ID   Q32862_9ASTR            Unreviewed;       744 AA.
AC   Q32862;
DT   01-NOV-1996, integrated into UniProtKB/TrEMBL.
DT   01-NOV-1996, sequence version 1.
DT   27-MAR-2024, entry version 106.
DE   RecName: Full=NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic {ECO:0000256|ARBA:ARBA00018648, ECO:0000256|RuleBase:RU364062};
DE            EC=7.1.1.- {ECO:0000256|RuleBase:RU364062};
DE   AltName: Full=NADH-plastoquinone oxidoreductase subunit 5 {ECO:0000256|RuleBase:RU364062};
GN   Name=ndhF {ECO:0000256|RuleBase:RU364062,
GN   ECO:0000313|EMBL:AAA81357.1};
OS   Perezia microcephala.
OG   Plastid; Chloroplast {ECO:0000313|EMBL:AAA81357.1}.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Mutisioideae; Nassauvieae;
OC   Perezia.
OX   NCBI_TaxID=40565 {ECO:0000313|EMBL:AAA81357.1};
RN   [1] {ECO:0000313|EMBL:AAA81357.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Kim K.-J., Jansen R.K.;
RT   "ndhF sequence evolution and phylogenetic relationships in the sunflower
RT   family.";
RL   Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN
CC       and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain
CC       and possibly in a chloroplast respiratory chain. The immediate electron
CC       acceptor for the enzyme in this species is believed to be
CC       plastoquinone. Couples the redox reaction to proton translocation, and
CC       thus conserves the redox energy in a proton gradient.
CC       {ECO:0000256|ARBA:ARBA00004059, ECO:0000256|RuleBase:RU364062}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a plastoquinone + (n+1) H(+)(in) + NADH = a plastoquinol + n
CC         H(+)(out) + NAD(+); Xref=Rhea:RHEA:42608, Rhea:RHEA-COMP:9561,
CC         Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62192;
CC         Evidence={ECO:0000256|ARBA:ARBA00001230,
CC         ECO:0000256|RuleBase:RU364062};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a plastoquinone + (n+1) H(+)(in) + NADPH = a plastoquinol + n
CC         H(+)(out) + NADP(+); Xref=Rhea:RHEA:42612, Rhea:RHEA-COMP:9561,
CC         Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:62192;
CC         Evidence={ECO:0000256|ARBA:ARBA00001558,
CC         ECO:0000256|RuleBase:RU364062};
CC   -!- SUBUNIT: NDH is composed of at least 16 different subunits, 5 of which
CC       are encoded in the nucleus. {ECO:0000256|ARBA:ARBA00011199,
CC       ECO:0000256|RuleBase:RU364062}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. Plastid,
CC       chloroplast thylakoid membrane {ECO:0000256|ARBA:ARBA00004454,
CC       ECO:0000256|RuleBase:RU364062}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004454, ECO:0000256|RuleBase:RU364062}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 5 family.
CC       {ECO:0000256|ARBA:ARBA00008200, ECO:0000256|RuleBase:RU364062}.
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DR   EMBL; L39407; AAA81357.1; -; Genomic_DNA.
DR   PIR; T13585; T13585.
DR   AlphaFoldDB; Q32862; -.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR   GO; GO:0048038; F:quinone binding; IEA:UniProtKB-KW.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   Gene3D; 1.20.5.2700; -; 1.
DR   InterPro; IPR002128; NADH_UbQ_OxRdtase_chlpt_su5_C.
DR   InterPro; IPR018393; NADHpl_OxRdtase_5_subgr.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   InterPro; IPR003945; NU5C-like.
DR   InterPro; IPR001516; Proton_antipo_N.
DR   NCBIfam; TIGR01974; NDH_I_L; 1.
DR   PANTHER; PTHR42829; NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5; 1.
DR   PANTHER; PTHR42829:SF2; NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5; 1.
DR   Pfam; PF01010; Proton_antipo_C; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   Pfam; PF00662; Proton_antipo_N; 1.
DR   PRINTS; PR01434; NADHDHGNASE5.
DR   PRINTS; PR01435; NPOXDRDTASE5.
