Q32P44 (EMAL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Echinoderm microtubule-associated protein-like 3
Short name=EMAP-3

Gene names

Name:

EML3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	896 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic By similarity.
Subcellular location	Cytoplasm › cytoskeleton Potential.
Sequence similarities	Belongs to the WD repeat EMAP family. Contains 9 WD repeats.
Sequence caution	The sequence BAC04073.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC87566.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q32P44-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q32P44-2)

The sequence of this isoform differs from the canonical sequence as follows:
     786-896: GVWPDGSDGT...SLSPASSLDV → VPVRSCQGAE...GANGAPAHTV

Note: No experimental confirmation available.

Isoform 3 (identifier: Q32P44-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.
     152-223: PRRNSSSSSS...ISVKMFLRGR → AASAEALQEG...HVHPVWHPQP
     224-896: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 896	896	Echinoderm microtubule-associated protein-like 3		PRO_0000284390
Regions
	Repeat	303 – 352	50	WD 1
	Repeat	357 – 400	44	WD 2
	Repeat	403 – 442	40	WD 3
	Repeat	457 – 495	39	WD 4
	Repeat	512 – 551	40	WD 5
	Repeat	595 – 634	40	WD 6
	Repeat	678 – 717	40	WD 7
	Repeat	724 – 763	40	WD 8
	Repeat	837 – 877	41	WD 9
	Compositional bias	126 – 129	4	Poly-Ser
	Compositional bias	156 – 163	8	Poly-Ser
	Compositional bias	287 – 297	11	Poly-Gly
Amino acid modifications
	Modified residue	176	1	Phosphoserine Ref.3 Ref.5 Ref.6 Ref.7 Ref.9
	Modified residue	881	1	Phosphothreonine Ref.3
Natural variations
	Alternative sequence	1 – 29	29	Missing in isoform 3.		VSP_039926
	Alternative sequence	152 – 223	72	PRRNS…FLRGR → AASAEALQEGNLLRQPVSAV REHREPWGKRPPLQPWGPWI SEEQLQFGRHLSEDVPSRAP HYHVHPVWHPQP in isoform 3.		VSP_039927
	Alternative sequence	224 – 896	673	Missing in isoform 3.		VSP_039928
	Alternative sequence	786 – 896	111	GVWPD…SSLDV → VPVRSCQGAEPHVRGPRQPR DQRPIHARRLAPRLAGRQGR QHLPVASAGRWGRGAGARHA LSNPLPVPRLLPRRLIAAWR DRLARRRGPAPPCPSLAQSP TTRGRLFPGLTSRHSRSRIF LEGANGAPAHTV in isoform 2.		VSP_024483
	Natural variant	620	1	Q → K. Corresponds to variant rs34098002 [ dbSNP | Ensembl ].		VAR_031725
Isoform 2:
	Natural variant	788	1	V → M. Corresponds to variant rs34709729 [ dbSNP | Ensembl ].
Experimental info
	Sequence conflict	65	1	S → SS in BAC04073. Ref.1
	Sequence conflict	381	1	D → N in BAC04073. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 21D2EBD29B969D34	FASTA	896	95,197
