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Protein

Cytochrome b5 reductase 4

Gene

CYB5R4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS) (By similarity).By similarity

Catalytic activityi

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi89Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi112Iron (heme axial ligand)PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi364 – 379FADBy similarityAdd BLAST16
Nucleotide bindingi391 – 423FADBy similarityAdd BLAST33

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5 reductase 4 (EC:1.6.2.2)
Alternative name(s):
Flavohemoprotein b5/b5R
Short name:
b5+b5R
cb5/cb5R
Gene namesi
Name:CYB5R4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002875551 – 520Cytochrome b5 reductase 4Add BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ32LH7.
PRIDEiQ32LH7.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000051953.

Structurei

3D structure databases

ProteinModelPortaliQ32LH7.
SMRiQ32LH7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 130Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST77
Domaini164 – 255CSPROSITE-ProRule annotationAdd BLAST92
Domaini272 – 384FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
KOG0536. Eukaryota.
COG0543. LUCA.
COG5274. LUCA.
HOGENOMiHOG000230826.
HOVERGENiHBG108174.
InParanoidiQ32LH7.
KOiK00326.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
3.10.120.10. 1 hit.
InterProiView protein in InterPro
IPR007052. CS_dom.
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR036400. Cyt_B5-like_heme/steroid_sf.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR008978. HSP20-like_chaperone.
IPR001834. NADH-Cyt_B5_reductase.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
PfamiView protein in Pfam
PF04969. CS. 1 hit.
PF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
SMARTiView protein in SMART
SM01117. Cyt-b5. 1 hit.
SUPFAMiSSF49764. SSF49764. 1 hit.
SSF55856. SSF55856. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiView protein in PROSITE
PS51203. CS. 1 hit.
PS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.

Sequencei

Sequence statusi: Complete.

Q32LH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNVPSQSFP GPSSQQRVAS GGRSKVPLKQ GRSLMDWIRL TKSGKDLTGL
60 70 80 90 100
KGRLIEVTEE ELKKHNKKDD CWICIRGFVY NVSPYMEYHP GGEDELMRAA
110 120 130 140 150
GSDGTDLFDQ VHRWVNYESM LKECLVGRMA MKPALPKDYH EEKKVLNGML
160 170 180 190 200
PQSQVTDTLA KEGPSSPSYD WFQTDSLVTI VIYTKQKDIN LDSVIVDHRD
210 220 230 240 250
DSFRAETVIK DYSYLVHVAL SHEIQEDFSV LVVENVGKIE IVLKKKENTS
260 270 280 290 300
WKCLGHPQEN HNSFIPKKDT GLFYRKCQLV SKEDVTHDTK LFCLMLPPST
310 320 330 340 350
HLEVPVGQHV YLRLPITGTE IVKPYTPVCD SLFSEFKEPV LPNNIYIYFL
360 370 380 390 400
IKIYPAGFFT PELDQLQIGD YVSVSNPEGN FIISQLQELE DLFLLAAGTG
410 420 430 440 450
FTPMVKVLNY ALTNIPSLRK VKLMFFNKTE DDIIWRSQLE KLAFKDKRFE
460 470 480 490 500
VEFVLSAPTS EWSGKQGYIS PALLSEFLKR RSDTSKVLIC LCGPTPFTEQ
510 520
GMKMLHDLNF SKDEIHSFTA
Length:520
Mass (Da):59,274
Last modified:December 6, 2005 - v1
Checksum:iD855FF27A7A9AF2C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109569 mRNA. Translation: AAI09570.1.
RefSeqiNP_001033248.1. NM_001038159.1.
UniGeneiBt.20479.

Genome annotation databases

GeneIDi533608.
KEGGibta:533608.

Similar proteinsi

Entry informationi

Entry nameiNB5R4_BOVIN
AccessioniPrimary (citable) accession number: Q32LH7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: December 6, 2005
Last modified: October 25, 2017
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families