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Protein

ATP-citrate synthase

Gene

ACLY

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine (By similarity).By similarity

Catalytic activityi

ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA.

Cofactori

Mg2+By similarity

Enzyme regulationi

Phosphorylation results in a 6-fold increase in V(max) and the conversion of citrate dependence from sigmoidal to hyperbolic. Fructose 6-phosphate (F6P) is also a potent activator.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei58 – 581ATPBy similarity
Binding sitei118 – 1181ATPBy similarity
Metal bindingi201 – 2011MagnesiumBy similarity
Metal bindingi203 – 2031MagnesiumBy similarity
Binding sitei346 – 3461Citrate; via amide nitrogenBy similarity
Binding sitei348 – 3481CitrateBy similarity
Binding sitei379 – 3791CitrateBy similarity
Metal bindingi708 – 7081MagnesiumBy similarity
Active sitei750 – 7501Tele-phosphohistidine intermediateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 672ATPBy similarity
Nucleotide bindingi109 – 1113ATPBy similarity
Nucleotide bindingi691 – 71121ATPBy similarityAdd
BLAST
Nucleotide bindingi742 – 76827ATPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-163765. ChREBP activates metabolic gene expression.
R-BTA-75105. Fatty Acyl-CoA Biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-citrate synthase (EC:2.3.3.8)
Alternative name(s):
ATP-citrate (pro-S-)-lyase
Citrate cleavage enzyme
Gene namesi
Name:ACLY
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10911091ATP-citrate synthasePRO_0000270815Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei131 – 1311PhosphotyrosineBy similarity
Modified residuei263 – 2631PhosphoserineBy similarity
Modified residuei447 – 4471PhosphothreonineBy similarity
Modified residuei451 – 4511PhosphoserineBy similarity
Modified residuei455 – 4551Phosphoserine; by PKA and PKB/AKT1 or PKB/AKT2By similarity
Modified residuei530 – 5301N6-acetyllysine; alternateBy similarity
Cross-linki530 – 530Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei536 – 5361N6-acetyllysine; alternateBy similarity
Cross-linki536 – 536Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei544 – 5441N6-acetyllysine; alternateBy similarity
Cross-linki544 – 544Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei629 – 6291PhosphothreonineBy similarity
Modified residuei653 – 6531PhosphoserineBy similarity
Modified residuei672 – 6721PhosphotyrosineBy similarity
Modified residuei829 – 8291PhosphoserineBy similarity
Modified residuei938 – 9381N6-acetyllysineBy similarity
Modified residuei958 – 9581N6-acetyllysineBy similarity
Modified residuei968 – 9681N6-acetyllysineBy similarity
Modified residuei1067 – 10671N6-acetyllysineBy similarity
Modified residuei1090 – 10901PhosphoserineBy similarity

Post-translational modificationi

ISGylated.By similarity
Acetylated at Lys-530, Lys-536 and Lys-544 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2 (By similarity).By similarity
Ubiquitinated at Lys-530, Lys-536 and Lys-544 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ32PF2.
PRIDEiQ32PF2.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022258.

Structurei

3D structure databases

ProteinModelPortaliQ32PF2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 265262ATP-graspAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni769 – 77911CoA-bindingSequence analysisAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.Curated
In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.Curated
Contains 1 ATP-grasp domain.Curated

