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Protein

Echinoderm microtubule-associated protein-like 3

Gene

EML3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.By similarity

Enzyme and pathway databases

SignaLinkiQ32P44.

Names & Taxonomyi

Protein namesi
Recommended name:
Echinoderm microtubule-associated protein-like 3
Short name:
EMAP-3
Gene namesi
Name:EML3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:26666. EML3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671910.

Polymorphism and mutation databases

BioMutaiEML3.
DMDMi121945710.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 896896Echinoderm microtubule-associated protein-like 3PRO_0000284390Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei176 – 1761Phosphoserine5 Publications
Modified residuei881 – 8811Phosphothreonine1 Publication
Modified residuei883 – 8831Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ32P44.
PaxDbiQ32P44.
PeptideAtlasiQ32P44.
PRIDEiQ32P44.

PTM databases

PhosphoSiteiQ32P44.

Expressioni

Gene expression databases

BgeeiQ32P44.
CleanExiHS_EML3.
ExpressionAtlasiQ32P44. baseline and differential.
GenevisibleiQ32P44. HS.

Interactioni

Protein-protein interaction databases

BioGridi129161. 6 interactions.
IntActiQ32P44. 3 interactions.
MINTiMINT-1682086.
STRINGi9606.ENSP00000378254.

Structurei

3D structure databases

ProteinModelPortaliQ32P44.
SMRiQ32P44. Positions 216-870.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati303 – 35250WD 1Add
BLAST
Repeati357 – 40044WD 2Add
BLAST
Repeati403 – 44240WD 3Add
BLAST
Repeati457 – 49539WD 4Add
BLAST
Repeati512 – 55140WD 5Add
BLAST
Repeati595 – 63440WD 6Add
BLAST
Repeati678 – 71740WD 7Add
BLAST
Repeati724 – 76340WD 8Add
BLAST
Repeati837 – 87741WD 9Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi126 – 1294Poly-Ser
Compositional biasi156 – 1638Poly-Ser
Compositional biasi287 – 29711Poly-GlyAdd
BLAST

Sequence similaritiesi

Belongs to the WD repeat EMAP family.Curated
Contains 9 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00550000074369.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ32P44.
KOiK18596.
OMAiXETGLCA.
OrthoDBiEOG7XWPMW.
PhylomeDBiQ32P44.
TreeFamiTF317832.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR005108. HELP.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 3 hits.
PROSITEiPS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q32P44-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGAAGPGDG PAREALQSLS QRLRVQEQEM ELVKAALAEA LRLLRLQVPP
60 70 80 90 100
SSLQGSGTPA PPGDSLAAPP GLPPTCTPSL VSRGTQTETE VELKSSPGPP
110 120 130 140 150
GLSNGPPAPQ GASEEPSGTQ SEGGGSSSSG AGSPGPPGIL RPLQPPQRAD
160 170 180 190 200
TPRRNSSSSS SPSERPRQKL SRKAISSANL LVRSGSTESR GGKDPLSSPG
210 220 230 240 250
GPGSRRSNYN LEGISVKMFL RGRPITMYIP SGIRSLEELP SGPPPETLSL
260 270 280 290 300
DWVYGYRGRD SRSNLFVLRS GEVVYFIACV VVLYRPGGGP GGPGGGGQRH
310 320 330 340 350
YRGHTDCVRC LAVHPDGVRV ASGQTAGVDK DGKPLQPVVH IWDSETLLKL
360 370 380 390 400
QEIGLGAFER GVGALAFSAA DQGAFLCVVD DSNEHMLSVW DCSRGMKLAE
410 420 430 440 450
IKSTNDSVLA VGFNPRDSSC IVTSGKSHVH FWNWSGGVGV PGNGTLTRKQ
460 470 480 490 500
GVFGKYKKPK FIPCFVFLPD GDILTGDSEG NILTWGRSPS DSKTPGRGGA
510 520 530 540 550
KETYGIVAQA HAHEGSIFAL CLRRDGTVLS GGGRDRRLVQ WGPGLVALQE
560 570 580 590 600
AEIPEHFGAV RAIAEGLGSE LLVGTTKNAL LRGDLAQGFS PVIQGHTDEL
610 620 630 640 650
WGLCTHPSQN RFLTCGHDRQ LCLWDGESHA LAWSIDLKET GLCADFHPSG
660 670 680 690 700
AVVAVGLNTG RWLVLDTETR EIVSDVIDGN EQLSVVRYSP DGLYLAIGSH
710 720 730 740 750
DNVIYIYSVS SDGAKSSRFG RCMGHSSFIT HLDWSKDGNF IMSNSGDYEI
760 770 780 790 800
LYWDVAGGCK QLKNRYESRD REWATYTCVL GFHVYGVWPD GSDGTDINSL
810 820 830 840 850
CRSHNERVVA VADDFCKVHL FQYPCARAKA PSRMYGGHGS HVTSVRFTHD
860 870 880 890
DSHLVSLGGK DASIFQWRVL GAGGAGPAPA TPSRTPSLSP ASSLDV
Length:896
Mass (Da):95,197
Last modified:December 6, 2005 - v1
Checksum:i21D2EBD29B969D34
GO
Isoform 2 (identifier: Q32P44-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     786-896: GVWPDGSDGT...SLSPASSLDV → VPVRSCQGAE...GANGAPAHTV

