Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q32P44

- EMAL3_HUMAN

UniProt

Q32P44 - EMAL3_HUMAN

Protein

Echinoderm microtubule-associated protein-like 3

Gene

EML3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (06 Dec 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.By similarity

    Enzyme and pathway databases

    SignaLinkiQ32P44.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Echinoderm microtubule-associated protein-like 3
    Short name:
    EMAP-3
    Gene namesi
    Name:EML3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:26666. EML3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. microtubule Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142671910.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 896896Echinoderm microtubule-associated protein-like 3PRO_0000284390Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei176 – 1761Phosphoserine5 Publications
    Modified residuei881 – 8811Phosphothreonine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ32P44.
    PaxDbiQ32P44.
    PeptideAtlasiQ32P44.
    PRIDEiQ32P44.

    PTM databases

    PhosphoSiteiQ32P44.

    Expressioni

    Gene expression databases

    ArrayExpressiQ32P44.
    BgeeiQ32P44.
    CleanExiHS_EML3.
    GenevestigatoriQ32P44.

    Interactioni

    Protein-protein interaction databases

    BioGridi129161. 6 interactions.
    IntActiQ32P44. 3 interactions.
    MINTiMINT-1682086.
    STRINGi9606.ENSP00000378254.

    Structurei

    3D structure databases

    ProteinModelPortaliQ32P44.
    SMRiQ32P44. Positions 361-392, 501-869.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati303 – 35250WD 1Add
    BLAST
    Repeati357 – 40044WD 2Add
    BLAST
    Repeati403 – 44240WD 3Add
    BLAST
    Repeati457 – 49539WD 4Add
    BLAST
    Repeati512 – 55140WD 5Add
    BLAST
    Repeati595 – 63440WD 6Add
    BLAST
    Repeati678 – 71740WD 7Add
    BLAST
    Repeati724 – 76340WD 8Add
    BLAST
    Repeati837 – 87741WD 9Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi126 – 1294Poly-Ser
    Compositional biasi156 – 1638Poly-Ser
    Compositional biasi287 – 29711Poly-GlyAdd
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat EMAP family.Curated
    Contains 9 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    HOGENOMiHOG000294061.
    HOVERGENiHBG051470.
    OMAiKAPSRMY.
    OrthoDBiEOG7XWPMW.
    PhylomeDBiQ32P44.
    TreeFamiTF317832.

