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Protein

UBX domain-containing protein 1

Gene

UBXN1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Ubiquitin-binding protein that interacts with the BRCA1-BARD1 heterodimer, and regulates its activity. Specifically binds 'Lys-6'-linked polyubiquitin chains. Interaction with autoubiquitinated BRCA1, leads to inhibit the E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-BTA-532668. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
UBX domain-containing protein 1
Alternative name(s):
SAPK substrate protein 1
Gene namesi
Name:UBXN1
Synonyms:SAKS1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 29

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002489972 – 297UBX domain-containing protein 1Add BLAST296

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei199PhosphoserineBy similarity1
Modified residuei200Phosphoserine; by MAPK12By similarity1
Modified residuei207PhosphothreonineBy similarity1
Modified residuei229PhosphothreonineBy similarity1
Modified residuei270PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ32KW2.
PRIDEiQ32KW2.

Expressioni

Gene expression databases

BgeeiENSBTAG00000010481.

Interactioni

Subunit structurei

Component of a complex required to couple retrotranslocation, ubiquitination and deglycosylation composed of NGLY1, SAKS1, AMFR, VCP and RAD23B. Interacts with HOMER2 (By similarity). Interacts directly with VCP. Interacts with BRCA1 and BARD1; interaction takes place when BRCA1 is not autoubiquitinated bur is strongly enhanced in the presence of autoubiquitinated BRCA1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013845.

Structurei

3D structure databases

ProteinModelPortaliQ32KW2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 42UBAPROSITE-ProRule annotationAdd BLAST41
Domaini209 – 291UBXPROSITE-ProRule annotationAdd BLAST83

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni43 – 297Interaction with BRCA1By similarityAdd BLAST255

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili86 – 176Sequence analysisAdd BLAST91

Domaini

The UBA domain specifically recognizes and binds 'Lys-6'-linked polyubiquitin chains.By similarity

Sequence similaritiesi

Contains 1 UBA domain.PROSITE-ProRule annotation
Contains 1 UBX domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2689. Eukaryota.
ENOG4111HH6. LUCA.
GeneTreeiENSGT00530000063500.
HOGENOMiHOG000188321.
HOVERGENiHBG082310.
InParanoidiQ32KW2.
OMAiHTEVEYH.
OrthoDBiEOG091G0N23.
TreeFamiTF313944.

Family and domain databases

InterProiIPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
PF00789. UBX. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 1 hit.
SM00166. UBX. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
PS50033. UBX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q32KW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELTALESL IEMGFPKGRA EKALALTGNQ GIEAAMDWLM EHEDDPDVDE
60 70 80 90 100
PLATPLGHVL GREPTPSEQG GPKGPGSAVG EGKPVLTEEE RQEQTKRMLE
110 120 130 140 150
LVAQKQRERE EREEREALER ERQRRRQGQE LTAARQRLQE DEMRRAAEER
160 170 180 190 200
RREKAEELEA RQRVREKIER DKAERAKKYG GNVGSQPSPP ATEPGPVPSS
210 220 230 240 250
PSREPPTKRE YDQCRIQVRL PDGTSLTQTF RAREQLAAVR LYVELHRGEE
260 270 280 290
LGGAQDPVQL LSGFPRRAFS EADMERPLQE LGLVPSAVLI VAKKCPG
Length:297
Mass (Da):33,402
Last modified:December 6, 2005 - v1
Checksum:iEFC33173A0D803F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109898 mRNA. Translation: AAI09899.1.
RefSeqiNP_001032677.1. NM_001037588.2.
UniGeneiBt.8001.

Genome annotation databases

EnsembliENSBTAT00000013845; ENSBTAP00000013845; ENSBTAG00000010481.
GeneIDi506676.
KEGGibta:506676.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109898 mRNA. Translation: AAI09899.1.
RefSeqiNP_001032677.1. NM_001037588.2.
UniGeneiBt.8001.

3D structure databases

ProteinModelPortaliQ32KW2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013845.

Proteomic databases

PaxDbiQ32KW2.
PRIDEiQ32KW2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000013845; ENSBTAP00000013845; ENSBTAG00000010481.
GeneIDi506676.
KEGGibta:506676.

Organism-specific databases

CTDi51035.

Phylogenomic databases

eggNOGiKOG2689. Eukaryota.
ENOG4111HH6. LUCA.
GeneTreeiENSGT00530000063500.
HOGENOMiHOG000188321.
HOVERGENiHBG082310.
InParanoidiQ32KW2.
OMAiHTEVEYH.
OrthoDBiEOG091G0N23.
TreeFamiTF313944.

Enzyme and pathway databases

ReactomeiR-BTA-532668. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

Gene expression databases

BgeeiENSBTAG00000010481.

Family and domain databases

InterProiIPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
PF00789. UBX. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 1 hit.
SM00166. UBX. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
PS50033. UBX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBXN1_BOVIN
AccessioniPrimary (citable) accession number: Q32KW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: December 6, 2005
Last modified: October 5, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.