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Protein

Fascin-2

Gene

Fscn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as an actin bundling protein. May play a pivotal role in photoreceptor cell-specific events, such as disk morphogenesis. Important for maintaining functional hair-cell bundless in the inner ear. May stiffen the longer stereocilia of hair-cell bundles in the inner ear enabling better force transmission to tip links.1 Publication

GO - Molecular functioni

  1. actin binding Source: UniProtKB
  2. actin filament binding Source: UniProtKB

GO - Biological processi

  1. actin cytoskeleton organization Source: UniProtKB
  2. eye photoreceptor cell development Source: MGI
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Fascin-2
Alternative name(s):
Retinal fascin
Gene namesi
Name:Fscn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:2443337. Fscn2.

Subcellular locationi

Cytoplasmcytoskeleton 1 Publication. Cell projectionstereocilium 1 Publication
Note: Found mostly in longer hair-cell stereocilia and, in all stereocilia, present in gradient with the highest concentration at stereocilia tips.

GO - Cellular componenti

  1. actin cytoskeleton Source: UniProtKB
  2. cytoplasm Source: UniProtKB-KW
  3. stereocilium Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Involvement in diseasei

Defects in Fscn2 lead to hair-cell degeneration in the inner ear and are a key contributor to the early-onset, age-related hearing loss (prebycusis) phenotype when in combination with waltzer cadherin 23 mutant.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492Fascin-2PRO_0000404202Add
BLAST

Proteomic databases

PaxDbiQ32M02.
PRIDEiQ32M02.

Expressioni

Tissue specificityi

Expressed in the inner ear.

Developmental stagei

Developmentally regulated, appearing in inner-hair cell stereocilia during final stages of elongation.

Gene expression databases

BgeeiQ32M02.
GenevestigatoriQ32M02.

Structurei

3D structure databases

ProteinModelPortaliQ32M02.
SMRiQ32M02. Positions 8-492.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the fascin family.Curated

Phylogenomic databases

eggNOGiNOG85029.
GeneTreeiENSGT00530000063373.
HOGENOMiHOG000267034.
HOVERGENiHBG000968.
InParanoidiQ32M02.
KOiK17455.
OMAiYQIRGRG.
OrthoDBiEOG7VQJCP.
PhylomeDBiQ32M02.
TreeFamiTF323992.

Family and domain databases

InterProiIPR008999. Actin_cross-linking.
IPR010431. Fascin.
IPR022768. Fascin-domain.
IPR024703. Fascin_metazoans.
IPR030144. FSCN2.
[Graphical view]
PANTHERiPTHR10551. PTHR10551. 1 hit.
PTHR10551:SF4. PTHR10551:SF4. 1 hit.
PfamiPF06268. Fascin. 4 hits.
[Graphical view]
PIRSFiPIRSF005682. Fascin. 1 hit.
SUPFAMiSSF50405. SSF50405. 4 hits.

Sequencei

Sequence statusi: Complete.