    Family and domain databases

    Gene3Di2.130.10.10. 3 hits.
    InterProiIPR005108. HELP.
    IPR011047. Quinonprotein_ADH-like_supfam.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF03451. HELP. 1 hit.
    PF00400. WD40. 5 hits.
    [Graphical view]
    SMARTiSM00320. WD40. 8 hits.
    [Graphical view]
    SUPFAMiSSF50998. SSF50998. 3 hits.
    PROSITEiPS50082. WD_REPEATS_2. 3 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q32P44-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDGAAGPGDG PAREALQSLS QRLRVQEQEM ELVKAALAEA LRLLRLQVPP    50
    SSLQGSGTPA PPGDSLAAPP GLPPTCTPSL VSRGTQTETE VELKSSPGPP 100
    GLSNGPPAPQ GASEEPSGTQ SEGGGSSSSG AGSPGPPGIL RPLQPPQRAD 150
    TPRRNSSSSS SPSERPRQKL SRKAISSANL LVRSGSTESR GGKDPLSSPG 200
    GPGSRRSNYN LEGISVKMFL RGRPITMYIP SGIRSLEELP SGPPPETLSL 250
    DWVYGYRGRD SRSNLFVLRS GEVVYFIACV VVLYRPGGGP GGPGGGGQRH 300
    YRGHTDCVRC LAVHPDGVRV ASGQTAGVDK DGKPLQPVVH IWDSETLLKL 350
    QEIGLGAFER GVGALAFSAA DQGAFLCVVD DSNEHMLSVW DCSRGMKLAE 400
    IKSTNDSVLA VGFNPRDSSC IVTSGKSHVH FWNWSGGVGV PGNGTLTRKQ 450
    GVFGKYKKPK FIPCFVFLPD GDILTGDSEG NILTWGRSPS DSKTPGRGGA 500
    KETYGIVAQA HAHEGSIFAL CLRRDGTVLS GGGRDRRLVQ WGPGLVALQE 550
    AEIPEHFGAV RAIAEGLGSE LLVGTTKNAL LRGDLAQGFS PVIQGHTDEL 600
    WGLCTHPSQN RFLTCGHDRQ LCLWDGESHA LAWSIDLKET GLCADFHPSG 650
    AVVAVGLNTG RWLVLDTETR EIVSDVIDGN EQLSVVRYSP DGLYLAIGSH 700
    DNVIYIYSVS SDGAKSSRFG RCMGHSSFIT HLDWSKDGNF IMSNSGDYEI 750
    LYWDVAGGCK QLKNRYESRD REWATYTCVL GFHVYGVWPD GSDGTDINSL 800
    CRSHNERVVA VADDFCKVHL FQYPCARAKA PSRMYGGHGS HVTSVRFTHD 850
    DSHLVSLGGK DASIFQWRVL GAGGAGPAPA TPSRTPSLSP ASSLDV 896
    Length:896
    Mass (Da):95,197
    Last modified:December 6, 2005 - v1
    Checksum:i21D2EBD29B969D34
    GO
    Isoform 2 (identifier: Q32P44-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         786-896: GVWPDGSDGT...SLSPASSLDV → VPVRSCQGAE...GANGAPAHTV

    Note: No experimental confirmation available.

    Show »
    Length:917
    Mass (Da):97,987
    Checksum:iC5E4325DCE7C226F
    GO
    Isoform 3 (identifier: Q32P44-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-29: Missing.
         152-223: PRRNSSSSSS...ISVKMFLRGR → AASAEALQEG...HVHPVWHPQP
         224-896: Missing.

    Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

    Show »
    Length:194
    Mass (Da):20,224
    Checksum:iFBA484EF88EBA319
    GO

    Sequence cautioni

    The sequence BAC04073.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti65 – 651S → SS in BAC04073. (PubMed:14702039)Curated
    Sequence conflicti381 – 3811D → N in BAC04073. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti620 – 6201Q → K.
    Corresponds to variant rs34098002 [ dbSNP | Ensembl ].
    VAR_031725
    Isoform 2 (identifier: Q32P44-2)
    Natural varianti788 – 7881V → M.
    Corresponds to variant rs34709729 [ dbSNP | Ensembl ].

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2929Missing in isoform 3. 1 PublicationVSP_039926Add
    BLAST
    Alternative sequencei152 – 22372PRRNS…FLRGR → AASAEALQEGNLLRQPVSAV REHREPWGKRPPLQPWGPWI SEEQLQFGRHLSEDVPSRAP HYHVHPVWHPQP in isoform 3. 1 PublicationVSP_039927Add
    BLAST
    Alternative sequencei224 – 896673Missing in isoform 3. 1 PublicationVSP_039928Add
    BLAST
    Alternative sequencei786 – 896111GVWPD…SSLDV → VPVRSCQGAEPHVRGPRQPR DQRPIHARRLAPRLAGRQGR QHLPVASAGRWGRGAGARHA LSNPLPVPRLLPRRLIAAWR DRLARRRGPAPPCPSLAQSP TTRGRLFPGLTSRHSRSRIF LEGANGAPAHTV in isoform 2. 1 PublicationVSP_024483Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK093146 mRNA. Translation: BAC04073.1. Different initiation.
    AK128679 mRNA. Translation: BAC87566.1. Sequence problems.
    BC108280 mRNA. Translation: AAI08281.1.
    CCDSiCCDS8023.2. [Q32P44-1]
    RefSeqiNP_694997.2. NM_153265.2. [Q32P44-1]
    XP_005273934.1. XM_005273877.2. [Q32P44-2]
    UniGeneiHs.379785.

