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Protein

Arylsulfatase I

Gene

Arsi

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Displays arylsulfatase activity at neutral pH, when co-expressed with SUMF1; arylsulfatase activity is measured in the secretion medium of retinal cell line, but no activity is recorded when measured in cell extracts.By similarity

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi55CalciumBy similarity1
Metal bindingi56CalciumBy similarity1
Active sitei93NucleophileBy similarity1
Metal bindingi93Calcium; via 3-oxoalanineBy similarity1
Binding sitei147SubstrateBy similarity1
Active sitei149By similarity1
Binding sitei239SubstrateBy similarity1
Metal bindingi297CalciumBy similarity1
Metal bindingi298CalciumBy similarity1
Binding sitei315SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-1660662 Glycosphingolipid metabolism
R-RNO-1663150 The activation of arylsulfatases

Names & Taxonomyi

Protein namesi
Recommended name:
Arylsulfatase I (EC:3.1.6.-)
Short name:
ASI
Gene namesi
Name:Arsi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi1310242 Arsi

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000035628524 – 573Arylsulfatase IAdd BLAST550

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei933-oxoalanine (Cys)By similarity1
Glycosylationi276N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi288N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi466N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi496N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The oxidation of Cys-93 residue to 3-oxoalanine (also known as C(alpha)-formylglycine) by SUMF1/Sulfatase-modifying factor 1, seems critical for catalytic activity.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ32KJ8
PRIDEiQ32KJ8

PTM databases

iPTMnetiQ32KJ8
PhosphoSitePlusiQ32KJ8

Expressioni

Gene expression databases

BgeeiENSRNOG00000026060
GenevisibleiQ32KJ8 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000033542

Structurei

3D structure databases

ProteinModelPortaliQ32KJ8
SMRiQ32KJ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi527 – 537Poly-GluAdd BLAST11

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3867 Eukaryota
COG3119 LUCA
GeneTreeiENSGT00760000119062
HOGENOMiHOG000135354
HOVERGENiHBG004282
InParanoidiQ32KJ8
KOiK12375
OMAiRYRSMGN
OrthoDBiEOG091G06C3
PhylomeDBiQ32KJ8
TreeFamiTF314186

Family and domain databases

Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR017850 Alkaline_phosphatase_core_sf
IPR024607 Sulfatase_CS
IPR000917 Sulfatase_N
PfamiView protein in Pfam
PF00884 Sulfatase, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00523 SULFATASE_1, 1 hit
PS00149 SULFATASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q32KJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHALSGFSLV SLLSLGYLSW DWAKPGLVAD GPAEAEDQPS AAPPQPPHII
60 70 80 90 100
FILTDDQGYH DVGYHGSDIE TPTLDRLAAE GVKLENYYIQ PICTPSRSQL
110 120 130 140 150
LTGRYQIHTG LQHSIIRPRQ PNCLPLDQVT LPQKLQEAGY STHMVGKWHL
160 170 180 190 200
GFYRKECLPT RRGFDTFLGS LTGNVDYYTY DNCDGPGVCG FDLHEGESVA
210 220 230 240 250
WGLSGQYSTM LYAQRASHIL ASHSPQKPLF LYVAFQAVHT PLQSPREYLY
260 270 280 290 300
RYRTMGNVAR RKYAAMVTCM DEAVRNITWA LKRYGFYNNS VIIFSSDNGG
310 320 330 340 350
QTFSGGSNWP LRGRKGTYWE GGVRGLGFVH SPLLKKKRRT SRALVHITDW
360 370 380 390 400
YPTLVGLAGG TTSAADGLDG YDVWPAISEG RASPRTEILH NIDPLYNHAR
410 420 430 440 450
HGSLEGGFGI WNTAVQAAIR VGEWKLLTGD PGYGDWIPPQ TLASFPGSWW
460 470 480 490 500
NLERMASIRQ AVWLFNISAD PYEREDLADQ RPDVVRTLLA RLADYNRTAI
510 520 530 540 550
PVRYPAANPR AHPDFNGGAW GPWASDEDEE EEDEEEEGRA RSFPRGRRKK
560 570
KCKICKLRSF FRKLNTRLMS HRI
Length:573
Mass (Da):64,424
Last modified:December 6, 2005 - v1
Checksum:iEEC97A0D8A57D367
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03109797 Genomic DNA No translation available.
BN000739 mRNA Translation: CAI84985.1
RefSeqiNP_001041346.1, NM_001047881.1
UniGeneiRn.202490

Genome annotation databases

EnsembliENSRNOT00000030966; ENSRNOP00000033542; ENSRNOG00000026060
GeneIDi307404
KEGGirno:307404
UCSCiRGD:1310242 rat

Similar proteinsi

Entry informationi

Entry nameiARSI_RAT
AccessioniPrimary (citable) accession number: Q32KJ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: December 6, 2005
Last modified: May 23, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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