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Protein

Isocitrate dehydrogenase kinase/phosphatase

Gene

aceK

Organism
Shigella dysenteriae serotype 1 (strain Sd197)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation.UniRule annotation

Catalytic activityi

ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei336 – 3361ATPUniRule annotation
Active sitei371 – 3711UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi315 – 3217ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Protein phosphatase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSDYS300267:GJEW-4323-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase kinase/phosphataseUniRule annotation (EC:2.7.11.5UniRule annotation, EC:3.1.3.-UniRule annotation)
Short name:
IDH kinase/phosphataseUniRule annotation
Short name:
IDHK/PUniRule annotation
Gene namesi
Name:aceKUniRule annotation
Ordered Locus Names:SDY_4327
OrganismiShigella dysenteriae serotype 1 (strain Sd197)
Taxonomic identifieri300267 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
Proteomesi
  • UP000002716 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 578578Isocitrate dehydrogenase kinase/phosphatasePRO_0000259158Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the AceK family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000247673.
KOiK00906.
OMAiEPWYSVG.
OrthoDBiEOG6HQSJX.

Family and domain databases

HAMAPiMF_00747. AceK.
InterProiIPR010452. Isocitrate_DH_AceK.
[Graphical view]
PfamiPF06315. AceK. 1 hit.
[Graphical view]
PIRSFiPIRSF000719. AceK. 1 hit.
ProDomiPD043552. Isocitrate_DH_AceK. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Q328N5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRGLELLIA QTILQGFDAQ YGRFLEVTSG AQQRFEQADW HAVQQAMKNR
60 70 80 90 100
IHLYDHHVGL VVEQLRCITN GQSTDAEFLL RVKEHYTRLL PDYPRFEIAE
110 120 130 140 150
SFFNSVYCRL FDHRSLTPER LFIFSSQPER RFRTIPRPLA KDFYPEHGWE
160 170 180 190 200
ALLMRVLSDL PLRLPWQNKS RDINYIIRHL TETLGPENLS ESHLQVANEL
210 220 230 240 250
FYRNKAAWLV GKLITPSGTL PFLLPIHQTD DGELFIDTCL TTTAEASIVF
260 270 280 290 300
GFARSYFMVY APLPAALVEW LREILPGKTT AELYMAIGCQ KHAKTESYRE
310 320 330 340 350
YLVYLQGCNE QFIEAPGIRG MVMLVFTLPG FDRVFKVIKD RFAPQKEMSA
360 370 380 390 400
AHVRACYQLV KEHDRVGRMA DTQEFENFVL EKRHISPALM ELLLQEAAEK
410 420 430 440 450
ITDLGEQIVI RHLYIERRMV PLNIWLEQVE GQQLRDAIEE YGNAIRQLAA
460 470 480 490 500
ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC YMTEVNFRDI PPPRYPEDEL
510 520 530 540 550
ASEPWYSVSP GDVFPEEFRH WLCADPRIGP LFEEMHADLF RTDYWRALQN
560 570
RIREGHVEDV YAYRRRQRFS VLYGEMLF
Length:578
Mass (Da):67,712
Last modified:December 6, 2005 - v1
Checksum:i31C735F00FFEB927
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000034 Genomic DNA. Translation: ABB64220.1.
RefSeqiWP_001137251.1. NC_007606.1.
YP_405711.1. NC_007606.1.

Genome annotation databases

EnsemblBacteriaiABB64220; ABB64220; SDY_4327.
GeneIDi3796500.
KEGGisdy:SDY_4327.
PATRICi18699516. VBIShiDys99784_5107.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000034 Genomic DNA. Translation: ABB64220.1.
RefSeqiWP_001137251.1. NC_007606.1.
YP_405711.1. NC_007606.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB64220; ABB64220; SDY_4327.
GeneIDi3796500.
KEGGisdy:SDY_4327.
PATRICi18699516. VBIShiDys99784_5107.

Phylogenomic databases

HOGENOMiHOG000247673.
KOiK00906.
OMAiEPWYSVG.
OrthoDBiEOG6HQSJX.

Enzyme and pathway databases

BioCyciSDYS300267:GJEW-4323-MONOMER.

Family and domain databases

HAMAPiMF_00747. AceK.
InterProiIPR010452. Isocitrate_DH_AceK.
[Graphical view]
PfamiPF06315. AceK. 1 hit.
[Graphical view]
PIRSFiPIRSF000719. AceK. 1 hit.
ProDomiPD043552. Isocitrate_DH_AceK. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sd197.

Entry informationi

Entry nameiACEK_SHIDS
AccessioniPrimary (citable) accession number: Q328N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: December 6, 2005
Last modified: November 11, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.