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Protein

tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like

Gene

Dus4l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.By similarity

Cofactori

FMNBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87FMNBy similarity1
Active sitei116Proton donorBy similarity1
Binding sitei158FMNBy similarity1
Binding sitei186FMNBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi33 – 35FMNBy similarity3
Nucleotide bindingi216 – 218FMNBy similarity3
Nucleotide bindingi240 – 241FMNBy similarity2

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Flavoprotein, FMN

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like (EC:1.3.1.-)
Alternative name(s):
tRNA-dihydrouridine synthase 4-like
Gene namesi
Name:Dus4l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1919166. Dus4l.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002473481 – 324tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-likeAdd BLAST324

Proteomic databases

EPDiQ32M08.
MaxQBiQ32M08.
PaxDbiQ32M08.
PRIDEiQ32M08.

PTM databases

PhosphoSitePlusiQ32M08.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020648.
CleanExiMM_DUS4L.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020977.

Structurei

3D structure databases

ProteinModelPortaliQ32M08.
SMRiQ32M08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Dus family. Dus4 subfamily.Curated

Phylogenomic databases

eggNOGiKOG2335. Eukaryota.
COG0042. LUCA.
HOGENOMiHOG000203649.
HOVERGENiHBG053313.
InParanoidiQ32M08.
KOiK05545.
PhylomeDBiQ32M08.
TreeFamiTF105618.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 1 hit.
PfamiPF01207. Dus. 1 hit.
[Graphical view]
PIRSFiPIRSF006621. Dus. 1 hit.
PROSITEiPS01136. UPF0034. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q32M08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSDSLPTTI CQERKKDPIE MFHSGQLVKV CAPMVRYSKL AFRTLVRKYS
60 70 80 90 100
CDLCYTPMII AADFVRSIKA RDSEFTTNQG DCPLIVQFAA NDARLLSDAA
110 120 130 140 150
LLVCPYANGI DINCGCPQRW AMADGYGACL INKPELVHDM VRQVRNRVES
160 170 180 190 200
PRFSVSIKIR IHDDLARTID LCRKAEATGV SWITVHGRTV EERHQPVHYD
210 220 230 240 250
AIKMIKENVS IPIVANGDIR SLKEAENVWQ MTGTDGVMVA RGLLANPAMF
260 270 280 290 300
AGYEETPLKC IWDWVDISLE LGTPFMCFHQ HLMYMMEKIT SRQEKRVFNA
310 320
LSSTSAVLDY LTDHYGDESL SKSL
Length:324
Mass (Da):36,620
Last modified:December 6, 2005 - v1
Checksum:i0E6FB06B8F1A3B7D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti245A → T in BAB26724 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010138 mRNA. Translation: BAB26724.1.
BC109351 mRNA. Translation: AAI09352.1.
CCDSiCCDS25865.1.
RefSeqiNP_082278.1. NM_028002.2.
UniGeneiMm.62979.

Genome annotation databases

GeneIDi71916.
KEGGimmu:71916.
UCSCiuc007nhr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010138 mRNA. Translation: BAB26724.1.
BC109351 mRNA. Translation: AAI09352.1.
CCDSiCCDS25865.1.
RefSeqiNP_082278.1. NM_028002.2.
UniGeneiMm.62979.

3D structure databases

ProteinModelPortaliQ32M08.
SMRiQ32M08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020977.

PTM databases

PhosphoSitePlusiQ32M08.

Proteomic databases

EPDiQ32M08.
MaxQBiQ32M08.
PaxDbiQ32M08.
PRIDEiQ32M08.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi71916.
KEGGimmu:71916.
UCSCiuc007nhr.1. mouse.

Organism-specific databases

CTDi11062.
MGIiMGI:1919166. Dus4l.

Phylogenomic databases

eggNOGiKOG2335. Eukaryota.
COG0042. LUCA.
HOGENOMiHOG000203649.
HOVERGENiHBG053313.
InParanoidiQ32M08.
KOiK05545.
PhylomeDBiQ32M08.
TreeFamiTF105618.

Miscellaneous databases

PROiQ32M08.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020648.
CleanExiMM_DUS4L.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 1 hit.
PfamiPF01207. Dus. 1 hit.
[Graphical view]
PIRSFiPIRSF006621. Dus. 1 hit.
PROSITEiPS01136. UPF0034. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDUS4L_MOUSE
AccessioniPrimary (citable) accession number: Q32M08
Secondary accession number(s): Q9D6P4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.