    Genome annotation databases

    EnsembliENST00000394773; ENSP00000378254; ENSG00000149499. [Q32P44-1]
    ENST00000494448; ENSP00000431752; ENSG00000149499. [Q32P44-3]
    ENST00000529309; ENSP00000434513; ENSG00000149499. [Q32P44-2]
    GeneIDi256364.
    KEGGihsa:256364.
    UCSCiuc001ntt.1. human. [Q32P44-1]
    uc010rly.1. human. [Q32P44-2]

    Polymorphism databases

    DMDMi121945710.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK093146 mRNA. Translation: BAC04073.1 . Different initiation.
    AK128679 mRNA. Translation: BAC87566.1 . Sequence problems.
    BC108280 mRNA. Translation: AAI08281.1 .
    CCDSi CCDS8023.2. [Q32P44-1 ]
    RefSeqi NP_694997.2. NM_153265.2. [Q32P44-1 ]
    XP_005273934.1. XM_005273877.2. [Q32P44-2 ]
    UniGenei Hs.379785.

    3D structure databases

    ProteinModelPortali Q32P44.
    SMRi Q32P44. Positions 361-392, 501-869.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 129161. 6 interactions.
    IntActi Q32P44. 3 interactions.
    MINTi MINT-1682086.
    STRINGi 9606.ENSP00000378254.

    PTM databases

    PhosphoSitei Q32P44.

    Polymorphism databases

    DMDMi 121945710.

    Proteomic databases

    MaxQBi Q32P44.
    PaxDbi Q32P44.
    PeptideAtlasi Q32P44.
    PRIDEi Q32P44.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000394773 ; ENSP00000378254 ; ENSG00000149499 . [Q32P44-1 ]
    ENST00000494448 ; ENSP00000431752 ; ENSG00000149499 . [Q32P44-3 ]
    ENST00000529309 ; ENSP00000434513 ; ENSG00000149499 . [Q32P44-2 ]
    GeneIDi 256364.
    KEGGi hsa:256364.
    UCSCi uc001ntt.1. human. [Q32P44-1 ]
    uc010rly.1. human. [Q32P44-2 ]

    Organism-specific databases

    CTDi 256364.
    GeneCardsi GC11M062370.
    HGNCi HGNC:26666. EML3.
    neXtProti NX_Q32P44.
    PharmGKBi PA142671910.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2319.
    HOGENOMi HOG000294061.
    HOVERGENi HBG051470.
    OMAi KAPSRMY.
    OrthoDBi EOG7XWPMW.
    PhylomeDBi Q32P44.
    TreeFami TF317832.

    Enzyme and pathway databases

    SignaLinki Q32P44.

    Miscellaneous databases

    ChiTaRSi EML3. human.
    GenomeRNAii 256364.
    NextBioi 92788.
    PROi Q32P44.

    Gene expression databases

    ArrayExpressi Q32P44.
    Bgeei Q32P44.
    CleanExi HS_EML3.
    Genevestigatori Q32P44.

    Family and domain databases

    Gene3Di 2.130.10.10. 3 hits.
    InterProi IPR005108. HELP.
    IPR011047. Quinonprotein_ADH-like_supfam.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF03451. HELP. 1 hit.
    PF00400. WD40. 5 hits.
    [Graphical view ]
    SMARTi SM00320. WD40. 8 hits.
    [Graphical view ]
    SUPFAMi SSF50998. SSF50998. 3 hits.
    PROSITEi PS50082. WD_REPEATS_2. 3 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-896 (ISOFORM 2).
      Tissue: Testis and Uterus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Uterus.
    3. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
      Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
      Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176 AND THR-881, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    4. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
      Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
      J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiEMAL3_HUMAN
    AccessioniPrimary (citable) accession number: Q32P44
    Secondary accession number(s): Q6ZQW7, Q8NA55
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 17, 2007
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3