Q32LD2 (UBE2T_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 T
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein T
Ubiquitin-protein ligase T

Gene names

Name:

UBE2T

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with FANCL and BRCA1 By similarity.
Subcellular location	Nucleus By similarity. Note: Accumulates to chromatin By similarity.
Post-translational modification	Auto-ubiquitinated. Effects of auto-monoubiquitination at Lys-91 and Lys-180 are unclear By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from sequence or structural similarity. Source: UniProtKB protein K11-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K27-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K29-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K48-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K6-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein autoubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein monoubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW chromatin binding Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 195	195	Ubiquitin-conjugating enzyme E2 T		PRO_0000281863
Sites
	Active site	86	1	Glycyl thioester intermediate By similarity
Amino acid modifications
	Cross-link	91		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	180		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Experimental info
	Sequence conflict	110	1	S → C in AAI09638. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q32LD2 [UniParc]. Last modified April 18, 2012. Version 2. Checksum: 191CF2C9F50CE22B	FASTA	195	21,765
	10 20 30 40 50 60 MQRTSRLKRE LSLLAAEPPP GITCWQDGDR MEDLRAQILG GANTPYEKGV FKLEVHIPER 70 80 90 100 110 120 YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP SLNIATLLTS IQQLMAEPNP 130 140 150 160 170 180 DDPLMADISS EFKYNKPVFF KNARQWTEKH ARQKTDEEGM PGSLPEVGGS EGPSAAQKRK 190 AGQLSSGGKR FCPDV

References

[1]	"The genome sequence of taurine cattle: a window to ruminant biology and evolution." The bovine genome sequencing and analysis consortium Science 324:522-528(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hereford.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DAAA02043707 Genomic DNA. No translation available. BC109637 mRNA. Translation: AAI09638.1.
IPI	IPI00718387.
RefSeq	NP_001033123.1. NM_001038034.2.
UniGene	Bt.2915.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000006290.
Proteomic databases
PRIDE	Q32LD2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000006290; ENSBTAP00000006290; ENSBTAG00000004790.
GeneID	505314.
KEGG	bta:505314.
Organism-specific databases
CTD	29089.
Phylogenomic databases
eggNOG	COG5078.
GeneTree	ENSGT00540000070023.
HOGENOM	HOG000233455.
HOVERGEN	HBG063308.
InParanoid	Q32LD2.
KO	K13960.
OMA	GITCWQE.
OrthoDB	EOG40GCRT.
Enzyme and pathway databases
BioCyc	CATTLE:505314-MONOMER.
UniPathway	UPA00143.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20867078.

Entry information

Entry name

UBE2T_BOVIN

Accession

Primary (citable) accession number: Q32LD2
Secondary accession number(s): G3X6G9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 18, 2012
Last modified:	May 29, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program