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Q32LD2

- UBE2T_BOVIN

UniProt

Q32LD2 - UBE2T_BOVIN

Protein

Ubiquitin-conjugating enzyme E2 T

Gene

UBE2T

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 2 (18 Apr 2012)
      Previous versions | rss
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    Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination By similarity.By similarity

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei86 – 861Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. chromatin binding Source: UniProtKB
    4. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: UniProtKB
    2. DNA repair Source: UniProtKB
    3. protein autoubiquitination Source: UniProtKB
    4. protein K11-linked ubiquitination Source: UniProtKB
    5. protein K27-linked ubiquitination Source: UniProtKB
    6. protein K29-linked ubiquitination Source: UniProtKB
    7. protein K48-linked ubiquitination Source: UniProtKB
    8. protein K63-linked ubiquitination Source: UniProtKB
    9. protein K6-linked ubiquitination Source: UniProtKB
    10. protein monoubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    DNA damage, DNA repair, Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_205389. Fanconi Anemia pathway.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 T (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein T
    Ubiquitin-protein ligase T
    Gene namesi
    Name:UBE2T
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 16

    Subcellular locationi

    Nucleus By similarity
    Note: Accumulates to chromatin.By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 195195Ubiquitin-conjugating enzyme E2 TPRO_0000281863Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki91 – 91Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki180 – 180Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

    Post-translational modificationi

    Auto-ubiquitinated. Effects of auto-monoubiquitination at Lys-91 and Lys-180 are unclear By similarity.By similarity

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    PRIDEiQ32LD2.

    Interactioni

    Subunit structurei

    Interacts with FANCL and BRCA1.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000006290.

    Structurei

    3D structure databases

    ProteinModelPortaliQ32LD2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00540000070023.
    HOGENOMiHOG000233455.
    HOVERGENiHBG063308.
    InParanoidiQ32LD2.
    KOiK13960.
    OMAiIVPERYP.
    OrthoDBiEOG7BP84K.
    TreeFamiTF354203.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q32LD2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQRTSRLKRE LSLLAAEPPP GITCWQDGDR MEDLRAQILG GANTPYEKGV    50
    FKLEVHIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP 100
    SLNIATLLTS IQQLMAEPNP DDPLMADISS EFKYNKPVFF KNARQWTEKH 150
    ARQKTDEEGM PGSLPEVGGS EGPSAAQKRK AGQLSSGGKR FCPDV 195
    Length:195
    Mass (Da):21,765
    Last modified:April 18, 2012 - v2
    Checksum:i191CF2C9F50CE22B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti110 – 1101S → C in AAI09638. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DAAA02043707 Genomic DNA. No translation available.
    BC109637 mRNA. Translation: AAI09638.1.
    RefSeqiNP_001033123.1. NM_001038034.2.
    XP_005217388.1. XM_005217331.1.
    UniGeneiBt.2915.

    Genome annotation databases

    EnsembliENSBTAT00000006290; ENSBTAP00000006290; ENSBTAG00000004790.
    GeneIDi505314.
    KEGGibta:505314.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DAAA02043707 Genomic DNA. No translation available.
    BC109637 mRNA. Translation: AAI09638.1 .
    RefSeqi NP_001033123.1. NM_001038034.2.
    XP_005217388.1. XM_005217331.1.
    UniGenei Bt.2915.

    3D structure databases

    ProteinModelPortali Q32LD2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000006290.

    Proteomic databases

    PRIDEi Q32LD2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000006290 ; ENSBTAP00000006290 ; ENSBTAG00000004790 .
    GeneIDi 505314.
    KEGGi bta:505314.

    Organism-specific databases

    CTDi 29089.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00540000070023.
    HOGENOMi HOG000233455.
    HOVERGENi HBG063308.
    InParanoidi Q32LD2.
    KOi K13960.
    OMAi IVPERYP.
    OrthoDBi EOG7BP84K.
    TreeFami TF354203.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_205389. Fanconi Anemia pathway.

    Miscellaneous databases

    NextBioi 20867078.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hereford.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Liver.

    Entry informationi

    Entry nameiUBE2T_BOVIN
    AccessioniPrimary (citable) accession number: Q32LD2
    Secondary accession number(s): G3X6G9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: April 18, 2012
    Last modified: October 1, 2014
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3