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Q32P44 (EMAL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Echinoderm microtubule-associated protein-like 3

Short name=EMAP-3
Gene names
Name:EML3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length896 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic By similarity.

Subcellular location

Cytoplasmcytoskeleton Potential.

Sequence similarities

Belongs to the WD repeat EMAP family.

Contains 9 WD repeats.

Sequence caution

The sequence BAC04073.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC87566.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
Microtubule
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
WD repeat
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q32P44-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q32P44-2)

The sequence of this isoform differs from the canonical sequence as follows:
     786-896: GVWPDGSDGT...SLSPASSLDV → VPVRSCQGAE...GANGAPAHTV
Note: No experimental confirmation available.
Isoform 3 (identifier: Q32P44-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.
     152-223: PRRNSSSSSS...ISVKMFLRGR → AASAEALQEG...HVHPVWHPQP
     224-896: Missing.
Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 896896Echinoderm microtubule-associated protein-like 3
PRO_0000284390

Regions

Repeat303 – 35250WD 1
Repeat357 – 40044WD 2
Repeat403 – 44240WD 3
Repeat457 – 49539WD 4
Repeat512 – 55140WD 5
Repeat595 – 63440WD 6
Repeat678 – 71740WD 7
Repeat724 – 76340WD 8
Repeat837 – 87741WD 9
Compositional bias126 – 1294Poly-Ser
Compositional bias156 – 1638Poly-Ser
Compositional bias287 – 29711Poly-Gly

Amino acid modifications

Modified residue11N-acetylmethionine Ref.10
Modified residue1761Phosphoserine Ref.3 Ref.5 Ref.6 Ref.7 Ref.9
Modified residue8811Phosphothreonine Ref.3

Natural variations

Alternative sequence1 – 2929Missing in isoform 3.
VSP_039926
Alternative sequence152 – 22372PRRNS…FLRGR → AASAEALQEGNLLRQPVSAV REHREPWGKRPPLQPWGPWI SEEQLQFGRHLSEDVPSRAP HYHVHPVWHPQP in isoform 3.
VSP_039927
Alternative sequence224 – 896673Missing in isoform 3.
VSP_039928
Alternative sequence786 – 896111GVWPD…SSLDV → VPVRSCQGAEPHVRGPRQPR DQRPIHARRLAPRLAGRQGR QHLPVASAGRWGRGAGARHA LSNPLPVPRLLPRRLIAAWR DRLARRRGPAPPCPSLAQSP TTRGRLFPGLTSRHSRSRIF LEGANGAPAHTV in isoform 2.
VSP_024483
Natural variant6201Q → K.
Corresponds to variant rs34098002 [ dbSNP | Ensembl ].
VAR_031725
Isoform 2:
Natural variant7881V → M.
Corresponds to variant rs34709729 [ dbSNP | Ensembl ].

Experimental info

Sequence conflict651S → SS in BAC04073. Ref.1
Sequence conflict3811D → N in BAC04073. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 21D2EBD29B969D34

FASTA89695,197
        10         20         30         40         50         60 
MDGAAGPGDG PAREALQSLS QRLRVQEQEM ELVKAALAEA LRLLRLQVPP SSLQGSGTPA 

        70         80         90        100        110        120 
PPGDSLAAPP GLPPTCTPSL VSRGTQTETE VELKSSPGPP GLSNGPPAPQ GASEEPSGTQ 

       130        140        150        160        170        180 
SEGGGSSSSG AGSPGPPGIL RPLQPPQRAD TPRRNSSSSS SPSERPRQKL SRKAISSANL 

       190        200        210        220        230        240 
LVRSGSTESR GGKDPLSSPG GPGSRRSNYN LEGISVKMFL RGRPITMYIP SGIRSLEELP 

       250        260        270        280        290        300 
SGPPPETLSL DWVYGYRGRD SRSNLFVLRS GEVVYFIACV VVLYRPGGGP GGPGGGGQRH 

       310        320        330        340        350        360 
YRGHTDCVRC LAVHPDGVRV ASGQTAGVDK DGKPLQPVVH IWDSETLLKL QEIGLGAFER 

       370        380        390        400        410        420 
GVGALAFSAA DQGAFLCVVD DSNEHMLSVW DCSRGMKLAE IKSTNDSVLA VGFNPRDSSC 

