Reviewed, UniProtKB/Swiss-Prot Q32L27 (UB2Q2_BOVIN)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 Q2
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase Q2
    Ubiquitin carrier protein Q2

Gene names

Name:

UBE2Q2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Ubiquitin-conjugating enzyme E2 Q2		PRO_0000223878
Regions
	Compositional bias	41 – 55	15	Pro-rich
	Compositional bias	102 – 145	44	Glu-rich
Sites
	Active site	271	1	Glycyl thioester intermediate By similarity
Amino acid modifications
	Modified residue	336	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q32L27-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 5BB0CFBD4222BA51	FASTA	342	38,717
	10 20 30 40 50 60 MSVSGLKAEL KFLASIFDKN HERFRIVSWK LDELHCQFLV PPPAPPGSPH SPPPPLTLHC 70 80 90 100 110 120 NITESYPSSS PIWFVDSDDP NLTSVLERLE DTKNNNSNGT TEEVTSEEEE EEEMAEDIED 130 140 150 160 170 180 LDHYEMKEEE PISGKKSEDE GIEKENLAIL EKIRKTQRQD HLNGAVSGSV QASDRLMKEL 190 200 210 220 230 240 RDIYRSQSYK TGIYSVELIN DSLYDWHVKL QKVDPDSPLH SDLQILKEKE GIEYILLNFS 250 260 270 280 290 300 FKDNFPFDPP FVRVVLPVLS GGYVLGGGAL CMELLTKQGW SSAYSIESVI MQINATLVKG 310 320 330 340 KARVQFGANK NQYNLARAQQ SYNSIVQIHE KNGWYTPPKE DG

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC109798 mRNA. Translation: AAI09799.1.
IPI	IPI00705499.
RefSeq	NP_001068998.1.
UniGene	Bt.53371
3D structure databases
SMR	Q32L27. Positions 168-329.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000005257; ENSBTAP00000005257; ENSBTAG00000004024; Bos taurus. [Genome view]
GeneID	511631.
KEGG	bta:511631.
Organism-specific databases
CTD	511631.
Phylogenomic databases
eggNOG	maNOG07693.
HOVERGEN	Q32L27.
OrthoDB	EOG9D81R5.
Enzyme and pathway databases
BRENDA	6.3.2.19. 251.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UB2Q2_BOVIN

Accession

Primary (citable) accession number: Q32L27

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	December 6, 2005
Last modified:	January 19, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)