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Q32P41

- TRM5_HUMAN

UniProt

Q32P41 - TRM5_HUMAN

Protein

tRNA (guanine(37)-N1)-methyltransferase

Gene

TRMT5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (17 Apr 2007)
      Previous versions | rss
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    Functioni

    Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.1 PublicationUniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei289 – 2891S-adenosyl-L-methionineUniRule annotation
    Binding sitei387 – 3871S-adenosyl-L-methionineUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine(37)-N(1))-methyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    SABIO-RKQ32P41.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine(37)-N1)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
    Alternative name(s):
    M1G-methyltransferaseUniRule annotation
    tRNA [GM37] methyltransferaseUniRule annotation
    tRNA methyltransferase 5 homologUniRule annotation
    Gene namesi
    Name:TRMT5UniRule annotation
    Synonyms:KIAA1393, TRM5UniRule annotation
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:23141. TRMT5.

    Subcellular locationi

    Mitochondrion matrix UniRule annotation. Nucleus UniRule annotation. Cytoplasm UniRule annotation
    Note: Predominantly in the mitochondria and in the nucleus.UniRule annotation

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134952106.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 509509tRNA (guanine(37)-N1)-methyltransferasePRO_0000256513Add
    BLAST

    Proteomic databases

    MaxQBiQ32P41.
    PaxDbiQ32P41.
    PRIDEiQ32P41.

    PTM databases

    PhosphoSiteiQ32P41.

    Expressioni

    Gene expression databases

    ArrayExpressiQ32P41.
    BgeeiQ32P41.
    CleanExiHS_TRMT5.
    GenevestigatoriQ32P41.

    Organism-specific databases

    HPAiHPA000943.

    Interactioni

    Subunit structurei

    Monomer.1 PublicationUniRule annotation

    Protein-protein interaction databases

    BioGridi121623. 1 interaction.
    STRINGi9606.ENSP00000261249.

    Structurei

    3D structure databases

    ProteinModelPortaliQ32P41.
    SMRiQ32P41. Positions 184-468.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni327 – 3282S-adenosyl-L-methionine bindingUniRule annotation
    Regioni355 – 3562S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2520.
    HOGENOMiHOG000195270.
    HOVERGENiHBG094093.
    InParanoidiQ32P41.
    KOiK15429.
    OMAiPVAKKNC.
    OrthoDBiEOG7VDXP0.
    PhylomeDBiQ32P41.
    TreeFamiTF315073.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03152. TRM5.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025792. tRNA_Gua_MeTrfase_euk.
    IPR003402. tRNA_Trfase_Trm5/Tyw2.
    [Graphical view]
    PfamiPF02475. Met_10. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51684. SAM_MT_TRM5_TYW2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q32P41-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVLWILWRPF GFSGRFLKLE SHSITESKSL IPVAWTSLTQ MLLEAPGIFL    50
    LGQRKRFSTM PETETHERET ELFSPPSDVR GMTKLDRTAF KKTVNIPVLK 100
    VRKEIVSKLM RSLKRAALQR PGIRRVIEDP EDKESRLIML DPYKIFTHDS 150
    FEKAELSVLE QLNVSPQISK YNLELTYEHF KSEEILRAVL PEGQDVTSGF 200
    SRIGHIAHLN LRDHQLSFKH LIGQVMIDKN PGITSAVNKI NNIDNMYRNF 250
    QMEVLSGEQN MMTKVRENNY TYEFDFSKVY WNPRLSTEHS RITELLKPGD 300
    VLFDVFAGVG PFAIPVAKKN CTVFANDLNP ESHKWLLYNC KLNKVDQKVK 350
    VFNLDGKDFL QGPVKEELMQ LLGLSKERKP SVHVVMNLPA KAIEFLSAFK 400
    WLLDGQPCSS EFLPIVHCYS FSKDANPAED VRQRAGAVLG ISLEACSSVH 450
    LVRNVAPNKE MLCITFQIPA SVLYKNQTRN PENHEDPPLK RQRTAEAFSD 500
    EKTQIVSNT 509
    Length:509
    Mass (Da):58,246
    Last modified:April 17, 2007 - v2
    Checksum:i4BCF6A00EBACD4FB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti394 – 3941E → K in BAA92631. (PubMed:10718198)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti217 – 2171S → P.4 Publications
    Corresponds to variant rs7142228 [ dbSNP | Ensembl ].
    VAR_028898
    Natural varianti255 – 2551L → P.
    Corresponds to variant rs2882686 [ dbSNP | Ensembl ].
    VAR_028899
    Natural varianti294 – 2941E → A.
    Corresponds to variant rs2296928 [ dbSNP | Ensembl ].
    VAR_028900