	10 20 30 40 50 60 MDGAAGPGDG PAREALQSLS QRLRVQEQEM ELVKAALAEA LRLLRLQVPP SSLQGSGTPA 70 80 90 100 110 120 PPGDSLAAPP GLPPTCTPSL VSRGTQTETE VELKSSPGPP GLSNGPPAPQ GASEEPSGTQ 130 140 150 160 170 180 SEGGGSSSSG AGSPGPPGIL RPLQPPQRAD TPRRNSSSSS SPSERPRQKL SRKAISSANL 190 200 210 220 230 240 LVRSGSTESR GGKDPLSSPG GPGSRRSNYN LEGISVKMFL RGRPITMYIP SGIRSLEELP 250 260 270 280 290 300 SGPPPETLSL DWVYGYRGRD SRSNLFVLRS GEVVYFIACV VVLYRPGGGP GGPGGGGQRH 310 320 330 340 350 360 YRGHTDCVRC LAVHPDGVRV ASGQTAGVDK DGKPLQPVVH IWDSETLLKL QEIGLGAFER 370 380 390 400 410 420 GVGALAFSAA DQGAFLCVVD DSNEHMLSVW DCSRGMKLAE IKSTNDSVLA VGFNPRDSSC 430 440 450 460 470 480 IVTSGKSHVH FWNWSGGVGV PGNGTLTRKQ GVFGKYKKPK FIPCFVFLPD GDILTGDSEG 490 500 510 520 530 540 NILTWGRSPS DSKTPGRGGA KETYGIVAQA HAHEGSIFAL CLRRDGTVLS GGGRDRRLVQ 550 560 570 580 590 600 WGPGLVALQE AEIPEHFGAV RAIAEGLGSE LLVGTTKNAL LRGDLAQGFS PVIQGHTDEL 610 620 630 640 650 660 WGLCTHPSQN RFLTCGHDRQ LCLWDGESHA LAWSIDLKET GLCADFHPSG AVVAVGLNTG 670 680 690 700 710 720 RWLVLDTETR EIVSDVIDGN EQLSVVRYSP DGLYLAIGSH DNVIYIYSVS SDGAKSSRFG 730 740 750 760 770 780 RCMGHSSFIT HLDWSKDGNF IMSNSGDYEI LYWDVAGGCK QLKNRYESRD REWATYTCVL 790 800 810 820 830 840 GFHVYGVWPD GSDGTDINSL CRSHNERVVA VADDFCKVHL FQYPCARAKA PSRMYGGHGS 850 860 870 880 890 HVTSVRFTHD DSHLVSLGGK DASIFQWRVL GAGGAGPAPA TPSRTPSLSP ASSLDV
	Isoform 2 [UniParc]. Checksum: C5E4325DCE7C226F	FASTA	917	97,987
	10 20 30 40 50 60 MDGAAGPGDG PAREALQSLS QRLRVQEQEM ELVKAALAEA LRLLRLQVPP SSLQGSGTPA 70 80 90 100 110 120 PPGDSLAAPP GLPPTCTPSL VSRGTQTETE VELKSSPGPP GLSNGPPAPQ GASEEPSGTQ 130 140 150 160 170 180 SEGGGSSSSG AGSPGPPGIL RPLQPPQRAD TPRRNSSSSS SPSERPRQKL SRKAISSANL 190 200 210 220 230 240 LVRSGSTESR GGKDPLSSPG GPGSRRSNYN LEGISVKMFL RGRPITMYIP SGIRSLEELP 250 260 270 280 290 300 SGPPPETLSL DWVYGYRGRD SRSNLFVLRS GEVVYFIACV VVLYRPGGGP GGPGGGGQRH 310 320 330 340 350 360 YRGHTDCVRC LAVHPDGVRV ASGQTAGVDK DGKPLQPVVH IWDSETLLKL QEIGLGAFER 370 380 390 400 410 420 GVGALAFSAA DQGAFLCVVD DSNEHMLSVW DCSRGMKLAE IKSTNDSVLA VGFNPRDSSC 430 440 450 460 470 480 IVTSGKSHVH FWNWSGGVGV PGNGTLTRKQ GVFGKYKKPK FIPCFVFLPD GDILTGDSEG 490 500 510 520 530 540 NILTWGRSPS DSKTPGRGGA KETYGIVAQA HAHEGSIFAL CLRRDGTVLS GGGRDRRLVQ 550 560 570 580 590 600 WGPGLVALQE AEIPEHFGAV RAIAEGLGSE LLVGTTKNAL LRGDLAQGFS PVIQGHTDEL 610 620 630 640 650 660 WGLCTHPSQN RFLTCGHDRQ LCLWDGESHA LAWSIDLKET GLCADFHPSG AVVAVGLNTG 670 680 690 700 710 720 RWLVLDTETR EIVSDVIDGN EQLSVVRYSP DGLYLAIGSH DNVIYIYSVS SDGAKSSRFG 730 740 750 760 770 780 RCMGHSSFIT HLDWSKDGNF IMSNSGDYEI LYWDVAGGCK QLKNRYESRD REWATYTCVL 790 800 810 820 830 840 GFHVYVPVRS CQGAEPHVRG PRQPRDQRPI HARRLAPRLA GRQGRQHLPV ASAGRWGRGA 850 860 870 880 890 900 GARHALSNPL PVPRLLPRRL IAAWRDRLAR RRGPAPPCPS LAQSPTTRGR LFPGLTSRHS 910 RSRIFLEGAN GAPAHTV
	Isoform 3 [UniParc]. Checksum: FBA484EF88EBA319	FASTA	194	20,224
	10 20 30 40 50 60 MELVKAALAE ALRLLRLQVP PSSLQGSGTP APPGDSLAAP PGLPPTCTPS LVSRGTQTET 70 80 90 100 110 120 EVELKSSPGP PGLSNGPPAP QGASEEPSGT QSEGGGSSSS GAGSPGPPGI LRPLQPPQRA 130 140 150 160 170 180 DTAASAEALQ EGNLLRQPVS AVREHREPWG KRPPLQPWGP WISEEQLQFG RHLSEDVPSR 190 APHYHVHPVW HPQP

