Q32CI7 (PIMT_SHIDS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-L-isoaspartate O-methyltransferase
EC=2.1.1.77

Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Short name=PIMT

Gene names

Name:	pcm
Ordered Locus Names:	SDY_2942

Organism

Shigella dysenteriae serotype 1 (strain Sd197)

Taxonomic identifier

300267 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity. HAMAP MF_00090
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester. HAMAP MF_00090
Subcellular location	Cytoplasm By similarity HAMAP MF_00090.
Sequence similarities	Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Protein-L-isoaspartate O-methyltransferase HAMAP MF_00090		PRO_1000004826
Sites
	Active site	59	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q32CI7 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: BFA179567527A118	FASTA	208	23,258
	10 20 30 40 50 60 MVSRRVQALL DQLRAQGIQD EQVLNALAAV PREKFVDEAF EQKAWDNIAL PIGQGQTISQ 70 80 90 100 110 120 PYMVARMTEL LELTPQSRVL EIGTGSGYQT AILAHLVQHV CSVERIKGLQ WQARRRLKNL 130 140 150 160 170 180 DLHNVSTRHG DGWQGWQARA PFDAIIVTAA PPEIPTALMT QLDEGGILVL PVGEEHQYLK 190 200 RVRRRGGEFI IDTVEAVRFV PLVKGELA

References

[1]

"Genome dynamics and diversity of Shigella species, the etiologic agents of bacillary dysentery." Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X., Wang J., Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S., Nie H., Peng J., Xu J. , Wang Y., Yuan Z., Wen Y., Yao Z., Shen Y., Qiang B., Hou Y., Yu J., Jin Q. Nucleic Acids Res. 33:6445-6458(2005) [PubMed: 16275786] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sd197.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000034 Genomic DNA. Translation: ABB62968.1.
RefSeq	YP_404459.1. NC_007606.1.
3D structure databases
ProteinModelPortal	Q32CI7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q32CI7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000084508; EBESCP00000081488; EBESCG00000083554.
GeneID	3796516.
GenomeReviews	Gene locus SDY_2942 in contig CP000034_GR.
KEGG	sdy:SDY_2942.
PATRIC	18696253. VBIShiDys99784_3534.
Phylogenomic databases
eggNOG	COG2518.
GeneTree	EBGT00050000011550.
HOGENOM	HBG699907.
OMA	YMVARMS.
PhylomeDB	Q32CI7.
ProtClustDB	PRK00312.
Enzyme and pathway databases
BioCyc	SDYS300267:SDY_2942-MONOMER.
Family and domain databases
HAMAP	MF_00090. PIMT. [Tree]
InterPro	IPR000682. PCMT. [Graphical view]
KO	K00573.
PANTHER	PTHR11579. PCMT. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. Pimt. 1 hit.
PROSITE	PS01279. PCMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIMT_SHIDS

Accession

Primary (citable) accession number: Q32CI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 6, 2005
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program