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Protein

Ubiquitin-conjugating enzyme E2 T

Gene

UBE2T

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.By similarity

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processDNA damage, DNA repair, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-6783310. Fanconi Anemia Pathway.
R-BTA-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 T (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme T
Ubiquitin carrier protein T
Ubiquitin-protein ligase T
Gene namesi
Name:UBE2T
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 16

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002818631 – 195Ubiquitin-conjugating enzyme E2 TAdd BLAST195

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki91Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki180Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki189Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Auto-ubiquitinated. Effects of auto-monoubiquitination at Lys-91 and Lys-180 are unclear.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ32LD2.
PRIDEiQ32LD2.

Expressioni

Gene expression databases

BgeeiENSBTAG00000004790.

Interactioni

Subunit structurei

Interacts with FANCL and BRCA1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006290.

Structurei

3D structure databases

ProteinModelPortaliQ32LD2.
SMRiQ32LD2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00540000070023.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ32LD2.
KOiK13960.
OMAiKIPERYP.
OrthoDBiEOG091G0VPD.
TreeFamiTF354203.

Family and domain databases

CDDicd00195. UBCc. 1 hit.
Gene3Di3.10.110.10. 1 hit.
InterProiView protein in InterPro
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
PfamiView protein in Pfam
PF00179. UQ_con. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q32LD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRTSRLKRE LSLLAAEPPP GITCWQDGDR MEDLRAQILG GANTPYEKGV
60 70 80 90 100
FKLEVHIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP
110 120 130 140 150
SLNIATLLTS IQQLMAEPNP DDPLMADISS EFKYNKPVFF KNARQWTEKH
160 170 180 190
ARQKTDEEGM PGSLPEVGGS EGPSAAQKRK AGQLSSGGKR FCPDV
Length:195
Mass (Da):21,765
Last modified:April 18, 2012 - v2
Checksum:i191CF2C9F50CE22B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti110S → C in AAI09638 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02043707 Genomic DNA. No translation available.
BC109637 mRNA. Translation: AAI09638.1.
RefSeqiNP_001033123.1. NM_001038034.2.
UniGeneiBt.2915.

Genome annotation databases

EnsembliENSBTAT00000006290; ENSBTAP00000006290; ENSBTAG00000004790.
GeneIDi505314.
KEGGibta:505314.

Similar proteinsi

Entry informationi

Entry nameiUBE2T_BOVIN
AccessioniPrimary (citable) accession number: Q32LD2
Secondary accession number(s): G3X6G9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 18, 2012
Last modified: August 30, 2017
This is version 93 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families