Q32M45 (ANO4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anoctamin-4

Alternative name(s):
Transmembrane protein 16D

Gene names

Name:	ANO4
Synonyms:	TMEM16D

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	955 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Does not exhibit calcium-activated chloride channel (CaCC) activity By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein. Note: Shows an intracellular localization By similarity. Ref.7
Miscellaneous	The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.
Sequence similarities	Belongs to the anoctamin family.
Sequence caution	The sequence BAC03688.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intracellular Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q32M45-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q32M45-2)

The sequence of this isoform differs from the canonical sequence as follows:
     19-54: EGGVDLQGYQLDMQILPDGPKSDVDFSEILNAIQEM → V

Isoform 3 (identifier: Q32M45-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-433: Missing.
     466-512: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 955	955	Anoctamin-4		PRO_0000288650
Regions
	Topological domain	1 – 352	352	Extracellular Potential
	Transmembrane	353 – 373	21	Helical; Potential
	Topological domain	374 – 424	51	Cytoplasmic Potential
	Transmembrane	425 – 445	21	Helical; Potential
	Topological domain	446 – 505	60	Extracellular Potential
	Transmembrane	506 – 526	21	Helical; Potential
	Topological domain	527 – 547	21	Cytoplasmic Potential
	Transmembrane	548 – 568	21	Helical; Potential
	Topological domain	569 – 595	27	Extracellular Potential
	Transmembrane	596 – 616	21	Helical; Potential
	Topological domain	617 – 715	99	Cytoplasmic Potential
	Transmembrane	716 – 736	21	Helical; Potential
	Topological domain	737 – 768	32	Extracellular Potential
	Transmembrane	769 – 789	21	Helical; Potential
	Topological domain	790 – 885	96	Cytoplasmic Potential
	Transmembrane	886 – 906	21	Helical; Potential
	Topological domain	907 – 955	49	Extracellular Potential
	Compositional bias	462 – 467	6	Poly-Glu
Amino acid modifications
	Glycosylation	83	1	N-linked (GlcNAc...) Potential
	Glycosylation	105	1	N-linked (GlcNAc...) Potential
	Glycosylation	257	1	N-linked (GlcNAc...) Potential
	Glycosylation	288	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 433	433	Missing in isoform 3.		VSP_025741
	Alternative sequence	19 – 54	36	EGGVD…AIQEM → V in isoform 2.		VSP_025742
	Alternative sequence	466 – 512	47	Missing in isoform 3.		VSP_025743
	Natural variant	115	1	G → A. Corresponds to variant rs34162417 [ dbSNP | Ensembl ].		VAR_032453
Experimental info
	Sequence conflict	209	1	F → L in BAC03704. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 9A9348C61A4F20AF	FASTA	955	111,462
	10 20 30 40 50 60 MEASSSGITN GKTKVFHPEG GVDLQGYQLD MQILPDGPKS DVDFSEILNA IQEMAKDVNI 70 80 90 100 110 120 LFDELEAVSS PCKDDDSLLH PGNLTSTSDD ASRLEAGGET VPERNKSNGL YFRDGKCRID 130 140 150 160 170 180 YILVYRKSNP QTEKREVFER NIRAEGLQME KESSLINSDI IFVKLHAPWE VLGRYAEQMN 190 200 210 220 230 240 VRMPFRRKIY YLPRRYKFMS RIDKQISRFR RWLPKKPMRL DKETLPDLEE NDCYTAPFSQ 250 260 270 280 290 300 QRIHHFIIHN KETFFNNATR SRIVHHILQR IKYEEGKNKI GLNRLLTNGS YEAAFPLHEG 310 320 330 340 350 360 SYRSKNSIRT HGAENHRHLL YECWASWGVW YKYQPLDLVR RYFGEKIGLY FAWLGWYTGM 370 380 390 400 410 420 LFPAAFIGLF VFLYGVTTLD HSQVSKEVCQ ATDIIMCPVC DKYCPFMRLS DSCVYAKVTH 430 440 450 460 470 480 LFDNGATVFF AVFMAVWATV FLEFWKRRRA VIAYDWDLID WEEEEEEIRP QFEAKYSKKE 490 500 510 520 530 540 RMNPISGKPE PYQAFTDKCS RLIVSASGIF FMICVVIAAV FGIVIYRVVT VSTFAAFKWA 550 560 570 580 590 600 LIRNNSQVAT TGTAVCINFC IIMLLNVLYE KVALLLTNLE QPRTESEWEN SFTLKMFLFQ 610 620 630 640 650 660 FVNLNSSTFY IAFFLGRFTG HPGAYLRLIN RWRLEECHPS GCLIDLCMQM GIIMVLKQTW 670 680 690 700 710 720 NNFMELGYPL IQNWWTRRKV RQEHGPERKI SFPQWEKDYN LQPMNAYGLF DEYLEMILQF 730 740 750 760 770 780 GFTTIFVAAF PLAPLLALLN NIIEIRLDAY KFVTQWRRPL ASRAKDIGIW YGILEGIGIL 790 800 810 820 830 840 SVITNAFVIA ITSDFIPRLV YAYKYGPCAG QGEAGQKCMV GYVNASLSVF RISDFENRSE 850 860 870 880 890 900 PESDGSEFSG TPLKYCRYRD YRDPPHSLVP YGYTLQFWHV LAARLAFIIV FEHLVFCIKH 910 920 930 940 950 LISYLIPDLP KDLRDRMRRE KYLIQEMMYE AELERLQKER KERKKNGKAH HNEWP
	Isoform 2 [UniParc]. Checksum: BAA2D80DE848F4F9	FASTA	920	107,612
	10 20 30 40 50 60 MEASSSGITN GKTKVFHPVA KDVNILFDEL EAVSSPCKDD DSLLHPGNLT STSDDASRLE 70 80 90 100 110 120 AGGETVPERN KSNGLYFRDG KCRIDYILVY RKSNPQTEKR EVFERNIRAE GLQMEKESSL 130 140 150 160 170 180 INSDIIFVKL HAPWEVLGRY AEQMNVRMPF RRKIYYLPRR YKFMSRIDKQ ISRFRRWLPK 190 200 210 220 230 240 KPMRLDKETL PDLEENDCYT APFSQQRIHH FIIHNKETFF NNATRSRIVH HILQRIKYEE 250 260 270 280 290 300 GKNKIGLNRL LTNGSYEAAF PLHEGSYRSK NSIRTHGAEN HRHLLYECWA SWGVWYKYQP 310 320 330 340 350 360 LDLVRRYFGE KIGLYFAWLG WYTGMLFPAA FIGLFVFLYG VTTLDHSQVS KEVCQATDII 370 380 390 400 410 420 MCPVCDKYCP FMRLSDSCVY AKVTHLFDNG ATVFFAVFMA VWATVFLEFW KRRRAVIAYD 430 440 450 460 470 480 WDLIDWEEEE EEIRPQFEAK YSKKERMNPI SGKPEPYQAF TDKCSRLIVS ASGIFFMICV 490 500 510 520 530 540 VIAAVFGIVI YRVVTVSTFA AFKWALIRNN SQVATTGTAV CINFCIIMLL NVLYEKVALL 550 560 570 580 590 600 LTNLEQPRTE SEWENSFTLK MFLFQFVNLN SSTFYIAFFL GRFTGHPGAY LRLINRWRLE 610 620 630 640 650 660 ECHPSGCLID LCMQMGIIMV LKQTWNNFME LGYPLIQNWW TRRKVRQEHG PERKISFPQW 670 680 690 700 710 720 EKDYNLQPMN AYGLFDEYLE MILQFGFTTI FVAAFPLAPL LALLNNIIEI RLDAYKFVTQ 730 740 750 760 770 780 WRRPLASRAK DIGIWYGILE GIGILSVITN AFVIAITSDF IPRLVYAYKY GPCAGQGEAG 790 800 810 820 830 840 QKCMVGYVNA SLSVFRISDF ENRSEPESDG SEFSGTPLKY CRYRDYRDPP HSLVPYGYTL 850 860 870 880 890 900 QFWHVLAARL AFIIVFEHLV FCIKHLISYL IPDLPKDLRD RMRREKYLIQ EMMYEAELER 910 920 LQKERKERKK NGKAHHNEWP
	Isoform 3 [UniParc]. Checksum: E6FC96E22BA79519	FASTA	475	55,719
	10 20 30 40 50 60 MAVWATVFLE FWKRRRAVIA YDWDLIDWEE EEICVVIAAV FGIVIYRVVT VSTFAAFKWA 70 80 90 100 110 120 LIRNNSQVAT TGTAVCINFC IIMLLNVLYE KVALLLTNLE QPRTESEWEN SFTLKMFLFQ 130 140 150 160 170 180 FVNLNSSTFY IAFFLGRFTG HPGAYLRLIN RWRLEECHPS GCLIDLCMQM GIIMVLKQTW 190 200 210 220 230 240 NNFMELGYPL IQNWWTRRKV RQEHGPERKI SFPQWEKDYN LQPMNAYGLF DEYLEMILQF 250 260 270 280 290 300 GFTTIFVAAF PLAPLLALLN NIIEIRLDAY KFVTQWRRPL ASRAKDIGIW YGILEGIGIL 310 320 330 340 350 360 SVITNAFVIA ITSDFIPRLV YAYKYGPCAG QGEAGQKCMV GYVNASLSVF RISDFENRSE 370 380 390 400 410 420 PESDGSEFSG TPLKYCRYRD YRDPPHSLVP YGYTLQFWHV LAARLAFIIV FEHLVFCIKH 430 440 450 460 470 LISYLIPDLP KDLRDRMRRE KYLIQEMMYE AELERLQKER KERKKNGKAH HNEWP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Brain and Prostate.