Reviewed, UniProtKB/Swiss-Prot Q32P44 (EMAL3_HUMAN)

Names and origin

Protein names

Recommended name:
    Echinoderm microtubule-associated protein-like 3
      Short name=EMAP-3

Gene names

Name:

EML3

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	896 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the WD repeat EMAP family. Contains 9 WD repeats.

Ontologies

Keywords
Cellular component	Cytoplasm Microtubule
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NEK6	Q9HC98	1	EBI-1046713,EBI-740364
YWHAQ	P27348	1	EBI-1046713,EBI-359854

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q32P44-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q32P44-2) The sequence of this isoform differs from the canonical sequence as follows:      786-896: GVWPDGSDGT...SLSPASSLDV → VPVRSCQGAE...GANGAPAHTV
Note: No experimental confirmation available.
Sequence annotation (Features)
	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
	Natural variant	788	1	V → M: dbSNP rs34709729.
Isoform 3 (identifier: Q32P44-3) The sequence of this isoform differs from the canonical sequence as follows:      1-163: MDGAAGPGDG...RNSSSSSSPS → MDPQPLRGPA...PCSPHSVLTQ
Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 896	896	Echinoderm microtubule-associated protein-like 3		PRO_0000284390
Regions
	Repeat	303 – 352	50	WD 1
	Repeat	357 – 400	44	WD 2
	Repeat	403 – 442	40	WD 3
	Repeat	457 – 495	39	WD 4
	Repeat	512 – 551	40	WD 5
	Repeat	595 – 634	40	WD 6
	Repeat	678 – 717	40	WD 7
	Repeat	724 – 763	40	WD 8
	Repeat	837 – 877	41	WD 9
	Compositional bias	126 – 129	4	Poly-Ser
	Compositional bias	156 – 163	8	Poly-Ser
	Compositional bias	287 – 297	11	Poly-Gly
Amino acid modifications
	Modified residue	176	1	Phosphoserine Ref.3 Ref.5 Ref.7
	Modified residue	881	1	Phosphothreonine Ref.3 Ref.5 Ref.4
Natural variations
	Alternative sequence	1 – 163	163	MDGAA…SSSPS → MDPQPLRGPAKSLVGPNLKE GAAAAVVLAPLAPRGSSGPC SPHSVLTQ in isoform 3.		VSP_024482
	Alternative sequence	786 – 896	111	GVWPD…SSLDV → VPVRSCQGAEPHVRGPRQPR DQRPIHARRLAPRLAGRQGR QHLPVASAGRWGRGAGARHA LSNPLPVPRLLPRRLIAAWR DRLARRRGPAPPCPSLAQSP TTRGRLFPGLTSRHSRSRIF LEGANGAPAHTV in isoform 2.		VSP_024483
	Natural variant	620	1	Q → K: dbSNP rs34098002.		VAR_031725
Experimental info
	Sequence conflict	65	1	S → SS in BAC04073. Ref.1
	Sequence conflict	381	1	D → N in BAC04073. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 21D2EBD29B969D34	FASTA	896	95,197
	10 20 30 40 50 60 MDGAAGPGDG PAREALQSLS QRLRVQEQEM ELVKAALAEA LRLLRLQVPP SSLQGSGTPA 70 80 90 100 110 120 PPGDSLAAPP GLPPTCTPSL VSRGTQTETE VELKSSPGPP GLSNGPPAPQ GASEEPSGTQ 130 140 150 160 170 180 SEGGGSSSSG AGSPGPPGIL RPLQPPQRAD TPRRNSSSSS SPSERPRQKL SRKAISSANL 190 200 210 220 230 240 LVRSGSTESR GGKDPLSSPG GPGSRRSNYN LEGISVKMFL RGRPITMYIP SGIRSLEELP 250 260 270 280 290 300 SGPPPETLSL DWVYGYRGRD SRSNLFVLRS GEVVYFIACV VVLYRPGGGP GGPGGGGQRH 310 320 330 340 350 360 YRGHTDCVRC LAVHPDGVRV ASGQTAGVDK DGKPLQPVVH IWDSETLLKL QEIGLGAFER 370 380 390 400 410 420 GVGALAFSAA DQGAFLCVVD DSNEHMLSVW DCSRGMKLAE IKSTNDSVLA VGFNPRDSSC 430 440 450 460 470 480 IVTSGKSHVH FWNWSGGVGV PGNGTLTRKQ GVFGKYKKPK FIPCFVFLPD GDILTGDSEG 490 500 510 520 530 540 NILTWGRSPS DSKTPGRGGA KETYGIVAQA