Q32LQ4 (BHMT1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Betaine--homocysteine S-methyltransferase 1
EC=2.1.1.5

Gene names

Name:	bhmt
ORF Names:	wu:fb53h01, zgc:123027

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline By similarity.
Catalytic activity	Trimethylammonioacetate + L-homocysteine = dimethylglycine + L-methionine.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 Hcy-binding domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	betaine-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Betaine--homocysteine S-methyltransferase 1		PRO_0000273221
Regions
	Domain	8 – 309	302	Hcy-binding
Sites
	Metal binding	212	1	Zinc By similarity
	Metal binding	294	1	Zinc By similarity
	Metal binding	295	1	Zinc By similarity
Experimental info
	Sequence conflict	189	1	H → P in AAV74219. Ref.1
	Sequence conflict	192	1	I → T in AAV74219. Ref.1
	Sequence conflict	224	1	A → V in AAV74219. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q32LQ4 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: A6A3A9A824D5D26A	FASTA	400	44,066
	10 20 30 40 50 60 MAPVGSKRGV LERLNAGEVV IGDGGFVFAL EKRGYVKAGP WTPEAAAEHP EAVRQLHREF 70 80 90 100 110 120 LRAGSNVMQT FTFYASDDKL ENRGNKLSFT GQQINEAACD LAREVANEGD ALVAGGVSQT 130 140 150 160 170 180 PSYLSCKSEE EVKKTFKKQL DVFIKKNVDL LIAEYFEHVE EAEWAVQVLK ATGKPVAATL 190 200 210 220 230 240 CIGPDGDMHG VIPGECAVRL VKAGADIVGV NCHFDPLTCV KTVAMMKAAV EKAGLKAHYM 250 260 270 280 290 300 TQPLAYHTPD CSCQGFIDLP EFPFALEPRI LTRWEMQQYA REAYKAGIRY IGGCCGFEPY 310 320 330 340 350 360 HIRAVAEELS AERGFLPEAS QKHGLWGSGL EMHTKPWVRA RARRDYWEKL KPASGRPLCP 370 380 390 400 SMSTPDGWGV TRGHAALMQQ KEATTAEQLR PLFQQADAKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular genetic analysis of folate metabolism in the zebrafish." Lapek J.D. Jr., Warren J.T. Jr. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY830415 mRNA. Translation: AAV74219.1. BC109472 mRNA. Translation: AAI09473.1.
IPI	IPI00491662.
RefSeq	NP_001012498.1. NM_001012480.1.
UniGene	Dr.75610.
3D structure databases
ProteinModelPortal	Q32LQ4.
SMR	Q32LQ4. Positions 7-393.
ModBase	Search...
Protein-protein interaction databases
STRING	Q32LQ4.
Proteomic databases
PRIDE	Q32LQ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	322228.
KEGG	dre:322228.
Organism-specific databases
CTD	635.
ZFIN	ZDB-GENE-030131-947. bhmt.
Phylogenomic databases
eggNOG	fiNOG08394.
GeneTree	ENSGT00390000003122.
HOGENOM	HBG713318.
HOVERGEN	HBG080367.
OrthoDB	EOG4001JM.
PhylomeDB	Q32LQ4.
Gene expression databases
ArrayExpress	Q32LQ4.
Bgee	Q32LQ4.
Family and domain databases
InterPro	IPR017226. Betaine-hCys_S-MeTrfase_BHMT. IPR003726. S_MeTrfase. [Graphical view]
Gene3D	G3DSA:3.20.20.330. S_methyl_trans. 1 hit.
KO	K00544.
Pfam	PF02574. S-methyl_trans. 1 hit. [Graphical view]
PIRSF	PIRSF037505. Betaine_HMT. 1 hit.
SUPFAM	SSF82282. S_methyl_trans. 1 hit.
PROSITE	PS50970. HCY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BHMT1_DANRE

Accession

Primary (citable) accession number: Q32LQ4
Secondary accession number(s): Q5PSM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	December 6, 2005
Last modified:	November 16, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program