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Q32PA5

- UBE2C_BOVIN

UniProt

Q32PA5 - UBE2C_BOVIN

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Protein
Ubiquitin-conjugating enzyme E2 C
Gene
UBE2C, UBCH10
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit By similarity.

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei114 – 1141Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. acid-amino acid ligase activity Source: InterPro
  3. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
  2. cyclin catabolic process Source: Ensembl
  3. exit from mitosis Source: UniProtKB
  4. free ubiquitin chain polymerization Source: UniProtKB
  5. positive regulation of exit from mitosis Source: Ensembl
  6. protein K11-linked ubiquitination Source: UniProtKB
  7. protein K48-linked ubiquitination Source: UniProtKB
  8. ubiquitin-dependent protein catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_203215. Senescence-Associated Secretory Phenotype (SASP).
REACT_204829. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
REACT_205339. APC/C:Cdc20 mediated degradation of Securin.
REACT_208116. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_209611. Phosphorylation of the APC/C.
REACT_212887. Separation of Sister Chromatids.
REACT_213075. Regulation of APC/C activators between G1/S and early anaphase.
REACT_218125. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_218686. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_220866. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_222622. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 C (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein C
Ubiquitin-protein ligase C
Gene namesi
Name:UBE2C
Synonyms:UBCH10
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 13

Subcellular locationi

GO - Cellular componenti

  1. anaphase-promoting complex Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 179178Ubiquitin-conjugating enzyme E2 C
PRO_0000245040Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei3 – 31Phosphoserine By similarity

Post-translational modificationi

Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ32PA5.

Interactioni

Subunit structurei

Component of the APC/C complex By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022264.

Structurei

3D structure databases

ProteinModelPortaliQ32PA5.
SMRiQ32PA5. Positions 30-175.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110854.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiQ32PA5.
KOiK06688.
OMAiAELWDKD.
OrthoDBiEOG7M0NTH.
TreeFamiTF101116.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q32PA5-1 [UniParc]FASTAAdd to Basket

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MASQNRDPVA ASVAAARKGA EPSGGAARGP VGKRLQQELM TLMMSGDKGI    50
SAFPESDNLF KWVGTIHGAA GTVYEDLRYK LSLEFPSGYP YNAPTVKFLT 100
PCYHPNVDTQ GNICLDILKD KWSALYDVRT ILLSIQSLLG EPNIDSPLNT 150
HAAELWKNPT AFKKYLQETY SKQVSSQDP 179
Length:179
Mass (Da):19,608
Last modified:December 6, 2005 - v1
Checksum:i19F9F5F63A3C97A0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC108195 mRNA. Translation: AAI08196.1.
RefSeqiNP_001032526.1. NM_001037449.2.
UniGeneiBt.53123.

Genome annotation databases

EnsembliENSBTAT00000022264; ENSBTAP00000022264; ENSBTAG00000016746.
GeneIDi506962.
KEGGibta:506962.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC108195 mRNA. Translation: AAI08196.1 .
RefSeqi NP_001032526.1. NM_001037449.2.
UniGenei Bt.53123.

3D structure databases

ProteinModelPortali Q32PA5.
SMRi Q32PA5. Positions 30-175.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000022264.

Proteomic databases

PRIDEi Q32PA5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000022264 ; ENSBTAP00000022264 ; ENSBTAG00000016746 .
GeneIDi 506962.
KEGGi bta:506962.

Organism-specific databases

CTDi 11065.

Phylogenomic databases

eggNOGi COG5078.
GeneTreei ENSGT00730000110854.
HOGENOMi HOG000233454.
HOVERGENi HBG063308.
InParanoidi Q32PA5.
KOi K06688.
OMAi AELWDKD.
OrthoDBi EOG7M0NTH.
TreeFami TF101116.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_203215. Senescence-Associated Secretory Phenotype (SASP).
REACT_204829. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
REACT_205339. APC/C:Cdc20 mediated degradation of Securin.
REACT_208116. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_209611. Phosphorylation of the APC/C.
REACT_212887. Separation of Sister Chromatids.
REACT_213075. Regulation of APC/C activators between G1/S and early anaphase.
REACT_218125. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_218686. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_220866. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_222622. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.

Miscellaneous databases

NextBioi 20867824.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiUBE2C_BOVIN
AccessioniPrimary (citable) accession number: Q32PA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: December 6, 2005
Last modified: September 3, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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