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Reviewed, UniProtKB/Swiss-Prot Q32P44 (EMAL3_HUMAN)

Last modified November 24, 2009. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Echinoderm microtubule-associated protein-like 3
      Short name=EMAP-3
Gene names
Name: EML3
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length896 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic By similarity.

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the WD repeat EMAP family.

Contains 9 WD repeats.

Ontologies

Keywords
   Cellular componentCytoplasm
Microtubule
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
WD repeat
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

microtubule

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionprotein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q32P44-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q32P44-2)

The sequence of this isoform differs from the canonical sequence as follows:
     786-896: GVWPDGSDGT...SLSPASSLDV → VPVRSCQGAE...GANGAPAHTV
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Natural variant7881V → M: dbSNP rs34709729.
Isoform 3 (identifier: Q32P44-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-163: MDGAAGPGDG...RNSSSSSSPS → MDPQPLRGPA...PCSPHSVLTQ
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 896896Echinoderm microtubule-associated protein-like 3
PRO_0000284390

Regions

Repeat303 – 35250WD 1
Repeat357 – 40044WD 2
Repeat403 – 44240WD 3
Repeat457 – 49539WD 4
Repeat512 – 55140WD 5
Repeat595 – 63440WD 6
Repeat678 – 71740WD 7
Repeat724 – 76340WD 8
Repeat837 – 87741WD 9
Compositional bias126 – 1294Poly-Ser
Compositional bias156 – 1638Poly-Ser
Compositional bias287 – 29711Poly-Gly

Amino acid modifications

Modified residue1761Phosphoserine Ref.3 Ref.5 Ref.7
Modified residue8811Phosphothreonine Ref.3 Ref.5 Ref.4

Natural variations

Alternative sequence1 – 163163MDGAA…SSSPS → MDPQPLRGPAKSLVGPNLKE GAAAAVVLAPLAPRGSSGPC SPHSVLTQ in isoform 3.
VSP_024482
Alternative sequence786 – 896111GVWPD…SSLDV → VPVRSCQGAEPHVRGPRQPR DQRPIHARRLAPRLAGRQGR QHLPVASAGRWGRGAGARHA LSNPLPVPRLLPRRLIAAWR DRLARRRGPAPPCPSLAQSP TTRGRLFPGLTSRHSRSRIF LEGANGAPAHTV in isoform 2.
VSP_024483
Natural variant6201Q → K: dbSNP rs34098002.
VAR_031725

Experimental info

Sequence conflict651S → SS in BAC04073. Ref.1
Sequence conflict3811D → N in BAC04073. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 21D2EBD29B969D34

FASTA89695,197
        10         20         30         40         50         60 
MDGAAGPGDG PAREALQSLS QRLRVQEQEM ELVKAALAEA LRLLRLQVPP SSLQGSGTPA 

        70         80         90        100        110        120 
PPGDSLAAPP GLPPTCTPSL VSRGTQTETE VELKSSPGPP GLSNGPPAPQ GASEEPSGTQ 

       130        140        150        160        170        180 
SEGGGSSSSG AGSPGPPGIL RPLQPPQRAD TPRRNSSSSS SPSERPRQKL SRKAISSANL 

       190        200        210        220        230        240 
LVRSGSTESR GGKDPLSSPG GPGSRRSNYN LEGISVKMFL RGRPITMYIP SGIRSLEELP 

       250        260        270        280        290        300 
SGPPPETLSL DWVYGYRGRD SRSNLFVLRS GEVVYFIACV VVLYRPGGGP GGPGGGGQRH 

       310        320        330        340        350        360 
YRGHTDCVRC LAVHPDGVRV ASGQTAGVDK DGKPLQPVVH IWDSETLLKL QEIGLGAFER 

       370        380        390        400        410        420 
GVGALAFSAA DQGAFLCVVD DSNEHMLSVW DCSRGMKLAE IKSTNDSVLA VGFNPRDSSC 

       430        440        450        460        470        480 
IVTSGKSHVH FWNWSGGVGV PGNGTLTRKQ GVFGKYKKPK FIPCFVFLPD GDILTGDSEG 

