Reviewed, UniProtKB/Swiss-Prot Q32G88 (ASTD_SHIDS)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	SDY_1531

Organism

Shigella dysenteriae serotype 1 (strain Sd197) [Complete proteome] [HAMAP]

Taxonomic identifier

300267 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. HAMAP MF_01174
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.
Sequence caution	The sequence ABB61667.1 differs from that shown. Reason: Erroneous termination at position 334. Translated as Trp.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174		PRO_0000262428
Regions
	Nucleotide binding	220 – 225	6	NAD By similarity
Sites
	Active site	243	1	By similarity
	Active site	277	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q32G88-1 [UniParc]. Last modified November 28, 2006. Version 2. Checksum: 5DBDA3D31D6AC1F9	FASTA	490	52,933
	10 20 30 40 50 60 MTLWINGDWV TGQGALRVKR NPVSGEVLWQ GNDADAAQVE QACRAARAAY PRWARLSFGD 70 80 90 100 110 120 RQVRVERFAG LLESNKAELT AIIARETGKP RWEAATEVTA MINKIAISIK AYHVRTGEQR 130 140 150 160 170 180 SEMPDGAASL RHRPHGVLAV FGPYNFPGHL PNGHIVPALL AGNTIIFKPS ELTPWSGEAV 190 200 210 220 230 240 MRLWQQAGLP PGVLNLVQGG RETGQALSAL EDLDGLLFTG SANTGYQLHR QLSGQPEKIL 250 260 270 280 290 300 ALEMGGNNPL IIDEVADIDA AVHLTIQSAF VTAGQRCTCA RRLLLKSGAQ GDAFLARLVA 310 320 330 340 350 360 VSQRLTPGNW DDEPQPFIGG LISEQAAQQV VTAWQQLEAM GGRTLLAPRL FQAETSLLTP 370 380 390 400 410 420 GIIEMTGVAG VPDEEVFGPL LRVWRCDTFD EAIRMANNTR FGLSCGLVSS EREKFDQLLL 430 440 450 460 470 480 EARAGIVNWK KPLTGAASTA PFGGIGASGN HRPSAWYAAD YCAWPMASLE SDSLTLLAML 490 NPGLDFSDEV

References

[1]

"Genome dynamics and diversity of Shigella species, the etiologic agents of bacillary dysentery." Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X., Wang J., Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S., Nie H., Peng J., Xu J. , Wang Y., Yuan Z., Wen Y., Yao Z., Shen Y., Qiang B., Hou Y., Yu J., Jin Q. Nucleic Acids Res. 33:6445-6458(2005) [PubMed: 16275786] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000034 Genomic DNA. Translation: ABB61667.1. Sequence problems.
RefSeq	YP_403158.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3799020.
GenomeReviews	Gene locus SDY_1531 in contig CP000034_GR.
KEGG	sdy:SDY_1531.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q32G88.
Enzyme and pathway databases
BioCyc	SDYS300267:SDY_1531-MON.
Family and domain databases
HAMAP	MF_01174. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR015590. Aldehyde_DH. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03240. arg_catab_astD. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_SHIDS

Accession

Primary (citable) accession number: Q32G88

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 28, 2006
Last modified:	June 16, 2009
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)