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Q32KL2

- PSB5_BOVIN

UniProt

Q32KL2 - PSB5_BOVIN

Protein

Proteasome subunit beta type-5

Gene

PSMB5

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity By similarity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib By similarity. Plays a role in the protection against oxidative damage through the Nrf2-ARE pathway By similarity.By similarity

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei60 – 601NucleophileBy similarity
    Binding sitei108 – 1081Bortezomib; via amide nitrogenBy similarity

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
    2. response to oxidative stress Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    ReactomeiREACT_203965. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_205339. APC/C:Cdc20 mediated degradation of Securin.
    REACT_205897. Activation of NF-kappaB in B cells.
    REACT_206368. CDT1 association with the CDC6:ORC:origin complex.
    REACT_207857. Asymmetric localization of PCP proteins.
    REACT_208116. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_208851. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_208889. degradation of AXIN.
    REACT_211738. ER-Phagosome pathway.
    REACT_212887. Separation of Sister Chromatids.
    REACT_213030. Orc1 removal from chromatin.
    REACT_215163. degradation of DVL.
    REACT_217860. SCF-beta-TrCP mediated degradation of Emi1.
    REACT_218058. Ubiquitin-dependent degradation of Cyclin D1.
    REACT_222557. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_222622. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    REACT_223494. SCF(Skp2)-mediated degradation of p27/p21.
    REACT_224434. Autodegradation of the E3 ubiquitin ligase COP1.
    REACT_225648. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_226120. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_227515. AUF1 (hnRNP D0) destabilizes mRNA.

    Protein family/group databases

    MEROPSiT01.P01.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit beta type-5 (EC:3.4.25.1)
    Gene namesi
    Name:PSMB5Imported
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 10

    Subcellular locationi

    Cytoplasm By similarityPROSITE-ProRule annotation. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. proteasome core complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 5959Removed in mature formBy similarityPRO_0000331491Add
    BLAST
    Chaini60 – 263204Proteasome subunit beta type-5By similarityPRO_0000331492Add
    BLAST

    Keywords - PTMi

    Zymogen

    Proteomic databases

    PRIDEiQ32KL2.

    Interactioni

    Subunit structurei

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. This subunit can be displaced by the equivalent immune-specific subunit PSMB8. Directly interacts with POMP By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ32KL2. 1 interaction.
    STRINGi9913.ENSBTAP00000016918.

    Structurei

    Secondary structure

    1
    263
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi62 – 676
    Beta strandi70 – 756
    Beta strandi79 – 813
    Beta strandi84 – 885
    Beta strandi93 – 975
    Beta strandi100 – 1034
    Helixi108 – 12922
    Helixi135 – 14713
    Turni148 – 1514
    Beta strandi156 – 1638
    Beta strandi165 – 17713
    Beta strandi179 – 1813
    Beta strandi183 – 1886
    Helixi191 – 1999
    Helixi208 – 22518
    Beta strandi231 – 2388
    Beta strandi243 – 2508
    Helixi251 – 2588

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1IRUX-ray2.75L/Z60-262[»]
    ProteinModelPortaliQ32KL2.
    SMRiQ32KL2. Positions 60-260.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ32KL2.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    GeneTreeiENSGT00510000046395.
    HOGENOMiHOG000091082.
    HOVERGENiHBG108297.
    InParanoidiQ32KL2.
    KOiK02737.
    OMAiLRAIMHA.
    OrthoDBiEOG7FNC86.
    TreeFamiTF106223.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR000243. Pept_T1A_subB.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    PRINTSiPR00141. PROTEASOME.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q32KL2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALASVLERP LSVNRRGFFG LGGRADLLDL GPGSPSDGLS LAAPSWGVPE    50
    EPRIEILHGT TTLAFKFRHG VIVAADSRAT AGAYIASQTV KKVIEINPYL 100
    LGTMAGGAAD CSFWERLLAR QCRIYELRNK ERISVAAASK LLANMVYQYK 150
    GMGLSMGTMI CGWDKRGPGL YYVDSEGNRI SGATFSVGSG SVYAYGVMDR 200
    GYSYDLEVEE AYDLARRAIY QATYRDAYSG GSVSLYHVRE DGWIRVSSDN 250
    VADLHDKYSG STH 263
    Length:263
    Mass (Da):28,609
    Last modified:December 6, 2005 - v1
    Checksum:i63FB47F28EE677BF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC110039 mRNA. Translation: AAI10040.1.
    RefSeqiNP_001032701.1. NM_001037612.2.
    UniGeneiBt.5322.

    Genome annotation databases

    EnsembliENSBTAT00000016918; ENSBTAP00000016918; ENSBTAG00000012726.
    GeneIDi534640.
    KEGGibta:534640.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC110039 mRNA. Translation: AAI10040.1 .
    RefSeqi NP_001032701.1. NM_001037612.2.
    UniGenei Bt.5322.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1IRU X-ray 2.75 L/Z 60-262 [» ]
    ProteinModelPortali Q32KL2.
    SMRi Q32KL2. Positions 60-260.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q32KL2. 1 interaction.
    STRINGi 9913.ENSBTAP00000016918.

    Protein family/group databases

    MEROPSi T01.P01.

    Proteomic databases

    PRIDEi Q32KL2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000016918 ; ENSBTAP00000016918 ; ENSBTAG00000012726 .
    GeneIDi 534640.
    KEGGi bta:534640.

    Organism-specific databases

    CTDi 5693.

    Phylogenomic databases

    eggNOGi COG0638.
    GeneTreei ENSGT00510000046395.
    HOGENOMi HOG000091082.
    HOVERGENi HBG108297.
    InParanoidi Q32KL2.
    KOi K02737.
    OMAi LRAIMHA.
    OrthoDBi EOG7FNC86.
    TreeFami TF106223.

    Enzyme and pathway databases

    Reactomei REACT_203965. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_205339. APC/C:Cdc20 mediated degradation of Securin.
    REACT_205897. Activation of NF-kappaB in B cells.
    REACT_206368. CDT1 association with the CDC6:ORC:origin complex.
    REACT_207857. Asymmetric localization of PCP proteins.
    REACT_208116. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_208851. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_208889. degradation of AXIN.
    REACT_211738. ER-Phagosome pathway.
    REACT_212887. Separation of Sister Chromatids.
    REACT_213030. Orc1 removal from chromatin.
    REACT_215163. degradation of DVL.
    REACT_217860. SCF-beta-TrCP mediated degradation of Emi1.
    REACT_218058. Ubiquitin-dependent degradation of Cyclin D1.
    REACT_222557. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_222622. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    REACT_223494. SCF(Skp2)-mediated degradation of p27/p21.
    REACT_224434. Autodegradation of the E3 ubiquitin ligase COP1.
    REACT_225648. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_226120. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_227515. AUF1 (hnRNP D0) destabilizes mRNA.

    Miscellaneous databases

    EvolutionaryTracei Q32KL2.
    NextBioi 20876485.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR000243. Pept_T1A_subB.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    PRINTSi PR00141. PROTEASOME.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X AngusImported.
      Tissue: LiverImported.

    Entry informationi

    Entry nameiPSB5_BOVIN
    AccessioniPrimary (citable) accession number: Q32KL2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3