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Protein

Adenylate kinase 8

Gene

Ak8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP. Also displays broad nucleoside diphosphate kinase activity.By similarity

Catalytic activityi

ATP + AMP = 2 ADP.By similarity
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei147 – 1471AMP 1By similarity
Binding sitei203 – 2031AMP 1By similarity
Binding sitei361 – 3611AMP 2By similarity
Binding sitei392 – 3921ATP 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi67 – 726ATP 1By similarity
Nucleotide bindingi140 – 1434AMP 1By similarity
Nucleotide bindingi278 – 2836ATP 2By similarity
Nucleotide bindingi325 – 3273AMP 2By similarity
Nucleotide bindingi354 – 3574AMP 2By similarity

GO - Molecular functioni

GO - Biological processi

  • nucleoside diphosphate phosphorylation Source: UniProtKB
  • nucleoside triphosphate biosynthetic process Source: UniProtKB
  • ventricular system development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase 8 (EC:2.7.4.3, EC:2.7.4.6)
Short name:
AK 8
Alternative name(s):
ATP-AMP transphosphorylase 8
Gene namesi
Name:Ak8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1916120. Ak8.

Subcellular locationi

  • Cytoplasmcytosol By similarity

GO - Cellular componenti

  • axoneme Source: MGI
  • cytosol Source: UniProtKB-SubCell
  • sperm flagellum Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479Adenylate kinase 8PRO_0000279385Add
BLAST

Proteomic databases

MaxQBiQ32M07.
PaxDbiQ32M07.
PRIDEiQ32M07.

PTM databases

PhosphoSiteiQ32M07.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026807.
CleanExiMM_1190002A17RIK.
GenevisibleiQ32M07. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000073789.

Structurei

3D structure databases

ProteinModelPortaliQ32M07.
SMRiQ32M07. Positions 59-250, 270-477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni58 – 258201Adenylate kinase 1By similarityAdd
BLAST
Regioni87 – 11327NMPbind 1By similarityAdd
BLAST
Regioni177 – 20630LID 1By similarityAdd
BLAST
Regioni269 – 471203Adenylate kinase 2By similarityAdd
BLAST
Regioni298 – 32730NMPbind 2By similarityAdd
BLAST
Regioni391 – 42434LID 2By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the adenylate kinase family.Curated

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
KOG3079. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00830000128340.
HOVERGENiHBG080569.
InParanoidiQ32M07.
KOiK00939.
OMAiMQNMLEQ.
OrthoDBiEOG091G06BH.
PhylomeDBiQ32M07.
TreeFamiTF328560.

Family and domain databases

CDDicd01428. ADK. 2 hits.
Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 3 hits.
SSF57774. SSF57774. 2 hits.

Sequencei

Sequence statusi: Complete.

Q32M07-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDATTAPHRI PPEMPQYGED YYIFEMMQNM LEQLLIHQPE DPISFMITHL
60 70 80 90 100
RRNNDNVPKV VILGPPASGK TTIAMWLCKH LNSNLITKES LLEREFSRLS
110 120 130 140 150
VEAKSYYQVY KKIPNSILVS LVQERLNEDD CLRKGWILDG IPERREQALM
160 170 180 190 200
IQTLGLAPKH VIVLNAPDTV LIERNVGKRI DPVTGEIYHT TFDWPPEPEI
210 220 230 240 250
QNRLRQPEGI SEIETAKKLL EYHRHIIRIL PSYPKILKTI SSDQPCVDVF
260 270 280 290 300
YQALTYVQSG HRCNAPFTPK VLLCGPLGSG KRLQATLLAQ KYGLVNISCG
310 320 330 340 350
QLLKEAVAAK SSFGELIQPF FEKRMTVPDS IITKVLADRM EQQDCIQKGW
360 370 380 390 400
VLHGFPRDLD QARMLNSMGY NPNRVFFLSV PLDSILERLT LRRTDPVTGE
410 420 430 440 450
RFHLMYKPPP TIEVQVRLLQ NPKDSEEYIK LQTDLFYRNS GDLEQYYDRA
460 470
IIVNGDQDPY TVFEYIESGI INPLPRKVT
Length:479
Mass (Da):55,073
Last modified:December 6, 2005 - v1
Checksum:i4475836B92D069E1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti468 – 4681S → G in AAI00482 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL732526, AL731851 Genomic DNA. Translation: CAM21461.1.
AL731851, AL732526 Genomic DNA. Translation: CAM22298.1.
BC100481 mRNA. Translation: AAI00482.1.
BC109352 mRNA. Translation: AAI09353.1.
CCDSiCCDS15846.1.
RefSeqiNP_001029046.2. NM_001033874.2.
UniGeneiMm.273318.

Genome annotation databases

EnsembliENSMUST00000074156; ENSMUSP00000073789; ENSMUSG00000026807.
GeneIDi68870.
KEGGimmu:68870.
UCSCiuc008izd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL732526, AL731851 Genomic DNA. Translation: CAM21461.1.
AL731851, AL732526 Genomic DNA. Translation: CAM22298.1.
BC100481 mRNA. Translation: AAI00482.1.
BC109352 mRNA. Translation: AAI09353.1.
CCDSiCCDS15846.1.
RefSeqiNP_001029046.2. NM_001033874.2.
UniGeneiMm.273318.

3D structure databases

ProteinModelPortaliQ32M07.
SMRiQ32M07. Positions 59-250, 270-477.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000073789.

PTM databases

PhosphoSiteiQ32M07.

Proteomic databases

MaxQBiQ32M07.
PaxDbiQ32M07.
PRIDEiQ32M07.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000074156; ENSMUSP00000073789; ENSMUSG00000026807.
GeneIDi68870.
KEGGimmu:68870.
UCSCiuc008izd.1. mouse.

Organism-specific databases

CTDi158067.
MGIiMGI:1916120. Ak8.

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
KOG3079. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00830000128340.
HOVERGENiHBG080569.
InParanoidiQ32M07.
KOiK00939.
OMAiMQNMLEQ.
OrthoDBiEOG091G06BH.
PhylomeDBiQ32M07.
TreeFamiTF328560.

Enzyme and pathway databases

ReactomeiR-MMU-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

ChiTaRSiAk8. mouse.
PROiQ32M07.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026807.
CleanExiMM_1190002A17RIK.
GenevisibleiQ32M07. MM.

Family and domain databases

CDDicd01428. ADK. 2 hits.
Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 3 hits.
SSF57774. SSF57774. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiKAD8_MOUSE
AccessioniPrimary (citable) accession number: Q32M07
Secondary accession number(s): Q497L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: December 6, 2005
Last modified: September 7, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.