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Q32NH8

- PLCD4_XENLA

UniProt

Q32NH8 - PLCD4_XENLA

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4

Gene

plcd4

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca2+ from intracellular stores By similarity.By similarity

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Binds 3 calcium ions per subunit. Two of the calcium ions are bound to the C2 domain By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei305 – 3051PROSITE-ProRule annotation
    Metal bindingi306 – 3061Calcium 1; catalyticBy similarity
    Metal bindingi335 – 3351Calcium 1; catalyticBy similarity
    Metal bindingi337 – 3371Calcium 1; catalyticBy similarity
    Active sitei350 – 3501PROSITE-ProRule annotation
    Metal bindingi384 – 3841Calcium 1; catalyticBy similarity
    Binding sitei433 – 4331SubstrateBy similarity
    Binding sitei435 – 4351SubstrateBy similarity
    Binding sitei515 – 5151SubstrateBy similarity
    Binding sitei542 – 5421SubstrateBy similarity
    Metal bindingi645 – 6451Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi647 – 6471Calcium 2By similarity
    Metal bindingi671 – 6711Calcium 2By similarity
    Metal bindingi700 – 7001Calcium 3By similarity
    Metal bindingi701 – 7011Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi702 – 7021Calcium 3By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi147 – 158121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi183 – 194122PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. acrosome reaction Source: InterPro
    2. intracellular signal transduction Source: InterPro
    3. lipid catabolic process Source: UniProtKB-KW
    4. phosphatidylinositol metabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C-delta-4
    Phospholipase C-delta-4
    Short name:
    PLC-delta-4
    Gene namesi
    Name:plcd4
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-965139. plcd4.

    Subcellular locationi

    Membrane By similarity; Peripheral membrane protein By similarity. Nucleus By similarity. Cytoplasm By similarity. Endoplasmic reticulum By similarity
    Note: Localizes primarily to intracellular membranes mostly to the endoplasmic reticulum.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7587581-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4PRO_0000306830Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ32NH8.
    SMRiQ32NH8. Positions 194-750.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 124109PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini134 – 16936EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini170 – 20536EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini203 – 23735EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini290 – 435146PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini486 – 602117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini609 – 714106C2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni26 – 5328Substrate bindingBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi726 – 7294PDZ-binding

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 3 EF-hand domains.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOVERGENiHBG053610.
    KOiK05857.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR028387. PLC-delta4.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF31. PTHR10336:SF31. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00169. PH. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00054. EFh. 3 hits.
    SM00233. PH. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 3 hits.
    PS50003. PH_DOMAIN. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q32NH8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSPCSARLQ LDENLQLMQA GSPMRKVKSR SWKKQRYFKL QEDCMTIWYN    50
    SKKTGNTKST FSISDIETVR EGHQSEVLQS IAEEFKPELC FTIVFHGRRA 100
    NLDLVANTPE EAQCWIQGLE KLIETVTNMD RKDLMDQWIC DWFQKADKNK 150
    DGRMNFKEVQ DLLKMMNVDM SEHHAFRLFQ MADKSESGTL EGEEFVLFYK 200
    ALTQRDEVLK IFQDFSKDGK KLTLLEFVDF LQQGQLEEEN TEEIAMDLIA 250
    RYEPSDTAKK LHAMSIDGFL VYLCSPEGSI FNVAHEQLYQ DMMQPLCHYF 300
    ISSSHNTYLM EDQIRGQSSI EGYIRALKRG CRCVEVDTWD GPNGEPIVYH 350
    GRTFTSKILF KDVISAIDKY AFRVSDYPVI LSLENHCGVE QQDAMAQHLK 400
    SILGNKLVMS TLDGRIPVRL PSPDELRGKI LLKGKKIGRL EDSLEEQPDD 450
    SLGEVSDEEE NIEVEEERNE DKKRAKKSKE RLSQELSDCV IYCKSVPFVS 500
    FQHSRAHYIL YEMSSVTEYK ARKLVREPGN DFVRHNAWQL MRIYPTGLRT 550
    DSSNYNPQDM WNVGCQMTAL NFQTAGVEMD LNDGLFRQNA RCGYVLKPSF 600
    MRHVETTFNP DQPQGTEGYS PVNLSILVIS AQQLPKVENS KEGSIVDPLV 650
    RVEIFGVPID QTKQETKYIE NNGFNPMWYE TLHFKIHVPE LALVRFVVED 700
    YDKTSRNDFV GQYTLPFKSI KSGYRHIHLL SRDGTKIPPA SLFVHIRVTD 750
    ASQPEQDT 758
    Length:758
    Mass (Da):87,417
    Last modified:December 6, 2005 - v1
    Checksum:i632DEE6D36CDF43F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC108618 mRNA. Translation: AAI08619.1.
    RefSeqiNP_001090146.1. NM_001096677.1.
    UniGeneiXl.55429.

    Genome annotation databases

    GeneIDi735225.
    KEGGixla:735225.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC108618 mRNA. Translation: AAI08619.1 .
    RefSeqi NP_001090146.1. NM_001096677.1.
    UniGenei Xl.55429.

    3D structure databases

    ProteinModelPortali Q32NH8.
    SMRi Q32NH8. Positions 194-750.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 735225.
    KEGGi xla:735225.

    Organism-specific databases

    CTDi 84812.
    Xenbasei XB-GENE-965139. plcd4.

    Phylogenomic databases

    HOVERGENi HBG053610.
    KOi K05857.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR028387. PLC-delta4.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF31. PTHR10336:SF31. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00169. PH. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00054. EFh. 3 hits.
    SM00233. PH. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 3 hits.
    PS50003. PH_DOMAIN. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiPLCD4_XENLA
    AccessioniPrimary (citable) accession number: Q32NH8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3