SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q32P44

- EMAL3_HUMAN

UniProt

Q32P44 - EMAL3_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Echinoderm microtubule-associated protein-like 3
Gene
EML3
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic By similarity.

Enzyme and pathway databases

SignaLinkiQ32P44.

Names & Taxonomyi

Protein namesi
Recommended name:
Echinoderm microtubule-associated protein-like 3
Short name:
EMAP-3
Gene namesi
Name:EML3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:26666. EML3.

Subcellular locationi

Cytoplasmcytoskeleton Reviewed prediction

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. microtubule Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671910.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 896896Echinoderm microtubule-associated protein-like 3
PRO_0000284390Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei176 – 1761Phosphoserine5 Publications
Modified residuei881 – 8811Phosphothreonine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ32P44.
PaxDbiQ32P44.
PeptideAtlasiQ32P44.
PRIDEiQ32P44.

PTM databases

PhosphoSiteiQ32P44.

Expressioni

Gene expression databases

ArrayExpressiQ32P44.
BgeeiQ32P44.
CleanExiHS_EML3.
GenevestigatoriQ32P44.

Interactioni

Protein-protein interaction databases

BioGridi129161. 6 interactions.
IntActiQ32P44. 3 interactions.
MINTiMINT-1682086.
STRINGi9606.ENSP00000378254.

Structurei

3D structure databases

ProteinModelPortaliQ32P44.
SMRiQ32P44. Positions 361-392, 501-869.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati303 – 35250WD 1
Add
BLAST
Repeati357 – 40044WD 2
Add
BLAST
Repeati403 – 44240WD 3
Add
BLAST
Repeati457 – 49539WD 4
Add
BLAST
Repeati512 – 55140WD 5
Add
BLAST
Repeati595 – 63440WD 6
Add
BLAST
Repeati678 – 71740WD 7
Add
BLAST
Repeati724 – 76340WD 8
Add
BLAST
Repeati837 – 87741WD 9
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi126 – 1294Poly-Ser
Compositional biasi156 – 1638Poly-Ser
Compositional biasi287 – 29711Poly-Gly
Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat EMAP family.
Contains 9 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
OMAiKAPSRMY.
OrthoDBiEOG7XWPMW.
PhylomeDBiQ32P44.
TreeFamiTF317832.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR005108. HELP.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 3 hits.
PROSITEiPS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q32P44-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDGAAGPGDG PAREALQSLS QRLRVQEQEM ELVKAALAEA LRLLRLQVPP    50
SSLQGSGTPA PPGDSLAAPP GLPPTCTPSL VSRGTQTETE VELKSSPGPP 100
GLSNGPPAPQ GASEEPSGTQ SEGGGSSSSG AGSPGPPGIL RPLQPPQRAD 150
TPRRNSSSSS SPSERPRQKL SRKAISSANL LVRSGSTESR GGKDPLSSPG 200
GPGSRRSNYN LEGISVKMFL RGRPITMYIP SGIRSLEELP SGPPPETLSL 250
DWVYGYRGRD SRSNLFVLRS GEVVYFIACV VVLYRPGGGP GGPGGGGQRH 300
YRGHTDCVRC LAVHPDGVRV ASGQTAGVDK DGKPLQPVVH IWDSETLLKL 350
QEIGLGAFER GVGALAFSAA DQGAFLCVVD DSNEHMLSVW DCSRGMKLAE 400
IKSTNDSVLA VGFNPRDSSC IVTSGKSHVH FWNWSGGVGV PGNGTLTRKQ 450
GVFGKYKKPK FIPCFVFLPD GDILTGDSEG NILTWGRSPS DSKTPGRGGA 500
KETYGIVAQA HAHEGSIFAL CLRRDGTVLS GGGRDRRLVQ WGPGLVALQE 550
AEIPEHFGAV RAIAEGLGSE LLVGTTKNAL LRGDLAQGFS PVIQGHTDEL 600
WGLCTHPSQN RFLTCGHDRQ LCLWDGESHA LAWSIDLKET GLCADFHPSG 650
AVVAVGLNTG RWLVLDTETR EIVSDVIDGN EQLSVVRYSP DGLYLAIGSH 700
DNVIYIYSVS SDGAKSSRFG RCMGHSSFIT HLDWSKDGNF IMSNSGDYEI 750
LYWDVAGGCK QLKNRYESRD REWATYTCVL GFHVYGVWPD GSDGTDINSL 800
CRSHNERVVA VADDFCKVHL FQYPCARAKA PSRMYGGHGS HVTSVRFTHD 850
DSHLVSLGGK DASIFQWRVL GAGGAGPAPA TPSRTPSLSP ASSLDV 896
Length:896
Mass (Da):95,197
Last modified:December 6, 2005 - v1
Checksum:i21D2EBD29B969D34
GO
Isoform 2 (identifier: Q32P44-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     786-896: GVWPDGSDGT...SLSPASSLDV → VPVRSCQGAE...GANGAPAHTV

Note: No experimental confirmation available.

