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Protein

Protein quaking-A

Gene

qki-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein that plays a central role in myelinization. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation. Required to protect and promote stability of mRNAs which promotes oligodendrocyte differentiation. Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of some pre-mRNA. Acts as a translational repressor (By similarity). Essential for notochord development.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, mRNA processing, mRNA splicing, mRNA transport, Translation regulation, Transport

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein quaking-A
Short name:
Xqua
Gene namesi
Name:qki-a
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6254729. qki.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341Protein quaking-APRO_0000239380Add
BLAST

Expressioni

Developmental stagei

First detected in the chordamesoderm of the dorsal blastopore lip of the mid-gastrula embryo (stage 11). This tissue, which differentiates into the notochord. In late gastrula embryos (stage 13), it is highly expressed in the notochord, and the original expression domain expands to include the tissue surrounding the blastopore. In neurula embryos, it is maintained in the notochord and the circumblastoporal region and now also extends to the paraxial mesoderm and the neuroectoderm. By the tailbud stage, it is expressed in various mesodermal and neural tissues. Highly expressed in the brain and the neural tube. Maintained throughout development to the tadpole stage (stage 35).2 Publications

Interactioni

Subunit structurei

Homodimer. Does not require RNA to homodimerize.1 Publication

Structurei

Secondary structure

1
341
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi15 – 2915Combined sources
Helixi34 – 374Combined sources
Helixi41 – 5414Combined sources
Beta strandi83 – 886Combined sources
Turni91 – 933Combined sources
Beta strandi95 – 973Combined sources
Helixi99 – 1035Combined sources
Turni104 – 1074Combined sources
Helixi108 – 11811Combined sources
Beta strandi119 – 1268Combined sources
Helixi133 – 1375Combined sources
Helixi143 – 1464Combined sources
Turni147 – 1493Combined sources
Beta strandi153 – 1586Combined sources
Helixi163 – 17917Combined sources
Beta strandi184 – 1885Combined sources
Helixi189 – 1957Combined sources
Helixi197 – 1993Combined sources
Beta strandi200 – 2023Combined sources
Turni203 – 2053Combined sources
Helixi207 – 2104Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BL5NMR-A82-215[»]
2YMJNMR-A/B8-57[»]
DisProtiDP00286.
ProteinModelPortaliQ32NN2.
SMRiQ32NN2. Positions 82-215.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ32NN2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini88 – 15467KHAdd
BLAST

Sequence similaritiesi

Contains 1 KH domain.Curated

Phylogenomic databases

HOVERGENiHBG093883.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR032367. Quaking_NLS.
IPR032377. STAR_dimer.
[Graphical view]
PfamiPF00013. KH_1. 1 hit.
PF16551. Quaking_NLS. 1 hit.
PF16544. STAR_dimer. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q32NN2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGEMETKEK PKPTPDYLMQ LMNDKKLMSS LPNFCGIFTH LERLLDEEIS
60 70 80 90 100
RVRKDMYNDT MNSSSNEKRT SELPDGIGPI VQLQEKLYVP VKEYPDFNFV
110 120 130 140 150
GRILGPRGLT AKQLEAETGC KIMVRGKGSM RDKKKEEQNR GKPNWEHLNE
160 170 180 190 200
DLHVLITVED AQNRAELKLK RAVEEVKKLL VPAAEGEDSL KKMQLMELAI
210 220 230 240 250
LNGTYRDANL KSPALAFSLA ATGQAPRIIT GPAPVLSPAA LRTPTPAGHT
260 270 280 290 300
LMPLIRQIQT AVMPNGTPHP TATLMQQAPE GGLIYTPYEY PYTLAPATSI
310 320 330 340
LEYPIEASGV LGAVATKVRR HDMRVHPYQR IVTADRAATG N
Length:341
Mass (Da):37,879
Last modified:June 13, 2006 - v2
Checksum:iF39F36CB80DE77A1
GO
Isoform 2 (identifier: Q32NN2-2) [UniParc]FASTAAdd to basket
Also known as: Xqua365

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MFFGHAGCDIRNSAAPAFGLFDWNM

Show »
Length:365
Mass (Da):40,506
Checksum:i9807D264199D0265
GO
Isoform 3 (identifier: Q32NN2-3) [UniParc]FASTAAdd to basket
Also known as: Xqua357

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MFFGHAGCDIRNSAAPAFGLFDWNM
     214-221: Missing.

Show »
Length:357
Mass (Da):39,761
Checksum:iFFD06B64C1557588
GO
Isoform 4 (identifier: Q32NN2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     214-221: Missing.

Show »
Length:333
Mass (Da):37,134
Checksum:iFA74544BCED95754
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti194 – 1941Q → K (PubMed:9133592).Curated
Sequence conflicti194 – 1941Q → K (PubMed:15811367).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MFFGHAGCDIRNSAAPAFGL FDWNM in isoform 2 and isoform 3. 1 PublicationVSP_019206
Alternative sequencei214 – 2218Missing in isoform 3 and isoform 4. 1 PublicationVSP_019207

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC108554 mRNA. Translation: AAI08555.1.
RefSeqiNP_001089857.1. NM_001096388.1. [Q32NN2-4]
UniGeneiXl.55586.

Genome annotation databases

GeneIDi734923.
KEGGixla:734923.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC108554 mRNA. Translation: AAI08555.1.
RefSeqiNP_001089857.1. NM_001096388.1. [Q32NN2-4]
UniGeneiXl.55586.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BL5NMR-A82-215[»]
2YMJNMR-A/B8-57[»]
DisProtiDP00286.
ProteinModelPortaliQ32NN2.
SMRiQ32NN2. Positions 82-215.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi734923.
KEGGixla:734923.

Organism-specific databases

CTDi734923.
XenbaseiXB-GENE-6254729. qki.

Phylogenomic databases

HOVERGENiHBG093883.

Miscellaneous databases

EvolutionaryTraceiQ32NN2.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR032367. Quaking_NLS.
IPR032377. STAR_dimer.
[Graphical view]
PfamiPF00013. KH_1. 1 hit.
PF16551. Quaking_NLS. 1 hit.
PF16544. STAR_dimer. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQKIA_XENLA
AccessioniPrimary (citable) accession number: Q32NN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: July 6, 2016
This is version 52 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.