ID   GKAP1_BOVIN             Reviewed;         363 AA.
AC   Q32LE2;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 2.
DT   24-JAN-2024, entry version 88.
DE   RecName: Full=G kinase-anchoring protein 1;
GN   Name=GKAP1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Regulates insulin-dependent IRS1 tyrosine phosphorylation in
CC       adipocytes by modulating the availability of IRS1 to IR tyrosine
CC       kinase. Its association with IRS1 is required for insulin-induced
CC       translocation of SLC2A4 to the cell membrane. Involved in TNF-induced
CC       impairment of insulin-dependent IRS1 tyrosine phosphorylation.
CC       {ECO:0000250|UniProtKB:Q9JMB0}.
CC   -!- SUBUNIT: Interacts with PRKG1 and IRS1. {ECO:0000250|UniProtKB:Q9JMB0}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000250|UniProtKB:Q9JMB0}.
CC   -!- SIMILARITY: Belongs to the GKAP1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAI09626.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; BC109625; AAI09626.1; ALT_FRAME; mRNA.
DR   RefSeq; NP_001035663.2; NM_001040573.3.
DR   AlphaFoldDB; Q32LE2; -.
DR   SMR; Q32LE2; -.
DR   STRING; 9913.ENSBTAP00000070991; -.
DR   PaxDb; 9913-ENSBTAP00000012912; -.
DR   Ensembl; ENSBTAT00000081827.1; ENSBTAP00000070991.1; ENSBTAG00000023523.5.
DR   GeneID; 613960; -.
DR   KEGG; bta:613960; -.
DR   CTD; 80318; -.
DR   VEuPathDB; HostDB:ENSBTAG00000023523; -.
DR   VGNC; VGNC:29387; GKAP1.
DR   eggNOG; ENOG502QUT6; Eukaryota.
DR   GeneTree; ENSGT00390000008742; -.
DR   HOGENOM; CLU_065161_1_0_1; -.
DR   InParanoid; Q32LE2; -.
DR   OMA; RKNHQGR; -.
DR   OrthoDB; 5353928at2759; -.
DR   TreeFam; TF328459; -.
DR   Proteomes; UP000009136; Chromosome 8.
DR   Bgee; ENSBTAG00000023523; Expressed in spermatid and 105 other cell types or tissues.
DR   ExpressionAtlas; Q32LE2; baseline and differential.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
DR   GO; GO:0046628; P:positive regulation of insulin receptor signaling pathway; ISS:UniProtKB.
DR   GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR   Gene3D; 1.20.5.170; -; 1.
DR   InterPro; IPR026109; GKAP1.
DR   PANTHER; PTHR14899; G KINASE ANCHORING PROTEIN 1; 1.
DR   PANTHER; PTHR14899:SF0; G KINASE-ANCHORING PROTEIN 1; 1.
DR   PRINTS; PR02083; GKINASEAP1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Golgi apparatus; Phosphoprotein; Reference proteome.
FT   CHAIN           1..363
FT                   /note="G kinase-anchoring protein 1"
FT                   /id="PRO_0000315653"
FT   REGION          1..93
FT                   /note="Interaction with IRS1"
FT                   /evidence="ECO:0000250|UniProtKB:Q9JMB0"
FT   REGION          20..111
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          145..171
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          43..75
FT                   /evidence="ECO:0000255"
FT   COILED          126..158
FT                   /evidence="ECO:0000255"
FT   COILED          240..350
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        84..103
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         23
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9JMB0"
FT   MOD_RES         25
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9JMB0"
FT   MOD_RES         27
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5VSY0"
FT   MOD_RES         104
FT                   /note="Phosphoserine; by PKG"
FT                   /evidence="ECO:0000250|UniProtKB:Q9JMB0"
SQ   SEQUENCE   363 AA;  41881 MW;  368D6EBE6FA940EB CRC64;
     MASAVLSSVP TTASRFALLQ VDSGSGSDSE PGKGKGRNTG KSQTNKSTTN EKKREKRRKK
     KEQQQSEANE LRNLAFKKIP QKSSHAICNA QHELSLPNPV QKDSREENWQ EWRQRDEQLT
     SEMFEADLEK ALLLSKLEYE EHKKEYENAE NASTQSKVMN KKDKRKTHQG KDKPLTISLK
     EFQSEDHISK KTEELSSQIL SHDGGFFNRL EDDVHKILTR EKRREQLTEY NGTDNYTVHE
     HNQEVVLKDG RIERLKLELE RKDAEIQKLK NVIAQWEAKY KEVKARNAQL LKMLQEGEMK
     DKAEILLQVD ESQSIKNELT IQVTSLHAAL EQERSKVKVL QAELAKYQGG RKGKRNSESD
     QCR
//