Note: No experimental confirmation available.
Show »
Length:917
Mass (Da):97,987
Checksum:iC5E4325DCE7C226F
GO
Isoform 3 (identifier: Q32P44-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.
     152-223: PRRNSSSSSS...ISVKMFLRGR → AASAEALQEG...HVHPVWHPQP
     224-896: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:194
Mass (Da):20,224
Checksum:iFBA484EF88EBA319
GO

Sequence cautioni

The sequence BAC04073.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC87566.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651S → SS in BAC04073 (PubMed:14702039).Curated
Sequence conflicti381 – 3811D → N in BAC04073 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti620 – 6201Q → K.
Corresponds to variant rs34098002 [ dbSNP | Ensembl ].
VAR_031725
Isoform 2 (identifier: Q32P44-2)
Natural varianti788 – 7881V → M.
Corresponds to variant rs34709729 [ dbSNP | Ensembl ].

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2929Missing in isoform 3. 1 PublicationVSP_039926Add
BLAST
Alternative sequencei152 – 22372PRRNS…FLRGR → AASAEALQEGNLLRQPVSAV REHREPWGKRPPLQPWGPWI SEEQLQFGRHLSEDVPSRAP HYHVHPVWHPQP in isoform 3. 1 PublicationVSP_039927Add
BLAST
Alternative sequencei224 – 896673Missing in isoform 3. 1 PublicationVSP_039928Add
BLAST
Alternative sequencei786 – 896111GVWPD…SSLDV → VPVRSCQGAEPHVRGPRQPR DQRPIHARRLAPRLAGRQGR QHLPVASAGRWGRGAGARHA LSNPLPVPRLLPRRLIAAWR DRLARRRGPAPPCPSLAQSP TTRGRLFPGLTSRHSRSRIF LEGANGAPAHTV in isoform 2. 1 PublicationVSP_024483Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK093146 mRNA. Translation: BAC04073.1. Different initiation.
AK128679 mRNA. Translation: BAC87566.1. Sequence problems.
BC108280 mRNA. Translation: AAI08281.1.
CCDSiCCDS76415.1. [Q32P44-2]
CCDS8023.2. [Q32P44-1]
RefSeqiNP_001287722.1. NM_001300793.1.
NP_694997.2. NM_153265.2. [Q32P44-1]
UniGeneiHs.379785.

Genome annotation databases

EnsembliENST00000394773; ENSP00000378254; ENSG00000149499.
ENST00000494448; ENSP00000431752; ENSG00000149499. [Q32P44-3]
ENST00000529309; ENSP00000434513; ENSG00000149499. [Q32P44-2]
GeneIDi256364.
KEGGihsa:256364.
UCSCiuc001ntt.1. human. [Q32P44-1]
uc010rly.1. human. [Q32P44-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK093146 mRNA. Translation: BAC04073.1. Different initiation.
AK128679 mRNA. Translation: BAC87566.1. Sequence problems.
BC108280 mRNA. Translation: AAI08281.1.
CCDSiCCDS76415.1. [Q32P44-2]
CCDS8023.2. [Q32P44-1]
RefSeqiNP_001287722.1. NM_001300793.1.
NP_694997.2. NM_153265.2. [Q32P44-1]
UniGeneiHs.379785.

3D structure databases

ProteinModelPortaliQ32P44.
SMRiQ32P44. Positions 216-870.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi129161. 6 interactions.
IntActiQ32P44. 3 interactions.
MINTiMINT-1682086.
STRINGi9606.ENSP00000378254.

PTM databases

PhosphoSiteiQ32P44.

Polymorphism and mutation databases

BioMutaiEML3.
DMDMi121945710.

Proteomic databases

MaxQBiQ32P44.
PaxDbiQ32P44.
PeptideAtlasiQ32P44.
PRIDEiQ32P44.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000394773; ENSP00000378254; ENSG00000149499.
ENST00000494448; ENSP00000431752; ENSG00000149499. [Q32P44-3]
ENST00000529309; ENSP00000434513; ENSG00000149499. [Q32P44-2]
GeneIDi256364.
KEGGihsa:256364.
UCSCiuc001ntt.1. human. [Q32P44-1]
uc010rly.1. human. [Q32P44-2]

Organism-specific databases

CTDi256364.
GeneCardsiGC11M062370.
HGNCiHGNC:26666. EML3.
neXtProtiNX_Q32P44.
PharmGKBiPA142671910.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00550000074369.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ32P44.
KOiK18596.
OMAiXETGLCA.
OrthoDBiEOG7XWPMW.
PhylomeDBiQ32P44.
TreeFamiTF317832.

Enzyme and pathway databases

SignaLinkiQ32P44.

Miscellaneous databases

ChiTaRSiEML3. human.
GenomeRNAii256364.
NextBioi92788.
PROiQ32P44.

Gene expression databases

BgeeiQ32P44.
CleanExiHS_EML3.
ExpressionAtlasiQ32P44. baseline and differential.
GenevisibleiQ32P44. HS.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR005108. HELP.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 3 hits.
PROSITEiPS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-896 (ISOFORM 2).
    Tissue: Testis and Uterus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Uterus.
  3. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
    Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
    Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176 AND THR-881, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  4. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
    Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
    J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-883, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiEMAL3_HUMAN
AccessioniPrimary (citable) accession number: Q32P44
Secondary accession number(s): Q6ZQW7, Q8NA55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: December 6, 2005
Last modified: July 22, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.