Q32L27 (UB2Q2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 Q2
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein Q2
Ubiquitin-protein ligase Q2

Gene names

Name:

UBE2Q2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein K48-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Ubiquitin-conjugating enzyme E2 Q2		PRO_0000223878
Regions
	Compositional bias	41 – 55	15	Pro-rich
	Compositional bias	102 – 145	44	Glu-rich
Sites
	Active site	271	1	Glycyl thioester intermediate By similarity
Amino acid modifications
	Modified residue	336	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q32L27 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 5BB0CFBD4222BA51	FASTA	342	38,717
	10 20 30 40 50 60 MSVSGLKAEL KFLASIFDKN HERFRIVSWK LDELHCQFLV PPPAPPGSPH SPPPPLTLHC 70 80 90 100 110 120 NITESYPSSS PIWFVDSDDP NLTSVLERLE DTKNNNSNGT TEEVTSEEEE EEEMAEDIED 130 140 150 160 170 180 LDHYEMKEEE PISGKKSEDE GIEKENLAIL EKIRKTQRQD HLNGAVSGSV QASDRLMKEL 190 200 210 220 230 240 RDIYRSQSYK TGIYSVELIN DSLYDWHVKL QKVDPDSPLH SDLQILKEKE GIEYILLNFS 250 260 270 280 290 300 FKDNFPFDPP FVRVVLPVLS GGYVLGGGAL CMELLTKQGW SSAYSIESVI MQINATLVKG 310 320 330 340 KARVQFGANK NQYNLARAQQ SYNSIVQIHE KNGWYTPPKE DG

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC109798 mRNA. Translation: AAI09799.1.
IPI	IPI00705499.
RefSeq	NP_001068998.1. NM_001075530.1.
UniGene	Bt.53371.
3D structure databases
ProteinModelPortal	Q32L27.
SMR	Q32L27. Positions 168-329.
ModBase	Search...
Proteomic databases
PRIDE	Q32L27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000005257; ENSBTAP00000005257; ENSBTAG00000004024.
GeneID	511631.
KEGG	bta:511631.
Organism-specific databases
CTD	92912.
Phylogenomic databases
eggNOG	maNOG07693.
GeneTree	ENSGT00390000018573.
HOVERGEN	HBG061368.
OrthoDB	EOG48D0VK.
Family and domain databases
InterPro	IPR000608. UBQ-conjugat_E2. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 2 hits.
KO	K10582.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 2 hits.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UB2Q2_BOVIN

Accession

Primary (citable) accession number: Q32L27

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	December 6, 2005
Last modified:	November 16, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program