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"FLJ10261 gene, located within the CCND1-EMS1 locus on human chromosome 11q13, encodes the eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products." Katoh M., Katoh M. Int. J. Oncol. 22:1375-1381(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[4]	"Physiological roles and diseases of Tmem16/Anoctamin proteins: are they all chloride channels?" Duran C., Hartzell H.C. Acta Pharmacol. Sin. 32:685-692(2011) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[5]	"Anoctamins." Kunzelmann K., Tian Y., Martins J.R., Faria D., Kongsuphol P., Ousingsawat J., Thevenod F., Roussa E., Rock J., Schreiber R. Pflugers Arch. 462:195-208(2011) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[6]	"The anoctamin (TMEM16) gene family: calcium-activated chloride channels come of age." Winpenny J.P., Gray M.A. Exp. Physiol. 97:175-176(2012) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[7]	"Anoctamins are a family of Ca2+ activated Cl- channels." Tian Y., Schreiber R., Kunzelmann K. J. Cell Sci. 125:4991-4998(2012) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK091540 mRNA. Translation: BAC03688.1. Different initiation. AK091591 mRNA. Translation: BAC03704.1. AK092596 mRNA. Translation: BAC03924.1. BC109308 mRNA. Translation: AAI09309.1.
IPI	IPI00177878. IPI00249140. IPI00847500.
RefSeq	NP_849148.2. NM_178826.3.
UniGene	Hs.58785.
3D structure databases
ProteinModelPortal	Q32M45.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000376705.
PTM databases
PhosphoSite	Q32M45.
Polymorphism databases
DMDM	121942141.
Proteomic databases
PaxDb	Q32M45.
PRIDE	Q32M45.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000299222; ENSP00000299222; ENSG00000151572. ENST00000392977; ENSP00000376703; ENSG00000151572. ENST00000392979; ENSP00000376705; ENSG00000151572. ENST00000550015; ENSP00000450192; ENSG00000151572. ENST00000570509; ENSP00000471431; ENSG00000262139. ENST00000573650; ENSP00000469449; ENSG00000262139. ENST00000575847; ENSP00000470039; ENSG00000262139. ENST00000594618; ENSP00000471210; ENSG00000262139.
GeneID	121601.
KEGG	hsa:121601.
UCSC	uc001thw.2. human. uc001thx.2. human. uc001thy.2. human.
Organism-specific databases
CTD	121601.
GeneCards	GC12P101111.
HGNC	HGNC:23837. ANO4.
HPA	HPA053412.
MIM	610111. gene.
neXtProt	NX_Q32M45.
PharmGKB	PA164715582.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG320103.
HOGENOM	HOG000006509.
HOVERGEN	HBG069519.
OMA	TEYEDSY.
Gene expression databases
ArrayExpress	Q32M45.
Bgee	Q32M45.
CleanEx	HS_ANO4.
Genevestigator	Q32M45.
Family and domain databases
InterPro	IPR007632. Anoctamin. [Graphical view]
PANTHER	PTHR12308. PTHR12308. 1 hit.
Pfam	PF04547. Anoctamin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ANO4. human.
GenomeRNAi	121601.
NextBio	80786.
SOURCE	Search...

Entry information

Entry name

ANO4_HUMAN

Accession

Primary (citable) accession number: Q32M45
Secondary accession number(s): Q8NAJ0, Q8NB39, Q8NB53

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	December 6, 2005
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.