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Protein

tRNA sulfurtransferase

Gene

thiI

Organism
Shigella dysenteriae serotype 1 (strain Sd197)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

Catalytic activityi

ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.UniRule annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei265ATPUniRule annotation1
Binding sitei287ATP; via amide nitrogenUniRule annotation1
Binding sitei296ATPUniRule annotation1
Active sitei456Cysteine persulfide intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi183 – 184ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase, tRNA-binding
Biological processThiamine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSDYS300267:G1G4A-329-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
Ordered Locus Names:SDY_0307
OrganismiShigella dysenteriae serotype 1 (strain Sd197)
Taxonomic identifieri300267 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002716 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000742781 – 482tRNA sulfurtransferaseAdd BLAST482

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi344 ↔ 456Redox-activeUniRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ32JI1

Structurei

3D structure databases

ProteinModelPortaliQ32JI1
SMRiQ32JI1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 165THUMPUniRule annotationAdd BLAST105
Domaini404 – 482RhodaneseUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

HOGENOMiHOG000227469
KOiK03151
OMAiKLFPEIM
OrthoDBiPOG091H04VG

Family and domain databases

CDDicd01712 ThiI, 1 hit
Gene3Di3.40.250.10, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00021 ThiI, 1 hit
InterProiView protein in InterPro
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
IPR014729 Rossmann-like_a/b/a_fold
IPR026340 Thiazole_biosynth_dom
IPR020536 ThiI_AANH
IPR004114 THUMP_dom
IPR003720 tRNA_STrfase
PfamiView protein in Pfam
PF02568 ThiI, 1 hit
PF02926 THUMP, 1 hit
SMARTiView protein in SMART
SM00981 THUMP, 1 hit
SUPFAMiSSF52821 SSF52821, 1 hit
TIGRFAMsiTIGR04271 ThiI_C_thiazole, 1 hit
TIGR00342 TIGR00342, 1 hit
PROSITEiView protein in PROSITE
PS50206 RHODANESE_3, 1 hit
PS51165 THUMP, 1 hit

Sequencei

Sequence statusi: Complete.

Q32JI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFIIKLFPE ITIKSQSVRL RFIKILTGNI RNVLKHYDET LAVVRHWDNI
60 70 80 90 100
EVRAKDENQR LTIRDALTRI PGIHHILEVE DVPFTDMHDI FEKALVQYRD
110 120 130 140 150
QLEGKTFCVR VKRSGKHDFS SIDVERYVGG GLNQHIESAR VKLTNPDVTV
160 170 180 190 200
HLEVEDDRLL LIKGRYEGIG GFPIGTQEDV LSLISGGFDS GVSSYMLMRR
210 220 230 240 250
GCRVHYCFFN LGGAAHEIGV RQVAHYLWNR FGSSHRVRFV AINFEPVVGE
260 270 280 290 300
ILEKIDDGQM GVILKRMMVR AASKVAERYG VQALVTGEAL GQVSSQTLTN
310 320 330 340 350
LRLIDNVSDT LILRPLISYD KEHIINLARQ IGTEDFARTM PEYCGVISKS
360 370 380 390 400
PTVKAVKSKI EAEEEKFDFS ILDKVVEEAN NVDIREIAQQ TEQEVVEVET
410 420 430 440 450
VNDCGPNDVI LDIRSVDEQE DKPLKVEGID VVSLPFYKLS TKFGDLDQNR
460 470 480
TWLLWCERGV MSRLQALYLR EQGFNNVKVY RP
Length:482
Mass (Da):54,962
Last modified:December 6, 2005 - v1
Checksum:i00FAB35849600966
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000034 Genomic DNA Translation: ABB60526.1
RefSeqiWP_000668706.1, NC_007606.1
YP_402015.1, NC_007606.1

Genome annotation databases

EnsemblBacteriaiABB60526; ABB60526; SDY_0307
GeneIDi3799691
KEGGisdy:SDY_0307
PATRICifig|300267.13.peg.352

Similar proteinsi

Entry informationi

Entry nameiTHII_SHIDS
AccessioniPrimary (citable) accession number: Q32JI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 6, 2005
Last modified: February 28, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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