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Protein

Myosin light chain kinase 3

Gene

MYLK3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility (By similarity).By similarity

Catalytic activityi

ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei544ATPPROSITE-ProRule annotation1
Active sitei636Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi521 – 529ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ32MK0

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain kinase 3 (EC:2.7.11.18)
Alternative name(s):
Cardiac-MyBP-C-associated Ca/CaM kinase
Short name:
Cardiac-MLCK
Gene namesi
Name:MYLK3
Synonyms:MLCK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000140795.12
HGNCiHGNC:29826 MYLK3
MIMi612147 gene
neXtProtiNX_Q32MK0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi91807
OpenTargetsiENSG00000140795
PharmGKBiPA162396375

Chemistry databases

ChEMBLiCHEMBL4627
GuidetoPHARMACOLOGYi2110

Polymorphism and mutation databases

BioMutaiMYLK3
DMDMi254763411

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002722001 – 819Myosin light chain kinase 3Add BLAST819

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei152PhosphoserineBy similarity1
Modified residuei355PhosphoserineBy similarity1
Modified residuei401PhosphoserineCombined sources1
Modified residuei408PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on serine residues.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ32MK0
PaxDbiQ32MK0
PeptideAtlasiQ32MK0
PRIDEiQ32MK0
ProteomicsDBi61603

PTM databases

iPTMnetiQ32MK0
PhosphoSitePlusiQ32MK0

Expressioni

Tissue specificityi

Restricted to heart.1 Publication

Gene expression databases

BgeeiENSG00000140795
CleanExiHS_MYLK3
GenevisibleiQ32MK0 HS

Organism-specific databases

HPAiHPA073447

Interactioni

Protein-protein interaction databases

BioGridi124881, 7 interactors
IntActiQ32MK0, 2 interactors
STRINGi9606.ENSP00000378288

Chemistry databases

BindingDBiQ32MK0

Structurei

3D structure databases

ProteinModelPortaliQ32MK0
SMRiQ32MK0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini515 – 770Protein kinasePROSITE-ProRule annotationAdd BLAST256

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0032 Eukaryota
ENOG410XRMJ LUCA
GeneTreeiENSGT00760000118877
HOGENOMiHOG000233016
HOVERGENiHBG080416
InParanoidiQ32MK0
KOiK00907
OMAiCLTTMDK
OrthoDBiEOG091G005W
PhylomeDBiQ32MK0
TreeFamiTF314166

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q32MK0-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGTSKESLG HGGLPGLGKT CLTTMDTKLN MLNEKVDQLL HFQEDVTEKL
60 70 80 90 100
QSMCRDMGHL ERGLHRLEAS RAPGPGGADG VPHIDTQAGW PEVLELVRAM
110 120 130 140 150
QQDAAQHGAR LEALFRMVAA VDRAIALVGA TFQKSKVADF LMQGRVPWRR
160 170 180 190 200
GSPGDSPEEN KERVEEEGGK PKHVLSTSGV QSDAREPGEE SQKADVLEGT
210 220 230 240 250
AERLPPIRAS GLGADPAQAV VSPGQGDGVP GPAQAFPGHL PLPTKVEAKA
260 270 280 290 300
PETPSENLRT GLELAPAPGR VNVVSPSLEV APGAGQGASS SRPDPEPLEE
310 320 330 340 350
GTRLTPGPGP QCPGPPGLPA QARATHSGGE TPPRISIHIQ EMDTPGEMLM
360 370 380 390 400
TGRGSLGPTL TTEAPAAAQP GKQGPPGTGR CLQAPGTEPG EQTPEGAREL
410 420 430 440 450
SPLQESSSPG GVKAEEEQRA GAEPGTRPSL ARSDDNDHEV GALGLQQGKS
460 470 480 490 500
PGAGNPEPEQ DCAARAPVRA EAVRRMPPGA EAGSVVLDDS PAPPAPFEHR
510 520 530 540 550
VVSVKETSIS AGYEVCQHEV LGGGRFGQVH RCTEKSTGLP LAAKIIKVKS
560 570 580 590 600
AKDREDVKNE INIMNQLSHV NLIQLYDAFE SKHSCTLVME YVDGGELFDR
610 620 630 640 650
ITDEKYHLTE LDVVLFTRQI CEGVHYLHQH YILHLDLKPE NILCVNQTGH
660 670 680 690 700
QIKIIDFGLA RRYKPREKLK VNFGTPEFLA PEVVNYEFVS FPTDMWSVGV
710 720 730 740 750
ITYMLLSGLS PFLGETDAET MNFIVNCSWD FDADTFEGLS EEAKDFVSRL
760 770 780 790 800
LVKEKSCRMS ATQCLKHEWL NNLPAKASRS KTRLKSQLLL QKYIAQRKWK
810
KHFYVVTAAN RLRKFPTSP
Length:819
Mass (Da):88,393
Last modified:July 28, 2009 - v3
Checksum:i21148BBACB0FF0D9
GO
Isoform 2 (identifier: Q32MK0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-341: Missing.

Note: No experimental confirmation available.
Show »
Length:478
Mass (Da):52,723
Checksum:i4AD65710D538D28B
GO

Sequence cautioni

The sequence AAI09098 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAC42766 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti149R → G in BAG70269 (PubMed:14702039).Curated1
Sequence conflicti199G → R in CAC42766 (Ref. 1) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05833570S → T. Corresponds to variant dbSNP:rs9923813Ensembl.1
Natural variantiVAR_058336180V → L2 PublicationsCorresponds to variant dbSNP:rs28407821Ensembl.1
Natural variantiVAR_035630390G → R in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs141602742Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0443121 – 341Missing in isoform 2. 1 PublicationAdd BLAST341

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ247087 mRNA Translation: CAC42766.1 Different initiation.
AK299443 mRNA Translation: BAH13034.1
AB451455 mRNA Translation: BAG70269.1
AC007225 Genomic DNA No translation available.
BC109097 mRNA Translation: AAI09098.2 Different initiation.
CCDSiCCDS10723.2 [Q32MK0-3]
CCDS76861.1 [Q32MK0-4]
RefSeqiNP_001295230.1, NM_001308301.1 [Q32MK0-4]
NP_872299.2, NM_182493.2 [Q32MK0-3]
XP_006721397.1, XM_006721334.3 [Q32MK0-4]
UniGeneiHs.130465

Genome annotation databases

EnsembliENST00000394809; ENSP00000378288; ENSG00000140795 [Q32MK0-3]
ENST00000536476; ENSP00000439297; ENSG00000140795 [Q32MK0-4]
GeneIDi91807
KEGGihsa:91807
UCSCiuc002eei.5 human [Q32MK0-3]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiMYLK3_HUMAN
AccessioniPrimary (citable) accession number: Q32MK0
Secondary accession number(s): B5BUL9
, B7Z5U8, Q32MK1, Q96DV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 28, 2009
Last modified: June 20, 2018
This is version 107 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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