PE   3: Inferred from homology;
KW   Chloroplast {ECO:0000256|ARBA:ARBA00022528, ECO:0000256|RuleBase:RU364062};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU364062};
KW   NAD {ECO:0000256|ARBA:ARBA00023027, ECO:0000256|RuleBase:RU364062};
KW   NADP {ECO:0000256|ARBA:ARBA00022857, ECO:0000256|RuleBase:RU364062};
KW   Plastid {ECO:0000256|RuleBase:RU364062, ECO:0000313|EMBL:AAA81357.1};
KW   Plastoquinone {ECO:0000256|ARBA:ARBA00022957,
KW   ECO:0000256|RuleBase:RU364062};
KW   Quinone {ECO:0000256|ARBA:ARBA00022719, ECO:0000256|RuleBase:RU364062};
KW   Thylakoid {ECO:0000256|ARBA:ARBA00023078, ECO:0000256|RuleBase:RU364062};
KW   Translocase {ECO:0000256|ARBA:ARBA00022967};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW   ECO:0000256|RuleBase:RU364062};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|RuleBase:RU364062}; Transport {ECO:0000256|RuleBase:RU364062}.
FT   TRANSMEM        6..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU364062"
FT   TRANSMEM        40..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU364062"
FT   TRANSMEM        85..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU364062"
FT   TRANSMEM        121..139
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU364062"
FT   TRANSMEM        145..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU364062"
FT   TRANSMEM        187..209
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU364062"
FT   TRANSMEM        221..239
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU364062"
FT   TRANSMEM        260..287
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU364062"
FT   TRANSMEM        293..311
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU364062"
FT   TRANSMEM        327..348
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU364062"
FT   TRANSMEM        396..417
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU364062"
FT   TRANSMEM        429..451
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU364062"
FT   TRANSMEM        548..569
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU364062"
FT   TRANSMEM        607..625
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU364062"
FT   TRANSMEM        694..713
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU364062"
FT   TRANSMEM        725..742
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU364062"
FT   DOMAIN          75..125
FT                   /note="NADH-Ubiquinone oxidoreductase (complex I) chain 5
FT                   N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00662"
FT   DOMAIN          141..441
FT                   /note="NADH:quinone oxidoreductase/Mrp antiporter membrane
FT                   subunit"
FT                   /evidence="ECO:0000259|Pfam:PF00361"
FT   DOMAIN          448..691
FT                   /note="NADH:ubiquinone/plastoquinone oxidoreductase
FT                   chloroplast chain 5 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF01010"
SQ   SEQUENCE   744 AA;  84227 MW;  EC6542FEA7DCC051 CRC64;
     MEQTYQYAWI IPFLPLPVPM LIGLGLLLFP TATKSLRRMW AFQSVLLLSI VMIFSMNLSI
     QQINSSSVYQ YVWSWIINND FSLEFGYLID PLTSIMSILI TTVGILVLIY SDNYMSYDHG
     YLRFFAYMSF FSTSMLGLVT SSNLIQIYIF WELVGICSYL LIGFWFTRPV AAKACQKAFV
     TNRVGDFGLL LGILGFYWVT GSFEFRNLFQ IFNNLISNNE VNFVFVTLCA ILLFAGAIAK
     SAQFPLHVWL PDAMEGPTPI SALIHAATMV AAGIFLVARL LPLFIVIPHI MNLISLIGII
     TVFLGATLAL AQKDIKRGLA YSTMSQLGYM MLALGMGSYR SALFHLITHA YSKALLFLGS
     GSVIHSMETL VGYCPKKSQN MVLMGGLTKH VPITKTSFLL GTLSLCGIPP LACFWSKDEI
     LNDSWLYSPI FAIIAWSTAG LTAFYMCRIY LLTFEGHLNV HFQNYSGKKN TPFYSISLWG
     KEGSKISNKN FRLVTLLKMK NNGCASFFSN KVYKIDENLR NMIQPFLSIH HFGNTKTYSY
     PYESDNTMLF PILILVLFTL FVGFLGIPFN QDGGYLDILS KWLTPSINLL HKNSNNSIDW
     YEFCKDAVFS VSISSFGILI AFFLYKPVYS SFQNLDLINS FVKIGPKRIF SDKIKNGIYD
     WSYNRGYIDA FYGTFLTVGI RKLAKFAHFF DRRIIDGIPN GVGLMSFFVA EVIKSVGGGR
     ISSYLFFYFS YVSIFLLIYY CLNF
//