       430        440        450        460        470        480 
IVTSGKSHVH FWNWSGGVGV PGNGTLTRKQ GVFGKYKKPK FIPCFVFLPD GDILTGDSEG 

       490        500        510        520        530        540 
NILTWGRSPS DSKTPGRGGA KETYGIVAQA HAHEGSIFAL CLRRDGTVLS GGGRDRRLVQ 

       550        560        570        580        590        600 
WGPGLVALQE AEIPEHFGAV RAIAEGLGSE LLVGTTKNAL LRGDLAQGFS PVIQGHTDEL 

       610        620        630        640        650        660 
WGLCTHPSQN RFLTCGHDRQ LCLWDGESHA LAWSIDLKET GLCADFHPSG AVVAVGLNTG 

       670        680        690        700        710        720 
RWLVLDTETR EIVSDVIDGN EQLSVVRYSP DGLYLAIGSH DNVIYIYSVS SDGAKSSRFG 

       730        740        750        760        770        780 
RCMGHSSFIT HLDWSKDGNF IMSNSGDYEI LYWDVAGGCK QLKNRYESRD REWATYTCVL 

       790        800        810        820        830        840 
GFHVYGVWPD GSDGTDINSL CRSHNERVVA VADDFCKVHL FQYPCARAKA PSRMYGGHGS 

       850        860        870        880        890 
HVTSVRFTHD DSHLVSLGGK DASIFQWRVL GAGGAGPAPA TPSRTPSLSP ASSLDV 

« Hide

Isoform 2 [UniParc].

Checksum: C5E4325DCE7C226F
Show »

FASTA91797,987
Isoform 3 [UniParc].

Checksum: FBA484EF88EBA319
Show »

FASTA19420,224

References

[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-896 (ISOFORM 2).
Tissue: Testis and Uterus.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Uterus.
[3]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176 AND THR-881, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[4]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[5]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[7]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK093146 mRNA. Translation: BAC04073.1. Different initiation.
AK128679 mRNA. Translation: BAC87566.1. Sequence problems.
BC108280 mRNA. Translation: AAI08281.1.
RefSeqNP_694997.2. NM_153265.2.
XP_005273934.1. XM_005273877.2.
UniGeneHs.379785.

3D structure databases

ProteinModelPortalQ32P44.
SMRQ32P44. Positions 501-869.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid129161. 6 interactions.
IntActQ32P44. 3 interactions.
MINTMINT-1682086.
STRING9606.ENSP00000378254.

PTM databases

PhosphoSiteQ32P44.

Polymorphism databases

DMDM121945710.

Proteomic databases

PaxDbQ32P44.
PeptideAtlasQ32P44.
PRIDEQ32P44.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000394773; ENSP00000378254; ENSG00000149499. [Q32P44-1]
ENST00000494448; ENSP00000431752; ENSG00000149499. [Q32P44-3]
ENST00000529309; ENSP00000434513; ENSG00000149499. [Q32P44-2]
GeneID256364.
KEGGhsa:256364.
UCSCuc001ntt.1. human. [Q32P44-1]
uc010rly.1. human. [Q32P44-2]

Organism-specific databases

CTD256364.
GeneCardsGC11M062370.
HGNCHGNC:26666. EML3.
neXtProtNX_Q32P44.
PharmGKBPA142671910.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2319.
HOGENOMHOG000294061.
HOVERGENHBG051470.
OMAKAPSRMY.
OrthoDBEOG7XWPMW.
PhylomeDBQ32P44.
TreeFamTF317832.

Enzyme and pathway databases

SignaLinkQ32P44.

Gene expression databases

ArrayExpressQ32P44.
BgeeQ32P44.
CleanExHS_EML3.
GenevestigatorQ32P44.

Family and domain databases

Gene3D2.130.10.10. 3 hits.
InterProIPR005108. HELP.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF03451. HELP. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTSM00320. WD40. 8 hits.
[Graphical view]
SUPFAMSSF50998. SSF50998. 3 hits.
PROSITEPS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSEML3. human.
GenomeRNAi256364.
NextBio92788.
PROQ32P44.

Entry information

Entry nameEMAL3_HUMAN
AccessionPrimary (citable) accession number: Q32P44
Secondary accession number(s): Q6ZQW7, Q8NA55
Entry history
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: December 6, 2005
Last modified: April 16, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM