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Protein

Squalene synthase

Gene

FDFT1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Critical branch point enzyme of isoprenoid biosynthesis that is thought to regulate the flux of isoprene intermediates through the sterol pathway.By similarity

Catalytic activityi

2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.

Cofactori

Mg2+By similarity

Pathwayi: lanosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Squalene synthase (FDFT1)
  2. no protein annotated in this organism
  3. Lanosterol synthase (LSS)
This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMultifunctional enzyme, Transferase
Biological processCholesterol biosynthesis, Cholesterol metabolism, Isoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandMagnesium, NADP

Enzyme and pathway databases

UniPathwayiUPA00767; UER00751

Names & Taxonomyi

Protein namesi
Recommended name:
Squalene synthase (EC:2.5.1.21)
Short name:
SQS
Short name:
SS
Alternative name(s):
FPP:FPP farnesyltransferase
Farnesyl-diphosphate farnesyltransferase
Gene namesi
Name:FDFT1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei284 – 304HelicalSequence analysisAdd BLAST21
Transmembranei384 – 404HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002900051 – 417Squalene synthaseAdd BLAST417

Proteomic databases

PaxDbiQ32KR6
PRIDEiQ32KR6

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000016504

Structurei

3D structure databases

ProteinModelPortaliQ32KR6
SMRiQ32KR6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1459 Eukaryota
COG1562 LUCA
HOGENOMiHOG000186940
HOVERGENiHBG002370
InParanoidiQ32KR6

Family and domain databases

CDDicd00683 Trans_IPPS_HH, 1 hit
Gene3Di1.10.600.10, 1 hit
InterProiView protein in InterPro
IPR008949 Isoprenoid_synthase_dom_sf
IPR002060 Squ/phyt_synthse
IPR006449 Squal_synth-like
IPR019845 Squalene/phytoene_synthase_CS
IPR033904 Trans_IPPS_HH
PfamiView protein in Pfam
PF00494 SQS_PSY, 1 hit
SUPFAMiSSF48576 SSF48576, 1 hit
TIGRFAMsiTIGR01559 squal_synth, 1 hit
PROSITEiView protein in PROSITE
PS01044 SQUALEN_PHYTOEN_SYN_1, 1 hit
PS01045 SQUALEN_PHYTOEN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q32KR6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFVKCLGHP EEFYNLLRFQ MGGRRKVIPK MDQDSLSSSL KTCYKYLNQT
60 70 80 90 100
SRSFAAVIQA LDGEMRHAVC IFYLVLRALD TLEDDMTISI ERKVPLLHNF
110 120 130 140 150
HSYLYEPDWR FTESKEKDRQ VLEDFPTISL EFRNLAEKYQ TVIVDVCQKM
160 170 180 190 200
GFGMAEFLDK RVTSEREWDK YCHYVAGLVG IGLSRLFPAS ELEDPLIGED
210 220 230 240 250
TERANSMGLF LQKTNIIRDY LEDQREGREF WPQETWSKYV KKLGDFAKPE
260 270 280 290 300
NIDLAVQCLN ELITNTLHHI PDVITYLSRL RNQSIFNFCA IPQVMAIATL
310 320 330 340 350
AACYNNQQVF KGVVKIRKGQ AVTLMMDATN MPAVKAIIHQ YMEEIYHRIP
360 370 380 390 400
NSDPCSTKTQ QIISTIRTQN LPNCQLVSRS HYSPIYLSFV MLLAALSWQY
410
LSTLSQVTED YVQTGEH
Length:417
Mass (Da):48,304
Last modified:December 6, 2005 - v1
Checksum:iC6A2FD8EA5C85BC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109959 mRNA Translation: AAI09960.1
UniGeneiBt.14255

Similar proteinsi

Entry informationi

Entry nameiFDFT_BOVIN
AccessioniPrimary (citable) accession number: Q32KR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: December 6, 2005
Last modified: April 25, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health