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Protein

Protein quaking-A

Gene

qki-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein that plays a central role in myelinization. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation. Required to protect and promote stability of mRNAs which promotes oligodendrocyte differentiation. Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of some pre-mRNA. Acts as a translational repressor (By similarity). Essential for notochord development.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, mRNA processing, mRNA splicing, mRNA transport, Translation regulation, Transport

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein quaking-A
Short name:
Xqua
Gene namesi
Name:qki-a
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6254729. qki.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002393801 – 341Protein quaking-AAdd BLAST341

Proteomic databases

PRIDEiQ32NN2.

Expressioni

Developmental stagei

First detected in the chordamesoderm of the dorsal blastopore lip of the mid-gastrula embryo (stage 11). This tissue, which differentiates into the notochord. In late gastrula embryos (stage 13), it is highly expressed in the notochord, and the original expression domain expands to include the tissue surrounding the blastopore. In neurula embryos, it is maintained in the notochord and the circumblastoporal region and now also extends to the paraxial mesoderm and the neuroectoderm. By the tailbud stage, it is expressed in various mesodermal and neural tissues. Highly expressed in the brain and the neural tube. Maintained throughout development to the tadpole stage (stage 35).2 Publications

Interactioni

Subunit structurei

Homodimer. Does not require RNA to homodimerize.1 Publication

Structurei

Secondary structure

1341
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 29Combined sources15
Helixi34 – 37Combined sources4
Helixi41 – 54Combined sources14
Beta strandi83 – 88Combined sources6
Turni91 – 93Combined sources3
Beta strandi95 – 97Combined sources3
Helixi99 – 103Combined sources5
Turni104 – 107Combined sources4
Helixi108 – 118Combined sources11
Beta strandi119 – 126Combined sources8
Helixi133 – 137Combined sources5
Helixi143 – 146Combined sources4
Turni147 – 149Combined sources3
Beta strandi153 – 158Combined sources6
Helixi163 – 179Combined sources17
Beta strandi184 – 188Combined sources5
Helixi189 – 195Combined sources7
Helixi197 – 199Combined sources3
Beta strandi200 – 202Combined sources3
Turni203 – 205Combined sources3
Helixi207 – 210Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BL5NMR-A82-215[»]
2YMJNMR-A/B8-57[»]
DisProtiDP00286.
ProteinModelPortaliQ32NN2.
SMRiQ32NN2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ32NN2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 154KHAdd BLAST67

Sequence similaritiesi

Contains 1 KH domain.Curated

Phylogenomic databases

HOVERGENiHBG093883.
KOiK14945.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR032367. Quaking_NLS.
IPR032377. STAR_dimer.
[Graphical view]
PfamiPF00013. KH_1. 1 hit.
PF16551. Quaking_NLS. 1 hit.
PF16544. STAR_dimer. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q32NN2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGEMETKEK PKPTPDYLMQ LMNDKKLMSS LPNFCGIFTH LERLLDEEIS
60 70 80 90 100
RVRKDMYNDT MNSSSNEKRT SELPDGIGPI VQLQEKLYVP VKEYPDFNFV
110 120 130 140 150
GRILGPRGLT AKQLEAETGC KIMVRGKGSM RDKKKEEQNR GKPNWEHLNE
160 170 180 190 200
DLHVLITVED AQNRAELKLK RAVEEVKKLL VPAAEGEDSL KKMQLMELAI
210 220 230 240 250
LNGTYRDANL KSPALAFSLA ATGQAPRIIT GPAPVLSPAA LRTPTPAGHT
260 270 280 290 300
LMPLIRQIQT AVMPNGTPHP TATLMQQAPE GGLIYTPYEY PYTLAPATSI
310 320 330 340
LEYPIEASGV LGAVATKVRR HDMRVHPYQR IVTADRAATG N
Length:341
Mass (Da):37,879
Last modified:June 13, 2006 - v2
Checksum:iF39F36CB80DE77A1
GO
Isoform 2 (identifier: Q32NN2-2) [UniParc]FASTAAdd to basket
Also known as: Xqua365

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MFFGHAGCDIRNSAAPAFGLFDWNM

Show »
Length:365
Mass (Da):40,506
Checksum:i9807D264199D0265
GO
Isoform 3 (identifier: Q32NN2-3) [UniParc]FASTAAdd to basket
Also known as: Xqua357

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MFFGHAGCDIRNSAAPAFGLFDWNM
     214-221: Missing.

Show »
Length:357
Mass (Da):39,761
Checksum:iFFD06B64C1557588
GO
Isoform 4 (identifier: Q32NN2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     214-221: Missing.

Show »
Length:333
Mass (Da):37,134
Checksum:iFA74544BCED95754
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti194Q → K (PubMed:9133592).Curated1
Sequence conflicti194Q → K (PubMed:15811367).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0192061M → MFFGHAGCDIRNSAAPAFGL FDWNM in isoform 2 and isoform 3. 1 Publication1
Alternative sequenceiVSP_019207214 – 221Missing in isoform 3 and isoform 4. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC108554 mRNA. Translation: AAI08555.1.
RefSeqiNP_001089857.1. NM_001096388.1. [Q32NN2-4]
UniGeneiXl.55586.

Genome annotation databases

GeneIDi734923.
KEGGixla:734923.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC108554 mRNA. Translation: AAI08555.1.
RefSeqiNP_001089857.1. NM_001096388.1. [Q32NN2-4]
UniGeneiXl.55586.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BL5NMR-A82-215[»]
2YMJNMR-A/B8-57[»]
DisProtiDP00286.
ProteinModelPortaliQ32NN2.
SMRiQ32NN2.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ32NN2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi734923.
KEGGixla:734923.

Organism-specific databases

CTDi734923.
XenbaseiXB-GENE-6254729. qki.

Phylogenomic databases

HOVERGENiHBG093883.
KOiK14945.

Miscellaneous databases

EvolutionaryTraceiQ32NN2.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR032367. Quaking_NLS.
IPR032377. STAR_dimer.
[Graphical view]
PfamiPF00013. KH_1. 1 hit.
PF16551. Quaking_NLS. 1 hit.
PF16544. STAR_dimer. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQKIA_XENLA
AccessioniPrimary (citable) accession number: Q32NN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: November 2, 2016
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.