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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Shigella boydii serotype 4 (strain Sb227)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50Substrate; via amide nitrogenUniRule annotation1
Binding sitei125ATPUniRule annotation1
Binding sitei171ATPUniRule annotation1
Binding sitei198Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:SBO_0925
OrganismiShigella boydii serotype 4 (strain Sb227)
Taxonomic identifieri300268 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000007067 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000215271 – 262Hydroxyethylthiazole kinaseAdd BLAST262

Structurei

3D structure databases

ProteinModelPortaliQ323B8.
SMRiQ323B8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000114352.
KOiK00878.
OMAiKRPLVHN.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiView protein in InterPro
IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
PANTHERiPTHR44423. PTHR44423. 1 hit.
PfamiView protein in Pfam
PF02110. HK. 1 hit.
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

Q323B8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVDLQSSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM
60 70 80 90 100
VIETEEASQF AAIASALLIN VGTLTQLRAQ SMCAAVEQAK SSQTPWTLDP
110 120 130 140 150
VAVGALDYRR RFCLELLPHK PTAIRGNASE IMALAGIANG GRGVDTTDAA
160 170 180 190 200
ANAIPAAQTL ARETGAIVVV TGEMDYVTDG HRIIGIHGGD PLMTKVVGTG
210 220 230 240 250
CALSAVVAAC CALPGDTLEN VASACHWMKQ AGERAVARSE GPGSFVPHFL
260
DALWQLTQEV QA
Length:262
Mass (Da):27,388
Last modified:December 6, 2005 - v1
Checksum:iAD38843911628158
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000036 Genomic DNA. Translation: ABB65590.1.

Genome annotation databases

EnsemblBacteriaiABB65590; ABB65590; SBO_0925.
KEGGisbo:SBO_0925.

Similar proteinsi

Entry informationi

Entry nameiTHIM_SHIBS
AccessioniPrimary (citable) accession number: Q323B8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome