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Q32P41

- TRM5_HUMAN

UniProt

Q32P41 - TRM5_HUMAN

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Protein

tRNA (guanine(37)-N1)-methyltransferase

Gene

TRMT5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.1 PublicationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei289 – 2891S-adenosyl-L-methionineUniRule annotation
Binding sitei387 – 3871S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine(37)-N(1))-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

SABIO-RKQ32P41.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(37)-N1)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
tRNA methyltransferase 5 homologUniRule annotation
Gene namesi
Name:TRMT5UniRule annotation
Synonyms:KIAA1393, TRM5UniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 14

Organism-specific databases

HGNCiHGNC:23141. TRMT5.

Subcellular locationi

Mitochondrion matrix UniRule annotation. Nucleus UniRule annotation. Cytoplasm UniRule annotation
Note: Predominantly in the mitochondria and in the nucleus.UniRule annotation

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-HAMAP
  2. nucleus Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134952106.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 509509tRNA (guanine(37)-N1)-methyltransferasePRO_0000256513Add
BLAST

Proteomic databases

MaxQBiQ32P41.
PaxDbiQ32P41.
PRIDEiQ32P41.

PTM databases

PhosphoSiteiQ32P41.

Expressioni

Gene expression databases

BgeeiQ32P41.
CleanExiHS_TRMT5.
ExpressionAtlasiQ32P41. baseline and differential.
GenevestigatoriQ32P41.

Organism-specific databases

HPAiHPA000943.

Interactioni

Subunit structurei

Monomer.1 PublicationUniRule annotation

Protein-protein interaction databases

BioGridi121623. 1 interaction.
STRINGi9606.ENSP00000261249.

Structurei

3D structure databases

ProteinModelPortaliQ32P41.
SMRiQ32P41. Positions 184-468.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni327 – 3282S-adenosyl-L-methionine bindingUniRule annotation
Regioni355 – 3562S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2520.
GeneTreeiENSGT00390000008208.
HOGENOMiHOG000195270.
HOVERGENiHBG094093.
InParanoidiQ32P41.
KOiK15429.
OMAiPVAKKNC.
OrthoDBiEOG7VDXP0.
PhylomeDBiQ32P41.
TreeFamiTF315073.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03152. TRM5.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025792. tRNA_Gua_MeTrfase_euk.
IPR003402. tRNA_Trfase_Trm5/Tyw2.
[Graphical view]
PfamiPF02475. Met_10. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q32P41-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVLWILWRPF GFSGRFLKLE SHSITESKSL IPVAWTSLTQ MLLEAPGIFL
60 70 80 90 100
LGQRKRFSTM PETETHERET ELFSPPSDVR GMTKLDRTAF KKTVNIPVLK
110 120 130 140 150
VRKEIVSKLM RSLKRAALQR PGIRRVIEDP EDKESRLIML DPYKIFTHDS
160 170 180 190 200
FEKAELSVLE QLNVSPQISK YNLELTYEHF KSEEILRAVL PEGQDVTSGF
210 220 230 240 250
SRIGHIAHLN LRDHQLSFKH LIGQVMIDKN PGITSAVNKI NNIDNMYRNF
260 270 280 290 300
QMEVLSGEQN MMTKVRENNY TYEFDFSKVY WNPRLSTEHS RITELLKPGD
310 320 330 340 350
VLFDVFAGVG PFAIPVAKKN CTVFANDLNP ESHKWLLYNC KLNKVDQKVK
360 370 380 390 400
VFNLDGKDFL QGPVKEELMQ LLGLSKERKP SVHVVMNLPA KAIEFLSAFK
410 420 430 440 450
WLLDGQPCSS EFLPIVHCYS FSKDANPAED VRQRAGAVLG ISLEACSSVH
460 470 480 490 500
LVRNVAPNKE MLCITFQIPA SVLYKNQTRN PENHEDPPLK RQRTAEAFSD

