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Q32JI1

- THII_SHIDS

UniProt

Q32JI1 - THII_SHIDS

Protein

tRNA sulfurtransferase

Gene

thiI

Organism
Shigella dysenteriae serotype 1 (strain Sd197)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

    Catalytic activityi

    L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
    [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei265 – 2651ATPUniRule annotation
    Binding sitei287 – 2871ATP; via amide nitrogenUniRule annotation
    Binding sitei296 – 2961ATPUniRule annotation
    Active sitei456 – 4561Cysteine persulfide intermediateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi183 – 1842ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. sulfurtransferase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-HAMAP
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
    3. thiazole biosynthetic process Source: InterPro
    4. tRNA thio-modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Enzyme and pathway databases

    BioCyciSDYS300267:GJEW-306-MONOMER.
    UniPathwayiUPA00060.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
    Alternative name(s):
    Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
    Thiamine biosynthesis protein ThiIUniRule annotation
    tRNA 4-thiouridine synthaseUniRule annotation
    Gene namesi
    Name:thiIUniRule annotation
    Ordered Locus Names:SDY_0307
    OrganismiShigella dysenteriae serotype 1 (strain Sd197)
    Taxonomic identifieri300267 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
    ProteomesiUP000002716: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 482482tRNA sulfurtransferasePRO_1000074278Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi344 ↔ 456Redox-activeUniRule annotation

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi300267.SDY_0307.

    Structurei

    3D structure databases

    ProteinModelPortaliQ32JI1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini61 – 165105THUMPUniRule annotationAdd
    BLAST
    Domaini404 – 48279RhodaneseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ThiI family.UniRule annotation
    Contains 1 rhodanese domain.UniRule annotation
    Contains 1 THUMP domain.UniRule annotation

    Keywords - Domaini

    Redox-active center

    Phylogenomic databases

    eggNOGiCOG0301.
    HOGENOMiHOG000227469.
    KOiK03151.
    OMAiKLFPEIM.
    OrthoDBiEOG6TBHGR.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPiMF_00021. ThiI.
    InterProiIPR001763. Rhodanese-like_dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR026340. Thiazole_biosynth_dom.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view]
    PfamiPF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view]
    SMARTiSM00981. THUMP. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    TIGRFAMsiTIGR04271. ThiI_C_thiazole. 1 hit.
    TIGR00342. TIGR00342. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    PS51165. THUMP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q32JI1-1 [UniParc]FASTAAdd to Basket

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    MKFIIKLFPE ITIKSQSVRL RFIKILTGNI RNVLKHYDET LAVVRHWDNI    50
    EVRAKDENQR LTIRDALTRI PGIHHILEVE DVPFTDMHDI FEKALVQYRD 100
    QLEGKTFCVR VKRSGKHDFS SIDVERYVGG GLNQHIESAR VKLTNPDVTV 150
    HLEVEDDRLL LIKGRYEGIG GFPIGTQEDV LSLISGGFDS GVSSYMLMRR 200
    GCRVHYCFFN LGGAAHEIGV RQVAHYLWNR FGSSHRVRFV AINFEPVVGE 250
    ILEKIDDGQM GVILKRMMVR AASKVAERYG VQALVTGEAL GQVSSQTLTN 300
    LRLIDNVSDT LILRPLISYD KEHIINLARQ IGTEDFARTM PEYCGVISKS 350
    PTVKAVKSKI EAEEEKFDFS ILDKVVEEAN NVDIREIAQQ TEQEVVEVET 400
    VNDCGPNDVI LDIRSVDEQE DKPLKVEGID VVSLPFYKLS TKFGDLDQNR 450
    TWLLWCERGV MSRLQALYLR EQGFNNVKVY RP 482
    Length:482
    Mass (Da):54,962
    Last modified:December 6, 2005 - v1
    Checksum:i00FAB35849600966
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000034 Genomic DNA. Translation: ABB60526.1.
    RefSeqiYP_402015.1. NC_007606.1.

    Genome annotation databases

    EnsemblBacteriaiABB60526; ABB60526; SDY_0307.
    GeneIDi3799691.
    KEGGisdy:SDY_0307.
    PATRICi18689724. VBIShiDys99784_0352.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000034 Genomic DNA. Translation: ABB60526.1 .
    RefSeqi YP_402015.1. NC_007606.1.

    3D structure databases

    ProteinModelPortali Q32JI1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 300267.SDY_0307.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABB60526 ; ABB60526 ; SDY_0307 .
    GeneIDi 3799691.
    KEGGi sdy:SDY_0307.
    PATRICi 18689724. VBIShiDys99784_0352.

    Phylogenomic databases

    eggNOGi COG0301.
    HOGENOMi HOG000227469.
    KOi K03151.
    OMAi KLFPEIM.
    OrthoDBi EOG6TBHGR.

    Enzyme and pathway databases

    UniPathwayi UPA00060 .
    BioCyci SDYS300267:GJEW-306-MONOMER.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPi MF_00021. ThiI.
    InterProi IPR001763. Rhodanese-like_dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR026340. Thiazole_biosynth_dom.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view ]
    Pfami PF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view ]
    SMARTi SM00981. THUMP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    TIGRFAMsi TIGR04271. ThiI_C_thiazole. 1 hit.
    TIGR00342. TIGR00342. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    PS51165. THUMP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sd197.

    Entry informationi

    Entry nameiTHII_SHIDS
    AccessioniPrimary (citable) accession number: Q32JI1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3