Q32M02-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPTNGLHQVL KIQFGLVNDA DRYLTAESFG FKVNASAASL KRKQIWVLEP
60 70 80 90 100
DPGQGTAVLF RSSHLGRYLS AEEDGRVACE MDQPGRDCRF LVLPQPDGRW
110 120 130 140 150
VLQSEPHGRF FGGIEDRLSC FATAISPAEL WTVHLAIHPQ AHLLSVSRRR
160 170 180 190 200
YVHLCLQEDE MAADGDMPWG VDALVTLIFQ SRRYCLKSYD SRYLRSDGRL
210 220 230 240 250
VWEPEAHACY TLEFKAGKLA FKDCDGRYLA PVGPAGTLKA GRNTRPSKDE
260 270 280 290 300
LFDLEQSHPQ VVLVAANRRY ISVRQGINVS ANQDEELGHE TFLMQIDQET
310 320 330 340 350
KKCTFYSSTG GYWTLVTHGG IQATATQVSA NTMFEIEWHG RRVALKASNG
360 370 380 390 400
RFVCMKKNGQ LAAISDFVGE DELFTLKLIN RPLLVLRGLD GFVCHRRGSN
410 420 430 440 450
QLDTNRSTYD VFHLSFRDGA YQIRGRGGGF WYTGSHGSVC SDGDLAEDFL
460 470 480 490
FEFRERGRLA IRALSGKYLR GGASGLLRAD ADLPVGEALW EY
Length:492
Mass (Da):55,073
Last modified:December 6, 2005 - v1
Checksum:i8B848783E8E778D5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti395 – 3951H → P in BAC34026. (PubMed:16141072)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti109 – 1091R → H in prebycusis phenotype; when associated with waltzer cadherin 23 mutant. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050002 mRNA. Translation: BAC34026.1.
AL669855 Genomic DNA. Translation: CAM27038.1.
CH466558 Genomic DNA. Translation: EDL34739.1.
BC109356 mRNA. Translation: AAI09357.1.
BC109357 mRNA. Translation: AAI09358.1.
CCDSiCCDS25731.1.
RefSeqiNP_766390.2. NM_172802.4.
UniGeneiMm.481403.

Genome annotation databases

EnsembliENSMUST00000026445; ENSMUSP00000026445; ENSMUSG00000025380.
GeneIDi238021.
KEGGimmu:238021.
UCSCiuc007msk.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050002 mRNA. Translation: BAC34026.1.
AL669855 Genomic DNA. Translation: CAM27038.1.
CH466558 Genomic DNA. Translation: EDL34739.1.
BC109356 mRNA. Translation: AAI09357.1.
BC109357 mRNA. Translation: AAI09358.1.
CCDSiCCDS25731.1.
RefSeqiNP_766390.2. NM_172802.4.
UniGeneiMm.481403.

3D structure databases

ProteinModelPortaliQ32M02.
SMRiQ32M02. Positions 8-492.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ32M02.
PRIDEiQ32M02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026445; ENSMUSP00000026445; ENSMUSG00000025380.
GeneIDi238021.
KEGGimmu:238021.
UCSCiuc007msk.2. mouse.

Organism-specific databases

CTDi25794.
MGIiMGI:2443337. Fscn2.

Phylogenomic databases

eggNOGiNOG85029.
GeneTreeiENSGT00530000063373.
HOGENOMiHOG000267034.
HOVERGENiHBG000968.
InParanoidiQ32M02.
KOiK17455.
OMAiYQIRGRG.
OrthoDBiEOG7VQJCP.
PhylomeDBiQ32M02.
TreeFamiTF323992.

Miscellaneous databases

NextBioi383636.
PROiQ32M02.
SOURCEiSearch...

Gene expression databases

BgeeiQ32M02.
GenevestigatoriQ32M02.

Family and domain databases

InterProiIPR008999. Actin_cross-linking.
IPR010431. Fascin.
IPR022768. Fascin-domain.
IPR024703. Fascin_metazoans.
IPR030144. FSCN2.
[Graphical view]
PANTHERiPTHR10551. PTHR10551. 1 hit.
PTHR10551:SF4. PTHR10551:SF4. 1 hit.
PfamiPF06268. Fascin. 4 hits.
[Graphical view]
PIRSFiPIRSF005682. Fascin. 1 hit.
SUPFAMiSSF50405. SSF50405. 4 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Hippocampus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The R109H variant of fascin-2, a developmentally regulated actin crosslinker in hair-cell stereocilia, underlies early-onset hearing loss of DBA/2J mice."
    Shin J.B., Longo-Guess C.M., Gagnon L.H., Saylor K.W., Dumont R.A., Spinelli K.J., Pagana J.M., Wilmarth P.A., David L.L., Gillespie P.G., Johnson K.R.
    J. Neurosci. 30:9683-9694(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, VARIANT PREBYCUSIS HIS-109.

Entry informationi

Entry nameiFSCN2_MOUSE
AccessioniPrimary (citable) accession number: Q32M02
Secondary accession number(s): Q8BWU0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: December 6, 2005
Last modified: January 7, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.