HAHEGSIFAL CLRRDGTVLS GGGRDRRLVQ 550 560 570 580 590 600 WGPGLVALQE AEIPEHFGAV RAIAEGLGSE LLVGTTKNAL LRGDLAQGFS PVIQGHTDEL 610 620 630 640 650 660 WGLCTHPSQN RFLTCGHDRQ LCLWDGESHA LAWSIDLKET GLCADFHPSG AVVAVGLNTG 670 680 690 700 710 720 RWLVLDTETR EIVSDVIDGN EQLSVVRYSP DGLYLAIGSH DNVIYIYSVS SDGAKSSRFG 730 740 750 760 770 780 RCMGHSSFIT HLDWSKDGNF IMSNSGDYEI LYWDVAGGCK QLKNRYESRD REWATYTCVL 790 800 810 820 830 840 GFHVYGVWPD GSDGTDINSL CRSHNERVVA VADDFCKVHL FQYPCARAKA PSRMYGGHGS 850 860 870 880 890 HVTSVRFTHD DSHLVSLGGK DASIFQWRVL GAGGAGPAPA TPSRTPSLSP ASSLDV
	Isoform 2. Checksum: C5E4325DCE7C226F	FASTA	917	97,987
	10 20 30 40 50 60 MDGAAGPGDG PAREALQSLS QRLRVQEQEM ELVKAALAEA LRLLRLQVPP SSLQGSGTPA 70 80 90 100 110 120 PPGDSLAAPP GLPPTCTPSL VSRGTQTETE VELKSSPGPP GLSNGPPAPQ GASEEPSGTQ 130 140 150 160 170 180 SEGGGSSSSG AGSPGPPGIL RPLQPPQRAD TPRRNSSSSS SPSERPRQKL SRKAISSANL 190 200 210 220 230 240 LVRSGSTESR GGKDPLSSPG GPGSRRSNYN LEGISVKMFL RGRPITMYIP SGIRSLEELP 250 260 270 280 290 300 SGPPPETLSL DWVYGYRGRD SRSNLFVLRS GEVVYFIACV VVLYRPGGGP GGPGGGGQRH 310 320 330 340 350 360 YRGHTDCVRC LAVHPDGVRV ASGQTAGVDK DGKPLQPVVH IWDSETLLKL QEIGLGAFER 370 380 390 400 410 420 GVGALAFSAA DQGAFLCVVD DSNEHMLSVW DCSRGMKLAE IKSTNDSVLA VGFNPRDSSC 430 440 450 460 470 480 IVTSGKSHVH FWNWSGGVGV PGNGTLTRKQ GVFGKYKKPK FIPCFVFLPD GDILTGDSEG 490 500 510 520 530 540 NILTWGRSPS DSKTPGRGGA KETYGIVAQA HAHEGSIFAL CLRRDGTVLS GGGRDRRLVQ 550 560 570 580 590 600 WGPGLVALQE AEIPEHFGAV RAIAEGLGSE LLVGTTKNAL LRGDLAQGFS PVIQGHTDEL 610 620 630 640 650 660 WGLCTHPSQN RFLTCGHDRQ LCLWDGESHA LAWSIDLKET GLCADFHPSG AVVAVGLNTG 670 680 690 700 710 720 RWLVLDTETR EIVSDVIDGN EQLSVVRYSP DGLYLAIGSH DNVIYIYSVS SDGAKSSRFG 730 740 750 760 770 780 RCMGHSSFIT HLDWSKDGNF IMSNSGDYEI LYWDVAGGCK QLKNRYESRD REWATYTCVL 790 800 810 820 830 840 GFHVYVPVRS CQGAEPHVRG PRQPRDQRPI HARRLAPRLA GRQGRQHLPV ASAGRWGRGA 850 860 870 880 890 900 GARHALSNPL PVPRLLPRRL IAAWRDRLAR RRGPAPPCPS LAQSPTTRGR LFPGLTSRHS 910 RSRIFLEGAN GAPAHTV
	Isoform 3. Checksum: 38E55507F72F5D15	FASTA	781	83,729
	10 20 30 40 50 60 MDPQPLRGPA KSLVGPNLKE GAAAAVVLAP LAPRGSSGPC SPHSVLTQER PRQKLSRKAI 70 80 90 100 110 120 SSANLLVRSG STESRGGKDP LSSPGGPGSR RSNYNLEGIS VKMFLRGRPI TMYIPSGIRS 130 140 150 160 170 180 LEELPSGPPP ETLSLDWVYG YRGRDSRSNL FVLRSGEVVY FIACVVVLYR PGGGPGGPGG 190 200 210 220 230 240 GGQRHYRGHT DCVRCLAVHP DGVRVASGQT AGVDKDGKPL QPVVHIWDSE TLLKLQEIGL 250 260 270 280 290 300 GAFERGVGAL AFSAADQGAF LCVVDDSNEH MLSVWDCSRG MKLAEIKSTN DSVLAVGFNP 310 320 330 340 350 360 RDSSCIVTSG KSHVHFWNWS GGVGVPGNGT LTRKQGVFGK YKKPKFIPCF VFLPDGDILT 370 380 390 400 410 420 GDSEGNILTW GRSPSDSKTP GRGGAKETYG IVAQAHAHEG SIFALCLRRD GTVLSGGGRD 430 440 450 460 470 480 RRLVQWGPGL VALQEAEIPE HFGAVRAIAE GLGSELLVGT TKNALLRGDL AQGFSPVIQG 490 500 510 520 530 540 HTDELWGLCT HPSQNRFLTC GHDRQLCLWD GESHALAWSI DLKETGLCAD FHPSGAVVAV 550 560 570 580 590 600 GLNTGRWLVL DTETREIVSD VIDGNEQLSV VRYSPDGLYL AIGSHDNVIY IYSVSSDGAK 610 620 630 640 650 660 SSRFGRCMGH SSFITHLDWS KDGNFIMSNS GDYEILYWDV AGGCKQLKNR YESRDREWAT 670 680 690 700 710 720 YTCVLGFHVY GVWPDGSDGT DINSLCRSHN ERVVAVADDF CKVHLFQYPC ARAKAPSRMY 730 740 750 760 770 780 GGHGSHVTSV RFTHDDSHLV SLGGKDASIF QWRVLGAGGA GPAPATPSRT PSLSPASSLD V

