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Q32LD2

- UBE2T_BOVIN

UniProt

Q32LD2 - UBE2T_BOVIN

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Protein
Ubiquitin-conjugating enzyme E2 T
Gene
UBE2T
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination By similarity.

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei86 – 861Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. acid-amino acid ligase activity Source: InterPro
  3. chromatin binding Source: UniProtKB
  4. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. DNA repair Source: UniProtKB
  2. cellular response to DNA damage stimulus Source: UniProtKB
  3. protein K11-linked ubiquitination Source: UniProtKB
  4. protein K27-linked ubiquitination Source: UniProtKB
  5. protein K29-linked ubiquitination Source: UniProtKB
  6. protein K48-linked ubiquitination Source: UniProtKB
  7. protein K6-linked ubiquitination Source: UniProtKB
  8. protein K63-linked ubiquitination Source: UniProtKB
  9. protein autoubiquitination Source: UniProtKB
  10. protein monoubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_205389. Fanconi Anemia pathway.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 T (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein T
Ubiquitin-protein ligase T
Gene namesi
Name:UBE2T
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 16

Subcellular locationi

Nucleus By similarity
Note: Accumulates to chromatin By similarity.

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 195195Ubiquitin-conjugating enzyme E2 T
PRO_0000281863Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki91 – 91Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-linki180 – 180Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Post-translational modificationi

Auto-ubiquitinated. Effects of auto-monoubiquitination at Lys-91 and Lys-180 are unclear By similarity.

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PRIDEiQ32LD2.

Interactioni

Subunit structurei

Interacts with FANCL and BRCA1 By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006290.

Structurei

3D structure databases

ProteinModelPortaliQ32LD2.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00540000070023.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ32LD2.
KOiK13960.
OMAiIVPERYP.
OrthoDBiEOG7BP84K.
TreeFamiTF354203.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q32LD2-1 [UniParc]FASTAAdd to Basket

« Hide

MQRTSRLKRE LSLLAAEPPP GITCWQDGDR MEDLRAQILG GANTPYEKGV    50
FKLEVHIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP 100
SLNIATLLTS IQQLMAEPNP DDPLMADISS EFKYNKPVFF KNARQWTEKH 150
ARQKTDEEGM PGSLPEVGGS EGPSAAQKRK AGQLSSGGKR FCPDV 195
Length:195
Mass (Da):21,765
Last modified:April 18, 2012 - v2
Checksum:i191CF2C9F50CE22B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1101S → C in AAI09638. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DAAA02043707 Genomic DNA. No translation available.
BC109637 mRNA. Translation: AAI09638.1.
RefSeqiNP_001033123.1. NM_001038034.2.
XP_005217388.1. XM_005217331.1.
UniGeneiBt.2915.

Genome annotation databases

EnsembliENSBTAT00000006290; ENSBTAP00000006290; ENSBTAG00000004790.
GeneIDi505314.
KEGGibta:505314.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DAAA02043707 Genomic DNA. No translation available.
BC109637 mRNA. Translation: AAI09638.1 .
RefSeqi NP_001033123.1. NM_001038034.2.
XP_005217388.1. XM_005217331.1.
UniGenei Bt.2915.

3D structure databases

ProteinModelPortali Q32LD2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000006290.

Proteomic databases

PRIDEi Q32LD2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000006290 ; ENSBTAP00000006290 ; ENSBTAG00000004790 .
GeneIDi 505314.
KEGGi bta:505314.

Organism-specific databases

CTDi 29089.

Phylogenomic databases

eggNOGi COG5078.
GeneTreei ENSGT00540000070023.
HOGENOMi HOG000233455.
HOVERGENi HBG063308.
InParanoidi Q32LD2.
KOi K13960.
OMAi IVPERYP.
OrthoDBi EOG7BP84K.
TreeFami TF354203.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_205389. Fanconi Anemia pathway.

Miscellaneous databases

NextBioi 20867078.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hereford.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiUBE2T_BOVIN
AccessioniPrimary (citable) accession number: Q32LD2
Secondary accession number(s): G3X6G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 18, 2012
Last modified: September 3, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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