Reviewed, UniProtKB/Swiss-Prot Q32KR6 (FDFT_BOVIN)

Names and origin

Protein names

Recommended name:
    Squalene synthetase
      Short name=SQS
      Short name=SS
    EC=2.5.1.21
Alternative name(s):
    Farnesyl-diphosphate farnesyltransferase
    FPP:FPP farnesyltransferase

Gene names

Name:

FDFT1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Critical branch point enzyme of isoprenoid biosynthesis that is thought to regulate the flux of isoprene intermediates through the sterol pathway By similarity.
Catalytic activity	2 farnesyl diphosphate = diphosphate + presqualene diphosphate. Presqualene diphosphate + NAD(P)H = squalene + diphosphate + NAD(P)+.
Cofactor	Magnesium By similarity.
Pathway	Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the phytoene/squalene synthetase family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Isoprene biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	Magnesium NADP
Molecular function	Oxidoreductase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	farnesyl-diphosphate farnesyltransferase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Squalene synthetase		PRO_0000290005
Regions
	Transmembrane	284 – 304	21	Potential
	Transmembrane	384 – 404	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q32KR6-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: C6A2FD8EA5C85BC7	FASTA	417	48,304
	10 20 30 40 50 60 MEFVKCLGHP EEFYNLLRFQ MGGRRKVIPK MDQDSLSSSL KTCYKYLNQT SRSFAAVIQA 70 80 90 100 110 120 LDGEMRHAVC IFYLVLRALD TLEDDMTISI ERKVPLLHNF HSYLYEPDWR FTESKEKDRQ 130 140 150 160 170 180 VLEDFPTISL EFRNLAEKYQ TVIVDVCQKM GFGMAEFLDK RVTSEREWDK YCHYVAGLVG 190 200 210 220 230 240 IGLSRLFPAS ELEDPLIGED TERANSMGLF LQKTNIIRDY LEDQREGREF WPQETWSKYV 250 260 270 280 290 300 KKLGDFAKPE NIDLAVQCLN ELITNTLHHI PDVITYLSRL RNQSIFNFCA IPQVMAIATL 310 320 330 340 350 360 AACYNNQQVF KGVVKIRKGQ AVTLMMDATN MPAVKAIIHQ YMEEIYHRIP NSDPCSTKTQ 370 380 390 400 410 QIISTIRTQN LPNCQLVSRS HYSPIYLSFV MLLAALSWQY LSTLSQVTED YVQTGEH

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC109959 mRNA. Translation: AAI09960.1.
IPI	IPI00852555.
UniGene	Bt.14255
3D structure databases
SMR	Q32KR6. Positions 38-370.
ModBase	Search...
Protein-protein interaction databases
STRING	Q32KR6.
Genome annotation databases
Ensembl	ENSBTAT00000016504; ENSBTAP00000016504; ENSBTAG00000012432; Bos taurus. [Genome view]
Phylogenomic databases
HOVERGEN	Q32KR6.
Enzyme and pathway databases
BRENDA	2.5.1.21. 251.
Family and domain databases
InterPro	IPR002060. Squ/phyt_synthse. IPR006449. Squal_synth. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
TIGRFAMs	TIGR01559. squal_synth. 1 hit.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDFT_BOVIN

Accession

Primary (citable) accession number: Q32KR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	December 6, 2005
Last modified:	October 13, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)