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Protein

Echinoderm microtubule-associated protein-like 3

Gene

EML3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

SignaLinkiQ32P44.

Names & Taxonomyi

Protein namesi
Recommended name:
Echinoderm microtubule-associated protein-like 3
Short name:
EMAP-3
Gene namesi
Name:EML3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:26666. EML3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000149499.
PharmGKBiPA142671910.

Polymorphism and mutation databases

BioMutaiEML3.
DMDMi121945710.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002843901 – 896Echinoderm microtubule-associated protein-like 3Add BLAST896

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei176PhosphoserineCombined sources1
Modified residuei198PhosphoserineCombined sources1
Modified residuei204PhosphoserineCombined sources1
Modified residuei881PhosphothreonineCombined sources1
Modified residuei883PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ32P44.
MaxQBiQ32P44.
PaxDbiQ32P44.
PeptideAtlasiQ32P44.
PRIDEiQ32P44.

PTM databases

iPTMnetiQ32P44.
PhosphoSitePlusiQ32P44.

Expressioni

Gene expression databases

BgeeiENSG00000149499.
CleanExiHS_EML3.
ExpressionAtlasiQ32P44. baseline and differential.
GenevisibleiQ32P44. HS.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi129161. 13 interactors.
IntActiQ32P44. 7 interactors.
MINTiMINT-1682086.
STRINGi9606.ENSP00000378254.

Structurei

3D structure databases

ProteinModelPortaliQ32P44.
SMRiQ32P44.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati303 – 352WD 1Add BLAST50
Repeati357 – 400WD 2Add BLAST44
Repeati403 – 442WD 3Add BLAST40
Repeati457 – 495WD 4Add BLAST39
Repeati512 – 551WD 5Add BLAST40
Repeati595 – 634WD 6Add BLAST40
Repeati678 – 717WD 7Add BLAST40
Repeati724 – 763WD 8Add BLAST40
Repeati837 – 877WD 9Add BLAST41

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi126 – 129Poly-Ser4
Compositional biasi156 – 163Poly-Ser8
Compositional biasi287 – 297Poly-GlyAdd BLAST11

Sequence similaritiesi

Belongs to the WD repeat EMAP family.Curated
Contains 9 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2106. Eukaryota.
ENOG410XNTQ. LUCA.
GeneTreeiENSGT00550000074369.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ32P44.
KOiK18596.
OMAiXETGLCA.
OrthoDBiEOG091G04Z4.
PhylomeDBiQ32P44.
TreeFamiTF317832.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR005108. HELP.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
SMARTiSM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 3 hits.
PROSITEiPS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q32P44-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGAAGPGDG PAREALQSLS QRLRVQEQEM ELVKAALAEA LRLLRLQVPP
60 70 80 90 100
SSLQGSGTPA PPGDSLAAPP GLPPTCTPSL VSRGTQTETE VELKSSPGPP
110 120 130 140 150
GLSNGPPAPQ GASEEPSGTQ SEGGGSSSSG AGSPGPPGIL RPLQPPQRAD
160 170 180 190 200
TPRRNSSSSS SPSERPRQKL SRKAISSANL LVRSGSTESR GGKDPLSSPG
210 220 230 240 250
GPGSRRSNYN LEGISVKMFL RGRPITMYIP SGIRSLEELP SGPPPETLSL
260 270 280 290 300
DWVYGYRGRD SRSNLFVLRS GEVVYFIACV VVLYRPGGGP GGPGGGGQRH
310 320 330 340 350
YRGHTDCVRC LAVHPDGVRV ASGQTAGVDK DGKPLQPVVH IWDSETLLKL
360 370 380 390 400
QEIGLGAFER GVGALAFSAA DQGAFLCVVD DSNEHMLSVW DCSRGMKLAE
410 420 430 440 450
IKSTNDSVLA VGFNPRDSSC IVTSGKSHVH FWNWSGGVGV PGNGTLTRKQ
460 470 480 490 500
GVFGKYKKPK FIPCFVFLPD GDILTGDSEG NILTWGRSPS DSKTPGRGGA
510 520 530 540 550
KETYGIVAQA HAHEGSIFAL CLRRDGTVLS GGGRDRRLVQ WGPGLVALQE
560 570 580 590 600
AEIPEHFGAV RAIAEGLGSE LLVGTTKNAL LRGDLAQGFS PVIQGHTDEL
610 620 630 640 650
WGLCTHPSQN RFLTCGHDRQ LCLWDGESHA LAWSIDLKET GLCADFHPSG
660 670 680 690 700
AVVAVGLNTG RWLVLDTETR EIVSDVIDGN EQLSVVRYSP DGLYLAIGSH
710 720 730 740 750
DNVIYIYSVS SDGAKSSRFG RCMGHSSFIT HLDWSKDGNF IMSNSGDYEI
760 770 780 790 800
LYWDVAGGCK QLKNRYESRD REWATYTCVL GFHVYGVWPD GSDGTDINSL
810 820 830 840 850
CRSHNERVVA VADDFCKVHL FQYPCARAKA PSRMYGGHGS HVTSVRFTHD
860 870 880 890
DSHLVSLGGK DASIFQWRVL GAGGAGPAPA TPSRTPSLSP ASSLDV
Length:896
Mass (Da):95,197
Last modified:December 6, 2005 - v1
Checksum:i21D2EBD29B969D34
GO
Isoform 2 (identifier: Q32P44-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     786-896: GVWPDGSDGT...SLSPASSLDV → VPVRSCQGAE...GANGAPAHTV