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-896 (ISOFORM 2). Tissue: Testis and Uterus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus.
[3]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176 AND THR-881, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[4]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK093146 mRNA. Translation: BAC04073.1. Different initiation. AK128679 mRNA. Translation: BAC87566.1. Sequence problems. BC108280 mRNA. Translation: AAI08281.1.
IPI	IPI00167909. IPI00843902. IPI00843958.
RefSeq	NP_694997.2. NM_153265.2.
UniGene	Hs.379785.
3D structure databases
ProteinModelPortal	Q32P44.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q32P44. 3 interactions.
MINT	MINT-1682086.
STRING	9606.ENSP00000378254.
PTM databases
PhosphoSite	Q32P44.
Polymorphism databases
DMDM	121945710.
Proteomic databases
PaxDb	Q32P44.
PeptideAtlas	Q32P44.
PRIDE	Q32P44.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000394773; ENSP00000378254; ENSG00000149499. ENST00000494448; ENSP00000431752; ENSG00000149499. ENST00000529309; ENSP00000434513; ENSG00000149499.
GeneID	256364.
KEGG	hsa:256364.
UCSC	uc001ntr.1. human. uc001ntt.1. human.
Organism-specific databases
CTD	256364.
GeneCards	GC11M062370.
HGNC	HGNC:26666. EML3.
neXtProt	NX_Q32P44.
PharmGKB	PA142671910.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2319.
HOGENOM	HOG000294061.
HOVERGEN	HBG051470.
OMA	EMELVKA.
OrthoDB	EOG45755Z.
Enzyme and pathway databases
SignaLink	Q32P44.
Gene expression databases
ArrayExpress	Q32P44.
Bgee	Q32P44.
CleanEx	HS_EML3.
Genevestigator	Q32P44.
Family and domain databases
Gene3D	2.130.10.10. 3 hits.
InterPro	IPR005108. HELP. IPR011047. Quinonprotein_ADH-like_supfam. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF03451. HELP. 1 hit. PF00400. WD40. 5 hits. [Graphical view]
SMART	SM00320. WD40. 8 hits. [Graphical view]
SUPFAM	SSF50998. Quin_alc_DH_like. 1 hit. SSF50978. WD40_like. 2 hits.
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. 3 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	EML3. human.
GenomeRNAi	256364.
NextBio	92788.

Entry information

Entry name

EMAL3_HUMAN

Accession

Primary (citable) accession number: Q32P44
Secondary accession number(s): Q6ZQW7, Q8NA55

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	December 6, 2005
Last modified:	May 29, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.