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Protein

Betaine--homocysteine S-methyltransferase 1

Gene

bhmt

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline (By similarity).By similarity

Catalytic activityi

Trimethylammonioacetate + L-homocysteine = dimethylglycine + L-methionine.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: betaine degradation

This protein is involved in step 1 of the subpathway that synthesizes sarcosine from betaine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Betaine--homocysteine S-methyltransferase 1 (bhmt)
  2. no protein annotated in this organism
This subpathway is part of the pathway betaine degradation, which is itself part of Amine and polyamine degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sarcosine from betaine, the pathway betaine degradation and in Amine and polyamine degradation.

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (BhmT route).
Proteins known to be involved in this subpathway in this organism are:
  1. Betaine--homocysteine S-methyltransferase 1 (bhmt), Betaine--homocysteine S-methyltransferase 1 (bhmt)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (BhmT route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi212ZincPROSITE-ProRule annotation1
Metal bindingi294ZincPROSITE-ProRule annotation1
Metal bindingi295ZincPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • amino-acid betaine catabolic process Source: UniProtKB-UniPathway
  • digestive system development Source: ZFIN
  • methionine biosynthetic process Source: InterPro
  • type B pancreatic cell development Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00051; UER00083.
UPA00291; UER00432.

Names & Taxonomyi

Protein namesi
Recommended name:
Betaine--homocysteine S-methyltransferase 1 (EC:2.1.1.5)
Gene namesi
Name:bhmt
ORF Names:wu:fb53h01, zgc:123027
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-030131-947. bhmt.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002732211 – 400Betaine--homocysteine S-methyltransferase 1Add BLAST400

Proteomic databases

PaxDbiQ32LQ4.
PRIDEiQ32LQ4.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000040421.

Structurei

3D structure databases

ProteinModelPortaliQ32LQ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 309Hcy-bindingPROSITE-ProRule annotationAdd BLAST302

Sequence similaritiesi

Contains 1 Hcy-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1579. Eukaryota.
COG0646. LUCA.
HOGENOMiHOG000231636.
HOVERGENiHBG080367.
InParanoidiQ32LQ4.
KOiK00544.
PhylomeDBiQ32LQ4.

Family and domain databases

Gene3Di3.20.20.330. 1 hit.
InterProiIPR017226. Betaine-hCys_S-MeTrfase_BHMT.
IPR003726. HCY_dom.
[Graphical view]
PfamiPF02574. S-methyl_trans. 1 hit.
[Graphical view]
PIRSFiPIRSF037505. Betaine_HMT. 1 hit.
SUPFAMiSSF82282. SSF82282. 1 hit.
PROSITEiPS50970. HCY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q32LQ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPVGSKRGV LERLNAGEVV IGDGGFVFAL EKRGYVKAGP WTPEAAAEHP
60 70 80 90 100
EAVRQLHREF LRAGSNVMQT FTFYASDDKL ENRGNKLSFT GQQINEAACD
110 120 130 140 150
LAREVANEGD ALVAGGVSQT PSYLSCKSEE EVKKTFKKQL DVFIKKNVDL
160 170 180 190 200
LIAEYFEHVE EAEWAVQVLK ATGKPVAATL CIGPDGDMHG VIPGECAVRL
210 220 230 240 250
VKAGADIVGV NCHFDPLTCV KTVAMMKAAV EKAGLKAHYM TQPLAYHTPD
260 270 280 290 300
CSCQGFIDLP EFPFALEPRI LTRWEMQQYA REAYKAGIRY IGGCCGFEPY
310 320 330 340 350
HIRAVAEELS AERGFLPEAS QKHGLWGSGL EMHTKPWVRA RARRDYWEKL
360 370 380 390 400
KPASGRPLCP SMSTPDGWGV TRGHAALMQQ KEATTAEQLR PLFQQADAKH
Length:400
Mass (Da):44,066
Last modified:December 6, 2005 - v1
Checksum:iA6A3A9A824D5D26A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti189H → P in AAV74219 (Ref. 1) Curated1
Sequence conflicti192I → T in AAV74219 (Ref. 1) Curated1
Sequence conflicti224A → V in AAV74219 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY830415 mRNA. Translation: AAV74219.1.
BC109472 mRNA. Translation: AAI09473.1.
RefSeqiNP_001012498.1. NM_001012480.1.
UniGeneiDr.75610.

Genome annotation databases

GeneIDi322228.
KEGGidre:322228.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY830415 mRNA. Translation: AAV74219.1.
BC109472 mRNA. Translation: AAI09473.1.
RefSeqiNP_001012498.1. NM_001012480.1.
UniGeneiDr.75610.

3D structure databases

ProteinModelPortaliQ32LQ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000040421.

Proteomic databases

PaxDbiQ32LQ4.
PRIDEiQ32LQ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi322228.
KEGGidre:322228.

Organism-specific databases

CTDi635.
ZFINiZDB-GENE-030131-947. bhmt.

Phylogenomic databases

eggNOGiKOG1579. Eukaryota.
COG0646. LUCA.
HOGENOMiHOG000231636.
HOVERGENiHBG080367.
InParanoidiQ32LQ4.
KOiK00544.
PhylomeDBiQ32LQ4.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00083.
UPA00291; UER00432.

Miscellaneous databases

PROiQ32LQ4.

Family and domain databases

Gene3Di3.20.20.330. 1 hit.
InterProiIPR017226. Betaine-hCys_S-MeTrfase_BHMT.
IPR003726. HCY_dom.
[Graphical view]
PfamiPF02574. S-methyl_trans. 1 hit.
[Graphical view]
PIRSFiPIRSF037505. Betaine_HMT. 1 hit.
SUPFAMiSSF82282. SSF82282. 1 hit.
PROSITEiPS50970. HCY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBHMT1_DANRE
AccessioniPrimary (citable) accession number: Q32LQ4
Secondary accession number(s): Q5PSM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: December 6, 2005
Last modified: October 5, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.