       490        500        510        520        530        540 
NILTWGRSPS DSKTPGRGGA KETYGIVAQA HAHEGSIFAL CLRRDGTVLS GGGRDRRLVQ 

       550        560        570        580        590        600 
WGPGLVALQE AEIPEHFGAV RAIAEGLGSE LLVGTTKNAL LRGDLAQGFS PVIQGHTDEL 

       610        620        630        640        650        660 
WGLCTHPSQN RFLTCGHDRQ LCLWDGESHA LAWSIDLKET GLCADFHPSG AVVAVGLNTG 

       670        680        690        700        710        720 
RWLVLDTETR EIVSDVIDGN EQLSVVRYSP DGLYLAIGSH DNVIYIYSVS SDGAKSSRFG 

       730        740        750        760        770        780 
RCMGHSSFIT HLDWSKDGNF IMSNSGDYEI LYWDVAGGCK QLKNRYESRD REWATYTCVL 

       790        800        810        820        830        840 
GFHVYGVWPD GSDGTDINSL CRSHNERVVA VADDFCKVHL FQYPCARAKA PSRMYGGHGS 

       850        860        870        880        890 
HVTSVRFTHD DSHLVSLGGK DASIFQWRVL GAGGAGPAPA TPSRTPSLSP ASSLDV 

« Hide

Isoform 2.

Checksum: C5E4325DCE7C226F
Show »

FASTA91797,987
Isoform 3.

Checksum: 38E55507F72F5D15
Show »

FASTA78183,729

References

[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-896 (ISOFORM 2).
Tissue: Testis and Uterus.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Uterus.
[3]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176 AND THR-881, MASS SPECTROMETRY.
Tissue: Epithelium.
[4]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-881, MASS SPECTROMETRY.
[5]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176 AND THR-881, MASS SPECTROMETRY.
[6]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[7]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, MASS SPECTROMETRY.
Tissue: T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK093146 mRNA. Translation: BAC04073.1. Different initiation.
AK128679 mRNA. Translation: BAC87566.1.
BC108280 mRNA. Translation: AAI08281.1.
IPIIPI00167909.
IPI00843902.
IPI00843958.
RefSeqNP_694997.2.
UniGeneHs.379785

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActQ32P44. 3 interactions.
STRINGQ32P44.

PTM databases

PhosphoSiteQ32P44.

Proteomic databases

PeptideAtlasQ32P44.
PRIDEQ32P44.

Genome annotation databases

EnsemblENST00000394773; ENSP00000378254; ENSG00000149499; Homo sapiens. [Genome view]
GeneID256364.
KEGGhsa:256364.
UCSCuc001ntr.1. human.
uc001nts.1. human.
uc001ntt.1. human.

Organism-specific databases

CTD256364.
GeneCardsGC11M062127.
HGNCHGNC:26666. EML3.
PharmGKBPA142671910.
GenAtlasSearch...

Phylogenomic databases

HOVERGENQ32P44.
OrthoDBEOG9KD95C.

Gene expression databases

ArrayExpressQ32P44.
BgeeQ32P44.
CleanExHS_EML3.
GenevestigatorQ32P44.

Family and domain databases

InterProIPR005108. HELP.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR011046. WD40_repeat-like_dom.
IPR019782. WD40_repeat_2.
IPR017986. WD40_repeat_dom.
IPR019781. WD40_repeat_sg.
[Graphical view]
Gene3DG3DSA:2.130.10.10. WD40/YVTN_repeat-like. 2 hits.
PfamPF03451. HELP. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTSM00320. WD40. 8 hits.
[Graphical view]
PROSITEPS00678. WD_REPEATS_1. False negative.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio92788.

Entry information

Entry nameEMAL3_HUMAN
AccessionPrimary (citable) accession number: Q32P44
Secondary accession number(s): Q6ZQW7, Q8NA55
Entry history
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: December 6, 2005
Last modified: November 24, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Human chromosome 11: entries, gene names and cross-references to MIM

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List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents