI07927.1.
RefSeqiNP_001032744.2. NM_001037655.1.
UniGeneiRn.47322.

3D structure databases

ProteinModelPortaliQ32PY6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000060946.

Proteomic databases

PaxDbiQ32PY6.
PRIDEiQ32PY6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100363095.
KEGGirno:100363095.

Organism-specific databases

CTDi113802.
RGDi1306230. Henmt1.

Phylogenomic databases

eggNOGiKOG1045. Eukaryota.
ENOG410XSD6. LUCA.
HOVERGENiHBG097349.
InParanoidiQ32PY6.
KOiK20798.
PhylomeDBiQ32PY6.

Miscellaneous databases

PROiQ32PY6.

Gene expression databases

BgeeiENSRNOG00000042814.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR026610. Hen1.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR21404. PTHR21404. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHENMT_RAT
AccessioniPrimary (citable) accession number: Q32PY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: December 6, 2005
Last modified: November 30, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.