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Protein

Ubiquitin-conjugating enzyme E2 C

Gene

UBE2C

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.By similarity

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotationBy similarity
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N6-monoubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei114Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-BTA-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-BTA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-BTA-174154 APC/C:Cdc20 mediated degradation of Securin
R-BTA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-BTA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-BTA-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-BTA-176408 Regulation of APC/C activators between G1/S and early anaphase
R-BTA-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-BTA-176412 Phosphorylation of the APC/C
R-BTA-179409 APC-Cdc20 mediated degradation of Nek2A
R-BTA-2467813 Separation of Sister Chromatids
R-BTA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-BTA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-BTA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-BTA-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 C (EC:2.3.2.23)
Alternative name(s):
(E3-independent) E2 ubiquitin-conjugating enzyme C (EC:2.3.2.24)
E2 ubiquitin-conjugating enzyme C
Ubiquitin carrier protein C
Ubiquitin-protein ligase C
Gene namesi
Name:UBE2C
Synonyms:UBCH10
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 13

Organism-specific databases

VGNCiVGNC:36578 UBE2C

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002450402 – 179Ubiquitin-conjugating enzyme E2 CAdd BLAST178

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei3PhosphoserineBy similarity1

Post-translational modificationi

Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ32PA5
PRIDEiQ32PA5

Expressioni

Gene expression databases

BgeeiENSBTAG00000016746

Interactioni

Subunit structurei

Component of the APC/C complex, composed of at least 14 distinct subunits that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa. Within this complex, directly interacts with ANAPC2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022264

Structurei

3D structure databases

ProteinModelPortaliQ32PA5
SMRiQ32PA5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0421 Eukaryota
ENOG4111IGV LUCA
GeneTreeiENSGT00760000119350
HOGENOMiHOG000233454
HOVERGENiHBG063308
InParanoidiQ32PA5
KOiK06688
OMAiAELWDKD
OrthoDBiEOG091G0M7S
TreeFamiTF101116

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q32PA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASQNRDPVA ASVAAARKGA EPSGGAARGP VGKRLQQELM TLMMSGDKGI
60 70 80 90 100
SAFPESDNLF KWVGTIHGAA GTVYEDLRYK LSLEFPSGYP YNAPTVKFLT
110 120 130 140 150
PCYHPNVDTQ GNICLDILKD KWSALYDVRT ILLSIQSLLG EPNIDSPLNT
160 170
HAAELWKNPT AFKKYLQETY SKQVSSQDP
Length:179
Mass (Da):19,608
Last modified:December 6, 2005 - v1
Checksum:i19F9F5F63A3C97A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC108195 mRNA Translation: AAI08196.1
RefSeqiNP_001032526.1, NM_001037449.2
UniGeneiBt.53123

Genome annotation databases

EnsembliENSBTAT00000022264; ENSBTAP00000022264; ENSBTAG00000016746
GeneIDi506962
KEGGibta:506962

Similar proteinsi

Entry informationi

Entry nameiUBE2C_BOVIN
AccessioniPrimary (citable) accession number: Q32PA5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: December 6, 2005
Last modified: May 23, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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