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Protein

NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic

Gene

ndhF

Organism
Campanula ramosa
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient.UniRule annotation

Catalytic activityi

NAD(P)H + plastoquinone = NAD(P)+ + plastoquinol.UniRule annotationSAAS annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotationSAAS annotation
Biological processTransportUniRule annotation
LigandNADUniRule annotationSAAS annotation, NADPUniRule annotationSAAS annotation, PlastoquinoneUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H-quinone oxidoreductase subunit 5, chloroplasticUniRule annotation (EC:1.6.5.-UniRule annotation)
Alternative name(s):
NADH-plastoquinone oxidoreductase subunit 5UniRule annotation
Gene namesi
Name:ndhFUniRule annotationImported
Encoded oniPlastid; ChloroplastImported
OrganismiCampanula ramosaImported
Taxonomic identifieri40569 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesCampanulaceaeCampanula

Subcellular locationi

  • Plastidchloroplast thylakoid membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei6 – 25HelicalUniRule annotationAdd BLAST20
Transmembranei37 – 64HelicalUniRule annotationAdd BLAST28
Transmembranei84 – 101HelicalUniRule annotationAdd BLAST18
Transmembranei113 – 131HelicalUniRule annotationAdd BLAST19
Transmembranei137 – 158HelicalUniRule annotationAdd BLAST22
Transmembranei179 – 195HelicalUniRule annotationAdd BLAST17
Transmembranei215 – 234HelicalUniRule annotationAdd BLAST20
Transmembranei255 – 276HelicalUniRule annotationAdd BLAST22
Transmembranei282 – 303HelicalUniRule annotationAdd BLAST22
Transmembranei310 – 331HelicalUniRule annotationAdd BLAST22
Transmembranei343 – 365HelicalUniRule annotationAdd BLAST23
Transmembranei385 – 404HelicalUniRule annotationAdd BLAST20
Transmembranei416 – 439HelicalUniRule annotationAdd BLAST24
Transmembranei540 – 561HelicalUniRule annotationAdd BLAST22
Transmembranei597 – 615HelicalUniRule annotationAdd BLAST19
Transmembranei711 – 731HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

ChloroplastUniRule annotationImported, Membrane, Plastid, ThylakoidUniRule annotation

PTM / Processingi

Keywords - PTMi

QuinoneUniRule annotation

Interactioni

Subunit structurei

NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 121Proton_antipo_NInterPro annotationAdd BLAST58
Domaini133 – 434Proton_antipo_MInterPro annotationAdd BLAST302
Domaini440 – 681Proton_antipo_CInterPro annotationAdd BLAST242

Sequence similaritiesi

Belongs to the complex I subunit 5 family.UniRule annotationSAAS annotation

Keywords - Domaini

Transmembrane, Transmembrane helixUniRule annotationSAAS annotation

Family and domain databases

InterProiView protein in InterPro
IPR002128. NADH_UbQ_OxRdtase_chlpt_su5_C.
IPR003945. NADHpl_OxRdtase_5.
IPR018393. NADHpl_OxRdtase_5_subgr.
IPR001750. ND/Mrp_mem.
IPR001516. Proton_antipo_N.
PfamiView protein in Pfam
PF01010. Proton_antipo_C. 1 hit.
PF00361. Proton_antipo_M. 1 hit.
PF00662. Proton_antipo_N. 1 hit.
PRINTSiPR01435. NPOXDRDTASE5.
TIGRFAMsiTIGR01974. NDH_I_L. 1 hit.

Sequencei

Sequence statusi: Fragment.

Q32055-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
WIIPLLPLPV PMLIGLGLLL FPTATKSLRR IWAFHSVLLL SLVMIFSMNL
60 70 80 90 100
SIQQINSSFI YQYLWSWIIN NDFFLEFGSL LDPLTSIMSI LITTVGILVL
110 120 130 140 150
IYSDNYMFHD RGYLRFFAYM SFFSTSMFGL VTSSNLIQIY IFWELVGICS
160 170 180 190 200
YLLIGFWFTR PVAANACEKA FLTNRVADFG LLLGILGFYW LTGSFEFRDL
210 220 230 240 250
FQIFNNFVSN NEGNLFFVTL CALFLFSGAV AKSAQFPLHV WLPDAMEGPT
260 270 280 290 300
PISALMHAAT MVAAGIFLVA RLLPLFRYIP HIMNLIFLIG ILTVLLGATL
310 320 330 340 350
ALAQKDIKRG LAYSTMSQLG YMMLALGMGS YRSALFHLIT HAYSKALLFL
360 370 380 390 400
GSGWVIHSMQ ILVGYSPNKS QNMVLMGGLR KHVPITKMSF LVGTLSLCGI
410 420 430 440 450
PPLACFWSKD EILNDSWLYS PIFALIAWST AGLTGFYMLR IYLLTFEGHL
460 470 480 490 500
NVDLQNYSGQ KNTSIYSVSL WGKERAKVNK IDLPLFTLLT MKNNEGASFF
510 520 530 540 550
SKKLYQINEN TRNINQTFII ICNFPNKNIF AYPYESDNNI LFPILILVVF
560 570 580 590 600
TLFVGFLGIP FNEVILDILS KWLRPSINLL DRNSNNSTDW PKFLKDGVFS
610 620 630 640 650
VSIAYLGIFL AFFLYKPLYS SLTNFDFVNL FVKKNLKILV FDKLINGIYD
660 670 680 690 700
WSYNRGYIDV FYGTYFLGGI RRVSELTDFF DRQVVDGITN GFGLMNFSLG
710 720 730
ESIKSVGGGR ISSYLFFYLF YVSIFFFFYY LL
Length:732
Mass (Da):83,420
Last modified:May 1, 2000 - v2
Checksum:iBD072994B437FAF9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei1Imported1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39387 Genomic DNA. Translation: AAC37448.2.
PIRiT12753.

Similar proteinsi

Entry informationi

Entry nameiQ32055_9ASTR
AccessioniPrimary (citable) accession number: Q32055
Entry historyiIntegrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: May 1, 2000
Last modified: August 30, 2017
This is version 91 of the entry and version 2 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)