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Q32L67

- GLRX2_BOVIN

UniProt

Q32L67 - GLRX2_BOVIN

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Protein
Glutaredoxin-2, mitochondrial
Gene
GLRX2
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release By similarity.

Enzyme regulationi

The 2Fe-2S present in the homodimer leads to inactivation of the enzyme. The 2Fe-2S may serve as a redox sensor: the presence of one-electron oxidants or reductants leading to the loss of the 2Fe-2S cluster, subsequent monomerization and activation of the enzyme By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Iron-sulfur (2Fe-2S); shared with dimeric partner; in inactive form By similarity
Binding sitei68 – 681Glutathione By similarity
Binding sitei103 – 1031Glutathione By similarity
Binding sitei115 – 1151Glutathione; via amide nitrogen and carbonyl oxygen By similarity
Metal bindingi147 – 1471Iron-sulfur (2Fe-2S); shared with dimeric partner; in inactive form By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. electron carrier activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. protein disulfide oxidoreductase activity Source: InterPro

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
  2. response to hydrogen peroxide Source: Ensembl
  3. response to organic substance Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaredoxin-2, mitochondrial
Gene namesi
Name:GLRX2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 16

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
  2. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1818Mitochondrion Reviewed prediction
Add
BLAST
Chaini19 – 157139Glutaredoxin-2, mitochondrial
PRO_0000326628Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi71 ↔ 74Redox-active; alternate By similarity
Modified residuei71 – 711S-glutathionyl cysteine; alternate By similarity

Keywords - PTMi

Disulfide bond, Glutathionylation

Interactioni

Subunit structurei

Monomer; active form. Homodimer; inactive form. The homodimer is probably linked by 1 2Fe-2S cluster By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000021249.

Structurei

3D structure databases

ProteinModelPortaliQ32L67.
SMRiQ32L67. Positions 43-157.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 151101Glutaredoxin
Add
BLAST

Sequence similaritiesi

Belongs to the glutaredoxin family.

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiCOG0695.
GeneTreeiENSGT00390000003677.
HOGENOMiHOG000095204.
HOVERGENiHBG096801.
InParanoidiQ32L67.
KOiK03676.
OMAiGMESNTS.
OrthoDBiEOG7QRQWZ.
TreeFamiTF319627.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR002109. Glutaredoxin.
IPR011899. Glutaredoxin_euk/vir.
IPR014025. Glutaredoxin_subgr.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00462. Glutaredoxin. 1 hit.
[Graphical view]
PRINTSiPR00160. GLUTAREDOXIN.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR02180. GRX_euk. 1 hit.
PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q32L67-1 [UniParc]FASTAAdd to Basket

« Hide

MYWRRAALVG TRLIPVRSSS AGRLEGPAGI SGSGMGNSTS SSLGNAATAP    50
VNQIQETISN NCVVIFSKTS CSYCTMAKNL FHDMNVNYKV VELDMLEYGS 100
QFQDALHKMT GERTVPRIFV NGTFIGGATD THRLHKEGKL LPLVHQCHLK 150
NSKREEL 157
Length:157
Mass (Da):17,202
Last modified:December 6, 2005 - v1
Checksum:i3106F9214733EFD1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC109742 mRNA. Translation: AAI09743.1.
RefSeqiNP_001035613.1. NM_001040523.2.
XP_005216824.1. XM_005216767.1.
UniGeneiBt.27603.

Genome annotation databases

EnsembliENSBTAT00000021249; ENSBTAP00000021249; ENSBTAG00000015972.
GeneIDi513762.
KEGGibta:513762.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC109742 mRNA. Translation: AAI09743.1 .
RefSeqi NP_001035613.1. NM_001040523.2.
XP_005216824.1. XM_005216767.1.
UniGenei Bt.27603.

3D structure databases

ProteinModelPortali Q32L67.
SMRi Q32L67. Positions 43-157.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000021249.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000021249 ; ENSBTAP00000021249 ; ENSBTAG00000015972 .
GeneIDi 513762.
KEGGi bta:513762.

Organism-specific databases

CTDi 51022.

Phylogenomic databases

eggNOGi COG0695.
GeneTreei ENSGT00390000003677.
HOGENOMi HOG000095204.
HOVERGENi HBG096801.
InParanoidi Q32L67.
KOi K03676.
OMAi GMESNTS.
OrthoDBi EOG7QRQWZ.
TreeFami TF319627.

Miscellaneous databases

NextBioi 20871016.

Family and domain databases

Gene3Di 3.40.30.10. 1 hit.
InterProi IPR002109. Glutaredoxin.
IPR011899. Glutaredoxin_euk/vir.
IPR014025. Glutaredoxin_subgr.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00462. Glutaredoxin. 1 hit.
[Graphical view ]
PRINTSi PR00160. GLUTAREDOXIN.
SUPFAMi SSF52833. SSF52833. 1 hit.
TIGRFAMsi TIGR02180. GRX_euk. 1 hit.
PROSITEi PS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiGLRX2_BOVIN
AccessioniPrimary (citable) accession number: Q32L67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: December 6, 2005
Last modified: June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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