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-896 (ISOFORM 2). Tissue: Testis and Uterus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus.
[3]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176 AND THR-881, MASS SPECTROMETRY. Tissue: Epithelium.
[4]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-881, MASS SPECTROMETRY.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176 AND THR-881, MASS SPECTROMETRY.
[6]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, MASS SPECTROMETRY. Tissue: T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK093146 mRNA. Translation: BAC04073.1. Different initiation. AK128679 mRNA. Translation: BAC87566.1. BC108280 mRNA. Translation: AAI08281.1.
IPI	IPI00167909. IPI00843902. IPI00843958.
RefSeq	NP_694997.2.
UniGene	Hs.379785
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q32P44. 3 interactions.
STRING	Q32P44.
PTM databases
PhosphoSite	Q32P44.
Proteomic databases
PeptideAtlas	Q32P44.
PRIDE	Q32P44.
Genome annotation databases
Ensembl	ENST00000394773; ENSP00000378254; ENSG00000149499; Homo sapiens. [Genome view]
GeneID	256364.
KEGG	hsa:256364.
UCSC	uc001ntr.1. human. uc001nts.1. human. uc001ntt.1. human.
Organism-specific databases
CTD	256364.
GeneCards	GC11M062127.
HGNC	HGNC:26666. EML3.
PharmGKB	PA142671910.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q32P44.
OrthoDB	EOG9KD95C.
Gene expression databases
ArrayExpress	Q32P44.
Bgee	Q32P44.
CleanEx	HS_EML3.
Genevestigator	Q32P44.
Family and domain databases
InterPro	IPR005108. HELP. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019782. WD40_repeat_2. IPR017986. WD40_repeat_dom. IPR019781. WD40_repeat_sg. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 2 hits.
Pfam	PF03451. HELP. 1 hit. PF00400. WD40. 5 hits. [Graphical view]
SMART	SM00320. WD40. 8 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. 3 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	92788.

Entry information

Entry name

EMAL3_HUMAN

Accession

Primary (citable) accession number: Q32P44
Secondary accession number(s): Q6ZQW7, Q8NA55

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	December 6, 2005
Last modified:	November 24, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.