ID   UBE2B_BOVIN             Reviewed;         152 AA.
AC   Q32P99;
DT   13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   27-MAR-2024, entry version 126.
DE   RecName: Full=Ubiquitin-conjugating enzyme E2 B {ECO:0000305};
DE            EC=2.3.2.23 {ECO:0000250|UniProtKB:P63146};
DE   AltName: Full=E2 ubiquitin-conjugating enzyme B;
DE   AltName: Full=Ubiquitin carrier protein B;
DE   AltName: Full=Ubiquitin-protein ligase B;
GN   Name=UBE2B;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Accepts ubiquitin from the E1 complex and catalyzes its
CC       covalent attachment to other proteins. In association with the E3
CC       enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription
CC       regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-
CC       120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic
CC       transcriptional activation, elongation by RNA polymerase II, telomeric
CC       silencing, and is also a prerequisite for H3K4me and H3K79me formation.
CC       In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked
CC       polyubiquitination. Required for postreplication repair of UV-damaged
CC       DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18
CC       ubiquitin ligase complex involved in mono-ubiquitination of DNA-
CC       associated PCNA on 'Lys-164'. May be involved in neurite outgrowth. May
CC       play a role in DNA repair (By similarity).
CC       {ECO:0000250|UniProtKB:P63146}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC         [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC         activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC         conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC         Evidence={ECO:0000250|UniProtKB:P63146, ECO:0000255|PROSITE-
CC         ProRule:PRU00388, ECO:0000255|PROSITE-ProRule:PRU10133};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000250|UniProtKB:P63146, ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- SUBUNIT: Interacts with RAD18, UBR2 and WAC.
CC       {ECO:0000250|UniProtKB:P63146}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P63149}.
CC       Nucleus {ECO:0000250|UniProtKB:P63149}. Note=In peripheral neurons,
CC       expressed both at the plasma membrane and in nuclei.
CC       {ECO:0000250|UniProtKB:P63149}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR   EMBL; BC108202; AAI08203.1; -; mRNA.
DR   RefSeq; NP_001032536.1; NM_001037459.2.
DR   AlphaFoldDB; Q32P99; -.
DR   BMRB; Q32P99; -.
DR   SMR; Q32P99; -.
DR   STRING; 9913.ENSBTAP00000040823; -.
DR   PaxDb; 9913-ENSBTAP00000040823; -.
DR   Ensembl; ENSBTAT00000043235.2; ENSBTAP00000040823.1; ENSBTAG00000010982.6.
DR   GeneID; 512207; -.
DR   KEGG; bta:512207; -.
DR   CTD; 7320; -.
DR   VEuPathDB; HostDB:ENSBTAG00000010982; -.
DR   VGNC; VGNC:36577; UBE2B.
DR   eggNOG; KOG0419; Eukaryota.
DR   GeneTree; ENSGT00940000156580; -.
DR   HOGENOM; CLU_030988_10_2_1; -.
DR   InParanoid; Q32P99; -.
DR   OMA; DNIMCCH; -.
DR   OrthoDB; 5478564at2759; -.
DR   TreeFam; TF101128; -.
DR   Reactome; R-BTA-110314; Recognition of DNA damage by PCNA-containing replication complex.
DR   Reactome; R-BTA-8866652; Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
DR   Reactome; R-BTA-8866654; E3 ubiquitin ligases ubiquitinate target proteins.
DR   Reactome; R-BTA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000009136; Chromosome 7.
DR   Bgee; ENSBTAG00000010982; Expressed in semitendinosus and 103 other cell types or tissues.
DR   GO; GO:0033503; C:HULC complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IBA:GO_Central.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; ISS:UniProtKB.
DR   GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR   GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   GO; GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB.
DR   GO; GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB.
DR   GO; GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB.
DR   GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR   CDD; cd00195; UBCc; 1.
DR   Gene3D; 3.10.110.10; Ubiquitin Conjugating Enzyme; 1.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   InterPro; IPR023313; UBQ-conjugating_AS.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   PANTHER; PTHR24067; UBIQUITIN-CONJUGATING ENZYME E2; 1.
DR   PANTHER; PTHR24067:SF246; UBIQUITIN-CONJUGATING ENZYME E2 B; 1.
DR   Pfam; PF00179; UQ_con; 1.
DR   SMART; SM00212; UBCc; 1.
DR   SUPFAM; SSF54495; UBC-like; 1.
DR   PROSITE; PS00183; UBC_1; 1.
DR   PROSITE; PS50127; UBC_2; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cell membrane; DNA damage; DNA repair; Membrane;
KW   Nucleotide-binding; Nucleus; Reference proteome; Transferase;
KW   Ubl conjugation pathway.
FT   CHAIN           1..152
FT                   /note="Ubiquitin-conjugating enzyme E2 B"
FT                   /id="PRO_0000239456"
FT   DOMAIN          4..150
FT                   /note="UBC core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT   ACT_SITE        88
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT                   ECO:0000255|PROSITE-ProRule:PRU10133"
SQ   SEQUENCE   152 AA;  17312 MW;  CFDEEEE7E06840BE CRC64;
     MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED GTFKLVIEFS
     EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS
     PANSQAAQLY QENKREYEKR VSAIVEQSWN DS
//