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Q32KL2 (PSB5_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit beta type-5

EC=3.4.25.1
Gene names
Name:PSMB5
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity By similarity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib By similarity. Plays a role in the protection against oxidative damage through the Nrf2-ARE pathway By similarity. UniProtKB P28074

Catalytic activity

Cleavage of peptide bonds with very broad specificity. UniProtKB P28074

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. This subunit can be displaced by the equivalent immune-specific subunit PSMB8. Directly interacts with POMP By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity UniProtKB P28074.

Sequence similarities

Belongs to the peptidase T1B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 5959Removed in mature form By similarity UniProtKB P28074
PRO_0000331491
Chain60 – 263204Proteasome subunit beta type-5 UniProtKB P28074
PRO_0000331492

Sites

Active site601Nucleophile By similarity
Binding site1081Bortezomib; via amide nitrogen By similarity

Secondary structure

................................... 263
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q32KL2 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 63FB47F28EE677BF

FASTA26328,609
        10         20         30         40         50         60 
MALASVLERP LSVNRRGFFG LGGRADLLDL GPGSPSDGLS LAAPSWGVPE EPRIEILHGT 

        70         80         90        100        110        120 
TTLAFKFRHG VIVAADSRAT AGAYIASQTV KKVIEINPYL LGTMAGGAAD CSFWERLLAR 

       130        140        150        160        170        180 
QCRIYELRNK ERISVAAASK LLANMVYQYK GMGLSMGTMI CGWDKRGPGL YYVDSEGNRI 

       190        200        210        220        230        240 
SGATFSVGSG SVYAYGVMDR GYSYDLEVEE AYDLARRAIY QATYRDAYSG GSVSLYHVRE 

       250        260 
DGWIRVSSDN VADLHDKYSG STH 

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC110039 mRNA. Translation: AAI10040.1.
RefSeqNP_001032701.1. NM_001037612.2.
UniGeneBt.5322.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IRUX-ray2.75L/Z60-262[»]
ProteinModelPortalQ32KL2.
SMRQ32KL2. Positions 60-260.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ32KL2. 1 interaction.
STRING9913.ENSBTAP00000016918.

Protein family/group databases

MEROPST01.P01.

Proteomic databases

PRIDEQ32KL2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000016918; ENSBTAP00000016918; ENSBTAG00000012726.
GeneID534640.
KEGGbta:534640.

Organism-specific databases

CTD5693.

Phylogenomic databases

eggNOGCOG0638.
GeneTreeENSGT00510000046395.
HOGENOMHOG000091082.
HOVERGENHBG108297.
InParanoidQ32KL2.
KOK02737.
OMALRAIMHA.
OrthoDBEOG7FNC86.
TreeFamTF106223.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSPR00141. PROTEASOME.
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ32KL2.
NextBio20876485.

Entry information

Entry namePSB5_BOVIN
AccessionPrimary (citable) accession number: Q32KL2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 6, 2005
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references