Phylogenomic databases

eggNOGiKOG1254. Eukaryota.
COG0045. LUCA.
COG0074. LUCA.
COG0372. LUCA.
GeneTreeiENSGT00530000063275.
HOGENOMiHOG000151479.
HOVERGENiHBG003318.
InParanoidiQ32PF2.
KOiK01648.
OMAiEGRVWTM.
OrthoDBiEOG7CCBQ7.
TreeFamiTF300560.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
3.30.470.20. 1 hit.
3.40.50.261. 2 hits.
3.40.50.720. 1 hit.
InterProiIPR014608. ATP-citrate_synthase.
IPR013816. ATP_grasp_subdomain_2.
IPR017440. Cit_synth/succinyl-CoA_lig_AS.
IPR032263. Citrate-bd.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase.
IPR003781. CoA-bd.
IPR005810. CoA_lig_alpha.
IPR005811. CoA_ligase.
IPR016040. NAD(P)-bd_dom.
IPR017866. Succ-CoA_synthase_bsu_CS.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PfamiPF16114. Citrate_bind. 1 hit.
PF00285. Citrate_synt. 1 hit.
PF02629. CoA_binding. 1 hit.
PF00549. Ligase_CoA. 1 hit.
[Graphical view]
PIRSFiPIRSF036511. ATP_citrt_syn. 1 hit.
SMARTiSM00881. CoA_binding. 1 hit.
[Graphical view]
SUPFAMiSSF48256. SSF48256. 2 hits.
SSF51735. SSF51735. 1 hit.
SSF52210. SSF52210. 1 hit.
PROSITEiPS01216. SUCCINYL_COA_LIG_1. 1 hit.
PS00399. SUCCINYL_COA_LIG_2. 1 hit.
PS01217. SUCCINYL_COA_LIG_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q32PF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAKAISEQT GKELLYKYIC TTSAIQNRFK YARVTPDTDW ARLLQDHPWL
60 70 80 90 100
LSQSLVVKPD QLIKRRGKLG LIGVNLTLDG VKSWLKPRLG QEATVGKATG
110 120 130 140 150
FLKNFLIEPF VPHTQEEEFY VCIYATREGD YVLFHHEGGV DVGDVDAKAQ
160 170 180 190 200
KLLVGVDEKL NPEDIKKHLL VHAPEDKKEI LASFISGLFN FYEDLYFTYL
210 220 230 240 250
EINPLVVTKD GVYVLDLAAK VDATADYICK VKWGDIEFPP PFGREAYPEE
260 270 280 290 300
AYIADLDAKS GASLKLTLLN PKGRIWTMVA GGGASVVYSD TICDLGGVNE
310 320 330 340 350
LANYGEYSGA PSEQQTYDYA KTILSLMTRE KHPDGKILII GGSIANFTNV
360 370 380 390 400
AATFKGIVRA IRDYQGPLKE HEVTIFVRRG GPNYQEGLRV MGEVGKTTGI
410 420 430 440 450
PIHVFGTETH MTAIVGMALG HRPIPNQPPT AAHTANFLLN ASGSTSTPAP
460 470 480 490 500
SRTASFSESR TDEVAPAKKA KPAMLQGKSA TLFSRHTKAI VWGMQTRAVQ
510 520 530 540 550
GMLDFDYVCS RDEPSVAAMV YPFTGDHKQK FYWGHKEILI PVFKNMADAM
560 570 580 590 600
KKHPEVDVLI NFASLRSAYD STMETMNYAQ IRTIAIIAEG IPEALTRKLI
610 620 630 640 650
KKADQKGVTI IGPATVGGIK PGCFKIGNTG GMLDNILASK LYRPGSVAYV
660 670 680 690 700
SRSGGMSNEL NNIISRTTDG VYEGVAIGGD RYPGSTFMDH VLRYQDTAGV
710 720 730 740 750
KMIVVLGEIG GTEEYKICRG VTEGRITKPV VCWCIGTCAA MFSSEVQFGH
760 770 780 790 800
AGACANQASE TAVAKNQALK EAGVFVPRSF DELGEIIQSV YEDLVARGVI
810 820 830 840 850
VPAQEVPPPT VPMDYSWARE LGLIRKPASF MTSICDERGQ ELIYAGMPIT
860 870 880 890 900
EVFKEEMGIG GVLGLLWFQK RLPKYSCQFI EMCLMVTADH GPAVSGAHNT
910 920 930 940 950
IICARAGKDL VSSLTSGLLT IGDRFGGALD AAAKMFSKAF DSGIIPMEFV
960 970 980 990 1000
NKMKKEGKLI MGIGHRVKSI NNPDMRVQIL KDYVRQHFPA TPLLDYALEV
1010 1020 1030 1040 1050
EKITTSKKPN LILNVDGLIG VAFVDMLRHC GSFTREEADE YIDIGALNGI
1060 1070 1080 1090
FVLGRSMGFI GHYLDQKRLK QGLYRHPWDD ISYVLPEHMS M
Length:1,091
Mass (Da):119,789
Last modified:December 6, 2005 - v1
Checksum:iCC88C4054C6568D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC108138 mRNA. Translation: AAI08139.1.
RefSeqiNP_001032534.1. NM_001037457.1.
UniGeneiBt.41387.

Genome annotation databases

EnsembliENSBTAT00000022258; ENSBTAP00000022258; ENSBTAG00000016740.
GeneIDi511135.
KEGGibta:511135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC108138 mRNA. Translation: AAI08139.1.
RefSeqiNP_001032534.1. NM_001037457.1.
UniGeneiBt.41387.

3D structure databases

ProteinModelPortaliQ32PF2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022258.

Proteomic databases

PaxDbiQ32PF2.
PRIDEiQ32PF2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000022258; ENSBTAP00000022258; ENSBTAG00000016740.
GeneIDi511135.
KEGGibta:511135.

Organism-specific databases

CTDi47.

Phylogenomic databases

eggNOGiKOG1254. Eukaryota.
COG0045. LUCA.
COG0074. LUCA.
COG0372. LUCA.
GeneTreeiENSGT00530000063275.
HOGENOMiHOG000151479.
HOVERGENiHBG003318.
InParanoidiQ32PF2.
KOiK01648.
OMAiEGRVWTM.
OrthoDBiEOG7CCBQ7.
TreeFamiTF300560.

Enzyme and pathway databases

ReactomeiR-BTA-163765. ChREBP activates metabolic gene expression.
R-BTA-75105. Fatty Acyl-CoA Biosynthesis.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
3.30.470.20. 1 hit.
3.40.50.261. 2 hits.
3.40.50.720. 1 hit.
InterProiIPR014608. ATP-citrate_synthase.
IPR013816. ATP_grasp_subdomain_2.
IPR017440. Cit_synth/succinyl-CoA_lig_AS.
IPR032263. Citrate-bd.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase.
IPR003781. CoA-bd.
IPR005810. CoA_lig_alpha.
IPR005811. CoA_ligase.
IPR016040. NAD(P)-bd_dom.
IPR017866. Succ-CoA_synthase_bsu_CS.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PfamiPF16114. Citrate_bind. 1 hit.
PF00285. Citrate_synt. 1 hit.
PF02629. CoA_binding. 1 hit.
PF00549. Ligase_CoA. 1 hit.
[Graphical view]
PIRSFiPIRSF036511. ATP_citrt_syn. 1 hit.
SMARTiSM00881. CoA_binding. 1 hit.
[Graphical view]
SUPFAMiSSF48256. SSF48256. 2 hits.
SSF51735. SSF51735. 1 hit.
SSF52210. SSF52210. 1 hit.
PROSITEiPS01216. SUCCINYL_COA_LIG_1. 1 hit.
PS00399. SUCCINYL_COA_LIG_2. 1 hit.
PS01217. SUCCINYL_COA_LIG_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Ascending colon.

Entry informationi

Entry nameiACLY_BOVIN
AccessioniPrimary (citable) accession number: Q32PF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: December 6, 2005
Last modified: June 8, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.