Q32L99 (PTGR2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prostaglandin reductase 2
Short name=PRG-2
EC=1.3.1.48

Alternative name(s):
15-oxoprostaglandin 13-reductase

Gene names

Name:

PTGR2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation By similarity.
Catalytic activity	11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NADP-dependent oxidoreductase L4BD family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	prostaglandin metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	15-oxoprostaglandin 13-oxidase activity Inferred from sequence or structural similarity. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Prostaglandin reductase 2		PRO_0000285789
Regions
	Nucleotide binding	165 – 168	4	NADP By similarity
	Nucleotide binding	253 – 259	7	NADP By similarity
	Nucleotide binding	287 – 289	3	NADP By similarity
	Region	99 – 100	2	Substrate binding By similarity
	Region	288 – 290	3	Substrate binding By similarity
Sites
	Binding site	192	1	NADP By similarity
	Binding site	208	1	NADP By similarity
	Binding site	231	1	NADP By similarity
	Binding site	337	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q32L99 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 6BC981F3DDA5A9B8	FASTA	351	38,400
	10 20 30 40 50 60 MIVQRVVLNS RPGKNGHPVA ENFRVEEVNL PDCVNEGQVQ VRTLYLSVDP YMRCRMNEDT 70 80 90 100 110 120 GSDYITPWQL SQVVDGGGVG IIEESKHTNF MKGDFVTSFY WPWQTKVILD GNILEKVDPQ 130 140 150 160 170 180 LVDGHLSYFL GAIGMPGLTS LIGVQEKGHI TAGSNQTMVV SGAAGACGSL AGQIGRLLGC 190 200 210 220 230 240 SRVVGICGTP EKCLFLTSEL GFDAAINYKE GNVAEQLHKL CPAGVDVYFD NVGGDISDTV 250 260 270 280 290 300 ISQMNQNSHI ILCGQISQYN KDVPYPPPLP PAIEAIQKER NITRERFLVL NYKDKFEFGI 310 320 330 340 350 LQLSQWFKEG KLKIKETMIN GLENMGAAFQ SMMTGGNIGK QIVCISGDTS L

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC109688 mRNA. Translation: AAI09689.1.
IPI	IPI00687791.
RefSeq	NP_001068717.1. NM_001075249.1.
UniGene	Bt.38238.
3D structure databases
ProteinModelPortal	Q32L99.
SMR	Q32L99. Positions 1-351.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000004878; ENSBTAP00000004878; ENSBTAG00000003747.
GeneID	506263.
KEGG	bta:506263.
Organism-specific databases
CTD	145482.
Phylogenomic databases
eggNOG	maNOG10795.
GeneTree	ENSGT00390000009335.
HOVERGEN	HBG055024.
InParanoid	Q32L99.
OMA	HELCPAG.
OrthoDB	EOG4S4PGG.
PhylomeDB	Q32L99.
Family and domain databases
InterPro	IPR013149. ADH_C. IPR002085. ADH_SF_Zn-type. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K13949.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 2 hits.
ProtoNet	Search...

Entry information

Entry name

PTGR2_BOVIN

Accession

Primary (citable) accession number: Q32L99

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	December 6, 2005
Last modified:	December 14, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program