Q32P41 (TRM5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine(37)-N1)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
tRNA methyltransferase 5 homolog

Gene names

Name:	TRMT5
Synonyms:	KIAA1393, TRM5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding. HAMAP-Rule MF_03152
Catalytic activity	S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine37 in tRNA. Ref.6
Subunit structure	Monomer. Ref.6
Subcellular location	Mitochondrion matrix By similarity. Nucleus By similarity. Cytoplasm By similarity. Note: Predominantly in the mitochondria and in the nucleus By similarity.
Sequence similarities	Belongs to the TRM5 / TYW2 family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Mitochondrion Nucleus
Coding sequence diversity	Polymorphism
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine(37)-N(1))-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	tRNA (guanine(37)-N1)-methyltransferase HAMAP-Rule MF_03152		PRO_0000256513
Regions
	Region	327 – 328	2	S-adenosyl-L-methionine binding By similarity
	Region	355 – 356	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	289	1	S-adenosyl-L-methionine By similarity
	Binding site	387	1	S-adenosyl-L-methionine By similarity
Natural variations
	Natural variant	217	1	S → P. Ref.1 Ref.3 Ref.4 Ref.5 Corresponds to variant rs7142228 [ dbSNP | Ensembl ].		VAR_028898
	Natural variant	255	1	L → P. Corresponds to variant rs2882686 [ dbSNP | Ensembl ].		VAR_028899
	Natural variant	294	1	E → A. Corresponds to variant rs2296928 [ dbSNP | Ensembl ].		VAR_028900
Experimental info
	Sequence conflict	394	1	E → K in BAA92631. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q32P41 [UniParc]. Last modified April 17, 2007. Version 2. Checksum: 4BCF6A00EBACD4FB	FASTA	509	58,246
	10 20 30 40 50 60 MVLWILWRPF GFSGRFLKLE SHSITESKSL IPVAWTSLTQ MLLEAPGIFL LGQRKRFSTM 70 80 90 100 110 120 PETETHERET ELFSPPSDVR GMTKLDRTAF KKTVNIPVLK VRKEIVSKLM RSLKRAALQR 130 140 150 160 170 180 PGIRRVIEDP EDKESRLIML DPYKIFTHDS FEKAELSVLE QLNVSPQISK YNLELTYEHF 190 200 210 220 230 240 KSEEILRAVL PEGQDVTSGF SRIGHIAHLN LRDHQLSFKH LIGQVMIDKN PGITSAVNKI 250 260 270 280 290 300 NNIDNMYRNF QMEVLSGEQN MMTKVRENNY TYEFDFSKVY WNPRLSTEHS RITELLKPGD 310 320 330 340 350 360 VLFDVFAGVG PFAIPVAKKN CTVFANDLNP ESHKWLLYNC KLNKVDQKVK VFNLDGKDFL 370 380 390 400 410 420 QGPVKEELMQ LLGLSKERKP SVHVVMNLPA KAIEFLSAFK WLLDGQPCSS EFLPIVHCYS 430 440 450 460 470 480 FSKDANPAED VRQRAGAVLG ISLEACSSVH LVRNVAPNKE MLCITFQIPA SVLYKNQTRN 490 500 PENHEDPPLK RQRTAEAFSD EKTQIVSNT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-217.
[2]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT PRO-217.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-217. Tissue: Bone.
[5]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 10-509, VARIANT PRO-217. Tissue: Brain.
[6]	"Isolation and characterization of the human tRNA-(N1G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD protein." Brule H., Elliott M., Redlak M., Zehner Z.E., Holmes W.M. Biochemistry 43:9243-9255(2004) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, SUBUNIT.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK293158 mRNA. Translation: BAG56703.1. AL160236 Genomic DNA. No translation available. CH471061 Genomic DNA. Translation: EAW80792.1. BC108284 mRNA. Translation: AAI08285.1. BC136606 mRNA. Translation: AAI36607.1. BC136607 mRNA. Translation: AAI36608.1. AB037814 mRNA. Translation: BAA92631.1.
IPI	IPI00455268.
RefSeq	NP_065861.2. NM_020810.2.
UniGene	Hs.380159.
3D structure databases
ProteinModelPortal	Q32P41.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000261249.
PTM databases
PhosphoSite	Q32P41.
Polymorphism databases
DMDM	145559536.
Proteomic databases
PaxDb	Q32P41.
PRIDE	Q32P41.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000261249; ENSP00000261249; ENSG00000126814.
GeneID	57570.
KEGG	hsa:57570.
UCSC	uc001xff.4. human.
Organism-specific databases
CTD	57570.
GeneCards	GC14M061438.
H-InvDB	HIX0011715.
HGNC	HGNC:23141. TRMT5.
HPA	HPA000943.
MIM	611023. gene.
neXtProt	NX_Q32P41.
PharmGKB	PA134952106.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2520.
HOGENOM	HOG000195270.
HOVERGEN	HBG094093.
InParanoid	Q32P41.
KO	K15429.
OMA	GFSRVGH.
OrthoDB	EOG4W9J3N.
PhylomeDB	Q32P41.
Enzyme and pathway databases
SABIO-RK	Q32P41.
Gene expression databases
ArrayExpress	Q32P41.
Bgee	Q32P41.
CleanEx	HS_TRMT5.
Genevestigator	Q32P41.
GermOnline	ENSG00000126814. Homo sapiens.
Family and domain databases
HAMAP	MF_03152. TRM5.
InterPro	IPR025792. tRNA_Gua_MeTrfase_euk. IPR003402. tRNA_Trfase_Trm5/Tyw2. [Graphical view]
Pfam	PF02475. Met_10. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TRMT5. human.
GenomeRNAi	57570.
NextBio	64090.
SOURCE	Search...

Entry information

Entry name

TRM5_HUMAN

Accession

Primary (citable) accession number: Q32P41
Secondary accession number(s): B2RN19, Q9P2F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	April 17, 2007
Last modified:	May 1, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.