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK293158 mRNA. Translation: BAG56703.1.
    AL160236 Genomic DNA. No translation available.
    CH471061 Genomic DNA. Translation: EAW80792.1.
    BC108284 mRNA. Translation: AAI08285.1.
    BC136606 mRNA. Translation: AAI36607.1.
    BC136607 mRNA. Translation: AAI36608.1.
    AB037814 mRNA. Translation: BAA92631.1.
    CCDSiCCDS32092.1.
    RefSeqiNP_065861.3. NM_020810.3.
    UniGeneiHs.380159.

    Genome annotation databases

    EnsembliENST00000261249; ENSP00000261249; ENSG00000126814.
    GeneIDi57570.
    KEGGihsa:57570.
    UCSCiuc001xff.4. human.

    Polymorphism databases

    DMDMi145559536.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK293158 mRNA. Translation: BAG56703.1 .
    AL160236 Genomic DNA. No translation available.
    CH471061 Genomic DNA. Translation: EAW80792.1 .
    BC108284 mRNA. Translation: AAI08285.1 .
    BC136606 mRNA. Translation: AAI36607.1 .
    BC136607 mRNA. Translation: AAI36608.1 .
    AB037814 mRNA. Translation: BAA92631.1 .
    CCDSi CCDS32092.1.
    RefSeqi NP_065861.3. NM_020810.3.
    UniGenei Hs.380159.

    3D structure databases

    ProteinModelPortali Q32P41.
    SMRi Q32P41. Positions 184-468.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 121623. 1 interaction.
    STRINGi 9606.ENSP00000261249.

    PTM databases

    PhosphoSitei Q32P41.

    Polymorphism databases

    DMDMi 145559536.

    Proteomic databases

    MaxQBi Q32P41.
    PaxDbi Q32P41.
    PRIDEi Q32P41.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000261249 ; ENSP00000261249 ; ENSG00000126814 .
    GeneIDi 57570.
    KEGGi hsa:57570.
    UCSCi uc001xff.4. human.

    Organism-specific databases

    CTDi 57570.
    GeneCardsi GC14M061438.
    H-InvDB HIX0011715.
    HGNCi HGNC:23141. TRMT5.
    HPAi HPA000943.
    MIMi 611023. gene.
    neXtProti NX_Q32P41.
    PharmGKBi PA134952106.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2520.
    HOGENOMi HOG000195270.
    HOVERGENi HBG094093.
    InParanoidi Q32P41.
    KOi K15429.
    OMAi PVAKKNC.
    OrthoDBi EOG7VDXP0.
    PhylomeDBi Q32P41.
    TreeFami TF315073.

    Enzyme and pathway databases

    SABIO-RK Q32P41.

    Miscellaneous databases

    ChiTaRSi TRMT5. human.
    GenomeRNAii 57570.
    NextBioi 64090.
    PROi Q32P41.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q32P41.
    Bgeei Q32P41.
    CleanExi HS_TRMT5.
    Genevestigatori Q32P41.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03152. TRM5.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025792. tRNA_Gua_MeTrfase_euk.
    IPR003402. tRNA_Trfase_Trm5/Tyw2.
    [Graphical view ]
    Pfami PF02475. Met_10. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51684. SAM_MT_TRM5_TYW2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-217.
    2. "The DNA sequence and analysis of human chromosome 14."
      Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
      , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
      Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT PRO-217.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-217.
      Tissue: Bone.
    5. "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
      DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 10-509, VARIANT PRO-217.
      Tissue: Brain.
    6. "Isolation and characterization of the human tRNA-(N1G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD protein."
      Brule H., Elliott M., Redlak M., Zehner Z.E., Holmes W.M.
      Biochemistry 43:9243-9255(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, SUBUNIT.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTRM5_HUMAN
    AccessioniPrimary (citable) accession number: Q32P41
    Secondary accession number(s): B2RN19, Q9P2F4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: April 17, 2007
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3