Reviewed, UniProtKB/Swiss-Prot Q32LH7 (NB5R4_BOVIN)

Names and origin

Protein names

Recommended name:
    Cytochrome b5 reductase 4
    EC=1.6.2.2
Alternative name(s):
    Flavohemoprotein b5/b5R
      Short name=b5+b5R
    cb5/cb5R

Gene names

Name:

CYB5R4

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	NADH-cytochrome b5 reductase involved in endolasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS) By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Endoplasmic reticulum. Note: Soluble protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 CS domain. Contains 1 cytochrome b5 heme-binding domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Ligand	FAD Flavoprotein Heme Iron Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Cytochrome b5 reductase 4		PRO_0000287555
Regions
	Domain	54 – 130	77	Cytochrome b5 heme-binding
	Domain	164 – 255	92	CS
	Domain	272 – 384	113	FAD-binding FR-type
	Nucleotide binding	364 – 379	16	FAD By similarity
	Nucleotide binding	391 – 423	33	FAD By similarity
Sites
	Metal binding	89	1	Iron (heme axial ligand) By similarity
	Metal binding	112	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q32LH7-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: D855FF27A7A9AF2C	FASTA	520	59,274
	10 20 30 40 50 60 MLNVPSQSFP GPSSQQRVAS GGRSKVPLKQ GRSLMDWIRL TKSGKDLTGL KGRLIEVTEE 70 80 90 100 110 120 ELKKHNKKDD CWICIRGFVY NVSPYMEYHP GGEDELMRAA GSDGTDLFDQ VHRWVNYESM 130 140 150 160 170 180 LKECLVGRMA MKPALPKDYH EEKKVLNGML PQSQVTDTLA KEGPSSPSYD WFQTDSLVTI 190 200 210 220 230 240 VIYTKQKDIN LDSVIVDHRD DSFRAETVIK DYSYLVHVAL SHEIQEDFSV LVVENVGKIE 250 260 270 280 290 300 IVLKKKENTS WKCLGHPQEN HNSFIPKKDT GLFYRKCQLV SKEDVTHDTK LFCLMLPPST 310 320 330 340 350 360 HLEVPVGQHV YLRLPITGTE IVKPYTPVCD SLFSEFKEPV LPNNIYIYFL IKIYPAGFFT 370 380 390 400 410 420 PELDQLQIGD YVSVSNPEGN FIISQLQELE DLFLLAAGTG FTPMVKVLNY ALTNIPSLRK 430 440 450 460 470 480 VKLMFFNKTE DDIIWRSQLE KLAFKDKRFE VEFVLSAPTS EWSGKQGYIS PALLSEFLKR 490 500 510 520 RSDTSKVLIC LCGPTPFTEQ GMKMLHDLNF SKDEIHSFTA

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC109569 mRNA. Translation: AAI09570.1.
IPI	IPI00685168.
RefSeq	NP_001033248.1.
UniGene	Bt.20479
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000013591. Bos taurus. [Contig view]
GeneID	533608.
KEGG	bta:533608.
Phylogenomic databases
HOVERGEN	Q32LH7.
Enzyme and pathway databases
BRENDA	1.6.2.2. 251.
Family and domain databases
InterPro	IPR017447. CS. IPR007052. CS_domain. IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR017927. Fd_Rdtase_FAD-bd. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF04969. CS. 1 hit. PF00173. Cyt-b5. 1 hit. PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00363. CYTOCHROMEB5.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS51203. CS. 1 hit. PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NB5R4_BOVIN

Accession

Primary (citable) accession number: Q32LH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	December 6, 2005
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)