Show »
Length:917
Mass (Da):97,987
Checksum:iC5E4325DCE7C226F
GO
Isoform 3 (identifier: Q32P44-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.
     152-223: PRRNSSSSSS...ISVKMFLRGR → AASAEALQEG...HVHPVWHPQP
     224-896: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Show »
Length:194
Mass (Da):20,224
Checksum:iFBA484EF88EBA319
GO

Sequence cautioni

The sequence BAC04073.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti620 – 6201Q → K.
Corresponds to variant rs34098002 [ dbSNP | Ensembl ].
VAR_031725
Isoform 2 (identifier: Q32P44-2)
Natural varianti788 – 7881V → M.
Corresponds to variant rs34709729 [ dbSNP | Ensembl ].

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2929Missing in isoform 3.
VSP_039926Add
BLAST
Alternative sequencei152 – 22372PRRNS…FLRGR → AASAEALQEGNLLRQPVSAV REHREPWGKRPPLQPWGPWI SEEQLQFGRHLSEDVPSRAP HYHVHPVWHPQP in isoform 3.
VSP_039927Add
BLAST
Alternative sequencei224 – 896673Missing in isoform 3.
VSP_039928Add
BLAST
Alternative sequencei786 – 896111GVWPD…SSLDV → VPVRSCQGAEPHVRGPRQPR DQRPIHARRLAPRLAGRQGR QHLPVASAGRWGRGAGARHA LSNPLPVPRLLPRRLIAAWR DRLARRRGPAPPCPSLAQSP TTRGRLFPGLTSRHSRSRIF LEGANGAPAHTV in isoform 2.
VSP_024483Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651S → SS in BAC04073. 1 Publication
Sequence conflicti381 – 3811D → N in BAC04073. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK093146 mRNA. Translation: BAC04073.1. Different initiation.
AK128679 mRNA. Translation: BAC87566.1. Sequence problems.
BC108280 mRNA. Translation: AAI08281.1.
CCDSiCCDS8023.2. [Q32P44-1]
RefSeqiNP_694997.2. NM_153265.2. [Q32P44-1]
XP_005273934.1. XM_005273877.2. [Q32P44-2]
UniGeneiHs.379785.

Genome annotation databases

EnsembliENST00000394773; ENSP00000378254; ENSG00000149499. [Q32P44-1]
ENST00000494448; ENSP00000431752; ENSG00000149499. [Q32P44-3]
ENST00000529309; ENSP00000434513; ENSG00000149499. [Q32P44-2]
GeneIDi256364.
KEGGihsa:256364.
UCSCiuc001ntt.1. human. [Q32P44-1]
uc010rly.1. human. [Q32P44-2]

Polymorphism databases

DMDMi121945710.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK093146 mRNA. Translation: BAC04073.1 . Different initiation.
AK128679 mRNA. Translation: BAC87566.1 . Sequence problems.
BC108280 mRNA. Translation: AAI08281.1 .
CCDSi CCDS8023.2. [Q32P44-1 ]
RefSeqi NP_694997.2. NM_153265.2. [Q32P44-1 ]
XP_005273934.1. XM_005273877.2. [Q32P44-2 ]
UniGenei Hs.379785.

3D structure databases

ProteinModelPortali Q32P44.
SMRi Q32P44. Positions 361-392, 501-869.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 129161. 6 interactions.
IntActi Q32P44. 3 interactions.
MINTi MINT-1682086.
STRINGi 9606.ENSP00000378254.

PTM databases

PhosphoSitei Q32P44.

Polymorphism databases

DMDMi 121945710.

Proteomic databases

MaxQBi Q32P44.
PaxDbi Q32P44.
PeptideAtlasi Q32P44.
PRIDEi Q32P44.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000394773 ; ENSP00000378254 ; ENSG00000149499 . [Q32P44-1 ]
ENST00000494448 ; ENSP00000431752 ; ENSG00000149499 . [Q32P44-3 ]
ENST00000529309 ; ENSP00000434513 ; ENSG00000149499 . [Q32P44-2 ]
GeneIDi 256364.
KEGGi hsa:256364.
UCSCi uc001ntt.1. human. [Q32P44-1 ]
uc010rly.1. human. [Q32P44-2 ]

Organism-specific databases

CTDi 256364.
GeneCardsi GC11M062370.
HGNCi HGNC:26666. EML3.
neXtProti NX_Q32P44.
PharmGKBi PA142671910.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG2319.
HOGENOMi HOG000294061.
HOVERGENi HBG051470.
OMAi KAPSRMY.
OrthoDBi EOG7XWPMW.
PhylomeDBi Q32P44.
TreeFami TF317832.

Enzyme and pathway databases

SignaLinki Q32P44.

Miscellaneous databases

ChiTaRSi EML3. human.
GenomeRNAii 256364.
NextBioi 92788.
PROi Q32P44.

Gene expression databases

ArrayExpressi Q32P44.
Bgeei Q32P44.
CleanExi HS_EML3.
Genevestigatori Q32P44.

Family and domain databases

Gene3Di 2.130.10.10. 3 hits.
InterProi IPR005108. HELP.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF03451. HELP. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view ]
SMARTi SM00320. WD40. 8 hits.
[Graphical view ]
SUPFAMi SSF50998. SSF50998. 3 hits.
PROSITEi PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-896 (ISOFORM 2).
    Tissue: Testis and Uterus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Uterus.
  3. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
    Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
    Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176 AND THR-881, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  4. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
    Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
    J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEMAL3_HUMAN
AccessioniPrimary (citable) accession number: Q32P44
Secondary accession number(s): Q6ZQW7, Q8NA55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: December 6, 2005
Last modified: July 9, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi