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Q32PA5

- UBE2C_BOVIN

UniProt

Q32PA5 - UBE2C_BOVIN

Protein

Ubiquitin-conjugating enzyme E2 C

Gene

UBE2C

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit By similarity.By similarity

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei114 – 1141Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
    2. cyclin catabolic process Source: Ensembl
    3. exit from mitosis Source: UniProtKB
    4. free ubiquitin chain polymerization Source: UniProtKB
    5. positive regulation of exit from mitosis Source: Ensembl
    6. protein K11-linked ubiquitination Source: UniProtKB
    7. protein K48-linked ubiquitination Source: UniProtKB
    8. ubiquitin-dependent protein catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_203215. Senescence-Associated Secretory Phenotype (SASP).
    REACT_204829. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
    REACT_205339. APC/C:Cdc20 mediated degradation of Securin.
    REACT_208116. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_209611. Phosphorylation of the APC/C.
    REACT_212887. Separation of Sister Chromatids.
    REACT_213075. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_218125. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_218686. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_220866. APC/C:Cdc20 mediated degradation of mitotic proteins.
    REACT_222622. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 C (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein C
    Ubiquitin-protein ligase C
    Gene namesi
    Name:UBE2C
    Synonyms:UBCH10
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 13

    Subcellular locationi

    GO - Cellular componenti

    1. anaphase-promoting complex Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 179178Ubiquitin-conjugating enzyme E2 CPRO_0000245040Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei3 – 31PhosphoserineBy similarity

    Post-translational modificationi

    Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiQ32PA5.

    Interactioni

    Subunit structurei

    Component of the APC/C complex.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000022264.

    Structurei

    3D structure databases

    ProteinModelPortaliQ32PA5.
    SMRiQ32PA5. Positions 30-175.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00730000110854.
    HOGENOMiHOG000233454.
    HOVERGENiHBG063308.
    InParanoidiQ32PA5.
    KOiK06688.
    OMAiAELWDKD.
    OrthoDBiEOG7M0NTH.
    TreeFamiTF101116.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q32PA5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASQNRDPVA ASVAAARKGA EPSGGAARGP VGKRLQQELM TLMMSGDKGI    50
    SAFPESDNLF KWVGTIHGAA GTVYEDLRYK LSLEFPSGYP YNAPTVKFLT 100
    PCYHPNVDTQ GNICLDILKD KWSALYDVRT ILLSIQSLLG EPNIDSPLNT 150
    HAAELWKNPT AFKKYLQETY SKQVSSQDP 179
    Length:179
    Mass (Da):19,608
    Last modified:December 6, 2005 - v1
    Checksum:i19F9F5F63A3C97A0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC108195 mRNA. Translation: AAI08196.1.
    RefSeqiNP_001032526.1. NM_001037449.2.
    UniGeneiBt.53123.

    Genome annotation databases

    EnsembliENSBTAT00000022264; ENSBTAP00000022264; ENSBTAG00000016746.
    GeneIDi506962.
    KEGGibta:506962.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC108195 mRNA. Translation: AAI08196.1 .
    RefSeqi NP_001032526.1. NM_001037449.2.
    UniGenei Bt.53123.

    3D structure databases

    ProteinModelPortali Q32PA5.
    SMRi Q32PA5. Positions 30-175.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000022264.

    Proteomic databases

    PRIDEi Q32PA5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000022264 ; ENSBTAP00000022264 ; ENSBTAG00000016746 .
    GeneIDi 506962.
    KEGGi bta:506962.

    Organism-specific databases

    CTDi 11065.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00730000110854.
    HOGENOMi HOG000233454.
    HOVERGENi HBG063308.
    InParanoidi Q32PA5.
    KOi K06688.
    OMAi AELWDKD.
    OrthoDBi EOG7M0NTH.
    TreeFami TF101116.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_203215. Senescence-Associated Secretory Phenotype (SASP).
    REACT_204829. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
    REACT_205339. APC/C:Cdc20 mediated degradation of Securin.
    REACT_208116. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_209611. Phosphorylation of the APC/C.
    REACT_212887. Separation of Sister Chromatids.
    REACT_213075. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_218125. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_218686. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_220866. APC/C:Cdc20 mediated degradation of mitotic proteins.
    REACT_222622. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.

    Miscellaneous databases

    NextBioi 20867824.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Liver.

    Entry informationi

    Entry nameiUBE2C_BOVIN
    AccessioniPrimary (citable) accession number: Q32PA5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3