Reviewed, UniProtKB/Swiss-Prot Q32LE5 (ASGL1_BOVIN)

Names and origin

Protein names

Recommended name:
    L-asparaginase
    EC=3.5.1.1
Alternative name(s):
    L-asparagine amidohydrolase
    Asparaginase-like protein 1

Gene names

Name:

ASRGL1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts in asparagine catabolism. May be involved in astroglial production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions By similarity.
Catalytic activity	L-asparagine + H2O = L-aspartate + NH3.
Subcellular location	Cytoplasm By similarity. Note: Midpiece of sperm tail By similarity.
Sequence similarities	Belongs to the Ntn-hydrolase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Acetylation
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	asparaginase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	L-asparaginase		PRO_0000305203
Sites
	Active site	168	1	Nucleophile By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q32LE5-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: F17066092C340EE4	FASTA	308	32,050
	10 20 30 40 50 60 MNPVVVVHGG GASNISKDRK ERVRQGILRA ATVGYNILKQ GGSAVDAVEG AVTVLEDDPD 70 80 90 100 110 120 FNAGCGSVLN ENGEVEMDAS IMNGKDLSAG AVSAVRCIAN PIKLARLVMD KTPHCFLTDQ 130 140 150 160 170 180 GAARFAAANG IPTIPGQQLV TERSRKRLEK EKLEKDAQKP DCQKNLGTVG AVALDCQGNL 190 200 210 220 230 240 AYATSTGGIV NKMPGRVGDT PCVGSGGYAD NDIGAVSTTG HGESILKVNL ARLALFHVEQ 250 260 270 280 290 300 GKSLEEAANA SLGHMKSKVK GVGGIIMVNK AGEWAVKWTS TSMPWAAAKD GKLHSGIDFG DTSIIDLS

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC109621 mRNA. Translation: AAI09622.1.
IPI	IPI00714193.
RefSeq	NP_001070503.1.
UniGene	Bt.685
3D structure databases
SMR	Q32LE5. Positions 3-297.
ModBase	Search...
Protein-protein interaction databases
STRING	Q32LE5.
Proteomic databases
PRIDE	Q32LE5.
Genome annotation databases
Ensembl	ENSBTAT00000009073; ENSBTAP00000009073; ENSBTAG00000006910; Bos taurus. [Genome view]
GeneID	767970.
KEGG	bta:767970.
Organism-specific databases
CTD	767970.
Phylogenomic databases
eggNOG	maNOG15100.
HOVERGEN	Q32LE5.
InParanoid	Q32LE5.
PhylomeDB	Q32LE5.
Enzyme and pathway databases
BRENDA	3.5.1.1. 251.
Family and domain databases
InterPro	IPR000246. Peptidase_T2. [Graphical view]
PANTHER	PTHR10188. Peptidase_T2. 1 hit.
Pfam	PF01112. Asparaginase_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASGL1_BOVIN

Accession

Primary (citable) accession number: Q32LE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	December 6, 2005
Last modified:	January 19, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)