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Q32LD2 (UBE2T_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 T

EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein T
Ubiquitin-protein ligase T
Gene names
Name:UBE2T
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length195 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination By similarity.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with FANCL and BRCA1 By similarity.

Subcellular location

Nucleus By similarity. Note: Accumulates to chromatin By similarity.

Post-translational modification

Auto-ubiquitinated. Effects of auto-monoubiquitination at Lys-91 and Lys-180 are unclear By similarity.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
Ubl conjugation pathway
   Cellular componentNucleus
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   PTMIsopeptide bond
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA repair

Inferred from sequence or structural similarity. Source: UniProtKB

cellular response to DNA damage stimulus

Inferred from sequence or structural similarity. Source: UniProtKB

protein K11-linked ubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein K27-linked ubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein K29-linked ubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein K48-linked ubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein K6-linked ubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein K63-linked ubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein autoubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein monoubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

chromatin binding

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin-protein ligase activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 195195Ubiquitin-conjugating enzyme E2 T
PRO_0000281863

Sites

Active site861Glycyl thioester intermediate By similarity

Amino acid modifications

Cross-link91Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-link180Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Experimental info

Sequence conflict1101S → C in AAI09638. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q32LD2 [UniParc].

Last modified April 18, 2012. Version 2.
Checksum: 191CF2C9F50CE22B

FASTA19521,765
        10         20         30         40         50         60 
MQRTSRLKRE LSLLAAEPPP GITCWQDGDR MEDLRAQILG GANTPYEKGV FKLEVHIPER 

        70         80         90        100        110        120 
YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP SLNIATLLTS IQQLMAEPNP 

       130        140        150        160        170        180 
DDPLMADISS EFKYNKPVFF KNARQWTEKH ARQKTDEEGM PGSLPEVGGS EGPSAAQKRK 

       190 
AGQLSSGGKR FCPDV 

« Hide

References

[1]"A whole-genome assembly of the domestic cow, Bos taurus."
Zimin A.V., Delcher A.L., Florea L., Kelley D.R., Schatz M.C., Puiu D., Hanrahan F., Pertea G., Van Tassell C.P., Sonstegard T.S., Marcais G., Roberts M., Subramanian P., Yorke J.A., Salzberg S.L.
Genome Biol. 10:R42.01-R42.10(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Hereford.
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DAAA02043707 Genomic DNA. No translation available.
BC109637 mRNA. Translation: AAI09638.1.
RefSeqNP_001033123.1. NM_001038034.2.
XP_005217388.1. XM_005217331.1.
UniGeneBt.2915.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000006290.

Proteomic databases

PRIDEQ32LD2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000006290; ENSBTAP00000006290; ENSBTAG00000004790.
GeneID505314.
KEGGbta:505314.

Organism-specific databases

CTD29089.

Phylogenomic databases

eggNOGCOG5078.
GeneTreeENSGT00540000070023.
HOGENOMHOG000233455.
HOVERGENHBG063308.
InParanoidQ32LD2.
KOK13960.
OMAELHMLAT.
OrthoDBEOG7BP84K.
TreeFamTF354203.

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20867078.

Entry information

Entry nameUBE2T_BOVIN
AccessionPrimary (citable) accession number: Q32LD2
Secondary accession number(s): G3X6G9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 18, 2012
Last modified: April 16, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways