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Protein

Arylsulfatase I

Gene

Arsi

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Displays arylsulfatase activity at neutral pH, when co-expressed with SUMF1; arylsulfatase activity is measured in the secretion medium of retinal cell line, but no activity is recorded when measured in cell extracts.By similarity

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi55CalciumBy similarity1
Metal bindingi56CalciumBy similarity1
Metal bindingi93Calcium; via 3-oxoalanineBy similarity1
Binding sitei147SubstrateBy similarity1
Active sitei149By similarity1
Binding sitei239SubstrateBy similarity1
Metal bindingi297CalciumBy similarity1
Metal bindingi298CalciumBy similarity1
Binding sitei315SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.
R-MMU-1663150. The activation of arylsulfatases.

Names & Taxonomyi

Protein namesi
Recommended name:
Arylsulfatase I (EC:3.1.6.-)
Short name:
ASI
Gene namesi
Name:Arsi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2670959. Arsi.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000035628424 – 573Arylsulfatase IAdd BLAST550

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei933-oxoalanine (Cys)By similarity1
Glycosylationi276N-linked (GlcNAc...)Sequence analysis1
Glycosylationi288N-linked (GlcNAc...)Sequence analysis1
Glycosylationi466N-linked (GlcNAc...)Sequence analysis1
Glycosylationi496N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

The oxidation of Cys-93 residue to 3-oxoalanine (also known as C(alpha)-formylglycine) by SUMF1/Sulfatase-modifying factor 1, seems critical for catalytic activity.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ32KI9.
PRIDEiQ32KI9.

PTM databases

iPTMnetiQ32KI9.
PhosphoSitePlusiQ32KI9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000036412.
GenevisibleiQ32KI9. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043966.

Structurei

3D structure databases

ProteinModelPortaliQ32KI9.
SMRiQ32KI9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi526 – 537Poly-GluAdd BLAST12

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3867. Eukaryota.
COG3119. LUCA.
GeneTreeiENSGT00760000119062.
HOGENOMiHOG000135354.
HOVERGENiHBG004282.
InParanoidiQ32KI9.
KOiK12375.
OMAiPLYNHAK.
OrthoDBiEOG091G06C3.
PhylomeDBiQ32KI9.
TreeFamiTF314186.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q32KI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHALSGFSLV SLLSLGYLSW DWAKPGLVAD GPAEAGDQPS VAPPQPPHII
60 70 80 90 100
FILTDDQGYH DVGYHGSDIE TPTLDRLAAE GVKLENYYIQ PICTPSRSQL
110 120 130 140 150
LTGRYQIHTG LQHSIIRPRQ PNCLPLDQVT LPQKLQEAGY STHMVGKWHL
160 170 180 190 200
GFYRKECLPT RRGFDTFLGS LTGNVDYYTY DNCDGPGVCG FDLHEGESVA
210 220 230 240 250
WGLSGQYSTM LYAQRASHIL ASHNPQNPLF LYVAFQAVHT PLQSPREYLY
260 270 280 290 300
RYRTMGNVAR RKYAAMVTCM DEAVRNITWA LKRYGFYNNS VIIFSSDNGG
310 320 330 340 350
QTFSGGSNWP LRGRKGTYWE GGVRGLGFVH SPLLKKKRRT SRALVHITDW
360 370 380 390 400
YPTLVGLAGG TTSAADGLDG YDVWPAISEG RASPRTEILH NIDPLYNHAR
410 420 430 440 450
HGSLEGGFGI WNTAVQAAIR VGEWKLLTGD PGYGDWIPPQ TLASFPGSWW
460 470 480 490 500
NLERMASIRQ AVWLFNISAD PYEREDLAGQ RPDVVRTLLA RLADYNRTAI
510 520 530 540 550
PVRYPAANPR AHPDFNGGAW GPWASEEEEE EEEEEEEGRA RSFSRGRRKK
560 570
KCKICKLRSF FRKLNTRLMS HRI
Length:573
Mass (Da):64,367
Last modified:December 6, 2005 - v1
Checksum:iC12EF772465AA21E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH466528 Genomic DNA. Translation: EDL09797.1.
BC138970 mRNA. Translation: AAI38971.1.
BC141169 mRNA. Translation: AAI41170.1.
BN000748 mRNA. Translation: CAI84994.1.
CCDSiCCDS29275.1.
RefSeqiNP_001033588.1. NM_001038499.1.
UniGeneiMm.20147.

Genome annotation databases

EnsembliENSMUST00000040359; ENSMUSP00000043966; ENSMUSG00000036412.
GeneIDi545260.
KEGGimmu:545260.
UCSCiuc008fbe.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH466528 Genomic DNA. Translation: EDL09797.1.
BC138970 mRNA. Translation: AAI38971.1.
BC141169 mRNA. Translation: AAI41170.1.
BN000748 mRNA. Translation: CAI84994.1.
CCDSiCCDS29275.1.
RefSeqiNP_001033588.1. NM_001038499.1.
UniGeneiMm.20147.

3D structure databases

ProteinModelPortaliQ32KI9.
SMRiQ32KI9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043966.

PTM databases

iPTMnetiQ32KI9.
PhosphoSitePlusiQ32KI9.

Proteomic databases

PaxDbiQ32KI9.
PRIDEiQ32KI9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040359; ENSMUSP00000043966; ENSMUSG00000036412.
GeneIDi545260.
KEGGimmu:545260.
UCSCiuc008fbe.1. mouse.

Organism-specific databases

CTDi340075.
MGIiMGI:2670959. Arsi.

Phylogenomic databases

eggNOGiKOG3867. Eukaryota.
COG3119. LUCA.
GeneTreeiENSGT00760000119062.
HOGENOMiHOG000135354.
HOVERGENiHBG004282.
InParanoidiQ32KI9.
KOiK12375.
OMAiPLYNHAK.
OrthoDBiEOG091G06C3.
PhylomeDBiQ32KI9.
TreeFamiTF314186.

Enzyme and pathway databases

ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.
R-MMU-1663150. The activation of arylsulfatases.

Miscellaneous databases

PROiQ32KI9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036412.
GenevisibleiQ32KI9. MM.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARSI_MOUSE
AccessioniPrimary (citable) accession number: Q32KI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.