Reviewed, UniProtKB/Swiss-Prot Q32HN5 (GHRA_SHIDS)

Names and origin

Protein names

Recommended name:
    Glyoxylate/hydroxypyruvate reductase A
    EC=1.1.1.79
    EC=1.1.1.81
Alternative name(s):
    2-ketoacid reductase

Gene names

Name:	ghrA
Ordered Locus Names:	SDY_1004

Organism

Shigella dysenteriae serotype 1 (strain Sd197) [Complete proteome] [HAMAP]

Taxonomic identifier

300267 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity. HAMAP MF_01666
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01666 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01666
Subcellular location	Cytoplasm Probable HAMAP MF_01666.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	cofactor binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: HAMAP hydroxypyruvate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Glyoxylate/hydroxypyruvate reductase A HAMAP MF_01666		PRO_0000348376
Sites
	Active site	227	1	By similarity
	Active site	275	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q32HN5-1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: 6A56639BBB816E42	FASTA	312	35,445
	10 20 30 40 50 60 MDIIFYHPTF DTQWWIKALR KAIPQARVRA WKSGDNDSAD YALVWHPPVE MLAGRDLKAV 70 80 90 100 110 120 FALGAGVDSI LSKLQAHPEM LNPYVPLFRL EDTGMGEQMQ EYAVSQVLHW FRRFDDYRIQ 130 140 150 160 170 180 QNSSHWQPLP EYYREDFTIG ILGAGVLGSK VAQSLQTWRF PLRCWSRTRK SWPGVQSFAG 190 200 210 220 230 240 REELSAFLSQ CRVLINLLPN TPETVGIINQ QLLEKLPDGA YLLNLARGVH VVEDDLLAAL 250 260 270 280 290 300 DSGKVKGAML DVFNREPLPP ESPLWQHPRV TITPHVAAIT RPAEAVEYIS RTIAQLEKGE 310 RVCGQVDRAR GY

References

[1]

"Genome dynamics and diversity of Shigella species, the etiologic agents of bacillary dysentery." Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X., Wang J., Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S., Nie H., Peng J., Xu J. , Wang Y., Yuan Z., Wen Y., Yao Z., Shen Y., Qiang B., Hou Y., Yu J., Jin Q. Nucleic Acids Res. 33:6445-6458(2005) [PubMed: 16275786] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000034 Genomic DNA. Translation: ABB61170.1. Different initiation.
RefSeq	YP_402661.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q32HN5.
Genome annotation databases
GeneID	3797139.
GenomeReviews	Gene locus SDY_1004 in contig CP000034_GR.
KEGG	sdy:SDY_1004.
NMPDR	fig|216598.1.peg.1027.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0111.
HOGENOM	HBG731446.
Enzyme and pathway databases
BioCyc	SDYS300267:SDY_1004-MONOMER.
Family and domain databases
HAMAP	MF_01666. 2-Hacid_dh_C_GhrA. [Tree]
InterPro	IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRA_SHIDS

Accession

Primary (citable) accession number: Q32HN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	January 19, 2010
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)