Note: No experimental confirmation available.
Show »
Length:917
Mass (Da):97,987
Checksum:iC5E4325DCE7C226F
GO
Isoform 3 (identifier: Q32P44-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.
     152-223: PRRNSSSSSS...ISVKMFLRGR → AASAEALQEG...HVHPVWHPQP
     224-896: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:194
Mass (Da):20,224
Checksum:iFBA484EF88EBA319
GO

Sequence cautioni

The sequence BAC04073 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC87566 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65S → SS in BAC04073 (PubMed:14702039).Curated1
Sequence conflicti381D → N in BAC04073 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031725620Q → K.Corresponds to variant rs34098002dbSNPEnsembl.1
Isoform 2 (identifier: Q32P44-2)
Natural varianti788V → M.Corresponds to variant rs34709729dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0399261 – 29Missing in isoform 3. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_039927152 – 223PRRNS…FLRGR → AASAEALQEGNLLRQPVSAV REHREPWGKRPPLQPWGPWI SEEQLQFGRHLSEDVPSRAP HYHVHPVWHPQP in isoform 3. 1 PublicationAdd BLAST72
Alternative sequenceiVSP_039928224 – 896Missing in isoform 3. 1 PublicationAdd BLAST673
Alternative sequenceiVSP_024483786 – 896GVWPD…SSLDV → VPVRSCQGAEPHVRGPRQPR DQRPIHARRLAPRLAGRQGR QHLPVASAGRWGRGAGARHA LSNPLPVPRLLPRRLIAAWR DRLARRRGPAPPCPSLAQSP TTRGRLFPGLTSRHSRSRIF LEGANGAPAHTV in isoform 2. 1 PublicationAdd BLAST111

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK093146 mRNA. Translation: BAC04073.1. Different initiation.
AK128679 mRNA. Translation: BAC87566.1. Sequence problems.
BC108280 mRNA. Translation: AAI08281.1.
CCDSiCCDS76415.1. [Q32P44-2]
CCDS8023.2. [Q32P44-1]
RefSeqiNP_001287722.1. NM_001300793.1.
NP_694997.2. NM_153265.2. [Q32P44-1]
UniGeneiHs.379785.

Genome annotation databases

EnsembliENST00000394773; ENSP00000378254; ENSG00000149499. [Q32P44-1]
ENST00000494448; ENSP00000431752; ENSG00000149499. [Q32P44-3]
ENST00000529309; ENSP00000434513; ENSG00000149499. [Q32P44-2]
GeneIDi256364.
KEGGihsa:256364.
UCSCiuc001ntu.2. human. [Q32P44-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK093146 mRNA. Translation: BAC04073.1. Different initiation.
AK128679 mRNA. Translation: BAC87566.1. Sequence problems.
BC108280 mRNA. Translation: AAI08281.1.
CCDSiCCDS76415.1. [Q32P44-2]
CCDS8023.2. [Q32P44-1]
RefSeqiNP_001287722.1. NM_001300793.1.
NP_694997.2. NM_153265.2. [Q32P44-1]
UniGeneiHs.379785.

3D structure databases

ProteinModelPortaliQ32P44.
SMRiQ32P44.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi129161. 13 interactors.
IntActiQ32P44. 7 interactors.
MINTiMINT-1682086.
STRINGi9606.ENSP00000378254.

PTM databases

iPTMnetiQ32P44.
PhosphoSitePlusiQ32P44.

Polymorphism and mutation databases

BioMutaiEML3.
DMDMi121945710.

Proteomic databases

EPDiQ32P44.
MaxQBiQ32P44.
PaxDbiQ32P44.
PeptideAtlasiQ32P44.
PRIDEiQ32P44.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000394773; ENSP00000378254; ENSG00000149499. [Q32P44-1]
ENST00000494448; ENSP00000431752; ENSG00000149499. [Q32P44-3]
ENST00000529309; ENSP00000434513; ENSG00000149499. [Q32P44-2]
GeneIDi256364.
KEGGihsa:256364.
UCSCiuc001ntu.2. human. [Q32P44-1]

Organism-specific databases

CTDi256364.
GeneCardsiEML3.
HGNCiHGNC:26666. EML3.
neXtProtiNX_Q32P44.
OpenTargetsiENSG00000149499.
PharmGKBiPA142671910.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2106. Eukaryota.
ENOG410XNTQ. LUCA.
GeneTreeiENSGT00550000074369.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ32P44.
KOiK18596.
OMAiXETGLCA.
OrthoDBiEOG091G04Z4.
PhylomeDBiQ32P44.
TreeFamiTF317832.

Enzyme and pathway databases

SignaLinkiQ32P44.

Miscellaneous databases

ChiTaRSiEML3. human.
GenomeRNAii256364.
PROiQ32P44.

Gene expression databases

BgeeiENSG00000149499.
CleanExiHS_EML3.
ExpressionAtlasiQ32P44. baseline and differential.
GenevisibleiQ32P44. HS.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR005108. HELP.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
SMARTiSM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 3 hits.
PROSITEiPS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEMAL3_HUMAN
AccessioniPrimary (citable) accession number: Q32P44
Secondary accession number(s): Q6ZQW7, Q8NA55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: December 6, 2005
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.