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Q32L67

- GLRX2_BOVIN

UniProt

Q32L67 - GLRX2_BOVIN

Protein

Glutaredoxin-2, mitochondrial

Gene

GLRX2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release By similarity.By similarity

    Enzyme regulationi

    The 2Fe-2S present in the homodimer leads to inactivation of the enzyme. The 2Fe-2S may serve as a redox sensor: the presence of one-electron oxidants or reductants leading to the loss of the 2Fe-2S cluster, subsequent monomerization and activation of the enzyme By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi62 – 621Iron-sulfur (2Fe-2S); shared with dimeric partner; in inactive formBy similarity
    Binding sitei68 – 681GlutathioneBy similarity
    Binding sitei103 – 1031GlutathioneBy similarity
    Binding sitei115 – 1151Glutathione; via amide nitrogen and carbonyl oxygenBy similarity
    Metal bindingi147 – 1471Iron-sulfur (2Fe-2S); shared with dimeric partner; in inactive formBy similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. electron carrier activity Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. protein disulfide oxidoreductase activity Source: InterPro

    GO - Biological processi

    1. cell redox homeostasis Source: InterPro
    2. response to hydrogen peroxide Source: Ensembl
    3. response to organic substance Source: Ensembl

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutaredoxin-2, mitochondrial
    Gene namesi
    Name:GLRX2
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 16

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell
    2. nucleus Source: Ensembl

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1818MitochondrionSequence AnalysisAdd
    BLAST
    Chaini19 – 157139Glutaredoxin-2, mitochondrialPRO_0000326628Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi71 ↔ 74Redox-active; alternateBy similarity
    Modified residuei71 – 711S-glutathionyl cysteine; alternateBy similarity

    Keywords - PTMi

    Disulfide bond, Glutathionylation

    Interactioni

    Subunit structurei

    Monomer; active form. Homodimer; inactive form. The homodimer is probably linked by 1 2Fe-2S cluster By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000021249.

    Structurei

    3D structure databases

    ProteinModelPortaliQ32L67.
    SMRiQ32L67. Positions 43-157.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini51 – 151101GlutaredoxinPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glutaredoxin family.Curated
    Contains 1 glutaredoxin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Redox-active center, Transit peptide

    Phylogenomic databases

    eggNOGiCOG0695.
    GeneTreeiENSGT00390000003677.
    HOGENOMiHOG000095204.
    HOVERGENiHBG096801.
    InParanoidiQ32L67.
    KOiK03676.
    OMAiGMESNTS.
    OrthoDBiEOG7QRQWZ.
    TreeFamiTF319627.

    Family and domain databases

    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR002109. Glutaredoxin.
    IPR011899. Glutaredoxin_euk/vir.
    IPR014025. Glutaredoxin_subgr.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00462. Glutaredoxin. 1 hit.
    [Graphical view]
    PRINTSiPR00160. GLUTAREDOXIN.
    SUPFAMiSSF52833. SSF52833. 1 hit.
    TIGRFAMsiTIGR02180. GRX_euk. 1 hit.
    PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q32L67-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYWRRAALVG TRLIPVRSSS AGRLEGPAGI SGSGMGNSTS SSLGNAATAP    50
    VNQIQETISN NCVVIFSKTS CSYCTMAKNL FHDMNVNYKV VELDMLEYGS 100
    QFQDALHKMT GERTVPRIFV NGTFIGGATD THRLHKEGKL LPLVHQCHLK 150
    NSKREEL 157
    Length:157
    Mass (Da):17,202
    Last modified:December 6, 2005 - v1
    Checksum:i3106F9214733EFD1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC109742 mRNA. Translation: AAI09743.1.
    RefSeqiNP_001035613.1. NM_001040523.2.
    XP_005216824.1. XM_005216767.1.
    UniGeneiBt.27603.

    Genome annotation databases

    EnsembliENSBTAT00000021249; ENSBTAP00000021249; ENSBTAG00000015972.
    GeneIDi513762.
    KEGGibta:513762.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC109742 mRNA. Translation: AAI09743.1 .
    RefSeqi NP_001035613.1. NM_001040523.2.
    XP_005216824.1. XM_005216767.1.
    UniGenei Bt.27603.

    3D structure databases

    ProteinModelPortali Q32L67.
    SMRi Q32L67. Positions 43-157.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000021249.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000021249 ; ENSBTAP00000021249 ; ENSBTAG00000015972 .
    GeneIDi 513762.
    KEGGi bta:513762.

    Organism-specific databases

    CTDi 51022.

    Phylogenomic databases

    eggNOGi COG0695.
    GeneTreei ENSGT00390000003677.
    HOGENOMi HOG000095204.
    HOVERGENi HBG096801.
    InParanoidi Q32L67.
    KOi K03676.
    OMAi GMESNTS.
    OrthoDBi EOG7QRQWZ.
    TreeFami TF319627.

    Miscellaneous databases

    NextBioi 20871016.

    Family and domain databases

    Gene3Di 3.40.30.10. 1 hit.
    InterProi IPR002109. Glutaredoxin.
    IPR011899. Glutaredoxin_euk/vir.
    IPR014025. Glutaredoxin_subgr.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00462. Glutaredoxin. 1 hit.
    [Graphical view ]
    PRINTSi PR00160. GLUTAREDOXIN.
    SUPFAMi SSF52833. SSF52833. 1 hit.
    TIGRFAMsi TIGR02180. GRX_euk. 1 hit.
    PROSITEi PS51354. GLUTAREDOXIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Liver.

    Entry informationi

    Entry nameiGLRX2_BOVIN
    AccessioniPrimary (citable) accession number: Q32L67
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3