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Q32GM1

- STXA_SHIDS

UniProt

Q32GM1 - STXA_SHIDS

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Protein
Shiga toxin subunit A
Gene
stxA, SDY_1389
Organism
Shigella dysenteriae serotype 1 (strain Sd197)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subunit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits By similarity.

Catalytic activityi

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei189 – 1891 By similarity
Sitei273 – 2742Cleavage; by furin By similarity

GO - Molecular functioni

  1. rRNA N-glycosylase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. negative regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein synthesis inhibitor, Toxin

Enzyme and pathway databases

BioCyciSDYS300267:GJEW-1385-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Shiga toxin subunit A (EC:3.2.2.22)
Gene namesi
Name:stxA
Ordered Locus Names:SDY_1389
OrganismiShigella dysenteriae serotype 1 (strain Sd197)
Taxonomic identifieri300267 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000002716: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed prediction
Add
BLAST
Chaini23 – 315293Shiga toxin subunit A
PRO_0000312303Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi264 ↔ 283 By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Shiga toxin contains a single subunit A and five copies of subunit B By similarity.

Protein-protein interaction databases

STRINGi300267.SDY_1389.

Structurei

3D structure databases

ProteinModelPortaliQ32GM1.
SMRiQ32GM1. Positions 23-312.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 273251A1
Add
BLAST
Regioni274 – 31542A2
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000010102.
KOiK11006.
OrthoDBiEOG6BGNXJ.

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR016331. Shiga-like_toxin_subunit_A.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PIRSFiPIRSF001924. Shigella_toxin_subunit_A. 1 hit.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q32GM1-1 [UniParc]FASTAAdd to Basket

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MKIIIFRVLT FFFVIFSVNV VAKEFTLDFS TAKTYVDSLN VIRSAIGTPL    50
QTISSGGTSL LMIDSGTGDN LFAVDVRGID PEEGRFNNLR LIVERNNLYV 100
TGFVNRTNNV FYRFADFSHV TFPGTTAVTL SGDSSYTTLQ RVAGISRTGM 150
QINRHSLTTS YLDLMSHSGT SLTQSVARAM LRFVTVTAEA LRFRQIQRGF 200
RTTLDDLSGR SYVMTAEDVD LTLNWGRLSS VLPDYHGQDS VRVGRISFGS 250
INAILGSVAL ILNCHHHASR VARMASDEFP SMCPADGRVR GITHNKILWD 300
SSTLGAILMR RTISS 315
Length:315
Mass (Da):34,814
Last modified:December 6, 2005 - v1
Checksum:i8A423DF7ABF58F30
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000034 Genomic DNA. Translation: ABB61534.1.
RefSeqiYP_403025.1. NC_007606.1.

Genome annotation databases

EnsemblBacteriaiABB61534; ABB61534; SDY_1389.
GeneIDi3796555.
KEGGisdy:SDY_1389.
PATRICi18692394. VBIShiDys99784_1650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000034 Genomic DNA. Translation: ABB61534.1 .
RefSeqi YP_403025.1. NC_007606.1.

3D structure databases

ProteinModelPortali Q32GM1.
SMRi Q32GM1. Positions 23-312.
ModBasei Search...

Protein-protein interaction databases

STRINGi 300267.SDY_1389.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB61534 ; ABB61534 ; SDY_1389 .
GeneIDi 3796555.
KEGGi sdy:SDY_1389.
PATRICi 18692394. VBIShiDys99784_1650.

Phylogenomic databases

HOGENOMi HOG000010102.
KOi K11006.
OrthoDBi EOG6BGNXJ.

Enzyme and pathway databases

BioCyci SDYS300267:GJEW-1385-MONOMER.

Family and domain databases

Gene3Di 3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProi IPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR016331. Shiga-like_toxin_subunit_A.
[Graphical view ]
Pfami PF00161. RIP. 1 hit.
[Graphical view ]
PIRSFi PIRSF001924. Shigella_toxin_subunit_A. 1 hit.
SUPFAMi SSF56371. SSF56371. 1 hit.
PROSITEi PS00275. SHIGA_RICIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sd197.

Entry informationi

Entry nameiSTXA_SHIDS
AccessioniPrimary (citable) accession number: Q32GM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 6, 2005
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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