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Q32NH8 (PLCD4_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4
EC=3.1.4.11
Alternative name(s):
Phosphoinositide phospholipase C-delta-4
Phospholipase C-delta-4
Short name=PLC-delta-4
Gene names
Name:plcd4
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length758 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca2+ from intracellular stores By similarity.

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactor

Binds 3 calcium ions per subunit. Two of the calcium ions are bound to the C2 domain By similarity.

Subcellular location

Membrane; Peripheral membrane protein By similarity. Nucleus By similarity. Cytoplasm By similarity. Endoplasmic reticulum By similarity. Note: Localizes primarily to intracellular membranes mostly to the endoplasmic reticulum By similarity.

Sequence similarities

Contains 1 C2 domain.

Contains 3 EF-hand domains.

Contains 1 PH domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7587581-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4
PRO_0000306830

Regions

Domain16 – 124109PH
Domain134 – 16936EF-hand 1
Domain170 – 20536EF-hand 2
Domain203 – 23735EF-hand 3
Domain290 – 435146PI-PLC X-box
Domain486 – 602117PI-PLC Y-box
Domain609 – 714106C2
Calcium binding147 – 158121 Potential
Calcium binding183 – 194122 Potential
Region26 – 5328Substrate binding By similarity
Motif726 – 7294PDZ-binding

Sites

Active site3051 By similarity
Active site3501 By similarity
Metal binding3061Calcium 1; catalytic By similarity
Metal binding3351Calcium 1; catalytic By similarity
Metal binding3371Calcium 1; catalytic By similarity
Metal binding3841Calcium 1; catalytic By similarity
Metal binding6451Calcium 2; via carbonyl oxygen By similarity
Metal binding6471Calcium 2 By similarity
Metal binding6711Calcium 2 By similarity
Metal binding7001Calcium 3 By similarity
Metal binding7011Calcium 3; via carbonyl oxygen By similarity
Metal binding7021Calcium 3 By similarity
Binding site4331Substrate By similarity
Binding site4351Substrate By similarity
Binding site5151Substrate By similarity
Binding site5421Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q32NH8 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 632DEE6D36CDF43F

FASTA75887,417
        10         20         30         40         50         60 
MTSPCSARLQ LDENLQLMQA GSPMRKVKSR SWKKQRYFKL QEDCMTIWYN SKKTGNTKST 

        70         80         90        100        110        120 
FSISDIETVR EGHQSEVLQS IAEEFKPELC FTIVFHGRRA NLDLVANTPE EAQCWIQGLE 

       130        140        150        160        170        180 
KLIETVTNMD RKDLMDQWIC DWFQKADKNK DGRMNFKEVQ DLLKMMNVDM SEHHAFRLFQ 

       190        200        210        220        230        240 
MADKSESGTL EGEEFVLFYK ALTQRDEVLK IFQDFSKDGK KLTLLEFVDF LQQGQLEEEN 

       250        260        270        280        290        300 
TEEIAMDLIA RYEPSDTAKK LHAMSIDGFL VYLCSPEGSI FNVAHEQLYQ DMMQPLCHYF 

       310        320        330        340        350        360 
ISSSHNTYLM EDQIRGQSSI EGYIRALKRG CRCVEVDTWD GPNGEPIVYH GRTFTSKILF 

       370        380        390        400        410        420 
KDVISAIDKY AFRVSDYPVI LSLENHCGVE QQDAMAQHLK SILGNKLVMS TLDGRIPVRL 

       430        440        450        460        470        480 
PSPDELRGKI LLKGKKIGRL EDSLEEQPDD SLGEVSDEEE NIEVEEERNE DKKRAKKSKE 

       490        500        510        520        530        540 
RLSQELSDCV IYCKSVPFVS FQHSRAHYIL YEMSSVTEYK ARKLVREPGN DFVRHNAWQL 

       550        560        570        580        590        600 
MRIYPTGLRT DSSNYNPQDM WNVGCQMTAL NFQTAGVEMD LNDGLFRQNA RCGYVLKPSF 

       610        620        630        640        650        660 
MRHVETTFNP DQPQGTEGYS PVNLSILVIS AQQLPKVENS KEGSIVDPLV RVEIFGVPID 

       670        680        690        700        710        720 
QTKQETKYIE NNGFNPMWYE TLHFKIHVPE LALVRFVVED YDKTSRNDFV GQYTLPFKSI 

       730        740        750 
KSGYRHIHLL SRDGTKIPPA SLFVHIRVTD ASQPEQDT

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References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC108618 mRNA. Translation: AAI08619.1.
RefSeqNP_001090146.1. NM_001096677.1.
UniGeneXl.55429.

3D structure databases

ProteinModelPortalQ32NH8.
SMRQ32NH8. Positions 194-750.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID735225.
KEGGxla:735225.

Organism-specific databases

CTD84812.
XenbaseXB-GENE-965139. plcd4.

Phylogenomic databases

HOVERGENHBG053610.
KOK05857.

Family and domain databases

Gene3D1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_Ca-dep.
IPR008973. C2_Ca/lipid-bd_dom_CaLB.
IPR018029. C2_membr_targeting.
IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. Pinositol_PLipase_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR001849. Pleckstrin_homology.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PfamPF00168. C2. 1 hit.
PF13405. EF_hand_4. 1 hit.
PF09279. efhand_like. 1 hit.
PF00169. PH. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00054. EFh. 3 hits.
SM00233. PH. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. C2_CaLB. 1 hit.
SSF51695. PLC-like_Pdiesterase_TIM-brl. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePLCD4_XENLA
AccessionPrimary (citable) accession number: Q32NH8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: December 6, 2005
Last modified: May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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