EKTQIVSNT
Length:509
Mass (Da):58,246
Last modified:April 17, 2007 - v2
Checksum:i4BCF6A00EBACD4FB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti394 – 3941E → K in BAA92631. (PubMed:10718198)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti217 – 2171S → P.4 Publications
Corresponds to variant rs7142228 [ dbSNP | Ensembl ].
VAR_028898
Natural varianti255 – 2551L → P.
Corresponds to variant rs2882686 [ dbSNP | Ensembl ].
VAR_028899
Natural varianti294 – 2941E → A.
Corresponds to variant rs2296928 [ dbSNP | Ensembl ].
VAR_028900

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK293158 mRNA. Translation: BAG56703.1.
AL160236 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW80792.1.
BC108284 mRNA. Translation: AAI08285.1.
BC136606 mRNA. Translation: AAI36607.1.
BC136607 mRNA. Translation: AAI36608.1.
AB037814 mRNA. Translation: BAA92631.1.
CCDSiCCDS32092.1.
RefSeqiNP_065861.3. NM_020810.3.
UniGeneiHs.380159.

Genome annotation databases

EnsembliENST00000261249; ENSP00000261249; ENSG00000126814.
GeneIDi57570.
KEGGihsa:57570.
UCSCiuc001xff.4. human.

Polymorphism databases

DMDMi145559536.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK293158 mRNA. Translation: BAG56703.1 .
AL160236 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW80792.1 .
BC108284 mRNA. Translation: AAI08285.1 .
BC136606 mRNA. Translation: AAI36607.1 .
BC136607 mRNA. Translation: AAI36608.1 .
AB037814 mRNA. Translation: BAA92631.1 .
CCDSi CCDS32092.1.
RefSeqi NP_065861.3. NM_020810.3.
UniGenei Hs.380159.

3D structure databases

ProteinModelPortali Q32P41.
SMRi Q32P41. Positions 184-468.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121623. 1 interaction.
STRINGi 9606.ENSP00000261249.

PTM databases

PhosphoSitei Q32P41.

Polymorphism databases

DMDMi 145559536.

Proteomic databases

MaxQBi Q32P41.
PaxDbi Q32P41.
PRIDEi Q32P41.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000261249 ; ENSP00000261249 ; ENSG00000126814 .
GeneIDi 57570.
KEGGi hsa:57570.
UCSCi uc001xff.4. human.

Organism-specific databases

CTDi 57570.
GeneCardsi GC14M061438.
H-InvDB HIX0011715.
HGNCi HGNC:23141. TRMT5.
HPAi HPA000943.
MIMi 611023. gene.
neXtProti NX_Q32P41.
PharmGKBi PA134952106.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG2520.
GeneTreei ENSGT00390000008208.
HOGENOMi HOG000195270.
HOVERGENi HBG094093.
InParanoidi Q32P41.
KOi K15429.
OMAi PVAKKNC.
OrthoDBi EOG7VDXP0.
PhylomeDBi Q32P41.
TreeFami TF315073.

Enzyme and pathway databases

SABIO-RK Q32P41.

Miscellaneous databases

ChiTaRSi TRMT5. human.
GenomeRNAii 57570.
NextBioi 64090.
PROi Q32P41.
SOURCEi Search...

Gene expression databases

Bgeei Q32P41.
CleanExi HS_TRMT5.
ExpressionAtlasi Q32P41. baseline and differential.
Genevestigatori Q32P41.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03152. TRM5.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025792. tRNA_Gua_MeTrfase_euk.
IPR003402. tRNA_Trfase_Trm5/Tyw2.
[Graphical view ]
Pfami PF02475. Met_10. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-217.
  2. "The DNA sequence and analysis of human chromosome 14."
    Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
    , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
    Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT PRO-217.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-217.
    Tissue: Bone.
  5. "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
    DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 10-509, VARIANT PRO-217.
    Tissue: Brain.
  6. "Isolation and characterization of the human tRNA-(N1G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD protein."
    Brule H., Elliott M., Redlak M., Zehner Z.E., Holmes W.M.
    Biochemistry 43:9243-9255(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, SUBUNIT.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTRM5_HUMAN
AccessioniPrimary (citable) accession number: Q32P41
Secondary accession number(s): B2RN19, Q9P2F4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: April 17, 2007
Last modified: October 29, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3